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We narrowed to 18,759 results for: nls

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Showing: 7441 - 7480 of 18759 results

  5. p-dCas9-p300-Hygro

    Plasmid
    #104411
    Purpose
    transient expression of dCas9-p300 fusion protein
    Depositor
    Stephan Beck
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    p300 (EP300 Human)
    Use
    CRISPR
    Expression
    Mammalian
    Promoter
    CMV promoter
    Available since
    April 2, 2020
    Availability
    Academic Institutions and Nonprofits only
    104411-plasmid-map-sequence-id-204503

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  7. eSpCas9-plus

    Plasmid
    #126767
    Purpose
    Expression plasmid for human codon-optimized increased fidelity eSpCas9-plus (w/o U6-sgRNA). Cleaves both 20nt and 5'-extended 21nt spacer containing sgRNAs with close to the same fidelity as eSpCas9.
    Depositor
    Ervin Welker
    Publication
    Kulcsar et al Nat Commun. 2020 Mar 6;11(1):1223. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    eSpCas9-plus
    Use
    CRISPR
    Tags
    3xFLAG and NLS
    Expression
    Mammalian
    Mutation
    K848A, R1060A; amino acids 1005-1013 replaced wit…
    Promoter
    CBh
    Available since
    March 6, 2020
    Availability
    Academic Institutions and Nonprofits only
    126767-plasmid-map-sequence-id-410890

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  10. YE1-BE4max-CP1028

    Plasmid
    #138160
    Purpose
    C-to-T base editor
    Depositor
    David Liu
    Publication
    Doman et al Nat Biotechnol. 2020 Feb 10. pii: 10.103
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    rAPOBEC1(YE1)-nSpCas9 (CP1028) -UGI-UGI
    Tags
    NLS
    Expression
    Mammalian
    Mutation
    Within rAPOBEC1 W90Y + R126E; within Cas9 (CP1028…
    Promoter
    CMV
    Available since
    Feb. 19, 2020
    Availability
    Academic Institutions and Nonprofits only
    138160-plasmid-map-sequence-id-272345

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  13. PX458-sgTSC2

    Plasmid
    #128098
    Purpose
    CRISPR gRNA against human TSC2 with Cas9 from S. pyogenes and 2A-EGFP
    Depositor
    Chi Van Dang
    Publication
    Walton et al Cell. 2018 Jun 28;174(1):72-87.e32. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    sgRNA against human TSC2 (TSC2 Human)
    Use
    CRISPR
    Expression
    Mammalian
    Promoter
    hU6
    Available since
    Sept. 30, 2019
    Availability
    Academic Institutions and Nonprofits only
    128098-plasmid-map-sequence-id-249673

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  15. pRS0045

    Plasmid
    #131124
    Purpose
    Mammalian Target-ACE expression vector
    Depositor
    Nozomu Yachie
    Publication
    Sakata et al Nat Biotechnol. 2020 Jun 1. pii: 10.1038
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    TadA-linker(32aa)-TadA*(7.10)-linker(32aa)-nCas9-PmCDA1-UGI
    Use
    CRISPR
    Tags
    PmCDA1, TadA, and UGI
    Expression
    Mammalian
    Mutation
    A262T, R324L, S409I, E480K, E543D, M694I, E1219V …
    Promoter
    CMV promoter
    Available since
    Sept. 23, 2019
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    131124-plasmid-map-sequence-id-257454

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  17. pPB-R1R2_EF1adCas9VP64_T2A_MS2p65HSF1-IRESbsdpA

    Plasmid
    #112924
    Purpose
    piggyBac transposon vector carrying human EF1a promoter-driven dCas9VP64-T2A-MS2p65HSF1 with Blasticidin resistant marker
    Depositor
    Kosuke Yusa
    Publication
    Chong et al Genome Biol. 2018 Nov 26;19(1):205. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    EF1a promoter, dCas9VP94, MS2p65HSF1, IRESneo
    Use
    Piggybac transposon vector
    Expression
    Mammalian
    Available since
    Sept. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    112924-plasmid-map-sequence-id-217554

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  19. pGL3-sgRab18.N-Cas9-T2A-mCherry-P2A-Puro

    Plasmid
    #129418
    Purpose
    Encoding Cas9 and sgRAB18.N for CRISPR/Cas9 mediated HDR tagging of endogenous human RAB18 N-terminus
    Depositor
    Elina Ikonen
    Publication
    Salo et al Dev Cell. 2019 Jun 6. pii: S1534-5807(19
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    SpCas9 and sgRAB18.N (RAB18 Human)
    Use
    CRISPR
    Tags
    T2A-mCherry-P2A-Puro
    Expression
    Mammalian
    Promoter
    hU6
    Available since
    Aug. 22, 2019
    Availability
    Academic Institutions and Nonprofits only
    129418-plasmid-map-sequence-id-254820

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  20. pMGS55 (GFP-ARF16-PB1-MCS-P2A-OsTIR1)

    Plasmid
    #129667
    Purpose
    A repair construct to express GFP-ARF16-PB1-MCS and OsTIR1 under the control of the CMV promoter from the human AAVS1 locus
    Depositor
    Michael Guertin
    Publication
    Sathyan et al Genes Dev. 2019 Oct 1;33(19-20):1441-145
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    OsARF16-PB1 domain
    Tags
    GFP
    Expression
    Mammalian
    Available since
    Aug. 15, 2019
    Availability
    Academic Institutions and Nonprofits only
    129667-plasmid-map-sequence-id-254332

