We narrowed to 12,214 results for: LEA
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Plasmid#84635PurposeEncodes C-terminal (substrate) fragment of bimolecular AMPK/BRSK activity reporter (bimABKAR); cytosol targeted; use in conjunction with pcDNA3-Cerulean-FHA1-NESDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertAMPKsub-YPet-NES
Tags6xHis, Nuclear export signal (NES), T7 tag (gene …ExpressionMammalianPromoterCMVAvailable sinceApril 27, 2018AvailabilityAcademic Institutions and Nonprofits only -
pX330-Flag-eSpCas9 (without sgRNA)
Plasmid#92354PurposeExpression plasmid for human codon-optimized high-fidelity eSpCas9 (without U6-sgRNA coding sequence)DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsert3xFLAG-NLS-Streptococcus pyogenes enhanced Cas9-NLS
UseCRISPRTags3xFLAG and NLSExpressionMammalianMutationK848A, K1003A, R1060APromoterCbhAvailable sinceOct. 30, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAFMW-Nbr-D435A/E437A
Plasmid#40829PurposeExpresses Flag, Myc tagged mutant Nbr in Drosophila cellsDepositorInsertNbr D435A/E437A (Nbr Fly)
Tags3xFLAG and 6xMycExpressionInsectMutationD435A/E437A, part of the DEDD motif; mutations p…PromoterActin5CAvailable sinceSept. 4, 2013AvailabilityAcademic Institutions and Nonprofits only -
pPbuCas13b
Plasmid#184565PurposeInducible expression of PbuCas13b nuclease in bacteria.DepositorInsertCas13b nuclease from Prevotella buccae
ExpressionBacterialPromoterT7 promoterAvailable sinceNov. 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
SUMO_TwinStrep_Csx30
Plasmid#197611PurposeFor protein purification of Csx30DepositorInsertSUMO_TwinStrep_Csx30
ExpressionBacterialAvailable sinceMay 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-mCherry-Rheb(C181S)
Plasmid#192437PurposeEncodes mCherry-tagged, farnesylation-deficient Rheb C181S mutant.DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertmCherry-Rheb(C181S) (RHEB Human)
Tags6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…ExpressionMammalianMutationRheb Cys 181 mutated to Ser; abolishes C-terminal…PromoterCMVAvailable sinceDec. 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
pEF NS34a-msGFP-A'Q-mito
Plasmid#158763PurposeExpression of self-cleaving membrane targeting peptide containing the OMP25 C-terminal targeting sequence, a modified P6P4 NS5A/5B cleavage site, msGFP and the NS3/4A protease.DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertNS3/4a protease and msGFP
ExpressionMammalianMutationFlexible linkers between the protease and msGFP a…Available sinceFeb. 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-JNKAR1-NES
Plasmid#84632PurposeUnimolecular, FRET-based JNK activity reporter; cytosol targetedDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertECFP-FHA1-JNKsubDD-Citrine-NES
Tags6xHis, Citrine, ECFP, Nuclear export signal (NES)…ExpressionMammalianPromoterCMVAvailable sinceNov. 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
pGL3-Basic-hCYP26A1-FL- luciferase
Plasmid#135566PurposeFull Length promoter of the human CYP26A1 gene in pGL3-Basic-luciferaseDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertFull-length promoter of human retinoic acid receptor-beta gene
PromoterhHuman CYP26A1 gene promoterAvailable sinceJan. 9, 2020AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pcDNA3.1-LGC-53
Plasmid#86399PurposeMammalian expression of codon-optimized dopamine receptor LGC-53DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertLGC-53 (lgc-53 Nematode, Synthetic)
ExpressionMammalianMutationCodon optimized for mammalian expression; Signal …PromoterCMVAvailable sinceJan. 25, 2017AvailabilityAcademic Institutions and Nonprofits only -
pWay21-MKP-1 ASA
Plasmid#13479DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertMKP-1ASA (Dusp1 Mouse)
TagsEGFPExpressionMammalianMutationChanged Arginine 53 to Alanine, Changed Arginine…Available sinceDec. 8, 2006AvailabilityAcademic Institutions and Nonprofits only -
pET21b-LaG9(S151C)
Plasmid#172490PurposeEncodes the anti-GFP LaG-9 nanobody with a cysteine near the C-terminus, LaG-9(S151C).DepositorInsertLaG9(S151C)
Tags6xHis, PPX cleavage site, and pelB leader sequenc…ExpressionBacterialMutationS151CPromoterT7Available sinceMarch 7, 2022AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pET28a-TEV-U1AF37MF77M
Plasmid#168267PurposeExpresses human dmU1A with the F37M/F77M double mutantDepositorInsertU1A RRM1 crystallization module (SNRPA Human)
Tags6xHis tag N-terminus, TEV protease cleavage siteExpressionBacterialMutationchanged F37M and F77MPromoterT7 promotorAvailable sinceApril 16, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGBW-m4046854
Plasmid#145759PurposeBacterial Expression plasmid for SARS-CoV-2 leader proteinDepositorInsertSARS-CoV-2 leader protein (ORF1ab Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
TagsCleavable TEV;6xHISExpressionBacterialMutationEscherichia coli recode 1PromoterPromoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…Available sinceApril 29, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
Cas12j-8-crRNA
Plasmid#194967PurposeThe plasmid expresses Cas12j-8-crRNA expression elements.DepositorTypeEmpty backboneExpressionMammalianAvailable sinceFeb. 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
AAV-H2GH
Plasmid#182202PurposeAdeno-associated viral vector to express histone2B-HaloTag-GFP fusion, a nuclear-localized Halotag-GFP fusion constructDepositorInserthistone2B-HaloTag-GFP
UseAAVAvailable sinceMay 10, 2022AvailabilityAcademic Institutions and Nonprofits only -
B1-3
Plasmid#111465Purposetranslational release factor 3DepositorInserttranslational release factor 3
Tags6xHisExpressionBacterialAvailable sinceJan. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
p2CT-Cdi
Plasmid#83044PurposeExpresses Corynebacterium diphtheriae Cas9 (CdiCas9)DepositorInsertCdiCas9
TagsHis-MBPExpressionBacterialAvailable sinceNov. 1, 2016AvailabilityAcademic Institutions and Nonprofits only -
pOPINB-HOIL-1 full-length (1–510)
Plasmid#193858PurposeExpression of human His-tagged HOIL-1 (RBCK1) full-length (1–510) codon optimized for E. coliDepositorInsertHOIL-1 (RBCK1 Human)
Tags3C protease cleavage site and 6x-His tagExpressionBacterialMutationCodon optimised for expression in E. coliPromoterT7Available sinceJan. 12, 2023AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pGP-CMV-GCaMP3-373
Plasmid#91779PurposeMammalian expression of low affinity calcium-sensing fluorescent proteinDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertGCaMP3-373
Tags6xHis, T7 epitope, and Xpress tagExpressionMammalianPromoterCMVAvailable sinceJune 27, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by