164,577 results

1. SP-Cas9

   Plasmid

   #62731

   Purpose

   Expresses SP-Cas9 protein in bacterial cells

   Depositor

   Niels Geijsen

   Article

   D'Astolfo et al Cell. 2015 Apr 23;161(3):674-690. doi: 1

   Insert

   SP-CAS9
   

   Use

   CRISPR

   Tags

   His tag and NLS

   Expression

   Bacterial

   Mutation

   Codon optimazed for e.coli

   Promoter

   T7

   Available Since

   April 24, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAcGFP1-N1-Dsup-deltaC

   Plasmid

   #90023

   Purpose

   To express GFP-tagged Dsup lacking C-terminal region in mammalian cells transiently

   Depositor

   Takekazu Kunieda

   Article

   Hashimoto et al Nat Commun. 2016 Sep 20;7:12808. doi: 10

   Insert

   Dsup
   

   Use

   Tags

   AcGFP1

   Expression

   Mammalian

   Mutation

   Deletion of C-terminal region

   Promoter

   CMV

   Available Since

   Aug. 11, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. pWY82

   Plasmid

   #65721

   Purpose

   Plasmid with 2.5 kb of telomere sequence

   Depositor

   James Birchler

   Article

   Yu et al Proc Natl Acad Sci U S A. 2006 Nov 14;10

   Insert

   Telomere
   

   Use

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available Since

   Oct. 30, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. pPHR-mCherry-NLS

   Plasmid

   #103819

   Purpose

   Soluble BLInCR mock effector that is recruited to 'localizer' sites upon blue light illumination

   Depositor

   Karsten Rippe

   Article

   Rademacher et al J Cell Sci. 2017 Dec 15;130(24):4213-422

   Insert

   PHR-mCherry-NLS (CRY2 Mustard Weed)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Nov. 30, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. EWS-GFP

   Plasmid

   #164701

   Purpose

   Expression of EWS-GFP

   Depositor

   Cheryl Arrowsmith

   Insert

   EWSR1 (EWSR1 Human)
   

   Use

   Tags

   AcGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Sept. 30, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pMY-MCS-P2A-PuroR-HA

   Plasmid

   #163352

   Purpose

   Retroviral backbone for in-frame cloning of a gene of interest and a P2A separated Puromycin selection marker

   Depositor

   Louis Ates, Teunis Geijtenbeek

   Article

   van der Donk et al Biol Open. 2022 Feb 15;11(2):bio059032.

   Type

   Empty backbone

   Use

   Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Jan. 15, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pGMC00018 (aka pRC0082)

   Plasmid

   #195320

   Purpose

   Minimal plasmid backbone with multiple cloning site for HDR donor DNA construction

   Depositor

   Raj Chari

   Type

   Empty backbone

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Bacterial and Mammalian

   Mutation

   Promoter

   Available Since

   March 20, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pF146 pSOD1WTAcGFP1

   Plasmid

   #26407

   Depositor

   Elizabeth Fisher

   Article

   Stevens et al PLoS One. 2010 . 5(3):e9541.

   Insert

   SOD1 (SOD1 Human)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Nov. 8, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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9. pUC19 PKC alpha

   Plasmid

   #8429

   Depositor

   Frederic Mushinski

   Insert

   Mouse Protein Kinase C alpha (Prkca Mouse)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   March 5, 2007

   Availability

   Academic Institutions and Nonprofits only

10. CL20_mEGFP_NUP98_KDM5A

    Plasmid

    #205872

    Purpose

    Express mEGFP-tagged fusion protein, NUP98_KDM5A from patient-derived sequence

    Depositor

    Richard Kriwacki

    Article

    Tripathi et al Nat Commun. 2023 Sep 28;14(1):6008. doi:

    Insert

    NUP98_KDM5A (NUP98, KDM5A Human)
    

    Use

    Tags

    mEGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Nov. 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pHCMV-VSVgSS-pAG-TM

    Plasmid

    #207318

    Purpose

    Functional incorporation of protein AG (pAG) fusion protein into envelope of virions

    Depositor

    Feng Zhang

    Article

    Strebinger et al Nat Commun. 2023 Aug 23;14(1):5141. doi:

    Insert

    protein AG
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV promoter

    Available Since

    Nov. 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. qTAG-C-dTAG-Zeo

    Plasmid

    #207738

    Purpose

    Empty C-terminus donor cassette. Integrate homology arms to target the C-terminus insertion of a dTAG-2A-Zeo cassette

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Type

    Empty backbone

    Use

    CRISPR; Donor cassette

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Nov. 15, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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13. A*68:01:01

    Plasmid

    #203292

    Purpose

    Overexpress HLA allele

    Depositor

    Catherine Wu, Derin Keskin

    Article

    Sarkizova et al Nat Biotechnol. 2020 Feb;38(2):199-209.

