We narrowed to 32,932 results for: Eng
-
Plasmid#190794PurposeTemplate vector to amplify pieces for creating multiplex sgRNA-arrays.DepositorInsertsgRNA-scaffold only
UseSynthetic BiologyExpressionBacterialPromoterBBa_J23117Available SinceNov. 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAsCpf1(TATV)(BB) (pY221)
Plasmid#89354PurposeExpresses humanized AsCpf1 TATV PAM variant and crRNA guideDepositorInsertCpf1 RVR variant
UseCRISPRTagsNLS and NLS-3xHAExpressionMammalianMutationS542R/K548V/N552RPromoterCBhAvailable SinceJuly 12, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAC153-dual-dCas9VP96-sgExpression
Plasmid#48239PurposeDual expression construct expressing both dCas9VP96 and sgRNA from separate promotersDepositorInsertdCas9
UseCRISPRTagsHA-Tag, HA-tag, and VP96ExpressionMammalianMutationD10A H840AAvailable SinceSept. 17, 2013AvailabilityAcademic Institutions and Nonprofits only -
pYPQAT25
Plasmid#223397PurposeT-DNA vector for PmCDA1-CBE_V04 based C-to-T base editing for dicot plants; NGG PAM; 5' editing window; PmCDA1-CBE_V04 was driven by AtUBQ10 and sgRNA was driven by AtU3; Hygromycin for plants select.DepositorInsertAtUBQ10-SpCas9D10A-PmCDA-UGI-T2A-MS2-UGI-AtU3-gRNA scaffold 2.0
UseCRISPRTags3x FLAG and NLSExpressionPlantAvailable SinceApril 10, 2025AvailabilityAcademic Institutions and Nonprofits only -
EF.deltaBHES1.Ubc.GFP
Plasmid#24982DepositorInsertHES1 (HES1 Human)
UseLentiviralTagsFLAGExpressionMammalianMutationDNA-binding domain disrupted by E43A, K44A, R47A …Available SinceJune 14, 2010AvailabilityAcademic Institutions and Nonprofits only -
AAV8-cPE-C
Plasmid#183539PurposeDeliver a split compact Prime Editor in vivoDepositorInsertC-terminal fragment of SpCas9 nickase (H840A), Reverse Transcriptase
UseAAVExpressionMammalianPromoterU1AAvailable SinceJune 24, 2022AvailabilityAcademic Institutions and Nonprofits only -
dpYPQ230-D156R-CBE2
Plasmid#195368PurposedLbCas12a-D156R based cytosine base editor, hA3A/Y130F fused to the N terminal of dLbCas12a-D156R with the linker of XTENDepositorInserthA3A/Y130F-XTEN-dLbCas12a-D156R-UGI
UseCRISPR; Gateway compatible ha3a/y130f-xten-dlbcas…ExpressionPlantPromoterZmUBI1Available SinceMay 17, 2023AvailabilityAcademic Institutions and Nonprofits only -
pre_IV_CS8_7569_v1
Plasmid#126865Purpose7886 bases long scaffold with Zn-dependent self-cleaving DNAzyme cassettes, from compatibility group 8, made by method IV, yields linear 7569 b long scaffolds after Zn incubationDepositorInsertIV_CS8_7569_v1
UseSynthetic BiologyAvailable SinceJuly 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
pGL3-B-pGAS5
Plasmid#46371DepositorAvailable SinceMay 19, 2014AvailabilityAcademic Institutions and Nonprofits only -
pILGFPE9
Plasmid#83545PurposeUsed to evaluate the expression output of TEF1 promoter with yEGFP used as the reporter gene.DepositorInsertTEF1 promoter-yEGFP
ExpressionYeastPromoterTEF1Available SinceOct. 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
MMLV-RT for Split-PE (pEH45)
Plasmid#190105PurposeExpresses MMLV-RT (full-length, from PE2) for Split-PE useDepositorInsertMMLV-RT
TagsbpNLSExpressionMammalianMutationRT pentamutant from PE2 (D200N, T306K, W313F, T33…PromoterCMVAvailable SinceSept. 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
AAV8-cPE-N
Plasmid#183538PurposeDeliver a split compact Prime Editor in vivoDepositorInsertN-terminal fragment of SpCas9 nickase (H840A)
UseAAVExpressionMammalianAvailable SinceOct. 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
Ef1a_LIN28_Ires_Puro
Plasmid#18921DepositorAvailable SinceSept. 4, 2008AvailabilityAcademic Institutions and Nonprofits only -
pJT9R
Plasmid#98305PurposeOverexpressing yeast farnesyl pyrophosphate synthase Erg20p and Actinidia chinensis nerolidol/linalool synhtase AcNES1 under the control of GAL1/GAL2 promoterDepositorInsertTRPL3AcNES1>TRPL41B
ExpressionYeastAvailable SinceAug. 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
-
pEGIP*35
Plasmid#26776PurposeEnables the creation of a chromosomally integrated mutant EGFP target sequence by lentiviral transduction to assess the efficiency of HR-mediated gene targetingDepositorInsertGFP*35
UseLentiviralExpressionMammalianAvailable SinceNov. 29, 2010AvailabilityAcademic Institutions and Nonprofits only -
pCFJ150-Cas9(dpiRNA)
Plasmid#107940Purposeeft-3p::Cas9(dpiRNA)::tbb-2 3'UTR construct used for MosSCI in nematode. Cas9 is optimized by removing all piRNA targeting sites to allow germline expression.DepositorInsertCas9(dpiRNA)
ExpressionWormPromotereft-3Available SinceApril 4, 2018AvailabilityAcademic Institutions and Nonprofits only -
pFB-HTB-ASK1
Plasmid#29517DepositorInsertASK1 (SKP1 Mustard Weed)
ExpressionInsectAvailable SinceApril 14, 2011AvailabilityAcademic Institutions and Nonprofits only -
pFB-GTE-COI1
Plasmid#29516DepositorInsertCOI1 (COI1 Mustard Weed)
TagsGST and TEV cleavage siteExpressionInsectMutationNote that the first AA in TEV site is R, not EAvailable SinceMay 26, 2011AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1 HA-rnSOX9 ΔC (1-381)
Plasmid#62973PurposeExpresses HA-tagged rat SOX9 residues 1-383DepositorAvailable SinceMay 28, 2015AvailabilityAcademic Institutions and Nonprofits only -
3537 pMIG Bim
Plasmid#8786DepositorAvailable SinceApril 4, 2006AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1 Flag-Sesn2
Plasmid#61868PurposeTransient overexpression of Flag-Sesn2 (mouse)DepositorAvailable SinceJan. 20, 2015AvailabilityAcademic Institutions and Nonprofits only -
pJH4542
Plasmid#200779PurposePtwk-40s GCaMP6s T2A NLS::mNeptune unc-54 3' UTR C.elegans AVA,AVB and other neurons expression of GCaMP6 and nuclear mNeptuneDepositorInsertGCaMP6
TagsmNeptuneExpressionWormPromoterPtwk-40Available SinceMay 7, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
pET28b-LanCL2 (6xHis-LanCL2)
Plasmid#73407PurposeExpresses His tagged LanCL2DepositorAvailable SinceApril 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
dpYPQ230-D156R-ABE4
Plasmid#195370PurposedLbCas12a-D156R based adenine base editor, ecTadA8e fused to the N terminal of dLbCas12a-D156R with the linker of (GGGGS)x6DepositorInsertecTadA8e-(GGGGS)x6-dLbCas12a-D156R
UseCRISPR; Gateway compatible ectada8e-(ggggs)x6-dlb…Tags5' and 3' NLSExpressionPlantPromoterZmUBI1Available SinceMay 17, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLV-EF1a-V5-Smpd4
Plasmid#120237PurposeExpresses V5-tagged Smpd4 in lentiviral vectorDepositorAvailable SinceJan. 10, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAAV-LAP1_2-mCherry
Plasmid#159526PurposeExpresses mCherry from LAP1_2 promoterDepositorInsertmCherry
UseAAVExpressionMammalianPromoterLAP1_2Available SinceSept. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pILGFP95
Plasmid#83550PurposeUsed to evaluate the expression output of PDA1 promoter with yEGFP used as the reporter gene.DepositorInsertPDA1 promoter-yEGFP
ExpressionYeastPromoterPDA1Available SinceDec. 15, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCool-hDDB1-BPB
Plasmid#48639PurposeExpresses DDB1 BPB domain (aa 391-709) in Ecoli (ampR)DepositorInserthDDB1-BPB (DDB1 Human)
ExpressionBacterialAvailable SinceOct. 15, 2013AvailabilityAcademic Institutions and Nonprofits only -