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Showing: 3661 - 3680 of 64328 results

  2. pMD19T-psba1-Ppsba2-dCas9-SpR

    Plasmid
    #73220
    Purpose
    Contains dCas9 from S. pyogenes under constitutive promoter. Suicide vector inserts into psba1 site of Synechocystis. Carries spectinomycin resist. Recommend E. coli Copy cutter for propogation.
    Depositor
    Paul Hudson
    Article
    Yao et al ACS Synth Biol. 2015 Dec 28.
    Insert
    dCas9 from S. pyogenes
    Tags
    c-myc
    Expression
    Bacterial
    Mutation
    Silent mutation at bp 1341 A->C to remove an E…
    Promoter
    PpsbA2
    Available Since
    March 15, 2016
    Availability
    Academic Institutions and Nonprofits only
    73220-plasmid-map-sequence-id-134711

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  4. pCJ136

    Plasmid
    #162665
    Purpose
    pET-21b(+) based plasmid for expression of MHETase from Ideonella sakaiensis 201-F6 (Genbank GAP38911.1), codon optimized for expression in E. coli K12, with C-terminal His tag.
    Depositor
    Gregg Beckham
    Article
    Knott et al Proc Natl Acad Sci U S A. 2020 Oct 13;11
    Insert
    MHETase from Ideonella sakaiensis 201-F6 (Genbank GAP38911.1)
    Tags
    His
    Expression
    Bacterial
    Mutation
    codon optimized for expression in E. coli K12
    Promoter
    T7/lac
    Available Since
    Jan. 7, 2021
    Availability
    Academic Institutions and Nonprofits only
    162665-plasmid-map-sequence-id-321451

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  7. Spike Display_B.1.1.7

    Plasmid
    #172735
    Purpose
    Expression vector for SARS-CoV-2 (B.1.1.7) spike used in the Spike Display system
    Depositor
    IIya Finkelstein
    Article
    Javanmardi et al Mol Cell. 2021 Dec 16;81(24):5099-5111.e
    Insert
    SARS-CoV-2 S B.1.1.7 (S Severe acute respiratory syndrome coronavirus 2)
    Tags
    3X-FLAG; Strep-Tag II; HRV 3C cut site; PDGFR-B T…
    Expression
    Mammalian
    Mutation
    Ectodomain only (AAs 1-1208); 682-685 (furin site…
    Promoter
    CMV
    Available Since
    Aug. 17, 2021
    Availability
    Industry, Academic Institutions, and Nonprofits
    172735-plasmid-map-sequence-id-340032

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  9. pSW031

    Plasmid
    #231144
    Purpose
    Used to express cyclic sortase cW11 (recognizes APXTG motif) from E. coli.
    Depositor
    Philip Cole
    Article
    Whedon et al J Am Chem Soc. 2024 Nov 25. doi: 10.1021
    Insert
    S. aureus Sortase A mutant
    Tags
    Nostoc punctiform split intein C-terminal fragmen…
    Expression
    Bacterial
    Mutation
    P94S, E108Q, S116V, D160N, T164Q, V168M, L169H, D…
    Promoter
    T7
    Available Since
    Feb. 3, 2025
    Availability
    Academic Institutions and Nonprofits only
    231144-plasmid-map-sequence-id-460063

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Showing: 3661 - 3680 of 64328 results