We narrowed to 9,644 results for: SUB

1. EGFP–COSMO–PB

   Plasmid

   #169684

   Purpose

   COSMO responds to caffeine to induce dimerization of a phospholipid-binding polybasic domain with subsequent plasma membrane translocation.

   Depositor

   Yubin Zhou

   Article

   Wang et al Adv Sci (Weinh). 2020 Dec 13;8(3):200214

   Insert

   anti-caffeine nanobody variant
   

   Tags

   modified STIM1–PB tag

   Expression

   Mammalian

   Mutation

   Y104W

   Promoter

   CMV

   Available Since

   July 21, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. N1-mSin1.5-GFP

   Plasmid

   #72909

   Purpose

   GFP-tagged mSin1 (isoform 5)

   Depositor

   Ivan Yudushkin

   Article

   Ebner et al J Cell Biol. 2017 Jan 31. pii: jcb.20161

   Insert

   Target of rapamycin complex 2 subunit MAPKAP1, isoform 5 (MAPKAP1 Human)
   

   Tags

   eGFP

   Expression

   Mammalian

   Available Since

   March 21, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. actc1b-mKate2-2xFYVEhgs

   Plasmid

   #109505

   Purpose

   Muscle specific zebrafish expression of mKate2 fused to mouse 2x PH domain from HGF regulated tyrosine kinase substrate (Hgs). Binds PtdIns3P

   Depositor

   Rob Parton

   Article

   Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

   Insert

   actc1b-mKate2-2xFYVEhgs
   

   Use

   Zebrafish plasmids

   Available Since

   Feb. 27, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. p231-pOsAct1::hpt-pZmUbi::BdPetC-p35S::mTurquoise

   Plasmid

   #129650

   Purpose

   Rieske FeS overexpression

   Depositor

   Susanne von Caemmerer

   Article

   Ermakova et al Commun Biol. 2019 Aug 16;2:314. doi: 10.

   Insert

   CDS of PetC gene encoding for the Rieske FeS subunit
   

   Expression

   Plant

   Mutation

   Codon modified for Golden Gate

   Promoter

   pZmUbi

   Available Since

   Oct. 30, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA3.1(+)-ExRai-AKAR(T/A)

   Plasmid

   #118406

   Purpose

   Negative-control mutant for ExRai-AKAR biosensor.

   Depositor

   Jin Zhang

   Article

   Mehta et al Nat Cell Biol. 2018 Sep 24. pii: 10.1038

   Insert

   ExRai-AKAR(T/A)
   

   Tags

   6xHis, T7 tag (gene 10 leader), and Xpress (TM) t…

   Expression

   Mammalian

   Mutation

   Contains Thr-to-Ala mutation in substrate sequenc…

   Promoter

   CMV

   Available Since

   April 25, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pBEST_OR2-OR1-Pr-UTR1_ClpP-T500

   Plasmid

   #153302

   Purpose

   Expresses ClpP protease in the cell-free TX-TL system

   Depositor

   Mikhail Shapiro

   Article

   Lakshmanan et al Nat Chem Biol. 2020 Sep;16(9):988-996. d

   Insert

   clpP (clpP Escherichia coli)
   

   Expression

   Bacterial

   Available Since

   Sept. 21, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pDONR221_SLC22A3

   Plasmid

   #132024

   Purpose

   Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

   Depositor

   Giulio Superti-Furga, RESOLUTE Consortium

   Article

   Mocking et al Int J Mol Sci. 2022 Jan 21;23(3):1203. d

   Insert

   SLC22A3 (SLC22A3 Human)
   

   Expression

   Mammalian

   Available Since

   Oct. 25, 2019

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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8. pcDNA3.1(+)-sapphireAKAR(T/A)

   Plasmid

   #118465

   Purpose

   Negative-control mutant for sapphireAKAR biosensor.

