We narrowed to 6,546 results for: proc

1. TccCas13a Twin SUMO

   Plasmid

   #199754

   Purpose

   Expresses codon optimized thermostabile TccCas13a in E.coli

   Depositor

   Magdy Mahfouz

   Article

   Mahas et al Proc Natl Acad Sci U S A. 2022 Jul 12;11

   Insert

   TccCas13a
   

   Use

   Tags

   SUMO

   Expression

   Bacterial

   Mutation

   Promoter

   LacI

   Available since

   June 29, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pET32a-HD16Q

   Plasmid

   #11487

   Depositor

   Pamela Bjorkman

   Article

   Bennett et al Proc Natl Acad Sci U S A. 2002 Sep 3. 99

   Insert

   huntingtin, exon 1, 16 glutamines (HTT Human)
   

   Use

   Tags

   His, His (out of frame), and Trx

   Expression

   Bacterial

   Mutation

   huntingtin exon 1 with 16 glutamines, vector m…

   Promoter

   Available since

   April 28, 2006

   Availability

   Academic Institutions and Nonprofits only

3. Tol2-lyzC-mcherry-2a-CDK2

   Plasmid

   #121131

   Purpose

   neutrophil specific expression

   Depositor

   Qing Deng

   Article

   Hsu et al Proc Natl Acad Sci U S A. 2019 Aug 26. p

   Insert

   dre-WT CDK2 (cdk2 Zebrafish)
   

   Use

   Tags

   mcherry-2a

   Expression

   Bacterial

   Mutation

   Promoter

   neutrophil specific lyzC

   Available since

   Sept. 9, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pSEVA228-pro4IUPi

   Plasmid

   #122018

   Purpose

   Expresses IUP pathway genes (ChK, IPK, idi) under constitutive promoter. Kan. resist.

   Depositor

   Gregory Stephanopoulos

   Article

   Chatzivasileiou et al Proc Natl Acad Sci U S A. 2018 Dec 24. p

   Inserts

   Choline kinase (CKI1 Budding Yeast)
   Isopentenyl monophosphate kinase (AT1G26640 Mustard Weed)
   isopentenyl-diphosphate Delta-isomerase
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Codon optimized for E. coli

   Promoter

   pro4

   Available since

   Oct. 16, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. ER-mGreenLantern

   Plasmid

   #164463

   Purpose

   Localization and retention of mGreenLantern fluorescent protein in the ER

   Depositor

   Gregory Petsko

   Article

   Campbell et al Proc Natl Acad Sci U S A. 2020 Dec 1;117

   Insert

   mGreenLantern
   

   Use

   Tags

   Calreticulin and KDEL

   Expression

   Mammalian

   Mutation

   Clover-F64L/S72A/E124V/N149K/I167T/S175G/A206K/L2…

   Promoter

   CMV

   Available since

   Jan. 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. LIC1B_Caspase-LOVN7C-7

   Plasmid

   #104629

   Purpose

   bacterial expression of light-activated caspase-3 with N7C-7 linker variation

   Depositor

   Jim Wells

   Article

   Smart et al Proc Natl Acad Sci U S A. 2017 Sep 26;11

   Insert

   Caspase-3 (CASP3 Human)
   

   Use

   Tags

   His and LOV2

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Aug. 29, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. AAV-PB-SRT-tdTomato

   Plasmid

   #154889

   Purpose

   piggyBac self-reporting transposon with tdTomato marker in AAV backbone

   Depositor

   Joseph Dougherty, Rob Mitra

   Article

   Cammack et al Proc Natl Acad Sci U S A. 2020 May 5;117

   Insert

   piggyBac SRT
   

   Use

   Adenoviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 1, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pFRLuc-CoV2-WT

   Plasmid

   #177614

   Purpose

   Dual luciferase reporter for SARS-CoV-2 programmed -1 ribosomal frameshifting

   Depositor

   Junjie Guo

   Article

   Sun et al Proc Natl Acad Sci U S A. 2021 Jun 29;11

   Insert

   SARS-CoV-2 FSE
   

   Use

   Luciferase

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 10, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pTwist-SFFV-NFE2L18ND-Puro

   Plasmid

   #181918

   Purpose

   Lentiviral plasmid that directs the expression of mutant NFE2L1/Nrf1 where 8 putative N-glycosites are mutated from asparagine to aspartic acid in mammalian cells.

