59,538 results

1. VPS35 shRNA2

   Plasmid

   #163859

   Purpose

   shRNA targeting the coding sequences of VPS35

   Depositor

   Lei Lu

   Article

   Sun et al J Cell Sci. 2021 Oct 15;134(20). pii: 27

   Insert

   VPS35 retromer complex component (VPS35 Human)
   

   Expression

   Mammalian

   Available Since

   Nov. 24, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. GPNMBA-c200

   Plasmid

   #205111

   Purpose

   GPNMB protein expression

   Depositor

   Paul Brennan

   Insert

   GPNMB (GPNMB Human)
   

   Tags

   AviTag-6His and PTPRSsp

   Expression

   Mammalian

   Mutation

   WT

   Available Since

   Dec. 22, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pDONR223-GCK

   Plasmid

   #23750

   Depositor

   William Hahn, David Root

   Article

   Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

   Insert

   GCK (GCK Human)
   

   Use

   Gateway donor vector

   Available Since

   July 30, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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4. pRK5-HA-Ubiquitin-K6

   Plasmid

   #121151

   Purpose

   Mammalian expression of HA tagged ubiquitin with K6 only, other lysines mutated to arginines

   Depositor

   Josef Kittler

   Article

   Birsa et al J Biol Chem. 2014 May 23;289(21):14569-8

   Insert

   Ubiquitin C (UBC Human)
   

   Expression

   Mammalian

   Mutation

   All Ks apart from K6 mutated to Rs

   Available Since

   Feb. 19, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. Gi1-CASE

   Plasmid

   #168120

   Purpose

   Encodes a BRET-based activity sensor for the heterotrimeric G protein Gi1. Composed of the subunits G alpha i1 (GNAI1) tagged with NanoLuciferase, G beta 1 (GNB1) and Venus-tagged G gamma 2 (GNG2).

   Depositor

   Gunnar Schulte

   Article

   Schihada et al Sci Signal. 2021 Sep 7;14(699):eabf1653.

   Insert

   GNAI1, GNB1, GNG2 (GNB1, GNG2, GNAI1 Human)
   

   Use

   Luciferase

   Tags

   cpVenus on GNG2

   Expression

   Mammalian

   Mutation

   NLuc is inserted at A121/E122 within GNAI1

   Available Since

   July 29, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. B1-INTEGRIN WT

   Plasmid

   #195215

   Purpose

   Mammalian expression of mCherry tagged B1 Integrin. Wild-type.

   Depositor

   David Bryant, Valerie Weaver

   Article

   Paszek et al Cancer Cell. 2005 Sep;8(3):241-54. doi:

   Insert

   ITGB1 (ITGB1 Human)
   

   Expression

   Mammalian

   Available Since

   March 13, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pDONR223_STAT3_p.A662C_N664C

   Plasmid

   #82184

   Purpose

   Gateway Donor vector containing STAT3 , part of the Target Accelerator Plasmid Collection.

   Depositor

   Jesse Boehm, William Hahn, David Root

   Article

   Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

   Insert

   STAT3 (STAT3 Human)
   

   Use

   Gateway entry vector

   Mutation

   A662C, N664C

   Promoter

   None

   Available Since

   April 24, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. pDONR223_FGF1_WT

   Plasmid

   #81825

   Purpose

   Gateway Donor vector containing FGF1 , part of the Target Accelerator Plasmid Collection.

   Depositor

   Jesse Boehm, William Hahn, David Root

   Article

   Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

   Insert

   FGF1 (FGF1 Human)
   

   Use

   Gateway entry vector

   Mutation

   WT

   Promoter

   None

   Available Since

   April 24, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. pWPI-LUC7L2-3xFLAG

   Plasmid

   #201641

   Purpose

   Over-expression of 3xFLAG-tagged human LUC7L2

   Depositor

   Alexis Jourdain, Vamsi Mootha

   Article

   Jourdain et al Mol Cell. 2021 May 6;81(9):1905-1919.e12

   Insert

   LUC7L2 (LUC7L2 Human)
   

   Use

   Lentiviral

   Tags

   3xFLAG

   Available Since

   Aug. 9, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pPHR-mCherry-hNPM

    Plasmid

    #181887

    Purpose

    Soluble light-dependent effector protein (nucleophosmin) for optodroplet formation or recruitment to CIBN localizers

    Depositor

    Karsten Rippe

    Article

    Erdel et al Mol Cell. 2020 Apr 16;78(2):236-249.e7.

