We narrowed to 10,694 results for: AGA

1. Lenti-sgTsc1#1/Cre

   Plasmid

   #193246

   Purpose

   Lentiviral vector expressing Cre recombinase (driven by mPGK promoter) and an sgRNA against the mouse Tsc1 gene

   Depositor

   Julien Sage

   Article

   Lee et al Cell Rep. 2023 Jan 13;42(1):111990. doi:

   Insert

   sgTsc1#1 (Tsc1 Mouse)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Dec. 12, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. Lenti-sgZfp536#2/Cre

   Plasmid

   #193249

   Purpose

   Lentiviral vector expressing Cre recombinase (driven by mPGK promoter) and an sgRNA against the mouse Zfp536 gene

   Depositor

   Julien Sage

   Article

   Lee et al Cell Rep. 2023 Jan 13;42(1):111990. doi:

   Insert

   sgZfp536#2 (Zfp536 Mouse)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Dec. 12, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pAc5-STABLE2-neo-RvCAHS3

   Plasmid

   #192472

   Purpose

   Stable expression of RvCAHS3 (fly codon optimized) in Drosophila cells

   Depositor

   Takekazu Kunieda

   Article

   Tanaka et al PLoS Biol. 2022 Sep 6;20(9):e3001780. do

   Insert

   CAHS3
   

   Use

   Tags

   T2A-EGFP-T2A-neoR

   Expression

   Insect

   Mutation

   Promoter

   Ac5 promoter

   Available since

   Dec. 1, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLV-GG-hUbC-EBPF2-gRNA-DSP

   Plasmid

   #185549

   Purpose

   Lentiviral backbone with EBFP2 reporter and gRNA targeting DSP

   Depositor

   Andrew Wilson

   Article

   Werder et al Sci Adv. 2022 Jul 15;8(28):eabo6566. doi

   Insert

   DSP gRNAs
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 1, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. KTK_219

   Plasmid

   #180534

   Purpose

   Entry vector containing K.rhaeticus native promoter pCmcAX (bcs1) (200 bases upstream from gene start codon)

   Depositor

   Tom Ellis

   Article

   Goosens et al ACS Synth Biol. 2021 Dec 17;10(12):3422-

   Insert

   K.rhaeticus native promoter pCmcAX (bcs1) (200 bases upstream from gene start codon)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Aug. 17, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. KTK_223

   Plasmid

   #180538

   Purpose

   Entry vector containing K.rhaeticus native promoter pHxu (200 bases upstream from gene start codon)

   Depositor

   Tom Ellis

   Article

   Goosens et al ACS Synth Biol. 2021 Dec 17;10(12):3422-

   Insert

   K.rhaeticus native promoter pHxu (200 bases upstream from gene start codon)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Aug. 17, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. ppil2.280.457.Y389H

   Plasmid

   #137658

   Purpose

   Expresses N-terminal (His)6-Thrombin cleavage site fusion of human gene or portion of gene with point mutation in bacterial strains. pET based vector.

   Depositor

   Tara Davis, Melissa Jurica

   Article

   Davis et al PLoS Biol. 2010 Jul 27;8(7):e1000439. do

   Insert

   ppil2 (PPIL2 Human)
   

   Use

   Tags

   (His)6-thrombin

   Expression

   Bacterial

   Mutation

   280-457-Y389H

   Promoter

   Available since

   Aug. 4, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. ppil2.280.457.Y389W

   Plasmid

   #137657

   Purpose

   Expresses N-terminal (His)6-Thrombin cleavage site fusion of human gene or portion of gene with point mutation in bacterial strains. pET based vector.

