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Showing: 7281 - 7320 of 25958 results

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pRSII42K-TDH3pr-mNeon

Plasmid

#194535

Purpose

High copy vector backbone with G418 selectivity, encodes for TDH3(pr) driven expression of mNeon

Depositor

Patrick Gibney

Article

Smith et al Appl Microbiol. 2024; 4(1):453-469. doi:

Insert

kanMX

Use

Tags

Expression

Yeast

Mutation

Promoter

TDH3

Available since

Oct. 31, 2024

Availability

Academic Institutions and Nonprofits only

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pRSII41N-TDH3pr-mNeon

Plasmid

#194536

Purpose

Low copy vector backbone with nourseothricin selectivity, encodes for TDH3(pr) driven expression of mNeon

Depositor

Patrick Gibney

Article

Smith et al Appl Microbiol. 2024; 4(1):453-469. doi:

Insert

natAC

Use

Tags

Expression

Yeast

Mutation

Promoter

TDH3

Available since

Oct. 31, 2024

Availability

Academic Institutions and Nonprofits only

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pRSII42N-TDH3pr-mNeon

Plasmid

#194537

Purpose

High copy vector backbone with nourseothricin selectivity, encodes for TDH3(pr) driven expression of mNeon

Depositor

Patrick Gibney

Article

Smith et al Appl Microbiol. 2024; 4(1):453-469. doi:

Insert

natAC

Use

Tags

Expression

Yeast

Mutation

Promoter

TDH3

Available since

Oct. 31, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pRSII41H-TDH3pr-mNeon

Plasmid

#194538

Purpose

Low copy vector backbone with hygromycin B selectivity, encodes for TDH3(pr) driven expression of mNeon

Depositor

Patrick Gibney

Article

Smith et al Appl Microbiol. 2024; 4(1):453-469. doi:

Insert

hphMX

Use

Tags

Expression

Yeast

Mutation

Promoter

TDH3

Available since

Oct. 31, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pRSII42H-TDH3pr-mNeon

Plasmid

#194539

Purpose

High copy vector backbone with hygromycin B selectivity, encodes for TDH3(pr) driven expression of mNeon

Depositor

Patrick Gibney

Article

Smith et al Appl Microbiol. 2024; 4(1):453-469. doi:

Insert

hphMX

Use

Tags

Expression

Yeast

Mutation

Promoter

TDH3

Available since

Oct. 31, 2024

Availability

Academic Institutions and Nonprofits only

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pTBL3401 BtoApegRNA-sfGFP

Plasmid

#226667

Purpose

PiggyBac cargo vector encoding B to A pegRNA marked with sfGFP

Depositor

Chang Liu

Article

Loveless et al bioRxiv 2021.11.05.467507

Insert

BtoA pegRNA

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

Oct. 28, 2024

Availability

Academic Institutions and Nonprofits only

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pAMC070n1a12

Plasmid

#209746

Purpose

ColA BBa_B0014 tetAp SD8 ScTrpRS rrnB1 proB SD8 Lum1PylRS BBaB10014RNAItrpA+ J23100 SD8 MmPylRS T3Te-T7Te DHFR

Depositor

Ahmed Badran

Article

Costello et al Nat Biotechnol. 2024 Sep 11. doi: 10.103

Insert

tRNA operon - ScTrpRS - Lum1PylRS - MmPylRS

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 18, 2024

Availability

Academic Institutions and Nonprofits only

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POSV801-SP44-SnaABCDEOinT1T2

Plasmid

#225058

Purpose

Heterologous expression in Streptomyces with inactive SnaO

Depositor

Wilfred van der Donk

Article

Yu et al ACS Cent Sci. 2024 Jun 3;10(6):1242-1250

Insert

inactivated SnaO

Use

Tags

Expression

Bacterial

Mutation

deltaR51-V511

Promoter

SP44

Available since

Oct. 16, 2024

Availability

Academic Institutions and Nonprofits only

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POSV801-SP44-SnaABCDEOT1T2

Plasmid

#225055

Purpose

Heterologous expression in Streptomyces for threopeptin biosynthetic gene cluster

