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pX330-Cetn1/1

Plasmid

#50718

Purpose

pX330 containing sgRNA against mouse Cent1. Positive control for DSB mediated EGFP reconstitution.

Depositor

Masahito Ikawa

Article

Mashiko et al Sci Rep. 2013 Nov 27;3:3355. doi: 10.103

Inserts

Cetn1 sgRNA1
humanized S. pyogenes Cas9

Use

CRISPR

Tags

3xFLAG

Expression

Mammalian

Mutation

Promoter

CBh and hU6

Available since

Feb. 7, 2014

Availability

Academic Institutions and Nonprofits only

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pcDNA3-mNeptune2-N

Plasmid

#41645

Purpose

Expresses far-red FP mNeptune2 fused to Dronpa145N. Dronpa145N oligomerizes under violet light (390-400nm) and dissociates under cyan light (490-500nm)

Depositor

Michael Lin

Article

Zhou et al Science. 2012 Nov 9;338(6108):810-4. doi

Insert

mNeptune2-Dronpa145N

Use

Tags

Expression

Mammalian

Mutation

K145N on Dronpa (allows control of photochromic F…

Promoter

CMV

Available since

Dec. 21, 2012

Availability

Academic Institutions and Nonprofits only

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809 pcDNA3 GFP PTEN

Plasmid

#10759

Depositor

William Sellers

Article

Vazquez et al J Biol Chem. 2001 Dec 28. 276(52):48627-

Insert

PTEN (PTEN Human)

Use

Tags

GFP

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 13, 2006

Availability

Academic Institutions and Nonprofits only

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pCS2-MT GLI2 FL

Plasmid

#17648

Depositor

Erich Roessler

Article

Roessler et al Hum Mol Genet. 2005 Aug 1. 14(15):2181-8

Insert

GLI2 (GLI2 Human)

Use

Tags

6x Myc

Expression

Mammalian

Mutation

Promoter

CMV IE94

Available since

March 27, 2008

Availability

Academic Institutions and Nonprofits only

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B23(NPM)

Plasmid

#34553

Depositor

Mary Dasso

Article

Yun et al J Cell Biol. 2008 Nov 17;183(4):589-95.

Insert

B23/nucleophosmin (NPM1 Human)

Use

Tags

DsRed

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Dec. 14, 2011

Availability

Academic Institutions and Nonprofits only

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pGEX-4T1 SHP2 WT

Plasmid

#8322

Depositor

Ben Neel

Article

O'Reilly et al Mol Cell Biol 2000 Jan;20(1):299-311.

Insert

SHP2 (PTPN11 Human)

Use

Tags

GST

Expression

Bacterial

Mutation

Promoter

Available since

March 14, 2006

Availability

Academic Institutions and Nonprofits only

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pmStrawberry-Atg4BC74A

Plasmid

#21076

Depositor

Tamotsu Yoshimori

Article

Fujita et al Mol Biol Cell. 2008 Nov . 19(11):4651-9.

Insert

Atg4B(C74A) (Atg4b Mouse)

Use

Tags

mStrawberry

Expression

Mammalian

Mutation

220-222 TGC(Cys) is changed to GCA (Ala)

Promoter

Available since

June 8, 2009

Availability

Academic Institutions and Nonprofits only

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pcDNA3-ICUE3

Plasmid

#61622

Purpose

FRET-based biosensor for monitoring cAMP

Depositor

Jin Zhang

Article

DiPilato et al Mol Biosyst. 2009 Aug;5(8):832-7. doi: 1

Insert

ICUE3 (RAPGEF3 Human)

Use

Tags

ECFP and cpVenus[L194]

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 13, 2015

Availability

Academic Institutions and Nonprofits only

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Orai1 CFP

Plasmid

#19757

Depositor

Anjana Rao

Article

Prakriya et al Nature. 2006 Sep 14. 443(7108):230-3.

Insert

Orai1 (ORAI1 Human)

Use

Tags

CFP

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 12, 2008

Availability

Academic Institutions and Nonprofits only

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pCMV-P35

Plasmid

#1347

Depositor

Li-Huei Tsai

Insert

P35 (CDK5R1 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 18, 2006

Availability

Academic Institutions and Nonprofits only

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Flag-hPIASy

Plasmid

#15208

Depositor

Ke Shuai

Article

Liu et al Proc Natl Acad Sci U S A. 2001 Mar 13. 9

Insert

PIASy (PIAS4 Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 8, 2008

Availability

Academic Institutions and Nonprofits only

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pMIG-Aalpha WT

Plasmid

#10884

Depositor

William Hahn

Article

Chen et al Cancer Res. 2005 Sep 15. 65(18):8183-92.

