We narrowed to 88,648 results for: Mal;

1. pU6-mir451 EF1Alpha-puro-T2A-BFP

   Plasmid

   #164792

   Purpose

   Express miR-451 under the U6 promoter in mammalian cells

   Depositor

   David Bartel

   Article

   Fang et al Mol Cell. 2020 Apr 16;78(2):289-302.e6.

   Inserts

   microRNA 451a (MIR451A Human)
   puro-T2A-BFP
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   EF1Alpha and U6

   Available Since

   March 29, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. peGFP C3-SPG20-PAAA

   Plasmid

   #218581

   Purpose

   Expresses GFP-tagged PPAY motif mutant (PAAA) of human SPG20 in mammalian cells; this mutant does not interact with ITCH

   Depositor

   Hemmo Meyer

   Article

   Gahlot et al Mol Cell. 2024 Mar 14:S1097-2765(24)0017

   Insert

   SPG20 (SPART Human)
   

   Tags

   eGFP

   Expression

   Mammalian

   Mutation

   changed Proline 172 to Alanine and Tyrosine 174 t…

   Promoter

   CMV

   Available Since

   May 6, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCAG-SpCas9-GFP-U6-gRNA

   Plasmid

   #79144

   Purpose

   All-in-one CRISPR/Cas9 vector with CAG promoter for expression in human ESC/iPSC

   Depositor

   Jizhong Zou

   Article

   Stoehr et al JCI Insight. 2019 May 21;5. pii: 128496.

   Insert

   SpCas9
   

   Use

   CRISPR

   Tags

   2A-GFP

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   June 29, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. PDG458 V3

   Plasmid

   #226959

   Purpose

   CBh-SpCas9-2A-GFP, and 2X hU6-sgRNA (Sp) with BbsI golden gate cloning backbone dual gRNAs. sgRNA uses 3TC scaffold for increased sgRNA expression.

   Depositor

   Paul Thomas

   Article

   Chey et al BMC Genomics. 2025 Feb 12;26(1):138. doi

   Type

   Empty backbone

   Use

   CRISPR

   Expression

   Mammalian

   Available Since

   Oct. 30, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. GFP-Rab27B

   Plasmid

   #89447

   Purpose

   Expression of GFP-tagged human Rab27B in mammalian cells

   Depositor

   Wendy Westbroek

   Article

   Hendrix et al J Natl Cancer Inst. 2010 Jun 16;102(12):

   Insert

   human Rab27B (RAB27B Human)
   

   Tags

   eGFP

   Expression

   Mammalian

   Mutation

   wild type

   Promoter

   CMV

   Available Since

   April 24, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. NKP38 GFP (1009)

   Plasmid

   #62021

   Purpose

   mammalian expression of nuclear envelope transmembrane protein

   Depositor

   Eric Schirmer

   Article

   Korfali et al Nucleus. 2012 Nov-Dec;3(6):552-64. doi:

   Insert

   AYTL1 (LPCAT2 Human)
   

   Tags

   EGFP

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Jan. 28, 2015

   Availability

   Academic Institutions and Nonprofits only

7. PX458 V3

   Plasmid

   #226957

   Purpose

   CBh-SpCas9-2A-GFP, and hU6-sgRNA (Sp) with a BbsI golden gate cloning backbone for sgRNA. sgRNA uses 3TC scaffold for increased sgRNA expression.

   Depositor

   Paul Thomas

   Article

   Chey et al BMC Genomics. 2025 Feb 12;26(1):138. doi

   Type

   Empty backbone

   Use

   CRISPR

   Expression

   Mammalian

   Available Since

   Oct. 30, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCherryGFP-CoV2-Inframe-stop

   Plasmid

   #177620

   Purpose

   Dual fluorescent protein reporter for SARS-CoV-2 programmed -1 ribosomal frameshifting. GFP is in-frame with mCherry. Thus GFP translation is prevented by stop codon within FSE (negative control).

   Depositor

   Junjie Guo

   Article

   Sun et al Proc Natl Acad Sci U S A. 2021 Jun 29;11

   Insert

   SARS-CoV-2 FSE
   

   Use

   Lentiviral

   Expression

   Mammalian

   Available Since

   Dec. 10, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pTBK1

   Plasmid

   #87443

   Purpose

   express TBK1 in mammalian cells

   Depositor

   Georgios Stathopoulos

   Article

   Marazioti et al Nat Commun. 2018 Feb 14;9(1):672. doi: 1

   Insert

   TBK1 (Tbk1 Mouse)
   

   Expression

   Mammalian

   Available Since

   May 31, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAAV-CKIIa-stGtACR2-FusionRed

    Plasmid

    #105669

    Purpose

    Expresses a soma-targeted GtACR2 in mammalian neurons under the CaMKIIa promoter

    Depositor

    Ofer Yizhar

    Article

    Mahn et al Nat Commun. 2018 Oct 8;9(1):4125. doi: 1

    Has Service

    AAV Retrograde and AAV1

    Insert

    stGtACR2
    

    Use

    AAV

    Tags

    FusionRed and Kv2.1

    Expression

    Mammalian

    Promoter

    CaMKIIa

    Available Since

    Feb. 23, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pLEX-Frb:Akt2-IRES-Hygromycin

    Plasmid

    #120713

    Purpose

    Expresses Frb:Akt2 fusion protein in mammalian cells & for virus production

    Depositor

    Paul Khavari

    Article

    Kovalski et al Mol Cell. 2019 Jan 4. pii: S1097-2765(18

    Insert

    AKT2 (AKT2 Human)
    

    Use

    Lentiviral

    Tags

    FRB(T2098L)

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 6, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. TPC2-D276K-G-GECO1.2

    Plasmid

    #207142

    Purpose

    Fusion of Ca2+ reporter and pore-dead lysosomal Ca2+-permeable channel

    Depositor

    Antony Galione

    Article

    Davis et al EMBO J. 2020 Jul 15;39(14):e104058. doi:

    Insert

    TPC2N2 (TPCN2 Human)
    

    Tags

    G-GECO1.2

    Expression

    Mammalian

    Mutation

    Changed Aspartate 276 to Lysine

    Promoter

    CMV

    Available Since

    Nov. 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pLenti_Ef1a:PE2-P2A-GFP

    Plasmid

    #184445

    Purpose

    Single EF1a-driven PE2-P2A-GFP (Addgene #132776) and PaqCI restriction sites for pegRNA cloning. Lentiviral backbone and AmpR.

    Depositor

    Bobby Koeleman

    Article

    Dirkx et al BMC Biol. 2023 Jul 13;21(1):156. doi: 10

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    U6 and Ef1A

    Available Since

    Oct. 18, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. WN10150

    Plasmid

    #80443

    Purpose

    Expresses dead (908A) AsCpf1

    Depositor

    Ervin Welker

    Article

    Toth et al Nucleic Acids Res. 2018 Sep 20. pii: 510

    Insert

    nuclease dead (908A) AsCpf1
    

    Tags

    NLS-3xHA

    Expression

    Mammalian

    Mutation

    nuclease dead (D908A)

    Available Since

    Aug. 29, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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15. Il12b 400bp pro-Luc

    Plasmid

    #20020

    Depositor

    Stephen Smale

    Article

    Plevy et al Mol Cell Biol. 1997 Aug . 17(8):4572-88.

    Insert

    Il12b promoter (Il12b Mouse)
    

    Use

    Luciferase

    Expression

    Mammalian

    Available Since

    Jan. 28, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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16. PD-1-miSFIT-4x

    Plasmid

    #124678

    Purpose

    Tuning expression of PD-1

    Depositor

    Tudor Fulga

    Article

    Michaels et al Nat Commun. 2019 Feb 18;10(1):818. doi:

    Insert

    PD-1 (PDCD1 Human)
    

    Expression

    Mammalian

    Promoter

    minimal CMV

    Available Since

    May 7, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. pLOVE-Nmyc

    Plasmid

    #15951

    Depositor

    Miguel Ramalho-Santos

    Article

    Blelloch et al Cell Stem Cell. 2007 Sep 13;1(3):245-7.

    Insert

    v-myc myelocytomatosis viral related oncogene, neuroblastoma derived (avian) (Mycn Mouse)
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mammalian

    Mutation

    The recombination reaction required to clone &quo…

    Available Since

    Nov. 7, 2007

    Availability

    Academic Institutions and Nonprofits only

18. pINDUCER-21-SPI1

    Plasmid

    #97039

    Purpose

    Expresses SPI1 in mammalian cells

    Depositor

    George Daley

    Article

    Sugimura et al Nature. 2017 May 17. doi: 10.1038/nature

    Insert

    Spi-1 Proto-Oncogene (SPI1 Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    Tet on

    Available Since

    Sept. 5, 2017

    Availability

    Academic Institutions and Nonprofits only

19. pCAGGS-PIF3-mEGFP

    Plasmid

    #100283

    Purpose

    Expresses PIF3 (1-100 aa) fused with mEGFP in mammalian cells

    Depositor

    Kazuhiro Aoki

    Article

    Uda et al Proc Natl Acad Sci U S A. 2017 Oct 24. p

    Insert

    Phytochrome interacting factor 3 (PIF3 Mustard Weed)
    

    Tags

    mEGFP

    Expression

    Mammalian

    Mutation

    N-terminus (1-100 aa) of PIF3 is used

    Promoter

    CAG promoter

    Available Since

    Sept. 20, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. FUGW-RUSH-LAMP2A-mNeonGreen

    Plasmid

    #228525

    Purpose

    Lentiviral vector, expresses Strep-KDEL-IRES-SBP-(Rat)LAMP2A-mNeonGreen in mammalian cells.

    Depositor

    Ginny Farias

    Article

    Li et al Nat Commun. 2024 Dec;15: 10829. doi: 10.

    Insert

    LAMP2 (Lamp2 Rat)
    

    Use

    Lentiviral

    Tags

    Streptavidin Binding Protein (SBP), Streptavidin-…

    Expression

    Mammalian

    Promoter

    Ubc

    Available Since

    Feb. 27, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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