Depositor

Wilfred van der Donk

Article

Yu et al ACS Cent Sci. 2024 Jun 3;10(6):1242-1250

Insert

SnaA

Use

Tags

Expression

Bacterial

Mutation

WT

Promoter

SP44

Available since

Oct. 16, 2024

Availability

Academic Institutions and Nonprofits only

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pLP4

Plasmid

#209959

Purpose

Entry vector to store Level 0* parts

Depositor

Thorsten Mascher

Article

Li et al ACS Synth Biol. 2024 Sep 20;13(9):2764-2

Type

Empty backbone

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 13, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pLP5

Plasmid

#209960

Purpose

Entry vector to store Level 0* resistance cassette parts

Depositor

Thorsten Mascher

Article

Li et al ACS Synth Biol. 2024 Sep 20;13(9):2764-2

Type

Empty backbone

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 13, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
Ub-LPNTG-pET

Plasmid

#221850

Purpose

Expresses Ub with sortase motif and His tag

Depositor

Artur Krężel

Article

Antonenko et al ACS Omega. 2024 Oct 30;9(45):45127-45137

Insert

Ubiquitin

Use

Tags

Expression

Bacterial

Mutation

Arg74ProLeuThr

Promoter

Available since

Aug. 29, 2024

Availability

Academic Institutions and Nonprofits only

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pLPC-C-SF-TAP-DEST-TLK1- M566T

Plasmid

#218831

Purpose

Expresses C-terminal Strep-FLAG-tagged M566T human TLK1 variant 5 in a retrovirus vector.

Depositor

Travis Stracker

Insert

TLK1 M566T (TLK1 Human)

Use

Tags

FLAG-STREP

Expression

Mammalian

Mutation

M566T

Promoter

CMV

Available since

July 24, 2024

Availability

Academic Institutions and Nonprofits only

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pUC18-LucFF

Plasmid

#220496

Purpose

To generate substrates to monitor Ku overloading and its impact on transcription at the DNA ends vicinity.

Depositor

Sébastien Britton

Article

Bossaert et al bioRxiv [Preprint]. 2024 Jan 4:2024.01.0

Insert

Firefly Luciferase

Use

Tags

Expression

Mammalian

Mutation

Promoter

SV40

Available since

July 3, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pLX-EF1a_NKX2-1_W208L

Plasmid

#221486

Purpose

NKX2-1 W208L DNA binding mutation lentiviral overexpression

Depositor

Matthew Meyerson

Article

Pulice et al bioRxiv [Preprint]. 2023 Oct 27:2023.10.

Insert

NKX2-1 (NKX2-1 Human)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Mutation

W208L

Promoter

EF1a

Available since

July 2, 2024

Availability

Academic Institutions and Nonprofits only

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pLX-UBC_NKX2-1

Plasmid

#221495

Purpose

NKX2-1 short isoform lentiviral overexpression using UBC promoter

Depositor

Matthew Meyerson

Article

Pulice et al bioRxiv [Preprint]. 2023 Oct 27:2023.10.

Insert

NKX2-1 (NKX2-1 Human)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Mutation

Promoter

UBC

Available since

July 2, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pDONR223_NKX2-1_Long

Plasmid

#221471

Purpose

Gateway cloning of NKX2-1 long isoform overexpression

Depositor

Matthew Meyerson

Article

Pulice et al bioRxiv [Preprint]. 2023 Oct 27:2023.10.

Insert

NKX2-1 (NKX2-1 Human)

Use

Gateway donor vector

Tags

Expression

Mutation

Promoter

Available since

July 2, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pDONR223_NKX2-1_W208L

Plasmid

#221474

Purpose

Gateway cloning of NKX2-1 W208L DNA binding mutation overexpression

Depositor

Matthew Meyerson

Article

Pulice et al bioRxiv [Preprint]. 2023 Oct 27:2023.10.

Insert

NKX2-1 (NKX2-1 Human)

Use

Gateway donor vector

Tags

Expression

Mutation

W208L

Promoter

Available since

July 2, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pDONR223_NKX2-1_V205F

Plasmid

#221473

Purpose

Gateway cloning of NKX2-1 V205F DNA binding mutation overexpression

Depositor

Matthew Meyerson

Article

Pulice et al bioRxiv [Preprint]. 2023 Oct 27:2023.10.