Insert

PP2A Aalpha (PPP2R1A Human)

Use

Retroviral

Tags

Flag

Expression

Mammalian

Mutation

Promoter

Available since

April 4, 2006

Availability

Academic Institutions and Nonprofits only

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pHCMV-LCMV-WE

Plasmid

#15793

Depositor

Miguel Sena-Esteves

Article

Sena-Esteves et al J Virol Methods. 2004 Dec 15. 122(2):131

Insert

Lymphocytic choriomeningitis virus glycoprotein LCMVGP gene

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 21, 2008

Availability

Academic Institutions and Nonprofits only

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pMSP1E1

Plasmid

#20062

Depositor

Stephen Sligar

Article

Denisov et al J Am Chem Soc. 2004 Mar 24. 126(11):3477

Insert

MSP1E1 (APOA1 synthetic gene)

Use

Tags

6-His

Expression

Bacterial

Mutation

"extended" MSP1; contains repeat of hel…

Promoter

Available since

Jan. 12, 2009

Availability

Academic Institutions and Nonprofits only

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pcDNA3 HA Arf6 ActQ67L

Plasmid

#10835

Depositor

Thomas Roberts

Article

Furman et al J Biol Chem. 2002 Mar 8. 277(10):7962-9.

Insert

Arf6 Q67L (ARF6 Human)

Use

Tags

HA

Expression

Mammalian

Mutation

Q67L. Defective in GTP hydrolysis. Constitutive…

Promoter

Available since

April 4, 2006

Availability

Academic Institutions and Nonprofits only

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pU6-(BbsI)_CBh-Cas9-T2A-mcherry-P2A-Ad4E4orf6

Plasmid

#64222

Purpose

Expression vector for sgRNA and for Expression of Cas9 linked via T2A to mCherry linked to the Ad4 E4orf6 gene via P2A

Depositor

Ralf Kuehn

Article

Chu et al Nat Biotechnol. 2015 Mar 24. doi: 10.103

Inserts

Cas9
sgRNA cassette

Use

CRISPR

Tags

3xFLAG, NLS, and T2A-mCherry-P2A-E4orf6

Expression

Mammalian

Mutation

Promoter

CBh and U6

Available since

May 29, 2015

Availability

Academic Institutions and Nonprofits only

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pHA-PUMA

Plasmid

#16588

Depositor

Bert Vogelstein

Article

Yu et al Mol Cell. 2001 Mar . 7(3):673-82.

Insert

PUMA (BBC3 Human)

Use

Tags

2x HA

Expression

Mammalian

Mutation

Promoter

Available since

April 24, 2008

Availability

Academic Institutions and Nonprofits only

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pCRY2FL(deltaNLS)-mCherryN1

Plasmid

#26871

Purpose

Mammalian expression of cytoplasmic CRY2-mCherry

Depositor

Chandra Tucker

Article

Kennedy et al Nat Methods. 2010 Dec;7(12):973-5. doi:

Insert

CRY2 (CRY2 Mustard Weed)

Use

Tags

mCherry

Expression

Mammalian

Mutation

NLS sequences deleted

Promoter

CMV

Available since

Feb. 24, 2011

Availability

Academic Institutions and Nonprofits only

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pNANOG-Luc

Plasmid

#25900

Depositor

Ren-he Xu

Article

Xu et al Cell Stem Cell. 2008 Aug 7. 3(2):196-206

Insert

NANOG promoter (NANOG Human)

Use

Tags

Luciferase

Expression

Mammalian

Mutation

None

Promoter

Available since

Aug. 5, 2010

Availability

Academic Institutions and Nonprofits only

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pcDNA cyclin D1 HA

Plasmid

#11181

Depositor

Bruce Zetter

Article

Newman et al J Biol Chem. 2004 Oct 1. 279(40):41504-1

Insert

cyclin D1 (CCND1 Human)

Use

Tags

HA

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 5, 2006

Availability

Academic Institutions and Nonprofits only

Enlarge