Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

Filter By

More Filters

We narrowed to 24,950 results for: Spr

Clear keywords
Showing: 851 - 900 of 24950 results

  1. pCDNA3-Flag-SPRTN-E112A

    Plasmid
    #110215
    Purpose
    Expression in mammalian cells of of full-length SPRTN protein carrying E112A mutation, a catalytically-dead mutant, and Flag-tag at N-terminus.
    Depositor
    Ivana Marinovic-Terzic, Kristijan Ramadan
    Article
    Lessel et al Nat Genet. 2014 Nov;46(11):1239-44. doi:
    Insert
    SPRTN (SPRTN Human)
    Tags
    Flag
    Expression
    Mammalian
    Mutation
    E112A, P296L (see depositor comments below)
    Available Since
    May 23, 2018
    Availability
    Academic Institutions and Nonprofits only
    110215-plasmid-map-sequence-id-210950

    Enlarge

  3. pYPQ-ABE-SpRYn-Act3.0

    Plasmid
    #178959
    Purpose
    It consists of a SpRY nickase (D10A) fused with an adenine deaminase (ABE8e) and MS2-SunTag-activators (ScFv-sfGFP-2xTAD), enabling simultaneous A to G conversion and gene activation.
    Depositor
    Yiping Qi
    Article
    Pan et al Nat Plants. 2022 May;8(5):513-525. doi:
    Insert
    ABE8e-SpRY-T2A-MS2-SunTag-rbcS-E9t-ter-ZmUbi-scFv-sfGFP-2xTAD-GB1-NOS-ter
    Use
    CRISPR
    Expression
    Plant
    Available Since
    May 23, 2022
    Availability
    Academic Institutions and Nonprofits only
    178959-plasmid-map-sequence-id-354322

    Enlarge

  4. lentiGuide-Puro PRMT5-CRISPRi

    Plasmid
    #164637
    Purpose
    lentiGuide-Puro backbone with cloned target sequence for human PRMT5. The insert is: 5´- CACCGAGCCGCGTGTCCAGCGGGA-3. sgRNA achieves a downregulation of PRMT5 in combination with dCAS9-KRAB-MCP2
    Depositor
    Günter Schneider
    Insert
    target guide sequence PRMT5 inhibition (PRMT5 Human)
    Use
    CRISPR and Lentiviral; Interference
    Expression
    Mammalian
    Promoter
    U6 and EF1A
    Available Since
    May 9, 2022
    Availability
    Academic Institutions and Nonprofits only
    164637-plasmid-map-sequence-id-326103

    Enlarge

  24. R777-E322 Hs.SPRED1-nostop

    Plasmid
    #70606
    Purpose
    Gateway ORF clone of human SPRED1 [NM_152594.2] without stop codon (for C-terminal fusions)
    Depositor
    Dominic Esposito
    Insert
    SPRED1 (SPRED1 Human)
    Use
    Gateway entry clone
    Available Since
    March 1, 2016
    Availability
    Academic Institutions and Nonprofits only
    70606-plasmid-map-sequence-id-126055

    Enlarge

  27. R777-E330 Hs.SPRY2-nostop

    Plasmid
    #70614
    Purpose
    Gateway ORF clone of human SPRY2 [NM_005842.2] without stop codon (for C-terminal fusions)
    Depositor
    Dominic Esposito
    Insert
    SPRY2 (SPRY2 Human)
    Use
    Gateway entry clone
    Available Since
    March 1, 2016
    Availability
    Academic Institutions and Nonprofits only
    70614-plasmid-map-sequence-id-126063

    Enlarge

  28. R777-E334 Hs.SPRY4-nostop

    Plasmid
    #70617
    Purpose
    Gateway ORF clone of human SPRY4 [NM_001127496.1] without stop codon (for C-terminal fusions)
    Depositor
    Dominic Esposito
    Insert
    SPRY4 (SPRY4 Human)
    Use
    Gateway entry clone
    Available Since
    March 1, 2016
    Availability
    Academic Institutions and Nonprofits only
    70617-plasmid-map-sequence-id-133434

    Enlarge

  31. R777-E324 Hs.SPRED2-nostop

    Plasmid
    #70608
    Purpose
    Gateway ORF clone of human SPRED2 [NM_181784.2] without stop codon (for C-terminal fusions)
    Depositor
    Dominic Esposito
    Insert
    SPRED2 (SPRED2 Human)
    Use
    Gateway entry clone
    Available Since
    March 1, 2016
    Availability
    Academic Institutions and Nonprofits only
    70608-plasmid-map-sequence-id-126057

    Enlarge

  32. R777-E328 Hs.SPRY1-nostop

    Plasmid
    #70612
    Purpose
    Gateway ORF clone of human SPRY1 [NM_001258038.1] without stop codon (for C-terminal fusions)
    Depositor
    Dominic Esposito
    Insert
    SPRY1 (SPRY1 Human)
    Use
    Gateway entry clone
    Available Since
    March 1, 2016
    Availability
    Academic Institutions and Nonprofits only
    70612-plasmid-map-sequence-id-126061

    Enlarge

  34. R777-E332 Hs.SPRY3-nostop

    Plasmid
    #70616
    Purpose
    Gateway ORF clone of human SPRY3 [NM_005840.2] without stop codon (for C-terminal fusions)
    Depositor
    Dominic Esposito
    Insert
    SPRY3 (SPRY3 Human)
    Use
    Gateway entry clone
    Available Since
    March 1, 2016
    Availability
    Academic Institutions and Nonprofits only
    70616-plasmid-map-sequence-id-126065

    Enlarge

  38. pCDNA3-Flag-SPRTN-Y117C

    Plasmid
    #110216
    Purpose
    Expression in mammalian cells of of full-length SPRTN protein carrying Y117C mutation, a catalytically-impaired mutant, and Flag-tag at N-terminus.
    Depositor
    Ivana Marinovic-Terzic, Kristijan Ramadan
    Article
    Vaz et al Mol Cell. 2016 Nov 17;64(4):704-719. doi
    Insert
    SPRTN (SPRTN Human)
    Tags
    Flag
    Expression
    Mammalian
    Mutation
    Y117C, P296L (see depositor comments below)
    Available Since
    May 23, 2018
    Availability
    Academic Institutions and Nonprofits only
    110216-plasmid-map-sequence-id-210941

    Enlarge

  40. lentiCRISPR - Amplicon, JAK2 sgRNA 1

    Plasmid
    #70679
    Purpose
    Expresses human codon-optimized Cas9 protein and puromycin resistance from EFS promoter and an intergenic JAK2 amplicon-targeting sgRNA element from U6 promoter. Lentiviral backbone
    Depositor
    David Sabatini
    Article
    Wang et al Science. 2015 Oct 15. pii: aac7041.
    Insert
    sgRNA against JAK2 amplicon found in AML-derived HEL cell line (JAK2 )
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Available Since
    Oct. 29, 2015
    Availability
    Academic Institutions and Nonprofits only

  43. lentiCRISPR - Amplicon, JAK2 sgRNA 2

    Plasmid
    #70660
    Purpose
    Expresses human codon-optimized Cas9 protein and puromycin resistance from EFS promoter and an intergenic JAK2 amplicon-targeting sgRNA element from U6 promoter. Lentiviral backbone
    Depositor
    David Sabatini
    Article
    Wang et al Science. 2015 Oct 15. pii: aac7041.
    Insert
    sgRNA against JAK2 amplicon found in AML-derived HEL cell line (JAK2 )
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Available Since
    Oct. 29, 2015
    Availability
    Academic Institutions and Nonprofits only
Showing: 851 - 900 of 24950 results