Insert

NKX2-1 (NKX2-1 Human)

Use

Gateway donor vector

Tags

Expression

Mutation

V205F

Promoter

Available since

July 2, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pLX-EF1a_NKX2-1_V205F

Plasmid

#221485

Purpose

NKX2-1 V205F DNA binding mutation lentiviral overexpression

Depositor

Matthew Meyerson

Article

Pulice et al bioRxiv [Preprint]. 2023 Oct 27:2023.10.

Insert

NKX2-1 (NKX2-1 Human)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Mutation

V205F

Promoter

EF1a

Available since

July 1, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pLX-hPGK_NKX2-1

Plasmid

#221494

Purpose

NKX2-1 short isoform lentiviral overexpression using hPGK promoter

Depositor

Matthew Meyerson

Article

Pulice et al bioRxiv [Preprint]. 2023 Oct 27:2023.10.

Insert

NKX2-1 (NKX2-1 Human)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Mutation

Promoter

hPGK

Available since

July 1, 2024

Availability

Academic Institutions and Nonprofits only

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pLX-SFFV_NKX2-1

Plasmid

#221496

Purpose

NKX2-1 short isoform lentiviral overexpression using SFFV promoter

Depositor

Matthew Meyerson

Article

Pulice et al bioRxiv [Preprint]. 2023 Oct 27:2023.10.

Insert

NKX2-1 (NKX2-1 Human)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Mutation

Promoter

SFFV

Available since

July 1, 2024

Availability

Academic Institutions and Nonprofits only

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pUN1309 - pL2_pNOS-RLUC-tNOS_tOCS-FLUC-pD4H

Plasmid

#203901

Purpose

Reporter plasmid for the D4H promoter from C. roseus with firefly luciferase readout and Renilla luciferase normalization in a plant binary vector for Agrobacterium-mediated transformation.

Depositor

Carolyn Lee-Parsons

Insert

pD4H-FLUC-tOCS; pNOS-RLUC-tNOS

Use

Luciferase

Tags

Expression

Plant

Mutation

Promoter

Available since

June 6, 2024

Availability

Academic Institutions and Nonprofits only

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dSp-Rta 125-175

Plasmid

#99668

Purpose

Expresses dSp Cas9 fused to truncated RTA activation domain

Depositor

George Church

Article

Vora et al bioRxiv 2018. 298620

Insert

dCas9

Use

Tags

truncated RTA activation domain (125-175)

Expression

Mammalian

Mutation

dead Cas9

Promoter

Available since

May 28, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
dSp-p65 (1-100)

Plasmid

#99660

Purpose

Expresses dSp Cas9 fused to truncated p65 activation domain

Depositor

George Church

Article

Vora et al bioRxiv 2018. 298620

Insert

dCas9

Use

Tags

truncated p65 activation domain (1-100)

Expression

Mammalian

Mutation

dead Cas9

Promoter

Available since

May 28, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
dSp-p65 (50-150)

Plasmid

#99661

Purpose

Expresses dSp Cas9 fused to truncated p65 activation domain

Depositor

George Church

Article

Vora et al bioRxiv 2018. 298620

Insert

dCas9

Use

Tags

truncated p65 activation domain (50-150)

Expression

Mammalian

Mutation

dead Cas9

Promoter

Available since

May 28, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
dSp-Rta Full (1-190)

Plasmid

#99662

Purpose

Expresses dSp Cas9 fused to truncated RTA activation domain

Depositor

George Church

Article

Vora et al bioRxiv 2018. 298620

Insert

dCas9

Use

Tags

truncated RTA activation domain (1-190)

Expression

Mammalian

Mutation

dead Cas9

Promoter

Available since

May 28, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
dSp-Rta 75-190

Plasmid

#99663

Purpose

Expresses dSp Cas9 fused to truncated RTA activation domain

Depositor

George Church

Article

Vora et al bioRxiv 2018. 298620

Insert

dCas9

Use

Tags

truncated RTA activation domain (75-190)

Expression

Mammalian

Mutation

dead Cas9

Promoter

Available since

May 28, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
dSp-Rta 75-175

Plasmid

#99666

Purpose

Expresses dSp Cas9 fused to truncated RTA activation domain

Depositor

George Church

Article

Vora et al bioRxiv 2018. 298620

Insert

dCas9

Use

Tags

truncated RTA activation domain (75-175)

Expression

Mammalian

Mutation

dead Cas9

Promoter

Available since

May 28, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
dSp-p65 (150-261)

