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pLVX-CMV-Galpha-i2 ONE-GO

Plasmid

#189736

Purpose

GPCR/G protein BRET biosensor

Depositor

Mikel Garcia-Marcos

Article

Janicot et al Cell. 2024 Feb 16:S0092-8674(24)00065-5.

Inserts

Galpha-i2 ONE-GO
GNAI2 (Gnai2 Rat)

Use

Lentiviral

Available Since

Jan. 19, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pcDNA3.1-P2X2-YFP

Plasmid

#22400

Depositor

Henry Lester

Article

Khakh et al J Neurosci. 2005 Jul 20. 25(29):6911-20.

Insert

ATP-gated P2X channel 2 (P2rx2 Rat)

Tags

YFP

Expression

Mammalian

Available Since

Dec. 10, 2009

Availability

Academic Institutions and Nonprofits only

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pAAV-EF1a-DIO-PSD95-EGFP-WPRE

Plasmid

#133785

Purpose

Cre-dependent EGFP Fluorescent reporter for overexpressed postsynaptic marker PSD95 in mammalian cell

Depositor

Michael Stryker

Article

Sun et al Proc Natl Acad Sci U S A. 2019 Oct 22;11

Insert

PSD95-EGFP (Dlg4 Rat)

Use

AAV and Cre/Lox

Tags

EGFP

Expression

Mammalian

Available Since

April 23, 2020

Availability

Academic Institutions and Nonprofits only

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FUGW-VGLUT1-pHluorin

Plasmid

#207470

Purpose

Lentiviral expression of VGLUT1-pHluorin

Depositor

Robert Edwards

Insert

VGLUT1 (Slc17a7 Rat)

Use

Lentiviral

Tags

pHluorin

Available Since

Sept. 13, 2024

Availability

Academic Institutions and Nonprofits only

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Cx43-msfGFP

Plasmid

#69007

Purpose

Expresses rat Cx43 (Gja1 CDS) with a 7 amino acid linker on the C-terminus of the Connexin linking to monomerized superfolder Green Fluorescent Protein. Expression driven by CMV promoter.

Depositor

David Spray

Article

Stout RF et al J Biol Chem. 2015 Aug 11. pii: jbc.M115.

Insert

Gja1 (Gja1 Rat)

Tags

monomerized superfolder GFP

Expression

Mammalian

Promoter

cmv

Available Since

Oct. 20, 2015

Availability

Academic Institutions and Nonprofits only

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miniCGBE1 (pBM1219)

Plasmid

#140253

Purpose

CMV promoter expression plasmid for rAPOBEC1(R33A)-nCas9-P2A-EGFP

Depositor

Keith Joung

Article

Kurt et al Nat Biotechnol. 2020 Jul 20. pii: 10.103

Insert

BE4max(R33A)-ΔUGI

Expression

Mammalian

Mutation

R33A in rAPOBEC1, D10A in Cas9

Promoter

CMV

Available Since

Aug. 5, 2020

Availability

Academic Institutions and Nonprofits only

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Stx4-pmCitrine

Plasmid

#92422

Purpose

Expression of syntaxin-4 C-terminally conjugated to mCitrine.

Depositor

Geert van den Bogaart

Article

Verboogen et al Elife. 2017 May 19;6. pii: e23525. doi:

Insert

Stx4 (Stx4 Rat)

Tags

mCitrine

Expression

Mammalian

Promoter

CMV

Available Since

July 12, 2017

Availability

Academic Institutions and Nonprofits only

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pCMVtrunc F-tractinN9-39-msfGFPΔN7

Plasmid

#231552

Purpose

Mammalian expression of C-terminally truncated F-tractin fused to N-terminally truncated monomeric sfGFP, for actin filament organization measurements using fluorescence polarization microscopy

Depositor

Manos Mavrakis

Article

Martins et al Cell. 2025 Apr 1:S0092-8674(25)00276-4.

Insert

F-tractin (Itpka Rat)

Tags

msfGFP

Expression

Mammalian

Promoter

CMVtrunc

Available Since

Jan. 30, 2025

Availability

Academic Institutions and Nonprofits only

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pCMV rat SAPAP1

Plasmid

#47940

Purpose

Expresses rat SAPAP1 in mammalian cells

Depositor

Yutaka Hata, Yoshimi Takai

Article

Takeuchi et al J Biol Chem. 1997 May 2;272(18):11943-51

Insert

SAPAP1 (Dlgap1 Rat)

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 9, 2013

Availability

Academic Institutions and Nonprofits only

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pAAV-EF1a-FLEX(frt)-GCaMP6f-WPRE

Plasmid

#118273

Purpose

pAAV vector for flippase dependent GCaMP6f expression under the control of EF1a promoter

