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Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

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We narrowed to 5,897 results for: pcDNA 3.1

Showing: 5431 - 5460 of 5897 results

  1. L2117-FKBP-GPA-114-RH-CyOFP1

    Plasmid
    #222884
    Purpose
    Biosensor chain detecting rapamycin and responding with BRET. FKBP ectodomain, human Glycophorin A (GPA) scaffold, 20 GS linker, split Nanoluciferase fragment 114, RH linker, CyOFP1 protein.
    Depositor
    Joshua Leonard
    Article
    Dolberg et al ACS Synth Biol. 2024 Apr 19;13(4):1273-1
    Insert
    FKBP-GPA-20GS-NanoLuc114-RH-CyOFP1
    Use
    Tags
    Myc
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Sept. 9, 2024
    Availability
    Academic Institutions and Nonprofits only
    222884-plasmid-map-sequence-id-444499

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  2. L2116-FRB-GPA-114-RH-CyOFP1

    Plasmid
    #222883
    Purpose
    Biosensor chain detecting rapamycin and responding with BRET. FRB ectodomain, human Glycophorin A (GPA) scaffold, 20 GS linker, split Nanoluciferase fragment 114, RH linker, CyOFP1 protein.
    Depositor
    Joshua Leonard
    Article
    Dolberg et al ACS Synth Biol. 2024 Apr 19;13(4):1273-1
    Insert
    FRB-GPA-20GS-NanoLuc114-RH-CyOFP1
    Use
    Tags
    Myc
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Sept. 9, 2024
    Availability
    Academic Institutions and Nonprofits only
    222883-plasmid-map-sequence-id-444497

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  3. L2379-FKBP-GPA(V84R)-20-11S

    Plasmid
    #222901
    Purpose
    Biosensor chain detecting rapamycin and responding with split Nanoluciferase reconstitution. FKBP ectodomain, human Glycophorin A (GPA) (V84R) scaffold, 20 GS linker, split Nanoluciferase fragment 11S
    Depositor
    Joshua Leonard
    Article
    Dolberg et al ACS Synth Biol. 2024 Apr 19;13(4):1273-1
    Insert
    FKBP-GPA(V84R)-20GS-NanoLuc11S
    Use
    Tags
    Myc
    Expression
    Mammalian
    Mutation
    V84R in GPA
    Promoter
    CMV
    Available since
    Aug. 12, 2024
    Availability
    Academic Institutions and Nonprofits only
    222901-plasmid-map-sequence-id-441651

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  4. L2380-FKBP-GPA(V84R)-20-114

    Plasmid
    #222900
    Purpose
    Biosensor chain detecting rapamycin and responding with split Nanoluciferase reconstitution. FKBP ectodomain, human Glycophorin A (GPA) (V84R) scaffold, 20 GS linker, split Nanoluciferase fragment 114
    Depositor
    Joshua Leonard
    Article
    Dolberg et al ACS Synth Biol. 2024 Apr 19;13(4):1273-1
    Insert
    FKBP-GPA(V84R)-20GS-NanoLuc114
    Use
    Tags
    Myc
    Expression
    Mammalian
    Mutation
    V84R in GPA
    Promoter
    CMV
    Available since
    Aug. 12, 2024
    Availability
    Academic Institutions and Nonprofits only
    222900-plasmid-map-sequence-id-441786

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  5. L2378-FRB-GPA(V84R)-20-11S

    Plasmid
    #222899
    Purpose
    Biosensor chain detecting rapamycin and responding with split Nanoluciferase reconstitution. FRB ectodomain, human Glycophorin A (GPA) (V84R) scaffold, 20 GS linker, split Nanoluciferase fragment 11S.
    Depositor
    Joshua Leonard
    Article
    Dolberg et al ACS Synth Biol. 2024 Apr 19;13(4):1273-1
    Insert
    FRB-GPA(V84R)-20GS-NanoLuc11S
    Use
    Tags
    Myc
    Expression
    Mammalian
    Mutation
    V84R in GPA
    Promoter
    CMV
    Available since
    Aug. 12, 2024
    Availability
    Academic Institutions and Nonprofits only
    222899-plasmid-map-sequence-id-440196

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  6. L2377-FRB-GPA(V84R)-20-114

