We narrowed to 1,640 results for: plasmid for e coli

1. pBEL1889

   Plasmid

   #139893

   Purpose

   Complementation plasmid for LetAB with LetB PLL4 deletion

   Depositor

   Gira Bhabha, Damian Ekiert

   Article

   Isom et al Cell. 2020 Apr 30;181(3):653-664.e19. do

   Insert

   LetAB
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   LetA-LetB(delta459-360)

   Promoter

   Available since

   Sept. 28, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pBEL1996

   Plasmid

   #139900

   Purpose

   Expression plasmid for LetB PLL4 deletion (without TM region)

   Depositor

   Gira Bhabha, Damian Ekiert

   Article

   Isom et al Cell. 2020 Apr 30;181(3):653-664.e19. do

   Insert

   LetB
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   LetB(43-877; delta459-360)-6xHis

   Promoter

   Available since

   Sept. 28, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pBEL2041

   Plasmid

   #139926

   Purpose

   Complementation plasmid for LetAB with 4 ring (rings 1237) LetB mutant

   Depositor

   Gira Bhabha, Damian Ekiert

   Article

   Isom et al Cell. 2020 Apr 30;181(3):653-664.e19. do

   Insert

   LetAB
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   LetA-LetB (1-391; 747-877)

   Promoter

   Available since

   Sept. 28, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pBEL1892

   Plasmid

   #139896

   Purpose

   Complementation plasmid for LetAB with LetB PLL7 deletion

   Depositor

   Gira Bhabha, Damian Ekiert

   Article

   Isom et al Cell. 2020 Apr 30;181(3):653-664.e19. do

   Insert

   LetAB
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   LetA-LetB(delta816-826, S815G, T827G)

   Promoter

   Available since

   Sept. 10, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pBEL2040

   Plasmid

   #139930

   Purpose

   Complementation plasmid for LetAB with LetB TM region replaced by PelB leader sequence

   Depositor

   Gira Bhabha, Damian Ekiert

   Article

   Isom et al Cell. 2020 Apr 30;181(3):653-664.e19. do

   Insert

   LetAB
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   LetA-(PelB-LetB(43-877))-6xHis

   Promoter

   Available since

   Sept. 10, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pBEL2071

   Plasmid

   #139928

   Purpose

   Complementation plasmid for LetAB operon with LetA and LetB genes deoverlapped

   Depositor

   Gira Bhabha, Damian Ekiert

   Article

   Isom et al Cell. 2020 Apr 30;181(3):653-664.e19. do

   Insert

   LetAB
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   LetA-LetB_deoverlapped

   Promoter

   Available since

   Sept. 9, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pBEL1859

   Plasmid

   #139906

   Purpose

   Complementation plasmid for LetAB with LetB mutant V247N

   Depositor

   Gira Bhabha, Damian Ekiert

   Article

   Isom et al Cell. 2020 Apr 30;181(3):653-664.e19. do

   Insert

   LetAB
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   LetA-LetB(V247N)

   Promoter

   Available since

   Sept. 9, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pBEL1858

   Plasmid

   #139905

   Purpose

   Complementation plasmid for LetAB with LetB mutant L246N

   Depositor

   Gira Bhabha, Damian Ekiert

   Article

   Isom et al Cell. 2020 Apr 30;181(3):653-664.e19. do

   Insert

   LetAB
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   LetA-LetB(L246N)

   Promoter

   Available since

   Sept. 9, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pBEL1890

   Plasmid

   #139894

   Purpose

   Complementation plasmid for LetAB with LetB PLL5 deletion

   Depositor

   Gira Bhabha, Damian Ekiert

   Article

   Isom et al Cell. 2020 Apr 30;181(3):653-664.e19. do

   Insert

   LetAB
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   LetA-LetB(delta581-590, K580G, Q591G)

   Promoter

   Available since

   Sept. 9, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pBEL2044

    Plasmid

    #139923

    Purpose

    Expression plasmid for 4 ring (rings 1237) LetB (without TM region) mutant

    Depositor

    Gira Bhabha, Damian Ekiert

    Article

    Isom et al Cell. 2020 Apr 30;181(3):653-664.e19. do

    Insert

    LetB
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    LetB (43-391; 747-877)

    Promoter

    Available since

    Aug. 27, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pBEL1731

    Plasmid

    #139922

    Purpose

    Expression plasmid for LetB mutant E854Bpa

    Depositor

    Gira Bhabha, Damian Ekiert

    Article

    Isom et al Cell. 2020 Apr 30;181(3):653-664.e19. do

    Insert

    LetB
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    LetB(43-877; E854Amber)-6xHis

    Promoter

    Available since

    Aug. 27, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pBEL1730

    Plasmid

    #139921

    Purpose

    Expression plasmid for LetB mutant K488Bpa

    Depositor

    Gira Bhabha, Damian Ekiert

    Article

    Isom et al Cell. 2020 Apr 30;181(3):653-664.e19. do

    Insert

    LetB
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    LetB(43-877; K488Amber)-6xHis

    Promoter

    Available since

    Aug. 27, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pBEL1729

    Plasmid

    #139920

    Purpose

    Expression plasmid for LetB mutant F833Bpa

    Depositor

    Gira Bhabha, Damian Ekiert

    Article

    Isom et al Cell. 2020 Apr 30;181(3):653-664.e19. do

    Insert

    LetB
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    LetB(43-877; F833Amber)-6xHis

    Promoter

    Available since

    Aug. 27, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pBEL1728

    Plasmid

    #139919

    Purpose

    Expression plasmid for LetB mutant F818Bpa

    Depositor

    Gira Bhabha, Damian Ekiert

    Article

    Isom et al Cell. 2020 Apr 30;181(3):653-664.e19. do

    Insert

    LetB
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    LetB(43-877; F818Amber)-6xHis

    Promoter

    Available since

    Aug. 27, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pBEL1727

    Plasmid

    #139918

    Purpose

    Expression plasmid for LetB mutant Y814Bpa

    Depositor

    Gira Bhabha, Damian Ekiert

    Article

    Isom et al Cell. 2020 Apr 30;181(3):653-664.e19. do

    Insert

    LetB
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    LetB(43-877; Y814Amber)-6xHis

    Promoter

    Available since

    Aug. 27, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pBEL1726

    Plasmid

    #139917

    Purpose

    Expression plasmid for LetB mutant W476pa

    Depositor

    Gira Bhabha, Damian Ekiert

    Article

    Isom et al Cell. 2020 Apr 30;181(3):653-664.e19. do

    Insert

    LetB
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    LetB(43-877; W476Amber)-6xHis

    Promoter

    Available since

    Aug. 27, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pBEL1725

    Plasmid

    #139916

    Purpose

    Expression plasmid for LetB mutant F468Bpa

    Depositor

    Gira Bhabha, Damian Ekiert

    Article

    Isom et al Cell. 2020 Apr 30;181(3):653-664.e19. do

    Insert

    LetB
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    LetB(43-877; F468Amber)-6xHis

    Promoter

    Available since

    Aug. 27, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pBEL1811

    Plasmid

    #139915

    Purpose

    Expression plasmid for LetB mutant L358N

    Depositor

    Gira Bhabha, Damian Ekiert

    Article

    Isom et al Cell. 2020 Apr 30;181(3):653-664.e19. do

    Insert

    LetB
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    LetB(43-877; L359N)-6xHis

    Promoter

    Available since

    Aug. 27, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pBEL1810

    Plasmid

    #139914

    Purpose

    Expression plasmid for LetB mutant L358N

    Depositor

    Gira Bhabha, Damian Ekiert

    Article

    Isom et al Cell. 2020 Apr 30;181(3):653-664.e19. do

    Insert

    LetB
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    LetB(43-877; L358N)-6xHis

    Promoter

    Available since

    Aug. 27, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pBEL1809

    Plasmid

    #139913

    Purpose

    Expression plasmid for LetB mutant L355N

    Depositor

    Gira Bhabha, Damian Ekiert

    Article

    Isom et al Cell. 2020 Apr 30;181(3):653-664.e19. do

    Insert

    LetB
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    LetB(43-877; L355N)-6xHis

    Promoter

    Available since

    Aug. 27, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pBEL1808

    Plasmid

    #139912

    Purpose

    Expression plasmid for LetB mutant V247N

    Depositor

    Gira Bhabha, Damian Ekiert

    Article

    Isom et al Cell. 2020 Apr 30;181(3):653-664.e19. do

    Insert

    LetB
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    LetB(43-877; V247N)-6xHis

    Promoter

    Available since

    Aug. 27, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pBEL1807

    Plasmid

    #139911

    Purpose

    Expression plasmid for LetB mutant L246N

    Depositor

    Gira Bhabha, Damian Ekiert

    Article

    Isom et al Cell. 2020 Apr 30;181(3):653-664.e19. do

    Insert

    LetB
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    LetB(43-877; L246N)-6xHis

    Promoter

    Available since

    Aug. 27, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pBEL1806

    Plasmid

    #139910

    Purpose

    Expression plasmid for LetB mutant L243N

    Depositor

    Gira Bhabha, Damian Ekiert

    Article

    Isom et al Cell. 2020 Apr 30;181(3):653-664.e19. do

    Insert

    LetB
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    LetB(43-877; L243N)-6xHis

    Promoter

    Available since

    Aug. 27, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pBEL1862

    Plasmid

    #139909

    Purpose

    Complementation plasmid for LetAB with LetB mutant L359N

    Depositor

    Gira Bhabha, Damian Ekiert

    Article

    Isom et al Cell. 2020 Apr 30;181(3):653-664.e19. do

    Insert

    LetAB
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    LetA-LetB(L359N)

    Promoter

    Available since

    Aug. 27, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pBEL1861

    Plasmid

    #139908

    Purpose

    Complementation plasmid for LetAB with LetB mutant L358N

    Depositor

    Gira Bhabha, Damian Ekiert

    Article

    Isom et al Cell. 2020 Apr 30;181(3):653-664.e19. do

    Insert

    LetAB
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    LetA-LetB(L358N)

    Promoter

    Available since

    Aug. 27, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pBEL1860

    Plasmid

    #139907

    Purpose

    Complementation plasmid for LetAB with LetB mutant L355N

    Depositor

    Gira Bhabha, Damian Ekiert

    Article

    Isom et al Cell. 2020 Apr 30;181(3):653-664.e19. do

    Insert

    LetAB
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    LetA-LetB(L355N)

    Promoter

    Available since

    Aug. 27, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pBEL1857

    Plasmid

    #139904

    Purpose

    Complementation plasmid for LetAB with LetB mutant L243N

    Depositor

    Gira Bhabha, Damian Ekiert

    Article

    Isom et al Cell. 2020 Apr 30;181(3):653-664.e19. do

    Insert

    LetAB
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    LetA-LetB(L243N)

    Promoter

    Available since

    Aug. 27, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pBEL1998

    Plasmid

    #139902

    Purpose

    Expression plasmid for LetB PLL6 deletion (without TM region)

    Depositor

    Gira Bhabha, Damian Ekiert

    Article

    Isom et al Cell. 2020 Apr 30;181(3):653-664.e19. do

    Insert

    LetB
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    LetB(43-877; delta703-708, Q702G, E709G)-6xHis

    Promoter

    Available since

    Aug. 27, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pBEL1997

    Plasmid

    #139901

    Purpose

    Expression plasmid for LetB PLL5 deletion (without TM region)

    Depositor

    Gira Bhabha, Damian Ekiert

    Article

    Isom et al Cell. 2020 Apr 30;181(3):653-664.e19. do

    Insert

    LetB
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    LetB(43-877; delta581-590, K580G, Q591G)-6xHis

    Promoter

    Available since

    Aug. 27, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pBEL1995

    Plasmid

    #139899

    Purpose

    Expression plasmid for LetB PLL3 deletion (without TM region)

    Depositor

    Gira Bhabha, Damian Ekiert

    Article

    Isom et al Cell. 2020 Apr 30;181(3):653-664.e19. do

    Insert

    LetB
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    LetB(43-877; delta347-360, P346G)-6xHis

