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Plasmid#195608PurposeExpresses PI-SceI (VDE) homing endonuclease/intein from the T7 promoter that contains the D218A active site mutationDepositorInsertPI-SceI
UseTagsExpressionBacterialMutationT928C, T929C, A942CPromoterT7Available sinceFeb. 1, 2023AvailabilityAcademic Institutions and Nonprofits only -
pT7PI-Sce ESARC D326A
Plasmid#195609PurposeExpresses PI-SceI (VDE) homing endonuclease/intein from the T7 promoter that contains the D326A active site mutationDepositorInsertPI-SceI
UseTagsExpressionBacterialMutationT3593C, A3601C, T3602GPromoterT7Available sinceFeb. 1, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGEX-SIRPT1-Sarcophilus harrisii
Plasmid#75320PurposeGST fusion Sarcophilus harrisii Sacsin 90-1369DepositorInsertSacsin Sarcophilus harrisii 90-1369
UseTagsGST-TagExpressionBacterialMutationPromoterAvailable sinceJuly 18, 2016AvailabilityAcademic Institutions and Nonprofits only -
pColdII-SIRPT1-Sarcophilus harrisii
Plasmid#75352PurposeNt His-tag Sarcophilus harrisii Sacsin 90-1369DepositorInsertSacsin Sarcophilus harrisii 90-1369
UseTagsHis-TagExpressionBacterialMutationPromoterAvailable sinceJuly 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
jk100 pSinEGdsp(p1:MAPPnL;p2:gfp:barcodelandingpad:4xboxB)
Plasmid#79785Purposegenomic Sindbis plasmid, encoding MAPPnL and a GFP RNA with a barcode landing padDepositorInsertMAPP-nL
UseSynthetic Biology; Sindbis virus genomic plasmidTagsExpressionMammalianMutationPromoterAvailable sinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pFAST-BAC BAF155/SMARCC1-His
Plasmid#177863PurposeTransfer vector to generate recombinant baculovirus expressing BAF155/SMARCC1-HisDepositorInsertSMARCC1 (SMARCC1 Human)
UseTagsHis and His tag at C-terminus with GGGGS linkerExpressionInsectMutationPromoterpolyhedrinAvailable sinceDec. 3, 2021AvailabilityAcademic Institutions and Nonprofits only -
pSF143-wArchon1-KGC-EGFP-ER2
Plasmid#108429PurposeCodon-optimized Archon1 fluorescent voltage reporter in C.elegans expression plasmidDepositorInsertwArchon1-KGC-EGFP-ER2
UseTagsExpressionWormMutationPromoterflp-18Available sinceApril 10, 2018AvailabilityAcademic Institutions and Nonprofits only -
LV_EF1a_FLAG-Rheb(Q64L)-P2A-Hygro_Barcode
Plasmid#170229PurposeBarcoded lentiviral vector to express Rheb (Q64L) in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seqDepositorInsertRHEB (RHEB Human)
UseLentiviralTagsFLAGExpressionMutationQ64LPromoterEF1aAvailable sinceSept. 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
LV_EF1a_CDK1(T14A,Y15F)-P2A-Hygro_Barcode
Plasmid#170220PurposeBarcoded lentiviral vector to express CDK1 (T14A, Y15F) in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seqDepositorInsertCDK1 (CDK1 Human)
UseLentiviralTagsExpressionMutationT14A, Y15FPromoterEF1aAvailable sinceSept. 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CaMKIIa-eArch 3.0-EYFP
Plasmid#35516PurposeAAV expression of CaMKIIa-driven Arch 3.0 for optical inhibition.DepositorInsertArch3.0-EYFP
UseAAVTagsEYFPExpressionMammalianMutationEnhanced trafficking signal and ER export signal …PromoterCaMKIIaAvailable sinceApril 18, 2012AvailabilityAcademic Institutions and Nonprofits only -
pFAST-BAC BAF57/SMARCE1-His
Plasmid#177861PurposeTransfer vector to generate recombinant baculovirus expressing BAF57/SMARCE1-His proteinDepositorInsertSMARCE1 (SMARCE1 Human)
UseTagsHis tag at C-terminus with GGGGS linkerExpressionInsectMutationPromoterpolyhedrinAvailable sinceDec. 3, 2021AvailabilityAcademic Institutions and Nonprofits only -
AAV-Syn-FLEX-rc [SomArchon-GFP]
Plasmid#153533PurposeAAV-mediated expression of SomArchon-GFP under the Syn promoter, in floxed/reversed (Cre-dependent) manner.DepositorInsertSomArchon-GFP
UseAAVTagsER2, GFP, and KGCExpressionMammalianMutationPromoterSynAvailable sinceFeb. 1, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-CAG-sbGLuc-hGtARC2-EYFP
Plasmid#114111Purposefusion protein of Gaussia luciferase variant slow burn, Guillardia theta anion channelrhodopsin-2, and enhanced yellow fluorescent protein for bioluminescent optogeneticsDepositorInsertGaussia luciferase, slow burn; Guillardia theta anion channelrhodopsin-2, human codon optimized
UseTagsExpressionMammalianMutationPromoterCAGAvailable sinceAug. 9, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CamKII-ArchT-GFP (PV2527)
Plasmid#99039PurposeAAV-mediated expression of ArchT under the CamKII promoterDepositorHas ServiceAAV Retrograde, AAV1, and AAV5InsertArchT-GFP
UseAAVTagsGFPExpressionMammalianMutationPromoterCamKIIAvailable sinceJan. 31, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLenti-CaMKIIa-eArchT 3.0-EYFP
Plasmid#35513PurposeLentiviral expression of CaMKIIa-driven ArchT 3.0 for optical inhibition.DepositorInserteArchT 3.0-EYFP
UseLentiviralTagsEYFPExpressionMammalianMutationEnhanced trafficking signal and ER export signal …PromoterCaMKIIaAvailable sinceMay 2, 2012AvailabilityAcademic Institutions and Nonprofits only -
LV_EF1a_FLAG-Mkk7-JNK2-P2A-Hygro_Barcode
Plasmid#170228PurposeBarcoded lentiviral vector to express Mkk7-JNK2 fusion in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seqDepositorUseLentiviralTagsFLAGExpressionMutationFusionPromoterEF1aAvailable sinceSept. 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
pPB CAG SMARCA4-IRES-EGFP
Plasmid#153948PurposepiggyBac transposon vector with CAG promoter expressing wild-type SMARCA4 (FLAG-tagged) and EGFPDepositorInsertSMARCA4 (SMARCA4 Human)
UseTagsFLAGExpressionMammalianMutationPromoterCAGAvailable sinceAug. 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
LV_EF1a_MEK1(S218D,S222D)-P2A-Hygro_Barcode
Plasmid#170240PurposeBarcoded lentiviral vector to express MEK1 (S218D, S222D) in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seqDepositorInsertMAP2K1 (MAP2K1 Human)
UseLentiviralTagsExpressionMutationS218D, S222DPromoterEF1aAvailable sinceSept. 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
pPB CAG SMARCA4 T910M-Venus
Plasmid#153951PurposepiggyBac transposon vector with CAG promoter expressing SMARCA4 (T910M mutant)-Venus fusion proteinDepositorInsertSMARCA4 (SMARCA4 Human)
UseTagsVenusExpressionMammalianMutationT910MPromoterCAGAvailable sinceAug. 3, 2020AvailabilityAcademic Institutions and Nonprofits only -
Lenti-sgRNA(MS2)-puro-barcode
Plasmid#89493PurposeCloning backbone for sgRNA, also has a barcode that allows detection from scRNA-seqDepositorInsertsgRNA (with MS2 loop)
UseLentiviralTagsExpressionMutationPromoterU6Available sinceJune 30, 2017AvailabilityAcademic Institutions and Nonprofits only