   Depositor

   Tara Davis, Melissa Jurica

   Article

   Davis et al PLoS Biol. 2010 Jul 27;8(7):e1000439. do

   Insert

   ppil2 (PPIL2 Human)
   

   Use

   Tags

   (His)6-thrombin

   Expression

   Bacterial

   Mutation

   280-457-Y389W

   Promoter

   Available since

   Aug. 4, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. KTK_226

   Plasmid

   #180552

   Purpose

   Entry vector containing K.rhaeticus native promoter pRutB (200 bases upstream from gene start codon)

   Depositor

   Tom Ellis

   Article

   Goosens et al ACS Synth Biol. 2021 Dec 17;10(12):3422-

   Insert

   K.rhaeticus native promoter pRutB (200 bases upstream from gene start codon)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   June 8, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. Lenti-sgRnf43#2/Cre

    Plasmid

    #173614

    Purpose

    Expresses a Rnf43-targeting gRNA and Cre-recombinase

    Depositor

    Charles Swanton

    Article

    Cai et al Cancer Discov. 2021 Feb 19. pii: 2159-82

    Insert

    gRNA targeting Rnf43 (Rnf43 Mouse)
    

    Use

    CRISPR, Cre/Lox, and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. TKIT NFL gRNA1 gRNA2

    Plasmid

    #182681

    Purpose

    Expression of spCas9, gRNA targeting intron 3 and gRNA targeting 3'UTR of the mouse Nefl gene. Can be used for the tagging of endogenous NFL via Targeted Knock-In with Two guides (TKIT) approach.

    Depositor

    Ivana Nikić-Spiegel

    Article

    Arsic et al Nat Commun. 2022 Jan 14;13(1):314. doi:

    Insert

    two Nefl-targeting gRNAs, one targets intron 3 and the other 3'UTR of the Nefl gene (Nefl Mouse)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6 and chicken beta-actin promoter

    Available since

    May 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. KTK_218

    Plasmid

    #180533

    Purpose

    Entry vector containing K.rhaeticus native promoter pTtaC (200 bases upstream from gene start codon)

    Depositor

    Tom Ellis

    Article

    Goosens et al ACS Synth Biol. 2021 Dec 17;10(12):3422-

    Insert

    K.rhaeticus native promoter pTtaC (200 bases upstream from gene start codon)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. KTK_225

    Plasmid

    #180539

    Purpose

    Entry vector containing K.rhaeticus native promoter pBlh (200 bases upstream from gene start codon)

    Depositor

    Tom Ellis

    Article

    Goosens et al ACS Synth Biol. 2021 Dec 17;10(12):3422-

    Insert

    K.rhaeticus native promoter pBlh (200 bases upstream from gene start codon)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. KTK_221

    Plasmid

    #180536

    Purpose

    Entry vector containing K.rhaeticus native promoter pKr0953 (200 bases upstream from gene start codon)

    Depositor

    Tom Ellis

    Article

    Goosens et al ACS Synth Biol. 2021 Dec 17;10(12):3422-

    Insert

    K.rhaeticus native promoter pKr0953 (200 bases upstream from gene start codon)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. KTK_220

    Plasmid

    #180535

    Purpose

    Entry vector containing K.rhaeticus native promoter pAacsAB4 (200 bases upstream from gene start codon)

    Depositor

    Tom Ellis

    Article

    Goosens et al ACS Synth Biol. 2021 Dec 17;10(12):3422-

    Insert

    K.rhaeticus native promoter pAacsAB4 (200 bases upstream from gene start codon)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. KTK_222

    Plasmid

    #180537

    Purpose

    Entry vector containing K.rhaeticus native promoter pCyd (200 bases upstream from gene start codon)

    Depositor

    Tom Ellis

    Article

    Goosens et al ACS Synth Biol. 2021 Dec 17;10(12):3422-

    Insert

    K.rhaeticus native promoter pCyd (200 bases upstream from gene start codon)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. PX459_GRHL1-exon8

    Plasmid

    #177764

    Purpose

    sgRNA for CRISPR/Cas9-mediated deletion of human GRHL1

    Depositor

    Michael Ristow

    Article

    Grigolon et al Nat Commun. 2022 Jan 10;13(1):107. doi:

    Insert

    GRHL1 (GRHL1 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 5, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. eSpCas9(1.1)-ABL1-gRNA1-exon4

    Plasmid

    #163322

    Purpose

    eCas9 ABL1 gRNA 1 (GGGGGACACACCATAGACAG), targeting exon 4 within the protein kinase domain of both ABL1 isoforms 1a and 1b.

    Depositor

    Val Brunton

    Article

    Beetham et al Cancer Res. 2021 Dec 17. pii: 0008-5472.

    Insert

    ABL1 gRNA 1_Exon4
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Jan. 7, 2022

    Availability

    Academic Institutions and Nonprofits only

19. eSpCas9(1.1)-ABL1-gRNA2-exon4

    Plasmid

    #163323

    Purpose

    eCas9 ABL1 gRNA 2 (GAAGAAATACAGCCTGACGG), targeting exon 4 within the protein kinase domain of both ABL1 isoforms 1a and 1b.

    Depositor

    Val Brunton

    Article

    Beetham et al Cancer Res. 2021 Dec 17. pii: 0008-5472.

    Insert

    ABL1 gRNA 2_Exon4
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Jan. 7, 2022

    Availability

    Academic Institutions and Nonprofits only

20. pLV-EF1a-HaloSFPQY527A

    Plasmid

    #166949

    Purpose

    Lentiviral plasmid expressing Halo-tagged SFPQ Y527A protein from the EF1a promoter

    Depositor

    Rosalind Segal

    Article

    Fukuda et al J Cell Biol. 2021 Jan 4;220(1). pii: 211

    Insert

    SFPQ-Y527A (SFPQ Human)
    

    Use

    Lentiviral

    Tags

    Halo

    Expression

    Mutation

    SFPQ-Y527A

    Promoter

    eF1a

    Available since

    March 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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21. UbC-Halo-SFPQY527A

    Plasmid

    #166947

    Purpose

    Lentiviral plasmid expressing Halo-tagged SFPQ Y527A protein from the UbC promoter

    Depositor

    Rosalind Segal

    Article

    Fukuda et al J Cell Biol. 2021 Jan 4;220(1). pii: 211

    Insert

    SFPQ-Y527A (SFPQ Human)
    

    Use

    Lentiviral

    Tags

    Halo

    Expression

    Mutation

    SFPQ-Y527A

    Promoter

    UbC

    Available since

    March 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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22. pMKO.1-shCenpf

    Plasmid

    #160956

    Purpose

    Cenpf shRNA in pMKO.1 retroviral vector

    Depositor

    Matthew Steinhauser

    Article

    Zhang et al J Clin Invest. 2018 Nov 1;128(11):4898-4

    Insert

    Cenpf shRNA (Cenpf Mouse)
    

    Use

    RNAi and Retroviral

    Tags

    Expression

    Mutation

    Promoter

    U6

    Available since

    Dec. 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pX330_gRNA_H19-DMR-2

    Plasmid

    #159930

    Purpose

    Specific gRNA against mouse H19-DMR cloned in the pX330 backbone (Addgene Number 42230). Deletion of H19-DMR in mouse ESC.

    Depositor

    Anton Wutz

    Article

    Aizawa et al PLoS One. 2020 Sep 10;15(9):e0233072. do

    Insert

    gRNA mouse H19-DMR (H19-icr Mouse)
    

    Use

    CRISPR and Mouse Targeting

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6, CBh

    Available since

    Nov. 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. LB001: pMAGIC (L3-L2) 3x HA epitope tag + polyA; hU6::Sp/xCas9 TRE#2 gRNA

    Plasmid

    #131757

    Purpose

    pMAGIC L3-L2 entry plasmid, contains 3xHA tag+polyA; hU6-driven Sp/xCas9 TRE#2 gRNA for 3-/4-component MultiSite Gateway Pro assembly. Allows C-term HA epitope fusion to dCas9 w/ TRE#2 gRNA expression

    Depositor

    Christopher Newgard

    Article

    Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

    Insert

    TRE#2 Sp/xCas9 gRNA
    

    Use

    Synthetic Biology; Pmagic gateway entry plasmid

    Tags

    Expression

    Mutation

    Promoter

    hU6

    Available since

    Oct. 15, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. LA901: pMAGIC (L3-L2) 3x HA epitope tag + polyA; hU6::Sp/xCas9 TRE#1 gRNA

    Plasmid

    #131756

    Purpose

    pMAGIC L3-L2 entry plasmid, contains 3xHA tag+polyA; hU6-driven Sp/xCas9 TRE#1 gRNA for 3-/4-component MultiSite Gateway Pro assembly. Allows C-term HA epitope fusion to dCas9 w/ TRE#1 gRNA expression

    Depositor

    Christopher Newgard

    Article

    Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

    Insert

    TRE#1 Sp/xCas9 gRNA
    

    Use

    Synthetic Biology; Pmagic gateway entry plasmid

    Tags

    Expression

    Mutation

    Promoter

    hU6

    Available since

    Oct. 15, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. JV201: pMAGIC (L3-L2) 3x HA epitope tag + polyA; hU6::SaCas9 TRE#1 gRNA

    Plasmid

    #131755

    Purpose

    pMAGIC L3-L2 entry plasmid, contains 3xHA tag+polyA; hU6-driven SaCas9 TRE#1 gRNA for 3-/4-component MultiSite Gateway Pro assembly. Allows C-term HA epitope fusion to dCas9 w/ TRE#1 gRNA expression

    Depositor

    Christopher Newgard

    Article

    Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

    Insert

    TRE#1 SaCas9 gRNA
    

    Use

    Synthetic Biology; Pmagic gateway entry plasmid

    Tags

    Expression

    Mutation

    Promoter

    hU6

    Available since

    Oct. 15, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pAAV-hSyn-DIO-HA-CB2SH3(-)-IRES-mCitrine

    Plasmid

    #160069

    Purpose

    Conditionally overexpress collybistin (CB2SH3-) and mCitrine

    Depositor

    Angel de Blas

    Article

    George et al eNeuro. 2021 Jun 2. pii: ENEURO.0561-20.

    Insert

    ARHGEF9 (Arhgef9 Rat)
    

    Use

    AAV

    Tags

    HA- in CB2SH3

    Expression

    Mutation

    Promoter

    human Synapsin promoter

    Available since

    Oct. 5, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pLCHKO_SFRS7_exon_deletion_2

    Plasmid

    #155070

    Purpose

    Lentiviral plasmid for expressing U6 driven hybrid guide (hg)RNA for deletion of SFRS7 exon using SpCas9 and LbCas12a nucleases (CHyMErA system)

    Depositor

    Jason Moffat, Benjamin Blencowe

    Article

    Gonatopoulos-Pournatzis et al Nat Biotechnol. 2020 May;38(5):638-648.

    Insert

    (hg)RNA for deletion of SFRS7 exon using SpCas9 and LbCas12a nucleases
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Aug. 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pLCHKO_MDM4_exon_deletion_2

    Plasmid

    #155074

    Purpose

    Lentiviral plasmid for expressing U6 driven hybrid guide (hg)RNA for deletion of MDM4 exon using SpCas9 and LbCas12a nucleases (CHyMErA system)

    Depositor

    Jason Moffat, Benjamin Blencowe

    Article

    Gonatopoulos-Pournatzis et al Nat Biotechnol. 2020 May;38(5):638-648.

    Insert

    (hg)RNA for deletion of MDM4 exon using SpCas9 and LbCas12a nucleases
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Aug. 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pLCHKO_Ptbp_ex8_1

    Plasmid

    #155081

    Purpose

    Lentiviral plasmid for expressing U6 driven hybrid guide (hg)RNA for deletion of mouse Ptbp exon 8 using SpCas9 and LbCas12a nucleases (CHyMErA system)

    Depositor

    Jason Moffat, Benjamin Blencowe

    Article

    Gonatopoulos-Pournatzis et al Nat Biotechnol. 2020 May;38(5):638-648.

    Insert

    (hg)RNA for deletion of mouse Ptbp exon 8 using SpCas9 and LbCas12a nucleases
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Aug. 12, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge