4,179 results

1. pcDNA 3.1(-) mouse C/EBP beta (LAP)

   Plasmid

   #12557

   Depositor

   Peter Johnson

   Insert

   CCAAT/Enhancer-binding Protein beta (Cebpb Mouse)
   

   Expression

   Mammalian

   Available Since

   July 18, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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2. pSico PGK puro

   Plasmid

   #11586

   Purpose

   Cre-regulated lentiviral shRNA vector. Cre addition causes the puromycin gene to be recombined out of the construct, activating shRNA expression.

   Depositor

   Tyler Jacks

   Article

   Ventura et al Proc Natl Acad Sci U S A. 2004 Jul 13. 1

   Type

   Empty backbone

   Use

   Cre/Lox, Lentiviral, and RNAi

   Expression

   Mammalian

   Available Since

   July 19, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCMVTAG-NEMO

   Plasmid

   #11970

   Depositor

   Jon Ashwell

   Article

   Wu et al Nat Cell Biol. 2006 Apr . 8(4):398-406.

   Insert

   NEMO (IKBKG Human)
   

   Tags

   Flag

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Sept. 21, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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4. FUmGW

   Plasmid

   #22479

   Depositor

   Connie Cepko

   Article

   Rompani et al Proc Natl Acad Sci U S A. 2008 Jan 8. 10

   Insert

   membrane-bound green fluorescent protein
   

   Use

   Lentiviral

   Tags

   palmitoylation

   Expression

   Mammalian

   Available Since

   Nov. 5, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLVX-EF1alpha-SARS-CoV-2-orf6-2xStrep-IRES-Puro

   Plasmid

   #141387

   Purpose

   Lentiviral expression of SARS-CoV-2 protein; transient expression and generate lentivirus

   Depositor

   Nevan Krogan

   Article

   Gordon et al Nature. 2020 Jul;583(7816):459-468. doi:

   Insert

   SARS-CoV-2-orf6 (ORF6 SARS-CoV-2)
   

   Use

   Lentiviral

   Tags

   2xStrep

   Expression

   Mammalian

   Mutation

   human codon optimized

   Promoter

   EF1alpha

   Available Since

   April 9, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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6. pDONR223 SARS-CoV-2 S

   Plasmid

   #149329

   Purpose

   Gateway-compatible Entry vector, with insert of the spike (S) gene's CDS from SARS-CoV-2 isolate Wuhan-Hu-1

   Depositor

   Fritz Roth

   Article

   Kim et al G3 (Bethesda). 2020 Aug 6. pii: g3.120.4

   Insert

   S (S SARS-CoV-2 isolate Wuhan-Hu-1)
   

   Use

   Gateway-compatible entry vector

   Mutation

   Many synonymous changes due to codon optimization

   Available Since

   May 6, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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7. phOCT4-Luc

   Plasmid

   #17221

   Depositor

   Shinya Yamanaka

   Article

   Takahashi et al Cell. 2007 Nov 30. 131(5):861-72.

   Insert

   Upstream region of human OCT4 (POU5F1 Human)
   

   Use

   Luciferase

   Expression

   Mammalian

   Available Since

   Feb. 8, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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8. pcDNA3.1-CibN-dCas9-CibN

   Plasmid

   #60553

   Purpose

   Expresses CibN-dCas9-CibN in mammalian cells

   Depositor

   Charles Gersbach

   Article

   Polstein et al Nat Chem Biol. 2015 Feb 9. doi: 10.1038/

   Insert

   dCas9-CibN
   

   Use

   CRISPR

   Tags

   FLAG and SV40 NLS

   Expression

   Mammalian

   Mutation

   inactivating Cas9 mutations D10A and H840A

   Promoter

   CMV

   Available Since

   Feb. 2, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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9. pcDNA3-myc3-Cul7

   Plasmid

   #20695

   Depositor

   Yue Xiong

   Article

   Andrews et al Oncogene. 2006 Aug 3. 25(33):4534-48.

   Insert

   cullin 7 (CUL7 Human)
   

   Tags

   Myc3

   Expression

   Mammalian

   Available Since

   April 14, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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10. pLVX-EF1alpha-SARS-CoV-2-nsp4-2xStrep-IRES-Puro

    Plasmid

    #141369

    Purpose

    Lentiviral expression of SARS-CoV-2 protein; transient expression and generate lentivirus

    Depositor

    Nevan Krogan

    Article

    Gordon et al Nature. 2020 Jul;583(7816):459-468. doi:

    Insert

    SARS-CoV-2-nsp4 (ORF1ab SARS-CoV-2)
    

    Use

    Lentiviral

    Tags

    2xStrep

    Expression

    Mammalian

    Mutation

    human codon optimized

    Promoter

    EF1alpha

    Available Since

    April 8, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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11. pSK+KanaRpsL

    Plasmid

    #20871

    Depositor

    Jiyue Zhu

    Article

    Wang et al Mol Biotechnol. 2009 May;42(1):110-6. do

    Insert

    KanamycinResistance-RpsL
    

    Expression

    Bacterial

    Mutation

    none

    Available Since

    May 5, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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12. pLVX-EF1alpha-SARS-CoV-2-orf9b-2xStrep-IRES-Puro

    Plasmid

    #141392

    Purpose

    Lentiviral expression of SARS-CoV-2 protein; transient expression and generate lentivirus

    Depositor

    Nevan Krogan

    Article

    Gordon et al Nature. 2020 Jul;583(7816):459-468. doi:

    Insert

    SARS-CoV-2-orf9b
    

    Use

    Lentiviral

    Tags

    2xStrep

    Expression

    Mammalian

    Mutation

    human codon optimized

    Promoter

    EF1alpha

    Available Since

    April 8, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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13. GFP-BLM

    Plasmid

    #80070

    Purpose

    Expresses GFP-tagged BLM helicase in mammalian cells (EGFP-C1 backbone)

    Depositor

    Nathan Ellis

    Article

    Hu et al Hum Mol Genet. 2001 Jun 1;10(12):1287-98

    Insert

    BLM (BLM Human)
    

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    If correct ID, the cDNA is from HeLa and has char…

    Available Since

    July 12, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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14. HA PKB T308D S473D pcDNA3

    Plasmid

    #14751

    Depositor

    Jim Woodgett

    Article

    Scheid et al Mol Cell Biol. 2002 Sep . 22(17):6247-60

    Insert

    PKB DD (AKT1 Bovine)
    

    Tags

    HA

    Expression

    Mammalian

    Mutation

    T308D S473D. Constitutively active mutant.

    Available Since

    April 12, 2007

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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15. pYPet-His

    Plasmid

    #14031

    Depositor

    Patrick Daugherty

    Article

    Nguyen et al Nat Biotechnol. 2005 Mar;23(3):355-60. d

    Insert

    YPet
    

    Tags

    6xHis

    Expression

    Bacterial

    Available Since

    Feb. 14, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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16. pLNCX chick src Y527F

    Plasmid

    #13660

    Depositor

    Joan Brugge

    Insert

    src Y527F (SRC Chicken)
    

    Use

    Retroviral

    Expression

    Mammalian

    Mutation

    Y527F. Constitutive active mutant.

    Available Since

    Jan. 4, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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17. pAV5S-F30-2xdBroccoli

    Plasmid

    #66845

    Purpose

    Expresses 5S rRNA fused to two dimeric Broccoli aptamers in the F30 scaffold in mammalian cells

    Depositor

    Samie Jaffrey

    Article

    Filonov et al Curr Protoc Chem Biol. 2016 Mar 16;8(1):

    Insert

    Two dimeric Broccoli aptamers in the F30 scaffold
    

    Tags

    5S rRNA

    Expression

    Mammalian

    Promoter

    5S

    Available Since

    June 25, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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18. TetO-FUW-oct4

    Plasmid

    #20323

    Depositor

    Rudolf Jaenisch

    Article

    Brambrink et al Cell Stem Cell. 2008 Feb 7. 2(2):151-9.

    Insert

    oct4 (Pou5f1 Mouse)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    March 13, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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19. pK-myc-Par3b

    Plasmid

    #19388

    Depositor

    Ian Macara

    Article

    Joberty et al Nat Cell Biol 2000 Aug;2(8):531-9.

    Insert

    Partitioning-defective protein 3b (PARD3 Human)
    

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    Full length, isoform b.

    Available Since

    March 31, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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20. pMXs-puro GFP-DFCP1

    Plasmid

    #38269

    Depositor

    Noboru Mizushima

    Article

    Itakura et al Autophagy. 2010 Aug;6(6):764-76.

    Insert

    Zinc finger FYVE domain-containing protein 1 (Zfyve1 Mouse)
    

    Use

    Retroviral

    Tags

    EGFP

    Expression

    Mammalian

    Available Since

    Aug. 16, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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