   Depositor

   Jin Zhang

   Article

   Mehta et al Nat Cell Biol. 2018 Sep 24. pii: 10.1038

   Insert

   sapphireAKAR(T/A)
   

   Tags

   6xHis, T7 tag (gene 10 leader), and Xpress (TM) t…

   Expression

   Mammalian

   Mutation

   Contains Thr-to-Ala mutation in substrate sequenc…

   Promoter

   CMV

   Available Since

   Dec. 6, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCS2+MT-hToca-1-MGD

   Plasmid

   #33033

   Depositor

   Marc Kirschner

   Article

   Ho et al Cell. 2004 Jul 23;118(2):203-16.

   Insert

   Toca-1 (FNBP1L Human)
   

   Tags

   6xMyc

   Expression

   Mammalian

   Mutation

   MGD = amino acid substitutions M383I, G384S and D…

   Promoter

   simian CMV IE94

   Available Since

   Nov. 18, 2011

   Availability

   Academic Institutions and Nonprofits only

10. mGFAP-Halo

    Plasmid

    #169474

    Purpose

    the host vector was obtained by substitution of the EGFP tag from a pEGFP-N3-mGFAP plasmid for a HaloTag using BamHI and NotI restriction sites

    Depositor

    Federico Herrera

    Article

    Letra-Vilela et al Experimental Results (2020), Volume 1, e

    Insert

    GFAP (Gfap Mouse)
    

    Tags

    HaloTag

    Expression

    Mammalian

    Mutation

    Changed R 236 to H

    Available Since

    June 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pRKB134_GI-DUX4-intron2(-)

    Plasmid

    #83968

    Purpose

    to test DUX4 mRNA is subjected to NMD upon intron2 inclusion

    Depositor

    Robert Bradley

    Article

    Feng et al Elife. 2015 Jan 7;4. doi: 10.7554/eLife.

    Insert

    DUX4-3'UTR-intron2(-) (DUX4 Human)
    

    Expression

    Mammalian

    Promoter

    mouseCMV

    Available Since

    Jan. 17, 2017

    Availability

    Academic Institutions and Nonprofits only

12. pRKB133_GI-DUX4-intron2(+)

    Plasmid

    #83967

    Purpose

    to test DUX4 mRNA is subjected to NMD upon intron2 inclusion

    Depositor

    Robert Bradley

    Article

    Feng et al Elife. 2015 Jan 7;4. doi: 10.7554/eLife.

    Insert

    DUX4-3'UTR-intron2(+) (DUX4 Human)
    

    Expression

    Mammalian

    Promoter

    mouseCMV

    Available Since

    Nov. 3, 2016

    Availability

    Academic Institutions and Nonprofits only

13. pSG5-Cyp26a1

    Plasmid

    #13457

    Depositor

    T. Michael Underhill

    Article

    Hoffman et al J Cell Biol. 2006 Jul 3. 174(1):101-13.

    Insert

    Cyp26a1 (Cyp26a1 Mouse)
    

    Expression

    Mammalian

    Available Since

    Nov. 17, 2006

    Availability

    Academic Institutions and Nonprofits only

14. pFX-mito-MiCy

    Plasmid

    #174177

    Purpose

    Express MiCy with mitochondria-targeting sequence in mammalian cells

    Depositor

    Yusuke Ohba

    Article

    Kashiwagi et al Cell Struct Funct. 2019 Dec 26;44(2):183

    Insert

    MiCy with mitochondria-targeting sequence
    

    Tags

    the mitochondria-targeting sequence of the cytoch…

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 18, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pcDNA3-RAB-AKARev(T/A)

    Plasmid

    #118479

    Purpose

    Negative-control mutant for RAB-AKARev biosensor.

    Depositor

    Jin Zhang

    Article

    Mehta et al Nat Cell Biol. 2018 Sep 24. pii: 10.1038

    Insert

    RAB-AKARev(T/A)
    

    Tags

    6xHIS, T7 tag (gene 10 leader), and Xpress (TM) t…

    Expression

    Mammalian

    Mutation

    Contains Thr-to-Ala mutation in substrate sequenc…

    Promoter

    CMV

    Available Since

    Dec. 21, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pcDNA3-blueCKAR(T/A)

    Plasmid

    #118473

    Purpose

    Negative-control mutant for blueCKAR biosensor.

