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We narrowed to 5,295 results for: pcDNA3.1+

Showing: 851 - 900 of 5295 results

  1. pcDNA3.1-ccdB-TwinStrep

    Plasmid
    #214988
    Purpose
    Gateway compatible empty vector (transient mammalian expression) with a C-terminal TwinStrep tag
    Depositor
    Wade Harper
    Type
    Empty backbone
    Use
    Tags
    TwinStrep
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    March 15, 2024
    Availability
    Academic Institutions and Nonprofits only
    214988-plasmid-map-sequence-id-440022

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  6. pcDNA3.1-MS2-HA-HshnRNPK_Z

    Plasmid
    #148124
    Purpose
    Mammalian Expression of HshnRNPK
    Depositor
    Elisa Izaurralde
    Insert
    HshnRNPK (HNRNPK Human)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    March 16, 2023
    Availability
    Academic Institutions and Nonprofits only
    148124-plasmid-map-sequence-id-473834

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  11. pcDNA3.1-ccdB-Firefly

    Plasmid
    #87066
    Purpose
    Mammalian Gateway-compatible expression vector backbone with a C-terminal Firefly luciferase
    Depositor
    Mikko Taipale
    Type
    Empty backbone
    Use
    Tags
    Firefly luciferase
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Aug. 16, 2017
    Availability
    Academic Institutions and Nonprofits only
    87066-plasmid-map-sequence-id-190852

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  12. pcDNA3.1-MAP3K5 (R256C)

    Plasmid
    #47105
    Purpose
    Expresses mutant (R256C) human MAP3K5 in mammalian cells
    Depositor
    Yardena Samuels
    Insert
    MAP3K5 (MAP3K5 Human)
    Use
    Tags
    FLAG
    Expression
    Mammalian
    Mutation
    changed arginine 256 to cysteine
    Promoter
    CMV
    Available since
    Oct. 31, 2013
    Availability
    Academic Institutions and Nonprofits only

  13. pcDNA3.1-mPCSK3 H194L

    Plasmid
    #122689
    Purpose
    Expresses mutated mouse PCSK3 in mammalian cells (histidine at position 194 mutated to leucine via site directed mutagenesis)
    Depositor
    Liming Pei
    Article
    Li et al Mol Cell Biol. 2018 Oct 15;38(21). pii:
    Insert
    Mus musculus furin (paired basic amino acid cleaving enzyme) (Furin), transcript variant 1 (Furin Mouse)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Changed Histidine 194 to Leucine
    Promoter
    Available since
    May 16, 2019
    Availability
    Academic Institutions and Nonprofits only
    122689-plasmid-map-sequence-id-244742

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  14. NLS-4LNDDE488Q-pCDNA3.1(+)

    Plasmid
    #172314
    Purpose
    Expresses ADAR2 Deaminase Domain (DD) hyperactive mutant E488Q fused with 4 repeats of lambda N peptide (4LN) with a Nuclear Localization Signal (NLS)
    Depositor
    Joshua Rosenthal
    Article
    Vallecillo-Viejo et al RNA Biol. 2018 Jan 2;15(1):104-114. doi:
    Insert
    Lambda N peptide - human ADAR2 Deaminase Domain E488Q
    Use
    Tags
    6xHis, Flag, and Nuclear Localization Signal of S…
    Expression
    Mammalian
    Mutation
    changed the Glutamic acid 488 in the Deaminase Do…
    Promoter
    CMV
    Available since
    Aug. 17, 2021
    Availability
    Academic Institutions and Nonprofits only
    172314-plasmid-map-sequence-id-339830

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  15. pcDNA3.1-mPCSK3 S368A

    Plasmid
    #122680
    Purpose
    Expresses mutated mouse PCSK3 in mammalian cells (serine at position 368 mutated to alanine via site directed mutagenesis)
    Depositor
    Liming Pei
    Article
    Li et al Mol Cell Biol. 2018 Oct 15;38(21). pii:
    Insert
    Mus musculus furin (paired basic amino acid cleaving enzyme) (Furin), transcript variant 1 (Furin Mouse)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    changed Serine 368 to Alanine
    Promoter
    Available since
    May 16, 2019
    Availability
    Academic Institutions and Nonprofits only
    122680-plasmid-map-sequence-id-244743

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  16. pcDNA3.1-CnA-H151A

    Plasmid
    #191956
    Purpose
    Expression of phosphatase dead mutant of calcineurin A (CnA-H151A).
    Depositor
    Luca Scorrano
    Insert
    Calcineurin subunit alpha H151A
    Use
    Tags
    Expression
    Mammalian
    Mutation
    H151A
    Promoter
    Available since
    Dec. 14, 2022
    Availability
    Academic Institutions and Nonprofits only
    191956-plasmid-map-sequence-id-379808

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  17. pY130 (pcDNA3.1-U6-huBsCpf1)

    Plasmid
    #92306
    Purpose
    Expression of humanized BsCpf1 and crRNA
    Depositor
    Feng Zhang
    Insert
    huBsCpf1
    Use
    Tags
    NLS and NLS-3xHA
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Oct. 26, 2017
    Availability
    Academic Institutions and Nonprofits only
    92306-plasmid-map-sequence-id-181649

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  18. pcDNA3.1-MS2-HA-HshnRNPE2iso3_Z

