We narrowed to 886 results for: nanobody

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pHR_EF1a-->myc-LaG17-tTA synNotch (LA1)

Plasmid

#162230

Purpose

Lentiviral vector for the expression of GFP nanobody LaG17 tTA SynNotch: pHR_EF1a-->myc-LaG17-tTA synNotch (LA1) in mammalian cells

Depositor

Wendell Lim

Article

Toda et al Science. 2020 Oct 16;370(6514):327-331.

Insert

LaG17 GFP nanobody SynNotch tTA

Use

Lentiviral

Expression

Mammalian

Available Since

June 28, 2022

Availability

Academic Institutions and Nonprofits only

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pET51b(+)_EGFP_NbALFA

Plasmid

#136626

Purpose

Plasmid encoding the bacterial expression of NbALFA for ALFA-tag as an EGFP-fusion

Depositor

Kai Johnsson

Article

Farrants et al Nat Methods. 2020 Feb 17. pii: 10.1038/s

Insert

EGFP-NbALFA

Tags

Hisx10 and Strep

Expression

Bacterial

Promoter

T7

Available Since

Feb. 14, 2020

Availability

Academic Institutions and Nonprofits only

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pDG02583

Plasmid

#104129

Purpose

Optimized bacterial expression plasmid of bdNEDP1 protease (Frey and Goerlich, 2014)

Depositor

Dirk Görlich

Article

Pleiner et al J Cell Biol. 2018 Mar 5;217(3):1143-1154

Insert

NEDD8-specific protease 1 (NEDP1)

Tags

14x Histidine tag, Maltose binding protein, and S…

Expression

Bacterial

Available Since

Dec. 21, 2017

Availability

Academic Institutions and Nonprofits only

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pCAG-dGBP1-TagBFP

Plasmid

#80086

Purpose

GFP-dependent dGBP1-TagBFP, for expression in mammalian cells

Depositor

Connie Cepko

Article

Tang et al Elife. 2016 May 20;5. pii: e15312. doi:

Insert

dGBP1-TagBFP

Expression

Mammalian

Promoter

CAG

Available Since

Dec. 1, 2016

Availability

Academic Institutions and Nonprofits only

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pAAV-hSYN-miRFP670nano3-WPRE-hGHpA

Plasmid

#184688

Purpose

Mammalian expression, miRFP670nano3

Depositor

Axel Nimmerjahn, Vladislav Verkhusha

Article

Oliinyk et al Nat Methods. 2022 May 23. pii: 10.1038/s

Insert

miRFP670nano3

Use

AAV

Promoter

hSYN

Available Since

June 23, 2022

Availability

Academic Institutions and Nonprofits only

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nls-mCherry

Plasmid

#108881

Purpose

Expression of nuclear localized mCherry

Depositor

Rob Parton

Article

Ariotti et al PLoS Biol. 2018 Apr 5;16(4):e2005473. do

Insert

nls-mCherry

Use

Can be used for mammalian expression or in vitro …

Expression

Mammalian

Available Since

Dec. 7, 2018

Availability

Academic Institutions and Nonprofits only

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pNIR-Fbactin

Plasmid

#184677

Purpose

NIR-Fb to actin

Depositor

Vladislav Verkhusha

Article

Oliinyk et al Nat Methods. 2022 May 23. pii: 10.1038/s

Insert

NIR-Fbactin

Expression

Mammalian

Promoter

CMV

Available Since

June 23, 2022

Availability

Academic Institutions and Nonprofits only

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pHR_SFFV_LaG17_synNotch_TetRVP64

Plasmid

#79128

Purpose

Lentiviral vector for constitutive expression of the LaG17 GFP nanobody synNotch TetRVP64 Receptor (SFFV promoter)

Depositor

Wendell Lim

Article

Morsut et al Cell. 2016 Feb 11;164(4):780-91. doi: 10

Insert

LaG17 GFP nanobody synNotch TetRVP64

Use

Lentiviral

Available Since

July 26, 2016

Availability

Academic Institutions and Nonprofits only

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pNIR-FbLAG30

Plasmid

#220740

Purpose

NIR-FbLAG30 expression in mammalian cells.

