We narrowed to 5,415 results for: PEP;

1. pcDNA3.1(+)-OTI-TCRB_p2_TCRA

   Plasmid

   #131034

   Purpose

   Expresses OT-I TCRB linked to TCRA with the P2A self-cleaving peptide

   Depositor

   Jeff Hammerbacher

   Article

   Aksoy et al bioRxiv

   Inserts

   TCRB (Tcrb Mouse)
   TCRA (Tcra Mouse)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV/T7

   Available since

   Oct. 8, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pMT-Bip-GFP:V5:KDEL

   Plasmid

   #69917

   Purpose

   An endoplasmic reticulum (ER) marker used to visulaize the ER structure in Drosophilia S2 cells.

   Depositor

   Kenneth Irvine

   Article

   Okajima et al Science. 2005 Mar 11;307(5715):1599-603.

   Insert

   GFP
   

   Use

   Tags

   Drosophila BiP signal sequence, KDEL ER targeting…

   Expression

   Insect

   Mutation

   Promoter

   pMT

   Available since

   Nov. 3, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLVX-EF1a-PTS1-IRES-Puromycin

   Plasmid

   #134665

   Purpose

   Expresses EGFP-tagged peroxisome marker

   Depositor

   David Andrews

   Article

   Schormann et al J Cell Biol. 2020 Mar 2;219(3). pii: 133

   Insert

   tripeptide SKL (Peroxisome targeting signal 1)
   

   Use

   Lentiviral

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   March 2, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pYHY3

   Plasmid

   #216109

   Purpose

   aTC-inducible expression of secretory BT3630 SP-NanoLuc via P1TDP-A21 promoter-RBS pair in Bacteroides species.

   Depositor

   Shannon Sirk

   Article

   Yeh et al Nat Commun. 2024 Nov 11;15(1):9741. doi:

   Insert

   NanoLuc
   

   Use

   Synthetic Biology; E. coli-bacteroides shuttle ve…

   Tags

   3xFLAG tag and BT3630 signal peptide

   Expression

   Bacterial

   Mutation

   Promoter

   P1TDP-A21

   Available since

   May 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCIS.MTRIA-NPFF

   Plasmid

   #184612

   Purpose

   For expression of a fluorescent sensor for neuropeptide FF in mammalian cells

   Depositor

   Daisuke Ino, Hiroshi Hibino

   Article

   Ino et al Nat Methods. 2022 Sep 22. pii: 10.1038/s

   Insert

   MTRIA-NPFF
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG promoter

   Available since

   June 15, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. hPDI1_18-508_WT_pFLAG-CMV1

   Plasmid

   #31382

   Depositor

   David Ron

   Article

   Zito et al Mol Cell. 2010 Dec 10. 40(5):787-97.

   Insert

   hPDI1 (P4HB Human)
   

   Use

   Tags

   FLAG M2

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 23, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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7. FLAG-3C-sGFP11_pcDNA3.1(-)

   Plasmid

   #182932

   Purpose

   For expression of membrane protein with N-terminal FLAG tag and C-terminal split GFP11 fragment

   Depositor

   William Weis

   Article

   Bruguera et al J Biol Chem. 2022 Jan 21:101628. doi: 10

   Type

   Empty backbone

   Use

   Tags

   3C-cleavable split GFP 11, FLAG tag, and HA signa…

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   April 15, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. FLAG-3C-sGFP1-10_pcDNA3.1(-)

   Plasmid

   #182931

   Purpose

   For expression of membrane protein with N-terminal FLAG tag and C-terminal split GFP1-10 fragment

   Depositor

   William Weis

   Article

   Bruguera et al J Biol Chem. 2022 Jan 21:101628. doi: 10

   Type

   Empty backbone

   Use

   Tags

   3C-cleavable split GFP 1-10, FLAG tag, and HA sig…

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   April 15, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pcDNA5-AP-Ub-dGG

   Plasmid

   #232581

   Purpose

   Express Acceptor Peptide (AP) tagged Ubiquitin lacking the two terminal glycines

   Depositor

   Sagar Bhogaraju

   Article

   Mukhopadhyay et al Sci Adv. 2024 Aug 9;10(32):eadp3000. doi

   Insert

   Ubiquitin
   

   Use

   Tags

   AviTag

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 11, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCI-neo Flag HAUSP

    Plasmid

    #16655

    Depositor

    Bert Vogelstein

    Article

    Cummins et al Cell Cycle. 2004 Jun . 3(6):689-92.