    Insert

    A*68:01:01 (HLA-A Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    July 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCIBN-EYFP-hPMLIII

    Plasmid

    #103808

    Purpose

    BLInCR 'Localizer' construct that marks the PML nuclear bodies and is targeted by a PHR-tagged effector upon illumination with blue light

    Depositor

    Karsten Rippe

    Article

    Rademacher et al J Cell Sci. 2017 Dec 15;130(24):4213-422

    Insert

    CIBN-EYFP-hPMLIII (CIB1, PML Human, Mustard Weed)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Jan. 9, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. Gal:mCherry-CRY2-uTEV1Δ(220-242)

    Plasmid

    #135452

    Purpose

    Yeast expression of uTEV1Δ fused to CRY2 and mCherry

    Depositor

    Alice Ting

    Article

    Sanchez et al Nat Methods. 2019 Dec 9. pii: 10.1038/s4

    Insert

    mCherry-CRY2-TEV1Δ(220-242)
    

    Use

    Tags

    mCherry

    Expression

    Yeast

    Mutation

    Promoter

    Gal

    Available Since

    Jan. 14, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. H2B-2A-GFP-IRESpuro2

    Plasmid

    #183746

    Purpose

    Expresses H2B-2A-GFP

    Depositor

    Daniel Gerlich

    Article

    Cuylen et al Nature. 2016 Jun 29;535(7611):308-12. do

    Insert

    H2B (H2BC16P Human)
    

    Use

    Tags

    GFP with 2A protease cleavage site between H2B an…

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    July 27, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCAG-eCas9-GFP-U6-gRNA

    Plasmid

    #79145

    Purpose

    All-in-one CRISPR/Cas9 vector with high-fidelity eSpCas9 expression in human pluripotent stem cells

    Depositor

    Jizhong Zou

    Insert

    eSpCas9(1.1)
    

    Use

    CRISPR

    Tags

    2A-GFP

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available Since

    June 24, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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18. ER-RA

    Plasmid

    #209872

    Purpose

    Dimerization dependent fluorescent protein RA anchored to cytosolic face of the endoplasmic reticulum membrane with N-terminal targeting sequence of rabbit CYP2C1

    Depositor

    Sarah Cohen

    Article

    Miner et al Contact (Thousand Oaks). 2024 Feb 4;7:25

    Insert

    ddRFP A
    

    Use

    Tags

    Targeting domain of CYP2C1 MDPVVVLGLCLSCLLLLSLWKQ…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Nov. 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. pIRES2-EGFP Mer

    Plasmid

    #14998

    Depositor

    Raymond Birge

    Article

    Wu et al J Cell Sci. 2005 Feb 1. 118(Pt 3):539-53

    Insert

    Mer (Mertk Mouse)
    

    Use

    Tags

    IRES EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Aug. 22, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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20. pEF1a-FB-dCas9-puro

    Plasmid

    #100547

    Purpose

    Expresses N-terminal Flag and biotin-acceptor-site (FB)-tagged dCas9 protein

    Depositor

    Jian Xu

    Article

    Liu et al Cell. 2017 Aug 24;170(5):1028-1043.e19.

    Inserts

    N-terminal Flag and biotin-acceptor-site (FB)-tagged dCas9
    puromycin
    

    Use

    CRISPR

    Tags

    FLAG, Biotin-acceptor-site (Avitag)

    Expression

    Mammalian

    Mutation

    Promoter

    Human EF1a

    Available Since

    Sept. 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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21. pAB076i6

    Plasmid

    #79200

    Purpose

    Directed Evolution of Protein-Protein Interactions

    Depositor

    David Liu

    Article

    Badran et al Nature. 2016 May 5;533(7601):58-63. doi:

    Insert

    PlacZ-opt (no operator) gIII, luxAB; Ppro1 434cI-SH2ABL1
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    PlacZ-opt (no operator); Ppro1

    Available Since

    Aug. 21, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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22. pCFJ420 - Peft-3::GFP::H2B (ubiquitous)