Depositor

Vladislav Verkhusha

Article

Barykina et al Nat Commun. 2024 Sep 6;15(1):7788. doi:

Insert

NIR-FbLAG30

Use

Affinity Reagent/ Antibody

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 9, 2024

Availability

Academic Institutions and Nonprofits only

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pTRE-tdTomato

Plasmid

#50798

Purpose

Expression of tdTomato under the control of the TRE-promoter

Depositor

Connie Cepko

Article

Tang et al Cell. 2013 Aug 15;154(4):928-39. doi: 10

Insert

TREtight driven tdTomato fluorescent protein

Use

Mutli-species

Promoter

TREtight

Available Since

March 20, 2014

Availability

Academic Institutions and Nonprofits only

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pCAG-Fab_8D3_2_H-H6

Plasmid

#176075

Purpose

Express His6 tagged heavy chain of Fab_8D3_2 in mammalian cells

Depositor

Tom Rapoport

Article

Wu et al Proc Natl Acad Sci U S A. 2021 Oct 12;11

Insert

His6 tagged heavy chain of Fab_8D3_2

Tags

His tag

Expression

Mammalian

Promoter

CAG promoter

Available Since

Oct. 12, 2021

Availability

Academic Institutions and Nonprofits only

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pNIR-FbALFA

Plasmid

#184678

Purpose

NIR-Fb to ALFA-tag

Depositor

Vladislav Verkhusha

Article

Oliinyk et al Nat Methods. 2022 May 23. pii: 10.1038/s

Insert

NIR-FbALFA

Expression

Mammalian

Promoter

CMV

Available Since

June 23, 2022

Availability

Academic Institutions and Nonprofits only

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pBMNb-cALFA

Plasmid

#159991

Purpose

For the protein expression with ALFA tag at C-terminus in mammalian cells

Depositor

Motoyuki Hattori

Article

Jin et al Commun Biol. 2021 Mar 19;4(1):366. doi:

Type

Empty backbone

Tags

HRV3C protease cleavage site-ALFA tag-twin Strep …

Expression

Mammalian

Mutation

CMV promoter, WPRE

Available Since

Oct. 21, 2020

Availability

Academic Institutions and Nonprofits only

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pOPINVHH_snap_his

Plasmid

#210407

Purpose

For expression of VHH with C-terminal snap protein and his tag

Depositor

Ray Owens

Article

Eyssen et al Bio Protoc. 2024 Mar 20;14(6):e4962. doi

Type

Empty backbone

Tags

His6

Expression

Bacterial

Promoter

lac

Available Since

March 26, 2024

Availability

Academic Institutions and Nonprofits only

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H2B-mCherry

Plasmid

#108880

Purpose

Expression of human histone2B fused to mCherry (nuclear marker)

Depositor

Rob Parton

Article

Ariotti et al PLoS Biol. 2018 Apr 5;16(4):e2005473. do

Insert

H2B-mCherry

Use

Can be used for mammalian expression or in vitro …

Expression

Mammalian

Available Since

June 28, 2018

Availability

Academic Institutions and Nonprofits only

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pBTSG

Plasmid

#159420

Purpose

Transient expression of proteins with a C-terminal thermostable GFP (TGP) tag in mammalian cells

Depositor

Dianfan Li

Article

Cai et al Commun Biol. 2020 Dec 10;3(1):753. doi:

Insert

thermostable GFP (TGP)

Tags

polyhistidine (poly-His)

Expression

Mammalian

Available Since

Jan. 7, 2021

Availability

Academic Institutions and Nonprofits only

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pB2AR-miRFP670nano3

Plasmid

#184671

Purpose

Beta-2-adrenergic receptor internally tagged with miRFP670nano3

Depositor

Vladislav Verkhusha

Article

Oliinyk et al Nat Methods. 2022 May 23. pii: 10.1038/s

Insert

B2AR-miRFP670nano3

Expression

Mammalian

Promoter

CMV

Available Since

June 24, 2022

Availability

Academic Institutions and Nonprofits only

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mCherry-CaaX(Hras)

Plasmid

#108886

Purpose

Expression of membrane localized mCherry

Depositor

Rob Parton

Article

Ariotti et al PLoS Biol. 2018 Apr 5;16(4):e2005473. do

Insert

mCherry-CaaX(Hras)

Use

Can be used for mammalian expression or in vitro …

Expression

Mammalian

Available Since

June 13, 2018

Availability

Academic Institutions and Nonprofits only

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pBXNH3CA_MBP

Plasmid

#132700

Purpose

expresses Avi-tagged MBP in E.coli, to use as positive control in ELISA

Depositor

Markus Seeger

Article

Zimmermann et al Nat Protoc. 2020 Apr 8. pii: 10.1038/s41

Insert

Maltose binding Protein

Tags

Avi and His

Expression

Bacterial

Promoter

pBAD

Available Since

Oct. 8, 2019

Availability

Academic Institutions and Nonprofits only

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pNIR-FbGFP

Plasmid

#184675

Purpose

NIR-Fb to GFP

Depositor

Vladislav Verkhusha

Article

Oliinyk et al Nat Methods. 2022 May 23. pii: 10.1038/s

Insert

NIR-FbGFP

Expression

Mammalian

Promoter

CMV

Available Since

June 23, 2022

Availability

Academic Institutions and Nonprofits only

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pOPINVHH_his_flag

Plasmid

#210404

Purpose

For expression of VHH with C-terminal his and Flag tags

Depositor

Ray Owens

Article

Eyssen et al Bio Protoc. 2024 Mar 20;14(6):e4962. doi

Type

Empty backbone

Tags

his6-flag

Expression

Bacterial

Promoter

lac

Available Since

March 26, 2024

Availability

Academic Institutions and Nonprofits only

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pNIR-FbmCherry

Plasmid

#184676

Purpose

NIR-Fb to mCherry

Depositor

Vladislav Verkhusha

Article

Oliinyk et al Nat Methods. 2022 May 23. pii: 10.1038/s

Insert

NIR-FbmCherry

Expression

Mammalian

Promoter

CMV

Available Since

June 23, 2022

Availability

Academic Institutions and Nonprofits only

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pOPINVHH_BAP_his

Plasmid

#210402

Purpose

For expression of VHH with C-terminal biotin acceptor peptide and his tag

Depositor

Ray Owens

Article

Eyssen et al Bio Protoc. 2024 Mar 20;14(6):e4962. doi

Type

Empty backbone

Tags

biotin acceptor peptide-his6

Expression

Bacterial

Promoter

lac

Available Since

March 26, 2024

Availability

Academic Institutions and Nonprofits only

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pCDNA3.0_mitoSNAP

Plasmid

#136623

Purpose

Plasmid encoding outer mitochondrial localization SNAP-Tag

Depositor

Kai Johnsson

Article

Farrants et al Nat Methods. 2020 Feb 17. pii: 10.1038/s

Insert

NTOM20-SNAP-Tag

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 16, 2020

Availability

Academic Institutions and Nonprofits only

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pNIR-Fb59H10

Plasmid

#184682

Purpose

NIR-Fb to HIV p24

Depositor

Vladislav Verkhusha

Article

Oliinyk et al Nat Methods. 2022 May 23. pii: 10.1038/s

Insert

NIR-Fb59H10

Expression

Mammalian

Promoter

CMV

Available Since

June 23, 2022

Availability

Academic Institutions and Nonprofits only

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p3E-APEX2-P2A-EGFP

Plasmid

#108896

Purpose

Multisite gateway vector for 3' tagging with APEX2 (includes cytoplasmic GFP reporter)

Depositor

Rob Parton

Article

Ariotti et al PLoS Biol. 2018 Apr 5;16(4):e2005473. do

Insert

APEX2-P2A-EGFP

Use

Multisite gateway

Available Since

Aug. 30, 2018

Availability

Academic Institutions and Nonprofits only

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pBiex-1 Myosin VI HMM_eGFP

Plasmid

#82714

Purpose

Expresses Myosin VI HMM with a C-terminal eGFP.

Depositor

Sivaraj Sivaramakrishnan

Article

Sommese et al Protein Sci. 2016 Aug 18. doi: 10.1002/p

Insert

Myosin VI

Tags

FLAG purification tag and eGFP

Expression

Bacterial and Insect

Promoter

T7 Promoter

Available Since

Nov. 3, 2016

Availability

Academic Institutions and Nonprofits only

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pETSG

Plasmid

#159418

Purpose

Expression of proteins with a C-terminal thermostable GFP (TGP) tag in Escherichia coli

Depositor

Dianfan Li

Article

Cai et al Commun Biol. 2020 Dec 10;3(1):753. doi:

Insert

thermostable GFP (TGP)

Tags

polyhistidine (poly-His)

Expression

Bacterial

Available Since

Jan. 7, 2021

Availability

Academic Institutions and Nonprofits only

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pCS2+_NLS-Tir1-HA

Plasmid

#117716

Purpose

mRNA synthesis of NLS-TIR1-HA for injection

Depositor

Joerg Mansfeld

Article

Daniel et al Nat Commun. 2018 Aug 17;9(1):3297. doi:

Insert

TRANSPORT INHIBITOR RESPONSE 1

Tags

HA and NLS

Expression

Mammalian

Mutation

codon-optimized TIR1 for murine expression

Promoter

SP6

Available Since

Dec. 4, 2018

Availability

Academic Institutions and Nonprofits only

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pUAS-Cre

Plasmid

#50797

Purpose

Expression of Cre recombinase under the control of the UAS-promoter

Depositor

Connie Cepko

Article

Tang et al Cell. 2013 Aug 15;154(4):928-39. doi: 10

Insert

Cre recombinase

Use

Multi-species

Promoter

UAS-hsp70

Available Since

March 20, 2014

Availability

Academic Institutions and Nonprofits only

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