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We narrowed to 5,097 results for: puc

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Showing: 851 - 900 of 5097 results

  13. pUCIDT-attL1-Worm ABeta-attR5

    Plasmid
    #160437
    Purpose
    Entry vector for cloning nematode-codon-optimized Amyloid Beta(1-42) using 2-fragment gateway recombination
    Depositor
    Nicholas Tolwinski
    Article
    Kaur et al Bio Protoc. 2020 Dec 5;10(23):e3856. doi
    Insert
    Homo sapiens amyloid beta precursor protein (APP Nematode, Human)
    Tags
    Cleavage signal
    Expression
    Bacterial
    Mutation
    Codon-optimized for expression in C. elegans
    Available Since
    Oct. 6, 2021
    Availability
    Academic Institutions and Nonprofits only
    160437-plasmid-map-sequence-id-314980

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  20. pUC19-U6-[BsmBI_pegRNA_entry]-tevopreQ1-term (LM1138)

    Plasmid
    #223137
    Purpose
    pU6 entry plamid for cloning prime editor tevopreQ1 epegRNAs (requires BsmBI or Esp3I digest and ligation of duplexed oligos)
    Depositor
    Benjamin Kleinstiver
    Article
    Silverstein et al Nature. 2025 Apr 22. doi: 10.1038/s41586
    Insert
    etevopreQ1 entry plasmid backbone for epegRNA expression via human U6 promoter
    Use
    CRISPR
    Expression
    Mammalian
    Promoter
    U6
    Available Since
    April 23, 2025
    Availability
    Academic Institutions and Nonprofits only
    223137-plasmid-map-sequence-id-447707

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  41. pUC57 DYSF, alt exon 1, 5a, 40a

    Plasmid
    #87889
    Purpose
    research of alternative isoforms of the protein dysferlin
    Depositor
    Jain Foundation
    Insert
    DYSF, alt exon 1, 5a, 40a (DYSF Human)
    Expression
    Bacterial
    Mutation
    alternative isoforms, see comments
    Available Since
    March 21, 2017
    Availability
    Academic Institutions and Nonprofits only
    87889-plasmid-map-sequence-id-174216

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  44. pUC19-U6-BsmBI_cassette-CjeCas9-sgRNA (KAC482)

    Plasmid
    #133793
    Purpose
    U6 promoter sgRNA entry vector used for all CjeCas9 sgRNAs (clone spacer oligos into BsmBI cassette) - similar to V2-TGAA linker sgRNA architecture from Kim et al. Nature Communications 2017
    Depositor
    Joseph Bondy-Denomy, Benjamin Kleinstiver
    Article
    Mahendra et al Nat Microbiol. 2020 Apr;5(4):620-629. do
    Insert
    CjeCas9 sgRNA entry vector
    Expression
    Mammalian
    Mutation
    V2-TGAA linker sgRNA architecture from Kim et al.…
    Promoter
    U6
    Available Since
    March 30, 2020
    Availability
    Academic Institutions and Nonprofits only
    133793-plasmid-map-sequence-id-268077

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  50. pUC57 DYSF, alt exon 1a, 5a, 40a

    Plasmid
    #87897
    Purpose
    research of alternative isoforms of the protein dysferlin
    Depositor
    Jain Foundation
    Insert
    DYSF, alt exon 1a, 5a, 40a (DYSF Human)
    Expression
    Bacterial
    Mutation
    alternative isoforms, see comments
    Available Since
    March 22, 2017
    Availability
    Academic Institutions and Nonprofits only
    87897-plasmid-map-sequence-id-174248

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Showing: 851 - 900 of 5097 results