    Plasmid

    #34877

    Depositor

    Erik Jorgensen

    Article

    Frokjaer-Jensen et al Nat Methods. 2012 Jan 30;9(2):117-8. doi

    Insert

    Peft-3 GFP H2B tbb-2 UTR
    

    Use

    Worm targeting

    Tags

    Expression

    Mutation

    S65C

    Promoter

    eef-1A.1 (eft-3)

    Available Since

    Feb. 15, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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23. 8xNFAT-ZsG-mCD8

    Plasmid

    #162747

    Purpose

    NFAT-driven ZsGreen-1 reporter gene with mouse CD8 gene

    Depositor

    Maki Nakayama

    Article

    Landry et al Bio Protoc. 2021 Jan 20;11(2):e3883. doi

    Insert

    8x of NFAT binding motif followed by ZsGreen-1 fluorescent protein gene
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Jan. 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pNCS mHoneydew

    Plasmid

    #91760

    Purpose

    Bacterial expression of a fluorescent protein, monomeric Honeydew (mHoneydew)

    Depositor

    Erik Rodriguez, Roger Tsien

    Article

    Shaner et al Nat Biotechnol. 2004 Dec;22(12):1567-72.

    Insert

    monomeric Honeydew
    

    Use

    Tags

    no tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    June 30, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pcDNA-puro-TagBFP-β-ACTIN-3′UTR-24xMS2

    Plasmid

    #205161

    Purpose

    express β-ACTIN-3′UTR-24xMS2

    Depositor

    Weirui Ma

    Article

    Hu et al Elife. 2023 Mar 3;12:e82178. doi: 10.755

    Insert

    β-ACTIN-3′UTR-24xMS2
    

    Use

    Tags

    tagBFP

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Oct. 18, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. TMEM63A-peGFP-N1

    Plasmid

    #228590

    Purpose

    Expresses mouse TMEM63A-eGFP in mammalian cells

    Depositor

    Huanghe Yang

    Article

    Lowry et al bioRxiv [Preprint]. 2024 Sep 3:2024.02.0

    Insert

    TMEM63A/CSC1-like protein 1 isoform 1 (Tmem63a Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Dec. 2, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pLPCX-VEcadTS

    Plasmid

    #45848

    Depositor

    Martin Schwartz

    Article

    Conway et al Curr Biol. 2013 May 14. pii: S0960-9822(

    Insert

    VE-cadherin Tension Sensor (Cdh5 Mouse)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Sept. 11, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pEMS1602

    Plasmid

    #29305

    Purpose

    Pleiades (Ple) MiniPromoter expression pattern described in Portales-Casamar et al., PNAS, 2010 (PMID: 20807748) and de Leeuw et al., MTM, 2014 (PMID: 24761428).

    Depositor

    Elizabeth Simpson

    Article

    Portales-Casamar et al Proc Natl Acad Sci U S A. 2010 Sep 21. 1

    Insert

    Ple131 (MKI67 Human)
    

    Use

    Pleiades promoter project [sic, pleaides plieades]

    Tags

    Intron-LacZ-NLS

    Expression

    Mutation

    Promoter

    Available Since

    Nov. 4, 2011

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

29. miR-124-5 promoter GFP

    Plasmid

    #20792

    Depositor

    David Bartel

    Article

    Shkumatava et al Genes Dev. 2009 Feb 15. 23(4):466-81.

    Insert

    mir-124 (mir124-5 Zebrafish)
    

    Use

    Zebrafish expression

    Tags

    GFP

    Expression

    Mutation

    Promoter

    Available Since

    May 11, 2009

    Availability

    Academic Institutions and Nonprofits only

30. pDicAID_nCas9-PmCDA_NptII_Della

    Plasmid

    #91694

    Purpose

    Dicot Target-AID vector expressing dicot-optimized nCas9-PmCDA1 with sgRNA targeting SlDella

    Depositor

    Akihiko Kondo

    Article

    Shimatani et al Nat Biotechnol. 2017 Mar 27. doi: 10.103

    Insert

    SpCas9
    

    Use

    Tags

    PmCDA1

    Expression

    Plant

    Mutation

    D10A for nickase Cas9

    Promoter

    PcUbi

    Available Since

    June 6, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. hZMPSTE24-3FLAG_pQCXIP

    Plasmid

    #89759

    Purpose

    Human ZMPSTE24 mammalian expression vector. Retroviral bicistronic puromycin vector.

