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We narrowed to 9,586 results for: Coli

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Showing: 901 - 950 of 9586 results

  16. SMT201

    Plasmid
    #134816
    Purpose
    SMT101 with TET promoter for TYMS, in vivo assays
    Depositor
    Tanja Kortemme
    Article
    Thompson et al eLife. 2020 Jul 23;9. pii: 53476. doi: 1
    Inserts
    Dihydrofolate reductase
    Thymidylate synthase
    Expression
    Bacterial
    Mutation
    Silent mutations to eliminate internal restrictio…
    Promoter
    T7 and TET
    Available Since
    July 22, 2020
    Availability
    Academic Institutions and Nonprofits only
    134816-plasmid-map-sequence-id-274005

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  17. pGBW-m4136457

    Plasmid
    #149537
    Purpose
    Bacterial expression plasmid for SARS-CoV-2 M (membrane)
    Depositor
    Ginkgo Bioworks, Benjie Chen
    Insert
    SARS-CoV-2 M (membrane) (M Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
    Tags
    6xHIS;Linker_GGSG;GST;Linker_GGSG;Cleavable TEV
    Expression
    Bacterial
    Mutation
    wt
    Promoter
    Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…
    Available Since
    May 14, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    149537-plasmid-map-sequence-id-314686

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  22. pHelper_TS_V2_ampR

    Plasmid
    #214467
    Purpose
    Helper plasmid for ORBIT in E. coli. Expresses RecT for oligo recombineering and Bxb-1 for pInt incorporation. Includes temperature sensitive origin for plasmid curing.
    Depositor
    Scott Saunders
    Article
    Saunders et al bioRxiv 2023
    Insert
    CspRecT / Bxb-1
    Use
    Synthetic Biology
    Promoter
    XylS / AraC
    Available Since
    April 9, 2024
    Availability
    Academic Institutions and Nonprofits only
    214467-plasmid-map-sequence-id-429338

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  25. pJK693

    Plasmid
    #72382
    Purpose
    Produces Escherichia coli cysteine desulfurase IscS with Ala260>Thr mutation, N-terminal His6-tag (H6EcIscS-A260T)
    Depositor
    T. J. Kappock
    Insert
    cysteine desulfurase (tRNA sulfurtransferase), PLP-dependent (iscS Escherichia coli str. K-12 substr. MG1655)
    Tags
    His6
    Expression
    Bacterial
    Mutation
    Mutated alanine-260 to threonine
    Promoter
    T7
    Available Since
    March 4, 2016
    Availability
    Academic Institutions and Nonprofits only
    72382-plasmid-map-sequence-id-134008

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  27. pCASCADE-gltA1-gltA2-udhA-GapAP1

    Plasmid
    #87151
    Purpose
    gltA1-gltA2-udhA-GapAP1 targeting guide RNA E. coli , Low Phosphate Induction
    Depositor
    Michael Lynch
    Insert
    gltA1-gltA2-udhA-GapAP1 guide array
    Expression
    Bacterial
    Promoter
    E . coli ugpB gene promoter, low phosphate induct…
    Available Since
    March 31, 2017
    Availability
    Academic Institutions and Nonprofits only
    87151-plasmid-map-sequence-id-174940

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  28. pGLU_116

    Plasmid
    #225796
    Purpose
    RS421 library variant 14271
    Depositor
    Nili Ostrov
    Insert
    14271_[Tn5]
    Use
    Synthetic Biology
    Mutation
    N/A
    Available Since
    Sept. 27, 2024
    Availability
    Academic Institutions and Nonprofits only
    225796-plasmid-map-sequence-id-447051

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  29. pGLU_80

    Plasmid
    #225781
    Purpose
    RS421 library variant 14271
    Depositor
    Nili Ostrov
    Insert
    14271_[abr]
    Use
    Synthetic Biology
    Mutation
    N/A
    Available Since
    Sept. 27, 2024
    Availability
    Academic Institutions and Nonprofits only
    225781-plasmid-map-sequence-id-447019

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  30. pGLU_98

    Plasmid
    #225811
    Purpose
    RS421 library variant 14271
    Depositor
    Nili Ostrov
    Insert
    14271_[Himar]
    Use
    Synthetic Biology
    Mutation
    N/A
    Available Since
    Sept. 27, 2024
    Availability
    Academic Institutions and Nonprofits only
    225811-plasmid-map-sequence-id-446973

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  31. pKP864

    Plasmid
    #167658
    Purpose
    Express colicinB-His6
    Depositor
    Kathleen Postle
    Insert
    cba-his6
    Expression
    Bacterial
    Mutation
    cba-his6
    Available Since
    July 9, 2021
    Availability
    Academic Institutions and Nonprofits only
    167658-plasmid-map-sequence-id-337304

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  34. pcpBpaRS_v2

    Plasmid
    #196485
    Purpose
    To express benzoylphenylalanine-specific variant of E. coli TyrRS (pBpaRS) and its cognate amber suppressor tRNA to allow UAG codon to be translated into benzoylphenylalanine.
    Depositor
    Kensaku Sakamoto
    Article
    Hino et al Methods Mol Biol. 2012;794:215-28. doi:
    Insert
    benzoylphenylalanine-specific variant of E. coli TyrRS (pBpaRS), amber suppressor tRNA
    Expression
    Mammalian
    Promoter
    SV40(synthetase), Pol III tRNA promoter (tRNA), C…
    Available Since
    March 28, 2023
    Availability
    Academic Institutions and Nonprofits only
    196485-plasmid-map-sequence-id-388866

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  35. pRelA'

    Plasmid
    #175595
    Purpose
    Tet inducible, KAN Resistant, non labelled synthesis domain of the E. coli relA gene on a low copy backbone. Used to induce controllable synthesis of ppGpp in E. coli.
    Depositor
    Greg Bokinsky
    Article
    Buke et al Curr Biol. 2022 Feb 28;32(4):870-877.e5.
    Insert
    Catalytic domain of E.coli relA gene.
    Expression
    Bacterial
    Mutation
    Only the catalytic domain of the enzyme is used (…
    Available Since
    Nov. 19, 2021
    Availability
    Academic Institutions and Nonprofits only
    175595-plasmid-map-sequence-id-348284

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  36. pcpAzpaRS_v2

    Plasmid
    #196483
    Purpose
    To express azidophenylalanine-specific variant of E. coli TyrRS (pAzpaRS) and its cognate amber suppressor tRNA to allow UAG codon to be translated into azidophenylalanine.
    Depositor
    Kensaku Sakamoto
    Article
    Hino et al Methods Mol Biol. 2012;794:215-28. doi:
    Insert
    azidophenylalanine-specific variant of E. coli TyrRS (pAzpaRS), amber suppressor tRNA
    Expression
    Mammalian
    Promoter
    SV40(synthetase), Pol III tRNA promoter (tRNA), C…
    Available Since
    Jan. 12, 2024
    Availability
    Academic Institutions and Nonprofits only
    196483-plasmid-map-sequence-id-388871

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  37. AlkB-D135T

    Plasmid
    #160433
    Purpose
    A plasmid for expression in E. coli to produce His-tag fusion protein AlkB with Asp at position 135 mutated to Thr
    Depositor
    Tao Pan
    Article
    Wang et al Nucleic Acids Res. 2021 Mar 18;49(5):e30
    Insert
    E. coli AlkB
    Tags
    His-tag
    Expression
    Bacterial
    Mutation
    Changed Aspartic acid 135 to Threonine; deleted t…
    Promoter
    T7
    Available Since
    Dec. 8, 2020
    Availability
    Academic Institutions and Nonprofits only
    160433-plasmid-map-sequence-id-318950

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  38. pGBW-m4136458

    Plasmid
    #149531
    Purpose
    Bacterial expression plasmid for SARS-CoV-2 surface glycoprotein (Spike protein)
    Depositor
    Ginkgo Bioworks, Benjie Chen
    Insert
    SARS-CoV-2 S (surface glycoprotein) (S Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
    Tags
    6xHIS;Linker_GGSG;SUMO tag;Linker_GGSG;Cleavable …
    Expression
    Bacterial
    Mutation
    wt
    Promoter
    Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…
    Available Since
    May 13, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    149531-plasmid-map-sequence-id-297078

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  42. pdUA-lacIN

    Plasmid
    #141079
    Purpose
    Plasmid vector for testing DNA binding proteins by the I-Block assay.
    Depositor
    Antal Kiss
    Article
    Szentes et al Nucleic Acids Res. 2020 Mar 18;48(5):e28
    Type
    Empty backbone
    Use
    Vector for the i-block assay designed to study dn…
    Expression
    Bacterial
    Promoter
    Escherichia coli lacIN promoter
    Available Since
    Sept. 14, 2020
    Availability
    Academic Institutions and Nonprofits only
    141079-plasmid-map-sequence-id-310860

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  47. pYihI-N-term K-R

    Plasmid
    #233091
    Purpose
    Expression of GST-YihI with N-term lysines (K) mutated to arginine (R)
    Depositor
    Michael Downey
    Article
    Baijal et al bioRxiv 2025.02.12.637445
    Insert
    YihI with N-term lysines (K) mutated to arginine (R)
    Tags
    GST
    Expression
    Bacterial
    Mutation
    N-terminal lysine residues mutated to arginine
    Available Since
    May 12, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    233091-plasmid-map-sequence-id-472237

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  48. pYihI-All K-R

    Plasmid
    #233094
    Purpose
    Expression of GST-YihI with all lysines (K) mutated to arginine (R)
    Depositor
    Michael Downey
    Article
    Baijal et al bioRxiv 2025.02.12.637445
    Insert
    GST-YihI with all lysines (K) mutated to arginine (R)
    Tags
    GST
    Expression
    Bacterial
    Mutation
    GST-YihI with all lysines (K) mutated to arginine…
    Available Since
    May 9, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    233094-plasmid-map-sequence-id-472238

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Showing: 901 - 950 of 9586 results