We narrowed to 7,568 results for: Lif

1. pcDNA3.1/CALR ins5 (del198-308)-FLAG

   Plasmid

   #204540

   Purpose

   Mammalian expression of human CALR ins5 (del198-308)-FLAG

   Depositor

   Norio Komatsu

   Article

   Araki et al Blood. 2016 Jan 27. pii: blood-2015-09-6

   Insert

   CALR ins5 (del198-308)-FLAG (CALR Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   ins5 (del198-308)

   Promoter

   Available since

   Sept. 11, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA3.1/CALR ins5 (del1-197)-FLAG

   Plasmid

   #204534

   Purpose

   Mammalian expression of human CALR ins5 (del1-197)-FLAG

   Depositor

   Norio Komatsu

   Article

   Araki et al Blood. 2016 Jan 27. pii: blood-2015-09-6

   Insert

   CALR ins5 (del1-197)-FLAG (CALR Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   ins5 (del1-197)

   Promoter

   Available since

   Sept. 11, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. Lenti_EGFP-P2A-siRNAresHAUS6_IRES_Blast

   Plasmid

   #182887

   Purpose

   Transfer vector for production of lentivirus. Expresses EGFP-P2A-HAUS6 (siRNA resistant version)

   Depositor

   Daniel Gerlich

   Article

   David et al J Cell Biol. 2019 Jul 1;218(7):2150-2168

   Insert

   HAUS 6 (HAUS6 Human)
   

   Use

   Lentiviral

   Tags

   EGFP

   Expression

   Bacterial and Mammalian

   Mutation

   silet mutations in aa 197 - 203 to confer resista…

   Promoter

   EF1alpha core

   Available since

   July 27, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. Lenti_EGFP-P2A_truncHaus6_IRES_Blast

   Plasmid

   #182885

   Purpose

   Transfer vector for production of lentivirus. Control plasmid for rescue experiment of HAUS6 depletion phenotype, containing a nonsense mutation and a truncation in HAUS6.

   Depositor

   Daniel Gerlich

   Article

   David et al J Cell Biol. 2019 Jul 1;218(7):2150-2168

   Insert

   HAUS 6 (HAUS6 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Bacterial and Mammalian

   Mutation

   stop codon introduced 2 amino acids downstream of…

   Promoter

   EF1alpha core

   Available since

   July 27, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. 3` CC: Hygro – H2B-GFP-N – loxP – mScarlet

   Plasmid

   #219565

   Purpose

   3` circularization cassette to induce Cre-mediated circularization of a genomic region flanked by loxP sites with H2B-GFP reconstitution and mScarlet expression from the chromosome

   Depositor

   Andrea Ventura

   Article

   Pradella et al Nature. 2024 Dec 18. doi: 10.1038/s41586

   Insert

   HygroR_2A_H2B-GFP-N_loxP_mScarlet
   

   Use

   Unspecified

   Tags

   Expression

   Mutation

   Promoter

   EF-1a

   Available since

   Nov. 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pMSCV-hygro-STING HAQ+V155M

   Plasmid

   #102601

   Purpose

   Express STING V55M HAQ for IFN-beta Luciferase reporter assay.

   Depositor

   Nicolas Manel

   Article

   Cerboni et al J Exp Med. 2017 Jun 5;214(6):1769-1785.

   Insert

   STING (STING1 Human)
   

   Use

   Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   V155M R71H-G230A-R293Q

   Promoter

   Available since

   Jan. 22, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pYLCRISPR/Cas9P35S-N

   Plasmid

   #66191

   Purpose

   expression of Cas9 in plants, cauliflower mosaic virus 35S promoter (P35S), Neo selection

   Depositor

   Yao-Guang Liu

   Article

   Ma et al Mol Plant. 2015 Apr 24. pii: S1674-2052(

   Insert

   Cas9
   

   Use

   Tags

   Expression

   Plant

   Mutation

   plant-codon optimized with higher GC contents (62…

   Promoter

   cauliflower mosaic virus 35S promoter (P35S)

   Available since

   Oct. 23, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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8. pScalps_Puro_mTet2 catalytic domain HxD