Depositor

Naoshige Uchida

Article

Menegas et al Nat Neurosci. 2018 Sep 3. pii: 10.1038/s

Insert

GCaMP6f

Use

AAV

Tags

6xHis (N terminal on insert), T7 epitope, and Xpr…

Expression

Mammalian

Promoter

EF1a

Available Since

Dec. 20, 2018

Availability

Academic Institutions and Nonprofits only

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Lamp1-mCherry-RUSH

Plasmid

#202799

Purpose

RUSH cargo: Str-KDEL_IRES_SBP-mCherry-Lamp1

Depositor

Juan Bonifacino

Article

Chen et al J Cell Biol. 2017 Dec 4;216(12):4141-415

Insert

Lamp1 (Lamp1 Rat)

Tags

mCherry

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 18, 2023

Availability

Academic Institutions and Nonprofits only

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CMV-mEGFP-CREB

Plasmid

#137001

Purpose

mEGFP-CREB donor for G-CREB

Depositor

Ryohei Yasuda

Article

Laviv et al Neuron. 2019 Dec 17. pii: S0896-6273(19)

Insert

CREB1 (Creb1 Rat)

Tags

mEGFP

Expression

Mammalian

Promoter

CMV

Available Since

May 1, 2020

Availability

Academic Institutions and Nonprofits only

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pAAV2.5-THP-dsRed2

Plasmid

#80335

Purpose

Fluorescent Reporter for Dopaminergic Neuron Differentiation

Depositor

Kwang-Soo Kim

Article

Oh et al Gene Ther. 2009 Mar;16(3):437-40. doi: 1

Insert

rat TH promoter (Th Rat)

Use

AAV

Promoter

rat Tyrosine hydroxylase

Available Since

Oct. 21, 2016

Availability

Academic Institutions and Nonprofits only

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PLEX-PGK-LAMP1-HaloTag

Plasmid

#164216

Purpose

pLEX lentivirus backbone expresses HaloTag tagged LAMP1 under PGK promoter

Depositor

Jennifer Lippincott-Schwartz

Article

Liao et al Cell. 2019 Sep 19;179(1):147-164.e20. do

Insert

LAMP1 (Lamp1 Rat)

Use

Lentiviral

Tags

HaloTag

Promoter

PGK

Available Since

Jan. 26, 2021

Availability

Academic Institutions and Nonprofits only

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pGP-CMV-jGCaMP7c variant 1513

Plasmid

#105320

Purpose

Mammalian expression of ultrasensitive protein calcium sensor

Depositor

Douglas Kim, GENIE Project

Article

Dana et al Nat Methods. 2019 Jul;16(7):649-657. doi

Insert

jGCaMP7c variant 1513

Tags

T7 epitope, Xpress tag, 6xHis

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 23, 2018

Availability

Academic Institutions and Nonprofits only

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pRK7-HA-S6K1-F5A-E389-deltaCT

Plasmid

#8990

Depositor

John Blenis

Article

Schalm et al Curr Biol. 2002 Apr 16. 12(8):632-9.

Insert

S6K1 (Rps6kb1 Rat)

Tags

HA

Expression

Mammalian

Mutation

F5A, T389E, stop codon at amino acid 402

Available Since

Sept. 2, 2005

Availability

Academic Institutions and Nonprofits only
pBridge-tyrosinase.σ2

Plasmid

#197415

Purpose

Expression of 1) GAL4 DNA-binding domain (BD)-tyrosinase cytosolic tail fusion protein, 2) HA epitope and nuclear localization signal (NLS) AP-2 σ2 fusion protein in yeast (yeast three-hybrid assays)

Depositor

Juan Bonifacino

Article

Mattera et al J Biol Chem. 2011 Jan 21;286(3):2022-30.

Inserts

tyrosinase cytosolic tail
AP-2 σ2

Tags

GAL4-DNA binding domain fragment, HA tag, and nuc…

Expression

Yeast

Mutation

encodes R42G substitution, contains silent substi…

Promoter

ADH1 and MET25

Available Since

March 28, 2023

Availability

Academic Institutions and Nonprofits only

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pDsRed1-CMV-Rat Npas4-DsRed1-pA

Plasmid

#233272

Purpose

To Express a Rat NPAS4-DsRed fusion protein from a CMV promoter

Depositor

Jonathan Ploski, Glenn Schafe

Article

Ploski et al PLoS One. 2011;6(8):e23760. doi: 10.1371

Insert

rat Npas4 DsRed fusion (Npas4 Rat)

Tags

DsRed1

Expression

Mammalian

Mutation

Y2D

Available Since

May 30, 2025

Availability

Academic Institutions and Nonprofits only

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pEGFP-N1-TrkB

Plasmid

#32500

Depositor

Rosalind Segal

Insert

TRKB (Ntrk2 Rat)

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 13, 2011

Availability

Academic Institutions and Nonprofits only

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pLenti-XI-Neo-GST-Constitutively Active AMPK

Plasmid

#139843

Purpose

Lentiviral expression of GST-tagged Constitutively Active rat AMPK

Depositor

Jacob Corn

Article

Liang et al Cell. 2020 Mar 19;180(6):1160-1177.e20.

Insert

AMPK (Prkaa2 Rat)

Use

Lentiviral

Tags

GST

Available Since

March 24, 2020

Availability

Academic Institutions and Nonprofits only

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