    Plasmid
    #222898
    Purpose
    Biosensor chain detecting rapamycin and responding with split Nanoluciferase reconstitution. FRB ectodomain, human Glycophorin A (GPA) (V84R) scaffold, 20 GS linker, split Nanoluciferase fragment 114.
    Depositor
    Joshua Leonard
    Article
    Dolberg et al ACS Synth Biol. 2024 Apr 19;13(4):1273-1
    Insert
    FRB-GPA(V84R)-20GS-NanoLuc114
    Use
    Tags
    Myc
    Expression
    Mammalian
    Mutation
    V84R in GPA
    Promoter
    CMV
    Available since
    Aug. 12, 2024
    Availability
    Academic Institutions and Nonprofits only
    222898-plasmid-map-sequence-id-440197

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  7. pTFG011_Spike_Parental_D614G_3xFlag

    Plasmid
    #191571
    Purpose
    SARS-CoV-2 Spike protein under CMV with D614G, C-term 19 amino acid truncation, and 3x FLAG. For expression and pseudotyping lentiviral particles.
    Depositor
    Joshua Leonard
    Article
    Gunnels et al Small. 2022 May;18(19):e2200125. doi: 10
    Insert
    SARS-CoV-2 Spike Protein (S Synthetic)
    Use
    Tags
    3x FLAG
    Expression
    Mammalian
    Mutation
    D614G mutation. Last 19 amino acids truncated.
    Promoter
    CMV
    Available since
    July 25, 2024
    Availability
    Academic Institutions and Nonprofits only
    191571-plasmid-map-sequence-id-440477

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  8. L2132-FKBP-GPA-114-5-CyOFP1

    Plasmid
    #222891
    Purpose
    Biosensor chain detecting rapamycin and responding with BRET. FKBP ectodomain, human Glycophorin A (GPA) scaffold, 20 GS linker, split Nanoluciferase fragment 114, 5 GS linker, CyOFP1 protein.
    Depositor
    Joshua Leonard
    Article
    Dolberg et al ACS Synth Biol. 2024 Apr 19;13(4):1273-1
    Insert
    FKBP-GPA-20GS-NanoLuc114-5GS-CyOFP1
    Use
    Tags
    Myc
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    July 24, 2024
    Availability
    Academic Institutions and Nonprofits only
    222891-plasmid-map-sequence-id-440208

    Enlarge

  9. L2127-FRB-GPA-114-20-CyOFP1

    Plasmid
    #222886
    Purpose
    Biosensor chain detecting rapamycin and responding with BRET. FRB ectodomain, human Glycophorin A (GPA) scaffold, 20 GS linker, split Nanoluciferase fragment 114, 20 GS linker, CyOFP1 protein.
    Depositor
    Joshua Leonard
    Article
    Dolberg et al ACS Synth Biol. 2024 Apr 19;13(4):1273-1
    Insert
    FRB-GPA-20GS-NanoLuc114-20GS-CyOFP1
    Use
    Tags
    Myc
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    July 24, 2024
    Availability
    Academic Institutions and Nonprofits only
    222886-plasmid-map-sequence-id-440205

    Enlarge

  10. L2129-FRB-GPA-114-5-CyOFP1

    Plasmid
    #222888
    Purpose
    Biosensor chain detecting rapamycin and responding with BRET. FRB ectodomain, human Glycophorin A (GPA) scaffold, 20 GS linker, split Nanoluciferase fragment 114, 5 GS linker, CyOFP1 protein.
    Depositor
    Joshua Leonard
    Article
    Dolberg et al ACS Synth Biol. 2024 Apr 19;13(4):1273-1
    Insert
    FRB-GPA-20GS-NanoLuc114-5GS-CyOFP1
    Use
    Tags
    Myc
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    July 24, 2024
    Availability
    Academic Institutions and Nonprofits only
    222888-plasmid-map-sequence-id-440203

    Enlarge

  11. L2128-FRB-GPA-114-10-CyOFP1

    Plasmid
    #222887
    Purpose
    Biosensor chain detecting rapamycin and responding with BRET. FRB ectodomain, human Glycophorin A (GPA) scaffold, 20 GS linker, split Nanoluciferase fragment 114, 10 GS linker, CyOFP1 protein.
    Depositor
    Joshua Leonard
    Article
    Dolberg et al ACS Synth Biol. 2024 Apr 19;13(4):1273-1
    Insert
    FRB-GPA-20GS-NanoLuc114-10GS-CyOFP1
    Use
    Tags
    Myc
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    July 24, 2024
    Availability
    Academic Institutions and Nonprofits only
    222887-plasmid-map-sequence-id-440200

    Enlarge

  12. L2131-FKBP-GPA-114-10-CyOFP1

    Plasmid
    #222890
    Purpose
    Biosensor chain detecting rapamycin and responding with BRET. FKBP ectodomain, human Glycophorin A (GPA) scaffold, 20 GS linker, split Nanoluciferase fragment 114, 10 GS linker, CyOFP1 protein.
    Depositor
    Joshua Leonard
    Article
    Dolberg et al ACS Synth Biol. 2024 Apr 19;13(4):1273-1
    Insert
    FKBP-GPA-20GS-NanoLuc114-10GS-CyOFP1
    Use
    Tags
    Myc
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    July 24, 2024
    Availability
    Academic Institutions and Nonprofits only
    222890-plasmid-map-sequence-id-440190

    Enlarge

  13. L2130-FKBP-GPA-114-20-CyOFP1

    Plasmid
    #222889
    Purpose
    Biosensor chain detecting rapamycin and responding with BRET. FKBP ectodomain, human Glycophorin A (GPA) scaffold, 20 GS linker, split Nanoluciferase fragment 114, 20 GS linker, CyOFP1 protein.
    Depositor
    Joshua Leonard
    Article
    Dolberg et al ACS Synth Biol. 2024 Apr 19;13(4):1273-1
    Insert
    FKBP-GPA-20GS-NanoLuc114-20GS-CyOFP1
    Use
    Tags
    Myc
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    July 24, 2024
    Availability
    Academic Institutions and Nonprofits only
    222889-plasmid-map-sequence-id-440187

    Enlarge

  25. pTS2308-Tier1-pGL3-PPGK-NLuc_TB

    Plasmid
    #169551
    Purpose
    Tier-1 expression vector encoding PmPGK1-driven secreted Nluc coupled to fluorescent reporter mTagBFP2 that is insulated via a 5' insulator sequence derived from the pGL3 plasmid (pGL3-PmPGK1-IgkSS-Nluc-p2A-mTagBFP2-pA).
    Depositor
    Martin Fussenegger
    Article
    Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10
    Insert
    pGL3-insulator-modulated PPGK-driven NanoLuc Luciferase - mTagBFP expression cassette
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    PPGK
    Available since
    June 22, 2022
    Availability
    Academic Institutions and Nonprofits only
    169551-plasmid-map-sequence-id-364981

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  26. pRD400

    Plasmid
    #163107
    Purpose
    Expression of eYFP_H-NSdbd in eukaryotes for visualisation of nuclear DNA
    Depositor
    Remus Dame
    Article
    Rashid et al Nucleic Acids Res. 2021 Nov 4. pii: 6414
    Insert
    NLS_eYFP_H-NSdbd_NLS
    Use
    Eukaryotic expression
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV promoter; T7 promoter
    Available since
    Oct. 29, 2021
    Availability
    Academic Institutions and Nonprofits only
    163107-plasmid-map-sequence-id-321848

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  27. pRD188

    Plasmid
    #163099
    Purpose
    Expression of mEos3.2_H-NSdbd in eukaryotes for visualisation of nuclear DNA
    Depositor
    Remus Dame
    Article
    Rashid et al Nucleic Acids Res. 2021 Nov 4. pii: 6414
    Insert
    NLS_mEos3.2_H-NSdbd_NLS
    Use
    Eukaryotic expression
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV promoter; T7 promoter
    Available since
    Oct. 29, 2021
    Availability
    Academic Institutions and Nonprofits only
    163099-plasmid-map-sequence-id-321840

    Enlarge

  28. S113

    Plasmid
    #174551
    Purpose
    Constitutive PhCMV-driven mammalian expression vector of CDH-3-TF cassette (PhCMV-Gal4-mdm2-P2A-LD6-Rel65-pA)
    Depositor
    Bruno Correia
    Article
    Shui et al Nat Commun. 2021 Oct 1;12(1):5754. doi:
    Insert
    mdm2 and designed protein LD6 (MDM2 )
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Oct. 1, 2021
    Availability
    Academic Institutions and Nonprofits only
    174551-plasmid-map-sequence-id-343593

    Enlarge

  30. 1010-AIDx-CP

    Plasmid
    #135340
    Purpose
    Expression of circular permutant of nSpyCas9 at amino acid position 1010 encoding truncated human cytosine deaminase, hAIDx, at the C-terminus.
    Depositor
    Alex Hewitt
    Article
    Nguyen Tran et al Nat Commun. 2020 Sep 25;11(1):4871. doi:
    Insert
    CP 1010 nSpyCas9 -hAIDx
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    WT
    Promoter
    Available since
    Oct. 1, 2020
    Availability
    Academic Institutions and Nonprofits only
    135340-plasmid-map-sequence-id-267691

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Showing: 5431 - 5460 of 5897 results