   Plasmid

   #79611

   Purpose

   Expression of catalytically inactive mouse Tet2

   Depositor

   Silvia Monticelli

   Article

   Montagner et al Cell Rep. 2016 May 17;15(7):1566-79. doi

   Insert

   Tet2 (Tet2 Mouse)
   

   Use

   Lentiviral

   Tags

   Myc

   Expression

   Mutation

   Mutant Tet2 with H1302Y, D1304A substitutions in …

   Promoter

   Available since

   July 21, 2016

   Availability

   Academic Institutions and Nonprofits only

9. M43: pHR-SFFVp-iLID::EGFP::FTH1

   Plasmid

   #122149

   Purpose

   Self-assebled 24-mer tagged with iLID, and EGFP. Upon blue-light activation can bind up to 24 SspB-modified proteins.

   Depositor

   Clifford Brangwynne

   Article

   Bracha et al Cell. 2018 Nov 29;175(6):1467-1480.e13.

   Insert

   FTH1 (FTH1 Human)
   

   Use

   Lentiviral

   Tags

   NLS-iLID-GFP

   Expression

   Mammalian

   Mutation

   Promoter

   SFFV

   Available since

   March 22, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pYLCRISPR/Cas9P35S-H

    Plasmid

    #66189

    Purpose

    expression of Cas9 in plants, cauliflower mosaic virus 35S promoter (P35S), hygro selection

    Depositor

    Yao-Guang Liu

    Article

    Ma et al Mol Plant. 2015 Apr 24. pii: S1674-2052(

    Insert

    Cas9
    

    Use

    Tags

    Expression

    Plant

    Mutation

    plant-codon optimized with higher GC contents (62…

    Promoter

    cauliflower mosaic virus 35S promoter (P35S)

    Available since

    June 4, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pcDNA 3.1 KBTBD4 WT

    Plasmid

    #184627

    Purpose

    Mammalian expression vector with N-terminal 3xFlag for KBTBD4 WT expression in mammalian cells

    Depositor

    Vincenzo D'Angiolella

    Article

    Chen et al Cell Death Differ. 2022 Apr 4. pii: 10.1

    Insert

    N-terminal 3xFlag tagged KBTBD4 WT (KBTBD4 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    no mutation

    Promoter

    CMV

    Available since

    June 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pLenti-FRA1

    Plasmid

    #188665

    Purpose

    Expresses murine FRA1 (Fosl1)

    Depositor

    Günter Schneider

    Article

    Schneeweis et al Cell Mol Life Sci. 2022 Dec 19;80(1):12.

    Insert

    Fosl1 (Fosl1 Mouse)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hPGK

    Available since

    Dec. 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pSAM2_mCherry_Gata6

    Plasmid

    #72694

    Purpose

    Doxycyline regulated expression of Gata6 in mammalian cells

    Depositor

    Timothy Kamp

    Article

    Lalit et al Cell Stem Cell. 2016 Mar 3;18(3):354-67.

    Insert

    Gata 6 (Gata6 Mouse)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV-Doxycycline

    Available since

    Oct. 17, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. pYLCRISPR/Cas9P35S-B

    Plasmid

    #66190

    Purpose

    expression of Cas9 in plants, cauliflower mosaic virus 35S promoter (P35S), basta selection

    Depositor

    Yao-Guang Liu

    Article

    Ma et al Mol Plant. 2015 Apr 24. pii: S1674-2052(

    Insert

    Cas9
    

    Use

    Tags

    Expression

    Plant

    Mutation

    plant-codon optimized with higher GC contents (62…

    Promoter

    cauliflower mosaic virus 35S promoter (P35S)

    Available since

    Oct. 23, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCDNA5/FRT/Halo-Tag7-Geminin(1/110)_P2A_HaloTag9-Cdt(1-100)Cy+

    Plasmid

    #169337

    Purpose

    Expression of the LT-Fucci(SCA) in mammalian cells

    Depositor

    Kai Johnsson

    Article

    Frei et al Nat Methods. 2021 Dec 16. pii: 10.1038/s

    Insert

    Fucci(SCA) (GMNN, CDT1 Human)
    