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  23. CAG-ABEmax-P2A-EGFP-ires-puro

    Plasmid
    #121170
    Purpose
    CAG promoter driven expression of ABEmax base editor, EGFP and Puromycin resistance.
    Depositor
    Timo Otonkoski
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    ABEmax-P2A-EGFP-ires-puro
    Use
    CRISPR
    Tags
    P2A-EGFP-ires-puro
    Expression
    Mammalian
    Available since
    July 18, 2019
    Availability
    Academic Institutions and Nonprofits only
    121170-plasmid-map-sequence-id-234985

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  24. pCAGGS-pac-T2A-iCre (v2)

    Plasmid
    #125821
    Purpose
    Puromycin resistance gene and iCre recombinase (version 2)
    Depositor
    Mario Capecchi
    Insert
    pac-T2A-iCre
    Use
    Cre/Lox
    Expression
    Mammalian
    Available since
    June 19, 2019
    Availability
    Academic Institutions and Nonprofits only
    125821-plasmid-map-sequence-id-247197

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  25. pCAGGS-pac-T2A-dre

    Plasmid
    #124833
    Purpose
    Puromycin resistance gene and dre recombinase
    Depositor
    Mario Capecchi
    Insert
    pac-T2A-dre
    Expression
    Mammalian
    Promoter
    CAG
    Available since
    June 19, 2019
    Availability
    Academic Institutions and Nonprofits only
    124833-plasmid-map-sequence-id-247139

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  28. hGli3 NTOTAL in AINGpCAGGS

    Plasmid
    #121973
    Purpose
    medium Gli3 transcriptional repressor
    Depositor
    James Briscoe
    Publication
    Ulloa et al Curr Biol. 2007 Mar 20;17(6):545-50. doi
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    hGli3 NTOTAL (GLI3 Human)
    Use
    Chick electroporation
    Tags
    5xmyc and Linker A IRES NLS GFP pCAGGS
    Expression
    Mammalian
    Mutation
    C terminus deletion from zinc finger domain
    Available since
    April 23, 2019
    Availability
    Academic Institutions and Nonprofits only
    121973-plasmid-map-sequence-id-241936

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  29. pLV hUbC- VP64-dCas9-VP64-T2A- GFP(No LoxP sites)

    Plasmid
    #66708
    Purpose
    expresses VP64-dCas9-VP64, same plasmid as Addgene 59791 but with LoxP sites removed
    Depositor
    Charles Gersbach
    Insert
    S.Pyogenes VP64-dCas9-VP64
    Use
    Lentiviral and Synthetic Biology
    Tags
    VP64
    Mutation
    D10A and H840A
    Promoter
    hUbC
    Available since
    April 12, 2019
    Availability
    Academic Institutions and Nonprofits only
    66708-plasmid-map-sequence-id-449286

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  30. Lenti-(BB)-EF1a-KRAB-dCas9-P2A-BlastR EGFP-guide1

    Plasmid
    #118158
    Purpose
    CRISPRi negative control. Catalytically inactive Cas9 from S. pyogenes with 2A-BlastR under the EF1a core promoter, and sgRNA1 against the EGFP CDS
    Depositor
    Jorge Ferrer
    Insert
    KRAB-dCas9-2A-BlastR
    Use
    CRISPR and Lentiviral
    Tags
    HA
    Expression
    Mammalian
    Promoter
    EF1a core and U6
    Available since
    April 2, 2019
    Availability
    Academic Institutions and Nonprofits only
    118158-plasmid-map-sequence-id-240404

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  32. pCAGG-NFIC2

    Plasmid
    #112701
    Purpose
    Expression of NFIC2 protein and GFP
    Depositor
    Richard Gronostajski
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Nuclear Factor I C2 (Nfic Mouse)
    Tags
    GFP and HA
    Expression
    Mammalian
    Available since
    Feb. 20, 2019
    Availability
    Academic Institutions and Nonprofits only
    112701-plasmid-map-sequence-id-212389

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  33. pOTTC763 - pX458 with rat Rosa26 gRNA A

    Plasmid
    #113161
    Purpose
    A plasmid that expresses a guide RNA targeting rat Rosa26 as well as FLAG-tagged SpCas9 and GFP
    Depositor
    Brandon Harvey
    Publication
    Back et al Neuron. 2019 Feb 8. pii: S0896-6273(19)3
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    gRNA for rat Rosa26
    Use
    CRISPR
    Expression
    Mammalian
    Promoter
    hU6
    Available since
    Feb. 19, 2019
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    113161-plasmid-map-sequence-id-236005

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  36. pAAV-CMV-CASANOVA

    Plasmid
    #113036
    Purpose
    AAV vector for CASANOVA (AcrIIA4-LOV2 hybrid) expression in mammalian cells; enables optogenetic control of SpyCas9
    Depositor
    Dominik Niopek
    Publication
    Bubeck et al Nat Methods. 2018 Oct 30. pii: 10.1038/s
    Insert
    CASANOVA (for CRISPR/Cas9 activity switching via a novel optogenetic variant of AcrIIA4)
    Use
    AAV, CRISPR, and Synthetic Biology
    Expression
    Mammalian
    Available since
    Nov. 1, 2018
    Availability
    Academic Institutions and Nonprofits only
    113036-plasmid-map-sequence-id-226953

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Showing: 7441 - 7480 of 18759 results