    Insert

    HAUSP (USP7 Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 7, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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11. pQE80L SpyTag-ELP-SpyTag

    Plasmid

    #112634

    Purpose

    Two SpyTags linked by an elastin-like polypeptide for hydrogel formation

    Depositor

    Mark Howarth

    Article

    Wieduwild et al Biomaterials. 2018 Oct;180:253-264. doi:

    Insert

    SpyTag-ELP-SpyTag
    

    Use

    Tags

    His6

    Expression

    Bacterial

    Mutation

    central cysteine

    Promoter

    T5

    Available since

    Sept. 7, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pEFIRES-P-mTagBFP-KDEL

    Plasmid

    #87163

    Purpose

    Fluorescent marker of endoplasmic reticulum (ER)

    Depositor

    Elina Ikonen

    Article

    Salo et al EMBO J. 2016 Dec 15;35(24):2699-2716. Ep

    Insert

    mTagBFP-KDEL
    

    Use

    Tags

    Bip signal peptide (Homo sapiens) and mTagBFP-KDE…

    Expression

    Mammalian

    Mutation

    Promoter

    EF-1 alpha

    Available since

    May 30, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCRISPR-S12

    Plasmid

    #84031

    Purpose

    To episomally express codon optimized Cas9 and chimeric guide RNA

    Depositor

    Sen Wu

    Article

    Li et al Protein Cell. 2016 Sep;7(9):689-91. doi:

    Inserts

    hSpCas9
    eGFP
    

    Use

    Tags

    3x FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV and CMV (downstream of F2A self-cleaving pept…

    Available since

    Aug. 28, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. pNM262

    Plasmid

    #128412

    Purpose

    Vanillic acid inducible production of lysostaphin. LsyT with the amyE signaling peptide for secretion.

    Depositor

    Christopher Voigt

    Article

    Gonzalez et al Nat Chem Biol. 2019 Dec 2. pii: 10.1038/

    Insert

    Lysostaphin (epr Staphylococcus simulans)
    

    Use

    Synthetic Biology

    Tags

    amyE SP

    Expression

    Bacterial

    Mutation

    Promoter

    Pspank(V)

    Available since

    Sept. 2, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pME-GFP-P2A

    Plasmid

    #80807

    Purpose

    GFP without stop codon followed by porcine teschovirus-1 2A (P2A) "self-cleaving" peptide

    Depositor

    Philip Washbourne

    Article

    Fowler et al PLoS One. 2016 Aug 8;11(8):e0159277. doi

    Insert

    GFP-P2A
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 1, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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16. huntingtin_FL_NbioCflag

    Plasmid

    #194767

    Purpose

    Baculovirus transfer vector for expression of huntingtin protein in insect and mammalian cells

    Depositor

    Cheryl Arrowsmith

    Insert

    HTT (HTT Human)
    

    Use

    Baculovirus expression

    Tags

    Avi-tag (biotin acceptor peptide) and Flag-tag

    Expression

    Mammalian

    Mutation

    Q23 (polyQ repeat)

    Promoter

    CMV and P10

    Available since

    July 27, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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17. pIBA2-Int-SpyTag

    Plasmid

    #198038

    Purpose

    Expression plasmid for producing SpyTagged stalled secretion variant of intimin

    Depositor

    Jack Leo

    Article

    Duodu et al Methods Mol Biol. 2024;2778:53-63. doi:

    Insert

    eaeA
    

    Use

    Tags

    SpyTag

    Expression

    Bacterial

    Mutation

    lacks native signal peptide (amino acids 1-39)

    Promoter

    tet

    Available since

    March 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pSkipFlox

    Plasmid

    #85797

    Purpose

    P. falciparum plasmid for expression of both diCre fragments from single casette using skip peptide

    Depositor

    Tobias Spielmann

    Article

    Birnbaum et al Nat Methods. 2017 Mar 13. doi: 10.1038/n

    Insert

    split Cre recombinase
    

    Use

    Cre/Lox; P. falciparum expression

    Tags

    FKBP, FRB*

    Expression

    Mutation

    Promoter

    Pfcrt

    Available since

    March 15, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. CMV-2mCh-PS

    Plasmid

    #112268

    Purpose

    IDOCKS Acceptor Construct

    Depositor

    Ryohei Yasuda

    Article

    Colgan et al Nat Neurosci. 2018 Aug;21(8):1027-1037.

    Insert

    two copies of mCherry followed by a pseudosubstrate peptide for PKC
    

    Use

    Tags

    mCh

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. pET28a-BCA-Q11

    Plasmid

    #185781

    Purpose

    Encodes enzyme bovine carbonic anhydrase linked to Q11 self-assembling peptide via a GS-linker sequence

    Depositor

    Lizhong He

    Article

    Shanbhag et al Adv. Funct. Mater. 2022, 32, 2108039

    Insert

    BCA-Q11
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    July 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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