    Use

    Tags

    HaloTag7-Geminin(1/110) and HaloTag9-Cdt(1-100)Cy…

    Expression

    Mammalian

    Mutation

    HaloTag9 = HaloTag7-Q165H-P174R

    Promoter

    Available since

    Nov. 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pAGT9054

    Plasmid

    #219430

    Purpose

    Transcriptional unit (MoClo-position 2): 2x35Ss_Omega enhancer_PapE-4LF2-N-SpCas9i-N_tOCS

    Depositor

    Tom Schreiber

    Article

    Schreiber et al Mol Plant. 2024 Mar 22:S1674-2052(24)000

    Insert

    LB_2x35Ss-omega enhancer:PapE-4xLF2-NLS-SpCas9i-NLS:tOCS_RB (Position 2)
    

    Use

    CRISPR; Moclo compatible level 1 module (position…

    Tags

    PapE-4LF2

    Expression

    Plant

    Mutation

    Promoter

    Available since

    July 31, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pSH1/M-FGFR1-Fv-Fvls-E

    Plasmid

    #15285

    Depositor

    David Spencer

    Article

    Freeman et al Cancer Res. 2003 Oct 1. 63(19):6237-43.

    Insert

    FGFR1 kinase, FKBP12v36 (Fgfr1 Mouse)
    

    Use

    Tags

    HA epitope and Myristoylation-targeting domain c-…

    Expression

    Mammalian

    Mutation

    FGFR1 cytoplasmic domain FKBP12 F36V

    Promoter

    Available since

    July 12, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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18. pcDNA3.3 ss-3xFLAG-hLRP5 (aa 32-1615)

    Plasmid

    #115788

    Purpose

    Expresses human LRP5 with N-terminal 3xflag tag in mammalian cells, contains artificial signal sequence upstream of tag.

    Depositor

    Harald Junge

    Article

    Lai et al Cell Rep. 2017 Jun 27;19(13):2809-2822.

    Insert

    LRP5 (LRP5 Human)
    

    Use

    Tags

    3xflag

    Expression

    Mammalian

    Mutation

    contains silent mutation that creates an internal…

    Promoter

    CMV

    Available since

    Oct. 22, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pCDNA5/FRT/HaloTag7-Geminin(1/110)_P2A_HaloTag9-Cdt(1-110)Cy-

    Plasmid

    #169338

    Purpose

    Expression of the LT-Fucci(CA) in mammalian cells

    Depositor

    Kai Johnsson

    Article

    Frei et al Nat Methods. 2021 Dec 16. pii: 10.1038/s

    Insert

    Fucci(CA) (GMNN, CDT1 Human)
    

    Use

    Tags

    HaloTag7-Geminin(1/110) and HaloTag9-Cdt(1-110)Cy…

    Expression

    Mammalian

    Mutation

    HaloTag9 = HaloTag7-Q165H-P174R

    Promoter

    Available since

    Nov. 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pcDNA3.1-3XHA-p53 Δ1-42

    Plasmid

    #196269

    Purpose

    Mammalian expression of 3xHA tagged p53 Δ1-42 (ΔTAD1)

    Depositor

    Laura Attardi

    Article

    Raj et al Mol Cell. 2022 Jul 7;82(13):2370-2384.e1

    Insert

    p53 Δ1-42 (Trp53 Mouse)
    

    Use

    Tags

    3xHA

    Expression

    Mammalian

    Mutation

    deleted amino acids 1 to 42

    Promoter

    CMV

    Available since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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21. pCW57.1 KBTBD4 WT

    Plasmid

    #184624

    Purpose

    Doxycycline inducible lentiviral vector for N-terminal Flag tagged KBTBD4 WT expression in mammalian cells

    Depositor

    Vincenzo D'Angiolella

    Article

    Chen et al Cell Death Differ. 2022 Apr 4. pii: 10.1

    Insert

    N-terminal Flag tagged KBTBD4 WT (KBTBD4 Human)
    

    Use

    Lentiviral; Doxycycline inducible

    Tags

    Expression

    Mammalian

    Mutation

    no mutation

    Promoter

    TRE promoter, Tet ON

    Available since

    June 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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22. pTRIP-SFFV-mtagBFP-2A STING V155M 368STOP

    Plasmid

    #102596

    Purpose

    Lentivector to express STING V155M delta 368 and mTagBFP

    Depositor

    Nicolas Manel

    Article

    Cerboni et al J Exp Med. 2017 Jun 5;214(6):1769-1785.