    Insert

    PHR-mCherry-hNPM (NPM1 Mustard Weed, Human)
    

    Tags

    PHR-mCherry

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    April 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. UbcH5c WT pET28a

    Plasmid

    #12643

    Depositor

    Rachel Klevit

    Article

    Brzovic et al Mol Cell. 2006 Mar 17. 21(6):873-80.

    Insert

    UbcH5c - Ubiquitin conjugating enzyme (UBE2D3 Human)
    

    Expression

    Bacterial

    Available Since

    July 31, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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12. pHAGE-PIK3CA

    Plasmid

    #116771

    Purpose

    Lentiviral expression of PIK3CA

    Depositor

    Gordon Mills, Kenneth Scott

    Article

    Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

    Insert

    PIK3CA (PIK3CA Human)
    

    Use

    Lentiviral

    Mutation

    WT

    Available Since

    March 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. GRB2-V5

    Plasmid

    #139631

    Purpose

    V5-His-tagged gene expression

    Depositor

    Markku Varjosalo

    Article

    Yadav et al iScience. 2020 Feb 21;23(2):100871. doi:

    Insert

    GRB2 (GRB2 Human)
    

    Tags

    3xV5-6xHis

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    March 24, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. SYNJ1_BirA_Nterm

    Plasmid

    #221523

    Purpose

    Flag-tagged biotinylation enzyme for co-expression of Synj1

    Depositor

    Alessandro Ori

    Insert

    SYNJ1 (SYNJ1 Human)
    

    Tags

    BirA

    Expression

    Mammalian

    Available Since

    July 16, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pcDNA-HA-ER D538G

    Plasmid

    #49500

    Purpose

    Expresses HA-tagged ER D538G mutant in mammalian cells

    Depositor

    Sarat Chandarlapaty

    Article

    Toy et al Nat Genet. 2013 Dec;45(12):1439-45. doi:

    Insert

    ESR1 (ESR1 Human)
    

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Aspartic acid 538 to Glycine

    Promoter

    CMV

    Available Since

    Dec. 12, 2013

    Availability

    Academic Institutions and Nonprofits only

16. TMED2-bio-His

    Plasmid

    #51853

    Purpose

    Expresses full-length Transmembrane emp24 domain-containing protein 2 precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.

    Depositor

    Gavin Wright

    Article

    Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m

    Insert

    TMED2 (TMED2 Human)
    

    Tags

    His tag, enzymatic biotinylation sequence, and ra…

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 27, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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17. pEGFPC1-EPSIN 2

    Plasmid

    #22242

    Depositor

    Pietro De Camilli

    Article

    Rosenthal et al J Biol Chem. 1999 Nov 26. 274(48):33959-

    Insert

    Epsin 2 (EPN2 Human)
    

    Tags

    GFP

    Expression

    Bacterial and Mammalian

    Available Since

    Dec. 3, 2009

    Availability

    Academic Institutions and Nonprofits only

18. Profiline

    Plasmid

    #89925

    Purpose

    Binds to actin and affects the structure of the cytoskeleton. At high concentrations, profilin prevents the polymerization of actin

    Depositor

    Antoine Jégou, Guillaume Romet-Lemonne

    Article

    Jegou et al PLoS Biol. 2011 Sep;9(9):e1001161. doi:

    Insert

    profiline-1 (PFN1 Human)
    

    Tags

    None

    Expression

    Bacterial

    Available Since

    July 20, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. PB-SAM

    Plasmid

    #102559

    Purpose

    Piggybac transposon vector encoding dCAS9-VP64 and MS2-P65-HSF1 activator helper complex.

    Depositor

    Ying Liu

    Article

    Li et al Mol Ther Nucleic Acids. 2017 Sep 15;8:64

    Inserts

    MS2-P65-HSF1_T2A_Hygro
    dCAS9(D10A, N863A)-VP64_T2A_Blast
    

    Use

    CRISPR; Piggybac transposon

    Expression

    Mammalian

    Mutation

    D10A and N863A in Cas9 and N55K in MS2

    Promoter

    CAG and EF1A

    Available Since

    Jan. 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. FLAG.PKCepsilon.K/W

    Plasmid

    #10796

    Depositor

    Alex Toker

    Article

    Cenni et al Biochem J. 2002 May 1. 363(Pt 3):537-45.

    Insert

    PKC epsilon (PRKCE Human)
    

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Kinase dead mutant: K437W (Lys 437 to Trp)

    Available Since

    July 18, 2006

    Availability

    Academic Institutions and Nonprofits only