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Addgene

We narrowed to 2,414 results for: NIG

Showing: 851 - 900 of 2414 results
  1. pSMART-Ala2

    Plasmid
    #71326
    Purpose
    NADPH dependent Alanine Dehydrogenase, Low Phosphate Dependent E. coli Expression
    Depositor
    Insert
    NADPH Alanine Dehydrogenase (ald Synthetic)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    D196A, L197R
    Promoter
    insulated waaHp from E. coli
    Available since
    Jan. 31, 2022
    Availability
    Academic Institutions and Nonprofits only
  2. pLyGo-Bs-4

    Plasmid
    #163142
    Purpose
    pLyGo cloning vector for a sequence of interest (LPMO) in B. subtilis. Vector encoding the BatLPMO10 signal peptide and the LyGo cassette (SapI-ccdB-SapI)
    Depositor
    Type
    Empty backbone
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    PamyL-PamyQ-PcryIIIA-cryIIIAstab49
    Available since
    July 30, 2021
    Availability
    Academic Institutions and Nonprofits only
  3. pLyGo-Ec-4

    Plasmid
    #163130
    Purpose
    pLyGo cloning vector for periplasmic expression in E. coli of a sequence of interest (LPMO). Vector encoding PhoA signal peptide and the LyGo cassette (SapI-ccdB-SapI)
    Depositor
    Type
    Empty backbone
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    July 30, 2021
    Availability
    Academic Institutions and Nonprofits only
  4. pLyGo-Ec-1

    Plasmid
    #163127
    Purpose
    pLyGo cloning vector for cytoplasmic expression in E. coli of a sequence of interest (LPMO). Vector encoding Ubiquitin with an internal His10-tag and the LyGo cassette (SapI-ccdB-SapI)
    Depositor
    Type
    Empty backbone
    Use
    Synthetic Biology
    Tags
    Ubiquitin
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    May 20, 2021
    Availability
    Academic Institutions and Nonprofits only
  5. pLyGo-Bs-1

    Plasmid
    #163137
    Purpose
    pLyGo cloning vector for a sequence of interest (LPMO) in Bacillus subtilis. Vector encoding the AmyL signal peptide and the LyGo casette (SapI-ccdB-SapI)
    Depositor
    Type
    Empty backbone
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    PamyL-PamyQ-PcryIIIA-cryIIIAstab49
    Available since
    May 7, 2021
    Availability
    Academic Institutions and Nonprofits only
  6. pLyGo-Bs-3

    Plasmid
    #163141
    Purpose
    pLyGo cloning vector for a sequence of interest (LPMO) in B. subtilis. Vector encoding the AprE signal peptide and the LyGo cassette (SapI-ccdB-SapI)
    Depositor
    Type
    Empty backbone
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    PamyL-PamyQ-PcryIIIA-cryIIIAstab49
    Available since
    May 7, 2021
    Availability
    Academic Institutions and Nonprofits only
  7. pLyGo-Bs-2

    Plasmid
    #163140
    Purpose
    pLyGo cloning vector for a sequence of interest (LPMO) in Bacillus subtilis. Vector encoding the AmyQ signal peptide and the LyGo cassette (SapI-ccdB-SapI)
    Depositor
    Type
    Empty backbone
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    PamyL-PamyQ-PcryIIIA-cryIIIAstab49
    Available since
    May 7, 2021
    Availability
    Academic Institutions and Nonprofits only
  8. pLyGo-Ec-3

    Plasmid
    #163129
    Purpose
    pLyGo cloning vector for periplasmic expression in E. coli of a sequence of interest (LPMO). Vector encoding OmpA signal peptide and the LyGo cassette (SapI-ccdB-SapI)
    Depositor
    Type
    Empty backbone
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    May 6, 2021
    Availability
    Academic Institutions and Nonprofits only
  9. pLyGo-Ec-5

    Plasmid
    #163131
    Purpose
    pLyGo cloning vector for periplasmic expression in E. coli of a sequence of interest (LPMO). Vector encoding DsBA signal peptide and the LyGo cassette (SapI-ccdB-SapI)
    Depositor
    Type
    Empty backbone
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    April 7, 2021
    Availability
    Academic Institutions and Nonprofits only
  10. pMflT-o2

    Plasmid
    #101310
    Purpose
    Contains: oriC2 fragment of M. florum L1 replication origin (dnaA/dnaN intergenic region), colE1 replication origin, recoded tetM resistance gene.
    Insert
    oriC2 of M. florum strain L1 (dnaA/dnaN intergenic region)
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Nov. 21, 2017
    Availability
    Academic Institutions and Nonprofits only
  11. pMflT-o1

    Plasmid
    #101309
    Purpose
    Contains: oriC1 fragment of M. florum L1 replication origin (rpmH/dnaA intergenic region), colE1 replication origin, recoded tetM resistance gene, origin of transfer of RP4 plasmid.
    Insert
    oriC1 of M. florum strain L1 (rpmH/dnaA intergenic region)
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Nov. 7, 2017
    Availability
    Academic Institutions and Nonprofits only
  12. pETDuet-msGC-NT2

    Plasmid
    #74763
    Purpose
    expresses the N-terminal fragment of manduca sexta soluble guanylyl cyclase subunits alpha and beta
    Depositor
    Inserts
    fragment of alfa subunit of sGC
    fragment of beta subunit of sGC
    Use
    Tags
    His-tag
    Expression
    Bacterial
    Mutation
    fragment 49-471 of the alfa subunit
    Promoter
    T7
    Available since
    June 14, 2016
    Availability
    Academic Institutions and Nonprofits only
  13. Tet-pLKO-neo

    Plasmid
    #21916
    Purpose
    3rd generation lentiviral plasmid for inducible expression of shRNA; neomycin selection. See manual for detailed protocols. No further technical support is available from the depositing laboratory.
    Has Service
    Cloning Grade DNA
    Type
    Empty backbone
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Aug. 22, 2009
    Availability
    Academic Institutions and Nonprofits only
  14. pAf-CRISPR-yA

    Plasmid
    #191015
    Purpose
    CRISPR vector based on Aspergillus flavus U6 promoter and terminator for targeting the yA gene, which contains AMA1(the HindIII-PstI fragment) and ptrA selection marker.
    Depositor
    Insert
    yA
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    Aspergillus flavus U6
    Available since
    May 24, 2023
    Availability
    Academic Institutions and Nonprofits only
  15. pCAGGS_NP_EBOV

    Plasmid
    #103049
    Purpose
    Encodes Ebola virus NP gene (Zaire ebolavirus, Mayinga) under control of the CAG promoter
    Depositor
    Insert
    NP gene of Ebola virus (NP Zaire ebolavirus, Mayinga)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    silent mutation (T to C) at position 928 of NP ge…
    Promoter
    CAG
    Available since
    Dec. 20, 2017
    Availability
    Academic Institutions and Nonprofits only
  16. pFlp-Gm

    Plasmid
    #222335
    Purpose
    Expresses Flp and gentamicin to enable excision of antibiotic resistance cassettes; flp-cl-sacB-pL1V-Lv1-gent-pBBR1-ELT3
    Depositor
    Inserts
    sacB (sacB B. subtilis)
    flp (flp Budding Yeast)
    cI (cI )
    Use
    Tags
    Expression
    Mutation
    Promoter
    cl, flp, and sacB
    Available since
    Nov. 7, 2024
    Availability
    Academic Institutions and Nonprofits only
Showing: 851 - 900 of 2414 results