    Insert

    STING (STING1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    V155M delta 368

    Promoter

    SFFV

    Available since

    Jan. 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. FcD265A-IL2

    Plasmid

    #65419

    Purpose

    Mammalian expression plasmid for murine serum-persistent IL-2

    Depositor

    Dane Wittrup

    Article

    Zhu et al Cancer Cell. 2015 Apr 13;27(4):489-501.

    Insert

    FcD265A-IL2 (Il2 Mouse)
    

    Use

    Tags

    His

    Expression

    Mammalian

    Mutation

    D265A

    Promoter

    Modified CMV Promoter

    Available since

    June 9, 2015

    Availability

    Academic Institutions and Nonprofits only

24. Lenti-U6-sgMettl3

    Plasmid

    #196263

    Purpose

    Expresses a Mettl3-targeting sgRNA driven by the U6 promoter and Cre-recombinase driven by the PGK promoter

    Depositor

    Laura Attardi

    Article

    Raj et al Mol Cell. 2022 Jul 7;82(13):2370-2384.e1

    Insert

    Mettl3 sgRNA (Mettl3 Mouse)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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25. pOT_4 - lenti-EFS-FMC6.3-BBz-2A-puro-2A-LTBR

    Plasmid

    #181973

    Purpose

    Expresses anti-CD19 CAR (with 4-1BB signaling domain) linked to puromycin resistance via P2A and to human LTBR via T2A for lentiviral delivery.

    Depositor

    Neville Sanjana

    Article

    Legut et al Nature. 2022 Mar;603(7902):728-735. doi:

    Insert

    FMC6.3-BBz CAR; LTBR (LTBR Human, Synthetic)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pInducer-FRA1

    Plasmid

    #192267

    Purpose

    Doxycycline-inducible expression of murine FRA1 (Fosl1)

    Depositor

    Günter Schneider

    Article

    Schneeweis et al Cell Mol Life Sci. 2022 Dec 19;80(1):12.

    Insert

    Fosl1 (Fosl1 Mouse)
    

    Use

    Lentiviral; Inducible expression

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pOT_3 - lenti-EFS-FMC6.3-28z-2A-puro-2A-LTBR

    Plasmid

    #181972

    Purpose

    Expresses anti-CD19 CAR (with CD28 signaling domain) linked to puromycin resistance via P2A and to human LTBR via T2A for lentiviral delivery.

    Depositor

    Neville Sanjana

    Article

    Legut et al Nature. 2022 Mar;603(7902):728-735. doi:

    Insert

    FMC6.3-28z CAR; LTBR (LTBR Human, Synthetic)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. LZRS ERTm RasV12

    Plasmid

    #21199

    Depositor

    Paul Khavari

    Article

    Dajee et al Oncogene. 2002 Feb 28;21(10):1527-38

    Insert

    EsR1- HRas (Esr1, HRAS Mouse, Human)
    

    Use

    Retroviral

    Tags

    Expression

    Mutation

    Esr1 G257R; HRasG12V ERTm bx3

    Promoter

    Available since

    July 24, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pSH1/M-FGFR2-Fv-Fvls-E

    Plasmid

    #15286

    Depositor

    David Spencer

    Article

    Freeman et al Cancer Res. 2003 Oct 1. 63(19):6237-43.

    Insert

    FGFR2 kinase, FKBP12v36 (Fgfr2 Mouse)
    

    Use

    Tags

    Myristoylation-targeting domain c-Src (14aa) and …

    Expression

    Mammalian

    Mutation

    FGFR2 cytoplasmic domain FKBP12 F36V

    Promoter

    Available since

    July 12, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pSH1/M-FGFR3-Fv-Fvls-E

    Plasmid

    #15287

    Depositor

    David Spencer

    Article

    Freeman et al Cancer Res. 2003 Oct 1. 63(19):6237-43.

    Insert

    FGFR3 kinase, FKBP12v36 (Fgfr3 Mouse)
    

    Use

    Tags

    Myristoylation-targeting domain c-Src (14aa) and …

    Expression

    Mammalian

    Mutation

    FGFR3 cytoplasmic domain only FKBP12 F36V

    Promoter

    Available since

    July 12, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge