We narrowed to 5,784 results for: pcas

1. pCas9-CAT

   Plasmid

   #80323

   Purpose

   Encodes Cas9 and chloramphenicol acetyltransferase (CAT)

   Depositor

   Sebastian Lourido

   Article

   Sidik et al Cell. 2016 Sep 8;166(6):1423-1435.e12. d

   Inserts

   Cas9
   Chloramphenicol acetyltransferase
   

   Use

   CRISPR

   Tags

   3X FLAG and NLS

   Promoter

   TgTUB1

   Available Since

   Sept. 12, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCAFNF-GFP

   Plasmid

   #13772

   Purpose

   Flp recombinase-dependent expression of GFP, i.e. GFP is only expressed in the presence of Flp due to a transcriptional stop in between the FRT sites.

   Depositor

   Connie Cepko

   Article

   Matsuda et al Proc Natl Acad Sci U S A. 2007 Jan 16;10

   Insert

   EGFP
   

   Use

   Flp/frt

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   April 20, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCAG-Eco

   Plasmid

   #35617

   Depositor

   Arthur Nienhuis, Patrick Salmon

   Insert

   Ecotropic MLV envelope
   

   Expression

   Mammalian

   Available Since

   July 16, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCAG-CreERT2

   Plasmid

   #14797

   Purpose

   Mammalian expression of conditionally active form of Cre recombinase (not as tightly regulated as pCAG-ERT2CreERT2)

   Depositor

   Connie Cepko

   Article

   Matsuda et al Proc Natl Acad Sci U S A. 2007 Jan 16;10

   Insert

   Cre-ERT2 fusion protein
   

   Use

   Cre/Lox

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   April 20, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCAG-VSVG

   Plasmid

   #64084

   Purpose

   Expression vector for Vesicular Stomatitis Virus Indiana strain glycoprotein gene

   Depositor

   Ian Wickersham

   Insert

   VSV glycoprotein
   

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   April 15, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCA24N-ligase

   Plasmid

   #87741

   Purpose

   IPTG-inducible expression of T4 DNA ligase for protein purification

   Depositor

   Wayne Patrick

   Article

   Wilson et al Protein Eng Des Sel. 2013 Jul;26(7):471-

   Insert

   T4 DNA ligase (30 )
   

   Tags

   6xHis

   Expression

   Bacterial

   Promoter

   T5-lac

   Available Since

   March 21, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCAG-FlpO

   Plasmid

   #89574

   Purpose

   Expresses FlpO under pCAG

   Depositor

   Wilson Wong

   Article

   Weinberg et al Nat Biotechnol. 2017 Mar 27. doi: 10.103

   Insert

   FlpO
   

   Use

   Cre/Lox and Synthetic Biology

   Tags

   NLS

   Expression

   Mammalian

   Available Since

   April 26, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCAG-mTagBFP2

   Plasmid

   #122373

   Purpose

   Expresses mTagBFP2 in mammalian cells

   Depositor

   Hiroshi Ochiai

   Article

   Ochiai et al Sci Adv. 2020 Jun 17;6(25):eaaz6699. doi

   Insert

   mTagBFP2
   

   Expression

   Mammalian

   Available Since

   June 30, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCas9-polyA

   Plasmid

   #72602

   Purpose

   express hCas9 byT7 promoter with polyadenine tails

   Depositor

   Tomoji Mashimo

   Article

   Yoshimi et al Nat Commun. 2016 Jan 20;7:10431. doi: 10

   Insert

   Cas9-polyadenine
   

   Tags

   81-bp polyadenylation

   Promoter

   T7

   Available Since

   July 15, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. pET-21a_3xNLS_SpCas9_protein_expression

    Plasmid

    #114365

    Purpose

    Protein expression plasmid for 3xNLS SpCas9 in pET-21a backbone

    Depositor

    Scot Wolfe

    Article

    Wu et al Nat Med. 2019 May;25(5):776-783. doi: 10

    Insert

    3xNLS_SpCas9
    

    Use

    CRISPR

    Tags

    NLS

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    April 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCAG-TetON-3G

    Plasmid

    #96963

    Purpose

    Mammalian expression of Clontech rtTA driven by human beta-actin promoter

    Depositor

    Elena Cattaneo

    Article

    Faedo et al Proc Natl Acad Sci U S A. 2017 Feb 14;11

    Type

    Empty backbone

    Expression

    Mammalian

    Available Since

    Aug. 1, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCAG G-CEPIA1er

    Plasmid

    #105012

    Purpose

    ER calcium sensor

    Depositor

    Franck Polleux

    Article

    Hirabayashi et al Science. 2017 Nov 3;358(6363):623-630. d

    Insert

    G-CEPIA1er
    

    Expression

    Mammalian

    Promoter

    pCAG

    Available Since

    Jan. 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pSpCas9 (PX165)

    Plasmid

    #48137

    Purpose

    Human codon optimized Cas9 nuclease from Streptococcus pyogenes (Cbh-3X-FLAG-NLS-SpCas9-NLS)

    Depositor

    Feng Zhang

    Article

    Ran et al Nat Protoc. 2013 Nov;8(11):2281-308. doi

    Insert

    hSpCas9
    

    Use

    CRISPR

    Tags

    3XFLAG

    Expression

    Mammalian

    Promoter

    Cbh

    Available Since

    Oct. 29, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCAG-iCre

    Plasmid

    #89573

    Purpose

    Expresses iCre under pCAG

    Depositor

    Wilson Wong

    Article

    Weinberg et al Nat Biotechnol. 2017 Mar 27. doi: 10.103

    Insert

    iCre
    

    Use

    Cre/Lox and Synthetic Biology

    Tags

    NLS

    Expression

    Mammalian

    Available Since

    April 27, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCambia-PUP-IT

    Plasmid

    #186478

    Purpose

    Expresses POI-PUP-IT fusion in Plants

    Depositor

    Jen Sheen

    Article

    Ye et al Nature. 2022 Sep 14. pii: 10.1038/s41586

    Insert

    FLAG-Pup(E)
    

    Tags

    Triple Flag Tag

    Expression

    Plant

    Promoter

    35S

    Available Since

    Sept. 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCAGGs-RFP

    Plasmid

    #127347

    Purpose

    Expresses RFP constitutively via the CAGGS promoter in mammalian cells

    Depositor

    Phil Sharp

    Insert

    Red Fluorescent Protein
    

    Expression

    Mammalian

    Promoter

    CAGGS Promoter

    Available Since

    Dec. 10, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCas9-sgAAVS1-2

    Plasmid

    #129727

    Purpose

    Expressing SpCas9 and sgAAVS1-2

    Depositor

    Elina Ikonen

    Article

    Li et al Nat Methods. 2019 Aug 26. pii: 10.1038/s

    Insert

    SpCas9 and sgAAVS1-2
    

    Use

    CRISPR

    Tags

    T2A-mCherry-P2A-Puro

    Expression

    Mammalian

    Promoter

    hU6

    Available Since

    Aug. 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCAG-Cre:GFP

    Plasmid

    #13776

    Purpose

    Mammalian expression of Cre recombinase fused to GFP

    Depositor

    Connie Cepko

    Article

    Matsuda et al Proc Natl Acad Sci U S A. 2007 Jan 16;10

    Insert

    Cre-GFP fusion protein
    

    Use

    Cre/Lox

    Tags

    GFP

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    April 20, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCAG-YFP

    Plasmid

    #11180

    Purpose

    Mammalian expression vector for expression of YFP

    Depositor

    Connie Cepko

    Article

    Matsuda et al Proc Natl Acad Sci U S A. 2004 Jan 6. 10

    Insert

    EYFP from Aequorea victoria
    

    Expression

    Mammalian

    Available Since

    April 13, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCAD29_hIRG1_4-461_pvp008

    Plasmid

    #124843

    Purpose

    Expresses human CAD (Irg1) in E.coli

    Depositor

    Konrad Buessow

    Article

    Chen et al Proc Natl Acad Sci U S A. 2019 Sep 23. p

    Insert

    cis-aconitate decarboxylase (ACOD1 Human)
    

    Tags

    StrepTag and TEV site

    Expression

    Bacterial

    Mutation

    deleted amino acids 1-3 and 462-end.

    Promoter

    T7

    Available Since

    Sept. 16, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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