    Plasmid
    #148118
    Purpose
    Mammalian Expression of HshnRNPE2iso3
    Depositor
    Elisa Izaurralde
    Insert
    HshnRNPE2iso3 (PCBP2 Human)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    one non silent mutation L239 to S compapred to th…
    Promoter
    Available since
    March 16, 2023
    Availability
    Academic Institutions and Nonprofits only
    148118-plasmid-map-sequence-id-473831

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  26. pcDNA3.1 myc-PITPbeta

    Plasmid
    #58278
    Purpose
    mammalian expression of rat PITP beta
    Depositor
    Shamshad Cockcroft
    Insert
    PITP beta (Pitpnb Rat)
    Use
    Tags
    myc
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    July 15, 2014
    Availability
    Academic Institutions and Nonprofits only

  30. pcDNA3.1 Flag-Eya2

    Plasmid
    #49264
    Purpose
    expresses Flag tagged Eya2
    Depositor
    Heide Ford
    Insert
    Eya2 (EYA2 Human)
    Use
    Tags
    FLAG
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Nov. 5, 2013
    Availability
    Academic Institutions and Nonprofits only

  31. pY128 (pcDNA3.1-U6-huMb2Cpf1)

    Plasmid
    #92304
    Purpose
    Expression of humanized Mb2Cpf1 and crRNA
    Depositor
    Feng Zhang
    Insert
    huMb2Cpf1
    Use
    Tags
    NLS and NLS-3xHA
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Oct. 26, 2017
    Availability
    Academic Institutions and Nonprofits only
    92304-plasmid-map-sequence-id-181652

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  35. pcDNA3.1-Firefly-ccdB

    Plasmid
    #87069
    Purpose
    Mammalian Gateway-compatible expression vector backbone with an N-terminal Firefly luciferase
    Depositor
    Mikko Taipale
    Type
    Empty backbone
    Use
    Tags
    Firefly luciferase
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    March 7, 2017
    Availability
    Academic Institutions and Nonprofits only
    87069-plasmid-map-sequence-id-307652

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  38. pcDNA3.1(+)-FluoSTEP-JNKAR

    Plasmid
    #181857
    Purpose
    Genetically encoded FRET-based sensor for monitoring JNK kinase activity near a protein of interest. Must pair with GFP11-tagged POI to reconstitute donor fluorescence.
    Depositor
    Jin Zhang
    Article
    Tenner et al Sci Adv. 2021 May 21;7(21). pii: 7/21/ea
    Insert
    FluoSTEP-JNKAR
    Use
    Tags
    mRuby2 and sfGFP(1-10)
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    June 2, 2022
    Availability
    Academic Institutions and Nonprofits only
    181857-plasmid-map-sequence-id-363201

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  39. pcDNA3.1(+)-FluoSTEP-AktAR

    Plasmid
    #181856
    Purpose
    Genetically encoded FRET-based sensor for monitoring Akt activity near a protein of interest. Must pair with GFP11-tagged POI to reconstitute donor fluorescence.
    Depositor
    Jin Zhang
    Article
    Tenner et al Sci Adv. 2021 May 21;7(21). pii: 7/21/ea
    Insert
    FluoSTEP-AktAR
    Use
    Tags
    mRuby2 and sfGFP(1-10)
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    May 26, 2022
    Availability
    Academic Institutions and Nonprofits only
    181856-plasmid-map-sequence-id-362780

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  40. pY129 (pcDNA3.1-U6-huMb3Cpf1)

    Plasmid
    #92305
    Purpose
    Expression of humanized Mb3Cpf1 and crRNA
    Depositor
    Feng Zhang
    Insert
    huMb3Cpf1
    Use
    Tags
    NLS and NLS-3xHA
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Oct. 26, 2017
    Availability
    Academic Institutions and Nonprofits only
    92305-plasmid-map-sequence-id-181650

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  44. pcDNA3.1-hFluc-V5-6xbsMS2_U

    Plasmid
    #147639
    Purpose
    Mammalian Expression of 6xbsMS2
    Depositor
    Elisa Izaurralde
    Insert
    6xbsMS2
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    March 16, 2023
    Availability
    Academic Institutions and Nonprofits only
    147639-plasmid-map-sequence-id-391205

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  46. pY127 (pcDNA3.1-U6-huTsCpf1)

    Plasmid
    #92303
    Purpose
    Expression of humanized TsCpf1 and crRNA
    Depositor
    Feng Zhang
    Insert
    huTsCpf1
    Use
    Tags
    NLS and NLS-3xHA
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Oct. 26, 2017
    Availability
    Academic Institutions and Nonprofits only
    92303-plasmid-map-sequence-id-181636

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  50. pcDNA3.1(-)-mRho.V5.mER.hOr47a

    Plasmid
    #126472
    Purpose
    Human codon-optimized version of D. melanogaster Or47a (hOr47a) with N-terminal tags derived from human Rhodopsin (mRho) and HCN1 (mER) for improved intracellular trafficking in mammalian cells
    Depositor
    Bill Hansson
    Article
    Miazzi et al Chem Senses. 2019 Oct 26;44(9):673-682.
    Insert
    Or47a (Or47a Fly)
    Use
    Tags
    H. sapiens HCN1 106VNKFSL111 (mER), H. sapiens Rh…
    Expression
    Mammalian
    Mutation
    codon optimization for H. sapiens
    Promoter
    CMV
    Available since
    June 13, 2019
    Availability
    Academic Institutions and Nonprofits only
    126472-plasmid-map-sequence-id-246570

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Showing: 851 - 900 of 5295 results