    Depositor

    Jin Zhang

    Article

    Mehta et al Nat Cell Biol. 2018 Sep 24. pii: 10.1038

    Insert

    blueCKAR(T/A)
    

    Tags

    6xHIS, T7 tag (gene 10 leader), and Xpress (TM) t…

    Expression

    Mammalian

    Mutation

    Contains Thr-to-Ala mutation in substrate sequenc…

    Promoter

    CMV

    Available Since

    Dec. 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. pcDNA3-JNKAR1-NES

    Plasmid

    #84632

    Purpose

    Unimolecular, FRET-based JNK activity reporter; cytosol targeted

    Depositor

    Jin Zhang

    Article

    Depry et al Chem Biol. 2015 Nov 19;22(11):1470-9. do

    Insert

    ECFP-FHA1-JNKsubDD-Citrine-NES
    

    Tags

    6xHis, Citrine, ECFP, Nuclear export signal (NES)…

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Nov. 14, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pDONR221-rap1-K9R

    Plasmid

    #84527

    Purpose

    This plasmid can be used as a donor for subcloning into Gateway destination vectors.

    Depositor

    Jorrit Enserink

    Article

    Chymkowitch et al Genome Res. 2015 Jun;25(6):897-906. doi:

    Insert

    RAP1 (RAP1 Budding Yeast)
    

    Use

    Gateway donor plasmid

    Mutation

    K(43,61,69,117,240,246,527,582,651)R

    Promoter

    none

    Available Since

    Jan. 17, 2017

    Availability

    Academic Institutions and Nonprofits only

19. pLXIN-Ap1g1-FKBP

    Plasmid

    #46946

    Purpose

    Retroviral plasmid for co-expressing siRNA-resistant, FKBP-tagged AP-1 gamma subunit and G418 resistance gene in mammalian cells

    Depositor

    Margaret Robinson

    Article

    Robinson et al Dev Cell. 2010 Feb 16;18(2):324-31. doi:

    Insert

    Ap1g1-FKBP
    

    Use

    Retroviral

    Tags

    Mainly mouse Ap1g1 cDNA, with a fragment of brain…

    Expression

    Mammalian

    Available Since

    Dec. 27, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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20. pFX-Venus-Sec61β

    Plasmid

    #174467

    Purpose

    Express Venus with endoplasmic reticulum-targeting sequence in mammalian cells

    Depositor

    Yusuke Ohba

    Article

    Kashiwagi et al Cell Struct Funct. 2019 Dec 26;44(2):183

    Insert

    Venus with endoplasmic reticulum-targeting sequence
    

    Tags

    Sec61 subunit beta

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 18, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. aL-mEos3.2

    Plasmid

    #113154

    Purpose

    constrained LFA-1-mEos3.2 fusion

    Depositor

    Timothy Springer

    Article

    Moore et al Cell Rep. 2018 Feb 13;22(7):1903-1912. d

    Insert

    full-length human aL with mEOS3.2 in betapropeller (ITGAL Human)
    

    Tags

    mEOS3.2

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Oct. 16, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. delta-EGFP

    Plasmid

    #119223

    Purpose

    GABAA receptor expression (delta subunit with EGFP tag at the C-terminus)

    Depositor

    Angel de Blas

    Article

    Christie et al Mol Cell Neurosci. 2006 Jan;31(1):1-14.

    Insert

    GABRD (Gabrd Rat)
    

    Tags

    eGFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Dec. 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pLenti-EF1a-MTS-FLAG-LOV*

    Plasmid

    #202208

    Purpose

    Plasmid for the generation of a lentiviral vector encoding the photoproximity labeling catalyst LOV* fused to the mitochondria targeting sequence (MTS) in mammalian cells

    Depositor

    Thomas Muir

    Article

    Hananya et al Proc Natl Acad Sci U S A. 2023 Apr 18;12

    Insert

    Cytochrome c oxidase subunit 4 isoform 1, mitochondrial, aa 1-24
    

    Use

    Lentiviral

    Tags

    FLAG and LOV*

    Promoter

    Ef1a

    Available Since

    Aug. 1, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pcDNA3.1V5-His Snk/Plk2 D223N

    Plasmid

    #22218

    Depositor

    Wafik El-Deiry

    Article

    Burns et al Mol Cell Biol. 2003 Aug . 23(16):5556-71

    Insert

    Snk/Plk2 (PLK2 Human)
    

    Expression

    Mammalian

    Mutation

    single amino acid substitution mutant, aspartic a…

    Available Since

    Aug. 27, 2012

    Availability

    Academic Institutions and Nonprofits only

25. pFX-mCherry-Sec61β

    Plasmid

    #174468

    Purpose

    Express mCherry with endoplasmic reticulum-targeting sequence in mammalian cells

    Depositor

    Yusuke Ohba

    Article

    Kashiwagi et al Cell Struct Funct. 2019 Dec 26;44(2):183

    Insert

    Venus with endoplasmic reticulum-targeting sequence
    

    Tags

    Sec61 subunit beta

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 18, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pEGFP eIF2beta

    Plasmid

    #45902

    Depositor

    Louise Larose

    Article

    Kebache et al Proc Natl Acad Sci U S A. 2002 Apr 16;99

    Insert

    eIF2S2 (Eif2s2 Mouse)
    

    Tags

    EGFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 29, 2014

    Availability

    Academic Institutions and Nonprofits only

27. D44_Ld_180090

    Plasmid

    #135858

    Purpose

    Expression of Leishmania donovani ectodomain in mammalian cells

    Depositor

    Gavin Wright

    Article

    Roberts et al PLoS Pathog. 2022 Feb 24;18(2):e1010364.

    Insert

    alpha glucosidase II subunit, putative
    

    Tags

    biotinylation sequence and His tag

    Expression

    Mammalian

    Available Since

    March 12, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. CTA2-mNG211

    Plasmid

    #141167

    Purpose

    Expresses GST-SUMO tagged CTA2 Subunit with 11th Strand

    Depositor

    Wayne Lencer

    Article

    Luong et al Mol Biol Cell. 2019 Nov 27:mbcE19070375.

    Insert

    SUMO-CTA2-mNG211
    

    Tags

    SUMO

    Expression

    Bacterial

    Available Since

    July 31, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pAimR

    Plasmid

    #98687

    Purpose

    Expresses AimR gene from the B. subtilis phage phi3T, with its native promoter, C' His-tag

    Depositor

    Rotem Sorek

    Article

    Erez et al Nature. 2017 Jan 26;541(7638):488-493. d

    Insert

    AimR
    

    Tags

    His-tag

    Expression

    Bacterial

    Promoter

    Native

    Available Since

    Sept. 6, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pBAD-SPW

    Plasmid

    #190628

    Purpose

    Bacterial expression of genetically encoded protease indicator SPW

    Depositor

    Robert Campbell

    Article

    Gohil et al ACS Sens. 2023 Feb 24;8(2):587-597. doi:

    Insert

    SPW
    

    Expression

    Bacterial

    Available Since

    Nov. 23, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pCAG-Gdf7

    Plasmid

    #163597

    Purpose

    Expresses GDF-7 in mammalian cells

    Depositor

    Takeshi Imai

    Article

    Aihara et al Cell Rep. 2021 Jun 22;35(12):109276. doi

    Insert

    Gdf7 (Gdf7 Mouse)
    

    Expression

    Mammalian

    Mutation

    Some Guanin and Cytosine were substituted to redu…

    Available Since

    Aug. 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pCellFree_G03 CTTN

    Plasmid

    #67100

    Purpose

    Standard for cell-free expression benchmarking.

    Depositor

    Kirill Alexandrov

    Article

    Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

    Insert

    CTTN (CTTN Human)
    

    Use

    Cell-free expression

    Tags

    EGFP and His

    Promoter

    T7

    Available Since

    Nov. 6, 2015

    Availability

    Academic Institutions and Nonprofits only

33. pJMP1273

    Plasmid

    #119265

    Purpose

    E. coli attTn7 site with B. subtilis flanking sequence cloned into pACYC

    Depositor

    Jason Peters, Carol Gross, Oren Rosenberg

    Article

    Peters et al Nat Microbiol. 2019 Jan 7. pii: 10.1038/

    Insert

    E. coli attTn7
    

    Expression

    Bacterial

    Available Since

    Jan. 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. p15aC-3D1D-5

    Plasmid

    #121050

    Purpose

    Bacterial expression of PIS, PI4K and PI3P5K mutant E545K

    Depositor

    Sanford Simon

    Article

    Botero et al Sci Adv. 2019 Mar 27;5(3):eaat4872. doi:

    Inserts

    PIS
    phosphatidylinositol 4-phosphate 5-kinase
    phosphatidylinositol 3-kinase catalytic subunit p110α of class IA E545K
    

    Tags

    myc and none

    Expression

    Bacterial

    Available Since

    April 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pCNL-N1_Mito

    Plasmid

    #65670

    Purpose

    Expresses Mito-CNL in mammalian cells

    Depositor

    Yasushi Okada

    Article

    Takai et al Proc Natl Acad Sci U S A. 2015 Apr 7;112

    Insert

    cytochrome c oxidase subunit VIII
    

    Use

    Luciferase

    Tags

    CNL

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    June 11, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pCellFree_G03 CSNK2A1

    Plasmid

    #67085

    Purpose

    Standard for cell-free expression benchmarking.

    Depositor

    Kirill Alexandrov

    Article

    Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

    Insert

    CSNK2A1 (CSNK2A1 Human)
    

    Use

    Cell-free expression

    Tags

    EGFP and His

    Promoter

    T7

    Available Since

    Nov. 6, 2015

    Availability

    Academic Institutions and Nonprofits only

37. GST eIF2beta

    Plasmid

    #45900

    Depositor

    Louise Larose

    Article

    Kebache et al Proc Natl Acad Sci U S A. 2002 Apr 16;99

    Insert

    eIF2S2 (Eif2s2 Mouse)
    

    Tags

    GST

    Expression

    Bacterial

    Promoter

    tac

    Available Since

    June 16, 2014

    Availability

    Academic Institutions and Nonprofits only

38. pDONR221-RAP1

    Plasmid

    #84525

    Purpose

    This plasmid can be used as a donor for subcloning into Gateway destination vectors.

    Depositor

    Jorrit Enserink

    Article

    Chymkowitch et al Genome Res. 2015 Jun;25(6):897-906. doi:

    Insert

    RAP1 (RAP1 Budding Yeast)
    

    Use

    Gateway donor plasmid

    Mutation

    no stop codon

    Promoter

    none

    Available Since

    Oct. 18, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pSV HA eIF2beta WT

    Plasmid

    #45898

    Depositor

    Louise Larose

    Article

    Kebache et al Proc Natl Acad Sci U S A. 2002 Apr 16;99

    Insert

    Eif2s2 (Eif2s2 Mouse)
    

    Tags

    HA

    Expression

    Mammalian

    Promoter

    SV40

    Available Since

    June 16, 2014

    Availability

    Academic Institutions and Nonprofits only

40. pMT1100

    Plasmid

    #8618

    Depositor

    Robert Hartley

    Article

    Hartley et al Protein Peptide Letters 1996 3:225-231

    Insert

    ribonuclease Sa3, barstar
    

    Tags

    phoA signal sequence to RNase Sa3

    Expression

    Bacterial

    Mutation

    In addition to the substitution of the barnase ge…

    Available Since

    Jan. 17, 2007

    Availability

    Academic Institutions and Nonprofits only