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pDuet TDP-43 M337V

Plasmid

#27464

Depositor

Aaron Gitler

Article

Johnson et al J Biol Chem. 2009 Jul 24. 284(30):20329-

Insert

TDP-43 (TARDBP Human)

Use

Coexpression

Expression

Bacterial

Mutation

Methionine 337 mutated to Valine (M337V)

Available Since

Feb. 10, 2011

Availability

Academic Institutions and Nonprofits only
pEF-DEST51-GOLPH3

Plasmid

#21690

Depositor

Lynda Chin

Article

Scott et al Nature. 2009 Jun 25. 459(7250):1085-90.

Insert

Golgi Phosphoprotein 3 (GOLPH3 Human)

Use

Invitrogen gateway destination vector

Tags

6xHIS and V5

Expression

Mammalian

Available Since

July 28, 2009

Availability

Academic Institutions and Nonprofits only

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pGEX-KG-CAND1

Plasmid

#19949

Depositor

Yue Xiong

Article

Liu et al Mol Cell. 2002 Dec . 10(6):1511-8.

Insert

CAND1 (cullin-associated and neddylation-dissociated 1) (CAND1 Human)

Tags

GST

Expression

Bacterial

Promoter

tac

Available Since

April 28, 2009

Availability

Academic Institutions and Nonprofits only
pGL4 mIL-17 0.6kb promoter

Plasmid

#20126

Depositor

Warren Strober

Article

Fuping et al 2008 Nov;9(11):1297-306. Epub 2008 Oct 1

Insert

mIL-17 (Il17a Mouse)

Use

Luciferase

Available Since

Jan. 16, 2009

Availability

Academic Institutions and Nonprofits only
pAC-SP6

Plasmid

#102418

Purpose

Ac transposase with SP6 promoter for in vitro transcription

Depositor

Tatjana Sauka-Spengler

Article

Chong-Morrison et al Biol Open. 2023 Jun 15;12(6):bio059995.

Insert

Ac transposase

Expression

Bacterial

Promoter

SP6

Available Since

Oct. 30, 2017

Availability

Academic Institutions and Nonprofits only

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Tet1-dCas9

Plasmid

#136650

Purpose

Tet1 fused to N-terminus of dCas9; pCDNA3 vector backbone, mammalian expression

Depositor

David Segal

Article

Umeh-Garcia et al Br J Cancer. 2022 Apr 19. pii: 10.1038/s

Insert

Tet1 (Tet1 Mouse)

Use

CRISPR

Tags

3XFLag-NLS-Tet1-dCas9-NLS

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 19, 2021

Availability

Academic Institutions and Nonprofits only

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HA-HA-BI-AR

Plasmid

#171234

Purpose

Mammalian expression of 2xHA-tagged human androgen receptor (hAR)

Depositor

Steven Balk

Article

Liu et al Oncotarget. 2016 Jan 12;7(2):1754-64. do

Insert

2xHA-tagged human androgen receptor (AR Human)

Tags

HA

Expression

Bacterial and Mammalian

Promoter

CMV

Available Since

June 25, 2021

Availability

Academic Institutions and Nonprofits only

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lentiCRISPR v2-sgSLC7A11/xCT-1

Plasmid

#161818

Purpose

knock out SLC7A11/xCT in mammalian cells

Depositor

Boyi Gan

Article

Liu et al Nat Cell Biol. 2020 Apr;22(4):476-486. d

Insert

SLC7A11 solute carrier family 7 member 11 xCT (SLC7A11 Human)

Use

CRISPR and Lentiviral

Available Since

Dec. 8, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+) Drp1 V2

Plasmid

#44599

Depositor

David Chan

Article

Loson et al Mol Biol Cell. 2013 Jan 2.

Insert

Drp1 variant 2 (Dnm1l Mouse)

Expression

Mammalian

Available Since

April 24, 2013

Availability

Academic Institutions and Nonprofits only

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pCDNA 3.1 Flag-rat-PTP1B

Plasmid

#35988

Depositor

Anna Huttenlocher

Article

Cortesio et al J Cell Biol. 2008 Mar 10;180(5):957-71.

Insert

protein tyrosine phosphatase 1B (Ptpn1 Rat)

Tags

Flag

Expression

Mammalian

Promoter

CMV

Available Since

June 1, 2012

Availability

Academic Institutions and Nonprofits only

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pMIR-CDH1 wtUTR

Plasmid

#25038

Depositor

Bob Weinberg

Article

Ma et al Nat Cell Biol. 2010 Mar . 12(3):247-56.

Insert

CDH1 3'UTR (CDH1 Human)

Use

Luciferase

Expression

Mammalian

Available Since

June 7, 2010

Availability

Academic Institutions and Nonprofits only
hBax S184E C3-EGFP

Plasmid

#19743

Depositor

Richard Youle

Article

Nechushtan et al EMBO J. 1999 May 4. 18(9):2330-41.

Insert

Bax S184E (BAX Human)

Tags

GFP

Expression

Mammalian

Mutation

changed Serine 184 to Glutamate

Available Since

Nov. 10, 2008

Availability

Academic Institutions and Nonprofits only
hEF1α-H2B-mVenus-IRES-mCherry PGK-Puromycin

Plasmid

#99278

Purpose

Dual fluorescent reporter - constitutive expression of mVenus (515/528) localized in the nucleus, mCherry (587/610) untargeted and visualized in the cytoplasm, and Puromycin resistance

Depositor

Jordan Miller

Article

Calderon et al Biomater Sci. 2017 Jun 29. doi: 10.1039/

Inserts

H2B-mVenus-IRES-mCherry
Puro

Use

Lentiviral

Tags

H2B, IRES, PGK, mCherry, and mVenus

Promoter

EF1 alpha-human and PGK-mouse

Available Since

Nov. 13, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-dCas9-dMQ1-EGFP

Plasmid

#89637

Purpose

Targeted CpG methylation

Depositor

Margaret Goodell

Article

Lei et al Nat Commun. 2017 Jul 11;8:16026. doi: 10

Insert

site-specific DNA-methyltransferase SssI

Use

CRISPR

Tags

3*FLAG, 6*His, and T2A-EGFP

Expression

Mammalian

Mutation

C141S, TGC-->TCC; S317A, AGC-->GCC

Promoter

CMV

Available Since

July 12, 2017

Availability

Academic Institutions and Nonprofits only

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pttq18

Plasmid

#69122

Purpose

regulated expression of genes in Escherichia coli

Depositor

Michael Stark

Article

Stark et al Gene. 1987;51(2-3):255-67.

Type

Empty backbone

Expression

Bacterial

Promoter

tac

Available Since

Oct. 9, 2015

Availability

Academic Institutions and Nonprofits only

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pFLEX+mCyclin D1

Plasmid

#86793

Purpose

mammalian expression of cyclin D1

Depositor

Martine Roussel, Charles Sherr

Article

Matsushime et al Cell. 1991 May 17;65(4):701-13.

Insert

cyclin D1 (Ccnd1 Mouse)

Tags

FLAG

Expression

Mammalian

Available Since

Feb. 14, 2017

Availability

Academic Institutions and Nonprofits only

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pFLAG-ING2

Plasmid

#13295

Depositor

Curtis Harris

Article

Pedeux et al Mol Cell Biol. 2005 Aug . 25(15):6639-48

Insert

ING2 (INGX Human)

Tags

Flag

Expression

Mammalian

Available Since

Oct. 16, 2006

Availability

Academic Institutions and Nonprofits only
pcDNA3-nuclear-EKAR4

Plasmid

#174439

Purpose

Nuclear-targeted EKAR4.

Depositor

Jin Zhang

Article

Keyes et al Elife. 2020 May 26;9. pii: 57410. doi: 1

Insert

nuclear-EKAR4

Tags

6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 1, 2021

Availability

Academic Institutions and Nonprofits only

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pflareG-psd95-exon18

Plasmid

#90266

Purpose

Minigene reporter for alternative splicing analysis

Depositor

Douglas Black

Article

Zheng et al Genome Res. 2013 Jun;23(6):998-1007. doi

Insert

Psd95-exon18 (Dlg4 Mouse)

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 26, 2017

Availability

Academic Institutions and Nonprofits only

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TPST2-EGFP

Plasmid

#66618

Purpose

Encodes GFP fusion of TPST2 trans Golgi marker (Tyrosyl protein sulfotransferase 2)

Depositor

David Stephens

Article

Spooner et al Biochem J. 2008 Sep 15;414(3):471-84. do

Insert

TPST2 (TPST2 Human)

Expression

Mammalian

Mutation

Insert contains T370A relative to NM_001008566.1

Available Since

July 28, 2015

Availability

Academic Institutions and Nonprofits only