Plasmid

#99655

Purpose

Expresses dSp Cas9 fused to truncated p65 activation domain

Depositor

George Church

Article

Vora et al bioRxiv 2018. 298620

Insert

dCas9

Use

Tags

truncated p65 activation domain (150-261)

Expression

Mammalian

Mutation

dead Cas9

Promoter

Available since

May 28, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
dSp-p65 (100-261)

Plasmid

#99656

Purpose

Expresses dSp Cas9 fused to truncated p65 activation domain

Depositor

George Church

Article

Vora et al bioRxiv 2018. 298620

Insert

dCas9

Use

Tags

truncated p65 activation domain (100-261)

Expression

Mammalian

Mutation

dead Cas9

Promoter

Available since

May 28, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
dSp-p65 (200-261)

Plasmid

#99657

Purpose

Expresses dSp Cas9 fused to truncated p65 activation domain

Depositor

George Church

Article

Vora et al bioRxiv 2018. 298620

Insert

dCas9

Use

Tags

truncated p65 activation domain (200-261)

Expression

Mammalian

Mutation

dead Cas9

Promoter

Available since

May 28, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
dSp-p65 (1-200)

Plasmid

#99658

Purpose

Expresses dSp Cas9 fused to truncated p65 activation domain

Depositor

George Church

Article

Vora et al bioRxiv 2018. 298620

Insert

dCas9

Use

Tags

truncated p65 activation domain (1-200)

Expression

Mammalian

Mutation

dead Cas9

Promoter

Available since

May 28, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
dSp-p65 (1-150)

Plasmid

#99659

Purpose

Expresses dSp Cas9 fused to truncated p65 activation domain

Depositor

George Church

Article

Vora et al bioRxiv 2018. 298620

Insert

dCas9

Use

Tags

truncated p65 activation domain (1-150)

Expression

Mammalian

Mutation

dead Cas9

Promoter

Available since

May 28, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
MBP-3C-hTspan12LEL(118-218)_pAcGP67a

Plasmid

#216383

Purpose

Baculovirus transfer vector to secrete human Tspan12 LEL (residues 118-218) fused to MBP

Depositor

William Weis

Article

Bruguera et al bioRxiv 2024.02.03.578714

Insert

Tetraspanin-12 (TSPAN12 Human)

Use

Baculovirus

Tags

GP64 signal sequence-MBP-3C

Expression

Insect

Mutation

Promoter

Polyhedrin

Available since

May 13, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
RNF169 C-terminal sgRNA

Plasmid

#207099

Purpose

pX330 based plasmid for expression of Cas9 and the ACACTTCATTAGGTGCTACT sgRNA to target the RNF169 locus.

Depositor

Jens Schmidt

Article

Heyza et al Elife. 2023 Jun 21;12:e87086. doi: 10.75

Insert

ACACTTCATTAGGTGCTACT

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV and U6

Available since

May 6, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
SHLD2 N-terminal sgRNA

Plasmid

#207098

Purpose

pX330 based plasmid for expression of Cas9 and the TTTTTATCAGAAATCATGAG sgRNA to target the SHLD2 locus.

Depositor

Jens Schmidt

Article

Heyza et al Elife. 2023 Jun 21;12:e87086. doi: 10.75

Insert

TTTTTATCAGAAATCATGAG

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV and U6

Available since

April 10, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pCfB12720

Plasmid

#212704

Purpose

gRNA targeting to the gene 4HPPD locus

Depositor

Irina Borodina

Article

Jiang et al Metab Eng. 2024 Sep 9:S1096-7176(24)0011

Insert

gRNA targeting to the gene 4HPPD locus

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

April 3, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
Fg vector

Plasmid

#213464

Purpose

Destination vector for cloning and transformation into TSI locus 1.

Depositor

Kim Hammond-Kosack

Article

Darino et al Fungal Biol Biotechnol. 2024 Feb 26;11(1

Type

Empty backbone

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 29, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
MDC1 N-terminal sgRNA

Plasmid

#207090

Purpose

pX330 based plasmid for expression of Cas9 and the GTATCCTTCCCAGATCATGG sgRNA to target the MDC1 locus.

Depositor

Jens Schmidt

Article

Heyza et al Elife. 2023 Jun 21;12:e87086. doi: 10.75

Insert

GTATCCTTCCCAGATCATGG

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV and U6

Available since

March 28, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge

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