   Depositor

   Scott Dixon

   Article

   Forcina et al Proc Natl Acad Sci U S A. 2022 Mar 15;11

   Insert

   NFE2L1 (NFE2L1 Human)
   

   Use

   Lentiviral

   Tags

   FLAG and HA

   Expression

   Mammalian

   Mutation

   8 putative N-glycoyslation sites converted from a…

   Promoter

   SFFV

   Available since

   April 6, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pcDNA3-GFP-LpIA(wild-type)

    Plasmid

    #61821

    Purpose

    Encodes a wild-type sequence of LplA and serves as the negative control for resorufin labeling

    Depositor

    Alice Ting

    Article

    Liu et al Proc Natl Acad Sci U S A. 2014 Oct 28;11

    Insert

    E. coli lipoic acid ligase
    

    Use

    Tags

    6XHis, EGFP, and Flag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 25, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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11. PGC-1 alpha promoter luciferase delta MEF

    Plasmid

    #8889

    Depositor

    Bruce Spiegelman

    Article

    Handschin et al Proc Natl Acad Sci U S A. 2003 Jun 10. 1

    Insert

    PGC-1 alpha promoter dMEF (Ppargc1a Mouse)
    

    Use

    Luciferase

    Tags

    luciferase

    Expression

    Mammalian

    Mutation

    Site-directed mutagenesis to remove the MEF2 bind…

    Promoter

    Available since

    Feb. 13, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCherryGFP-Inframe-noFSE

    Plasmid

    #177619

    Purpose

    Dual fluorescent protein reporter for SARS-CoV-2 programmed -1 ribosomal frameshifting. No FSE was inserted. mCherry and GFP are expressed equally (positive control).

    Depositor

    Junjie Guo

    Article

    Sun et al Proc Natl Acad Sci U S A. 2021 Jun 29;11

    Insert

    None
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pmCherry-Synaptojanin-1-145

    Plasmid

    #223563

    Purpose

    Expresses the short isoform of SYNJ1 (neuronal) with mCherry tagged at the n-terminus

    Depositor

    Pietro De Camilli

    Article

    Mohd Rafiq et al Proc Natl Acad Sci U S A. 2024 Apr 23;12

    Insert

    SYNJ1 (SYNJ1 Human)
    

    Use

    Tags

    His and mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pAAV-FLEX-hGTB

    Plasmid

    #26196

    Depositor

    Edward Callaway

    Article

    Wall et al Proc Natl Acad Sci U S A. 2010 Dec 14;10

    Insert

    Tet transactivator, enhanced green fluorescent protein, TVA, rabies B19 glycoprotein
    

    Use

    AAV and Cre/Lox; Adeno-associated virus

    Tags

    Expression

    Mutation

    F2A has 2A cleavage site deleted, producing htTA-…

    Promoter

    Available since

    Nov. 29, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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15. pGL3 basic Mut E2

    Plasmid

    #48748

    Purpose

    Expression of luciferase driven by mPer2 promoter fragment containing mutated E-box2 (bases -112 to +98 with respect to transcription start site at +1)

    Depositor

    Joseph Takahashi

    Article

    Yoo et al Proc Natl Acad Sci U S A. 2005 Feb 15;10

    Insert

    mPer2 promoter/enhancer region containing mutated E-box2 (-112 to +98)
    

    Use

    Luciferase

    Tags

    Expression

    Mutation

    Mutated E-box2 sequence from CACGTT to GCTAGT

    Promoter

    Available since

    Dec. 5, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCEP4-myc-HLA-A3

    Plasmid

    #135505

    Purpose

    Mammalian expression of myc-tagged HLA-A*03:01

    Depositor

    Erik Procko

    Article

    McShan et al Proc Natl Acad Sci U S A. 2019 Dec 17;11

    Insert

    HLA-A*03:01 (HLA-A Human)
    

    Use

    Tags

    Extracellular c-myc epitope tag and Signal peptid…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pcDNA3-myc-his-BACH1 K52R

    Plasmid

    #17643

    Depositor

    Ronny Drapkin

    Article

    Cantor et al Proc Natl Acad Sci U S A. 2004 Feb 24. 1

    Insert

    BACH1 (BRIP1 Human)
    

    Use

    Tags

    His and Myc

    Expression

    Mammalian

    Mutation

    The lysine residue at position 52 was changed to …

    Promoter

    Available since

    April 4, 2008

    Availability

    Academic Institutions and Nonprofits only

18. pSEVA331Bb

    Plasmid

    #78269

    Purpose

    pSEVA331 with Biobrick multiple cloning site

    Depositor

    Tom Ellis

    Article

    Florea et al Proc Natl Acad Sci U S A. 2016 Jun 14;11

    Type

    Empty backbone

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 10, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pTRE3G-GSX2

    Plasmid

    #96964

    Purpose

    Dox-inducible expression of Gsx2 in mammalian cells

    Depositor

    Elena Cattaneo

    Article

    Faedo et al Proc Natl Acad Sci U S A. 2017 Feb 14;11

    Insert

    GSX2 (Gsx2 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 31, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. pShuttle-Flag-XBP1u

    Plasmid

    #63679

    Purpose

    Adenoviral shuttle vector for XBP1u expression

    Depositor

    Qingbo Xu, Lingfang Zeng

    Article

    Zeng et al Proc Natl Acad Sci U S A. 2009 May 19;10

    Insert

    XBP1 (XBP1 Human)
    

    Use

    Adenoviral

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    unspliced

    Promoter

    CMV

    Available since

    March 23, 2015

    Availability

    Academic Institutions and Nonprofits only