    Depositor

    Martin Dorf

    Article

    Fu et al J Exp Med. 2017 Feb 28. pii: jem.2016127

    Insert

    Homo sapiens zinc metallopeptidase STE24 (ZMPSTE24) (ZMPSTE24 Human)
    

    Use

    Retroviral; Bicistronic expression: puromycin re…

    Tags

    9bp spacer (NotI site) - 3xFLAG epitopes - Stop c…

    Expression

    Mammalian

    Mutation

    Y253H in ZMPSTE24 (see depositor comments below)

    Promoter

    CMV

    Available Since

    June 8, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. Calb1-T2A-dgCre targeting vector

    Plasmid

    #114438

    Purpose

    Target dgCre into the endogenous mouse Calb1 locus at the stop codon

    Depositor

    Hongkui Zeng

    Article

    Daigle et al Cell. 2018 Jul 12;174(2):465-480.e22. do

    Insert

    dgCre
    

    Use

    Mouse Targeting

    Tags

    DHFR domain and EGFP

    Expression

    Mutation

    Promoter

    none; utilizes endogenous Calb1 promoter for expr…

    Available Since

    Jan. 7, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pFUGW-FerH-ffLuc2-eGFP

    Plasmid

    #71393

    Purpose

    Lentiviral vector of luciferase-eGFP fusion gene driven by FerH promoter

    Depositor

    Glenn Merlino

    Article

    Day et al Pigment Cell Melanoma Res. 2009 Jun;22(3

    Insert

    FerH-ffLuc2-eGFP
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    FerH

    Available Since

    Dec. 9, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pLOX-TERT-iresTK

    Plasmid

    #12245

    Purpose

    2nd generation lentiviral vector for reversible immortalization of human primary cells

    Depositor

    Didier Trono

    Article

    Salmon et al Mol Ther. 2000 Oct . 2(4):404-14.

    Insert

    TERT, HSV1-TK (TERT Human)
    

    Use

    Cre/Lox and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    LoxP in 3' LTR.

    Promoter

    Available Since

    Aug. 4, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pENTR4-CLIPf (w877-2)

    Plasmid

    #29650

    Depositor

    Eric Campeau

    Type

    Empty backbone

    Use

    Entry vector

    Tags

    CLIPf

    Expression

    Mutation

    Promoter

    Available Since

    July 6, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pFC902

    Plasmid

    #106904

    Purpose

    The vector has a U3 promoter and terminator controlled gene encoding a transcript containing the sgRNA flanked by a tRNA-Gly repeat; and a gene encoding cas9 controlled by Ptef1 and Ttef1

    Depositor

    Uffe Mortensen

    Article

    Nodvig et al Fungal Genet Biol. 2018 Jan 8. pii: S108

    Inserts

    cas9
    pyrG
    U3 promoter and terminator controlled gene encoding a transcript containing the sgRNA flanked by a tRNA gly repeat
    

    Use

    CRISPR; Fungal expression

    Tags

    SV40 NLS

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    May 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pAmCyan1-C1-ADCY6

    Plasmid

    #113905

    Purpose

    N-terminal Cyan Fluorescent tag in Adenylate Cyclase 6

    Depositor

    Anjaparavanda Naren

    Article

    Thomas et al J Biol Chem. 2018 Jun 14. pii: RA118.003

    Insert

    Adenylate Cyclase 6 (ADCY6 Human)
    

    Use

    Tags

    Cyan

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    pCMV

    Available Since

    Oct. 9, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. LeGO-G2

    Plasmid

    #25917

    Depositor

    Boris Fehse

    Article

    Weber et al Mol Ther. 2008 Apr . 16(4):698-706.

    Insert

    SFFV promoter, eGFP
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    April 24, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. peGFP-HDAC1 (pc2447)

    Plasmid

    #240148

    Purpose

    Expression vector containing a fusion protein of eGFP and human HDAC1.

    Depositor

    Cristina Cardoso

    Article

    Rausch et al Nucleic Acids Res. 2020 Dec 16;48(22):12

    Insert

    HDAC1 (HDAC1 Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Sept. 2, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pFUW-TetO-Stat1

    Plasmid

    #207227

    Purpose

    doxycycline-inducible expression of Mouse Stat1in mammalian cells

    Depositor

    Filipe Pereira

    Article

    Henriques-Oliveira et al Immunity. 58, 1-20. October 2025.

    Insert

    pFUW-TetO-STAT1
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Aug. 29, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge