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Showing: 851 - 900 of 6893 results

  2. CoV2-Spike-EF1a-D614G-N501Y

    Plasmid
    #177939
    Purpose
    Transient mammalian expression of codon optimized SARS-CoV-2 Spike proteins (D614G, N501Y). This version gives lower expression of Spike and is more convenient for generating VLPs.
    Depositor
    Jennifer Doudna
    Article
    Syed et al Science. 2021 Nov 4:eabl6184. doi: 10.11
    Insert
    SARS-CoV-2 Spike protein (D614G, N501Y) (S )
    Use
    Tags
    Expression
    Mammalian
    Mutation
    D614G, N501Y
    Promoter
    Available since
    Nov. 16, 2021
    Availability
    Academic Institutions and Nonprofits only
    177939-plasmid-map-sequence-id-348111

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  11. qTAG-AAVS1-Ef1a-Puro-mScarlet

    Plasmid
    #227274
    Purpose
    AAVS1 targeting donor for the insertion of Puro and a strong EF1a promoter expressing mScarlet. To be co-transfected with sgRNA plasmid px330-AAVS1 (Addgene #227272)
    Depositor
    Laurence Pelletier
    Article
    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318
    Insert
    AAVS1 Homology Arms flanking a 2A-Puro-EF1a-mScarlet cassette (AAVS1 Synthetic)
    Use
    CRISPR; Donor template
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Promoterless
    Available since
    Nov. 12, 2024
    Availability
    Academic Institutions and Nonprofits only
    227274-plasmid-map-sequence-id-452035

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  12. qTAG-AAVS1-Ef1a-Puro-moxGFP

    Plasmid
    #227273
    Purpose
    AAVS1 targeting donor for the insertion of Puro and a strong EF1a promoter expressing moxGFP. To be co-transfected with sgRNA plasmid px330-AAVS1 (Addgene #227272)
    Depositor
    Laurence Pelletier
    Article
    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318
    Insert
    AAVS1 Homology Arms flanking a 2A-Puro-EF1a-moxGFP cassette (AAVS1 Synthetic)
    Use
    CRISPR; Donor template
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Promoterless
    Available since
    Nov. 12, 2024
    Availability
    Academic Institutions and Nonprofits only
    227273-plasmid-map-sequence-id-452031

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  15. pAAV-Ef1a-DIO C1V1 (t/t)-TS-mCherry

    Plasmid
    #35498
    Purpose
    AAV expression of Ef1a-driven, cre-dependent, chimeric opsin variant C1V1 (E122T/E162T) for fast and potent optical excitation at red-shifted wavelengths.
    Depositor
    Karl Deisseroth
    Article
    Yizhar et al Nature. 2011 Jul 27;477(7363):171-8. doi
    Insert
    ChR1-VChR1 Chimera
    Use
    AAV
    Tags
    mCherry
    Expression
    Mammalian
    Mutation
    E122T and E162T
    Promoter
    Ef1a
    Available since
    April 18, 2012
    Availability
    Academic Institutions and Nonprofits only
    35498-plasmid-map-sequence-id-199355

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  19. LV_EF1a_CDK1(T14A,Y15F)-P2A-Hygro_Barcode

    Plasmid
    #170220
    Purpose
    Barcoded lentiviral vector to express CDK1 (T14A, Y15F) in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq
    Depositor
    Prashant Mali
    Article
    Parekh et al iScience. 2021 Sep 20;24(10):103149. doi
    Insert
    CDK1 (CDK1 Human)
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    T14A, Y15F
    Promoter
    EF1a
    Available since
    Sept. 23, 2021
    Availability
    Academic Institutions and Nonprofits only
    170220-plasmid-map-sequence-id-334386

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  23. pLV-EF1A-CD19.28H.28TM.BB.28.3z

    Plasmid
    #200681
    Purpose
    Modular CD28hinge-CD28transmembrane-41BB-CD28-CD3z CAR backbone, For Transient Expression or Lentiviral Production
    Depositor
    Michele Bernasconi
    Article
    Timpanaro et al J Exp Clin Cancer Res. 2023 Nov 4;42(1):
    Insert
    FMC63-CD28HD-CD28TM-41BB-CD28-CD3z-P2A-EGFP (CD28, CD247, TNFRSF9 Human)
    Use
    Lentiviral
    Tags
    MycTag
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a-short
    Available since
    Nov. 6, 2023
    Availability
    Academic Institutions and Nonprofits only
    200681-plasmid-map-sequence-id-392612

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  35. pAAV-Ef1a-Flex-Axon-GCaMP7b

    Plasmid
    #135419
    Purpose
    Can be used to drive axon-enriched expression of GCaMP7b in the presence of Cre recombinase.
    Depositor
    Rylan Larsen
    Insert
    axon-jGCaMP7b
    Use
    AAV
    Tags
    6xHis and GAP43 palmitoylation domain
    Expression
    Mammalian
    Mutation
    Promoter
    Ef1a
    Available since
    Feb. 5, 2020
    Availability
    Academic Institutions and Nonprofits only
    135419-plasmid-map-sequence-id-271053

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  44. pLV[Exp]-Neo-EF1A>Tet3G

    Plasmid
    #184379
    Purpose
    The Tet3G gene is expressed under a constitutive EF1-alpha promoter. This protein binds a TRE3G promoter to activate gene transcription only in the presence of tetracycline or its analogs (e.g. doxycycline)
    Depositor
    Ori Bar-Nur
    Article
    Domenig et al Stem Cell Reports. 2022 Feb 8;17(2):321-
    Insert
    Tet3G
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    Promoter
    Available since
    May 23, 2022
    Availability
    Academic Institutions and Nonprofits only
    184379-plasmid-map-sequence-id-362511

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  49. pAAV-Ef1a-DIO-PPO-Venus

    Plasmid
    #139505
    Purpose
    AAV vector for Cre-dependent expression of Venus-tagged parapinopsin driven by the Ef1a promoter. Used for photoswitchable control of inhibitory GPCR signaling cascades.
    Depositor
    Michael Bruchas
    Article
    Copits et al Neuron. 2021 Jun 2;109(11):1791-1809.e11
    Has Service
    AAV8 and AAV9
    Insert
    parapinopsin
    Use
    AAV
    Tags
    Venus tag following 3x Ala linker
    Expression
    Mammalian
    Mutation
    Promoter
    Ef1a
    Available since
    Feb. 23, 2021
    Availability
    Academic Institutions and Nonprofits only
    139505-plasmid-map-sequence-id-276175

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Showing: 851 - 900 of 6893 results