    Promoter

    Available since

    Aug. 27, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pBEL1891

    Plasmid

    #139895

    Purpose

    Complementation plasmid for LetAB with LetB PLL6 deletion

    Depositor

    Gira Bhabha, Damian Ekiert

    Article

    Isom et al Cell. 2020 Apr 30;181(3):653-664.e19. do

    Insert

    LetAB
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    LetA-LetB(delta703-708, Q702G, E709G)

    Promoter

    Available since

    Aug. 27, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pBEL1887

    Plasmid

    #139891

    Purpose

    Complementation plasmid for LetAB with LetB PLL2 deletion

    Depositor

    Gira Bhabha, Damian Ekiert

    Article

    Isom et al Cell. 2020 Apr 30;181(3):653-664.e19. do

    Insert

    LetAB
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    LetA-LetB(delta229-239, D228G, L240G)

    Promoter

    Available since

    Aug. 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pBEL1888

    Plasmid

    #139892

    Purpose

    Complementation plasmid for LetAB with LetB PLL3 deletion

    Depositor

    Gira Bhabha, Damian Ekiert

    Article

    Isom et al Cell. 2020 Apr 30;181(3):653-664.e19. do

    Insert

    LetAB
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    LetA-LetB(delta347-360, P346G)

    Promoter

    Available since

    Aug. 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pBEL1886

    Plasmid

    #139890

    Purpose

    Complementation plasmid for LetAB with LetB PLL1 deletion

    Depositor

    Gira Bhabha, Damian Ekiert

    Article

    Isom et al Cell. 2020 Apr 30;181(3):653-664.e19. do

    Insert

    LetAB
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    LetA-LetB(delta114-128, P113G)

    Promoter

    Available since

    Aug. 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pBEL2009

    Plasmid

    #139884

    Purpose

    Complementation plasmid for LetAB with 6 ring (rings 123457) LetB mutant

    Depositor

    Gira Bhabha, Damian Ekiert

    Article

    Isom et al Cell. 2020 Apr 30;181(3):653-664.e19. do

    Insert

    LetAB
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    LetA-LetB(delta634-746)

    Promoter

    Available since

    Aug. 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pBEL2008

    Plasmid

    #139883

    Purpose

    Complementation plasmid for LetAB with 5 ring (rings 12347) LetB mutant

    Depositor

    Gira Bhabha, Damian Ekiert

    Article

    Isom et al Cell. 2020 Apr 30;181(3):653-664.e19. do

    Insert

    LetAB
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    LetA-LetB(delta515-746)

    Promoter

    Available since

    Aug. 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pBEL2007

    Plasmid

    #139882

    Purpose

    Complementation plasmid for LetAB with 4 ring (rings 1567) LetB mutant

    Depositor

    Gira Bhabha, Damian Ekiert

    Article

    Isom et al Cell. 2020 Apr 30;181(3):653-664.e19. do

    Insert

    LetAB
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    LetA-LetB(delta161-514)

    Promoter

    Available since

    Aug. 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pBEL2006

    Plasmid

    #139881

    Purpose

    Complementation plasmid for LetAB with 3 ring (rings 167) LetB mutant

    Depositor

    Gira Bhabha, Damian Ekiert

    Article

    Isom et al Cell. 2020 Apr 30;181(3):653-664.e19. do

    Insert

    LetAB
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    LetA-LetB(delta161-634)

    Promoter

    Available since

    Aug. 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pBEL1755

    Plasmid

    #139879

    Purpose

    Expression plasmid for naturally occurring 8xMCE protein (without TM region)

    Depositor

    Gira Bhabha, Damian Ekiert

    Article

    Isom et al Cell. 2020 Apr 30;181(3):653-664.e19. do

    Insert

    Denitrovibrio_acetiphilus_Dacet_0211
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Denitrovibrio_acetiphilus_Dacet_0211(41-988)-6xHis

    Promoter

    Available since

    Aug. 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pBEL1753

    Plasmid

    #139878

    Purpose

    Expression plasmid for naturally occurring 6xMCE protein (without TM region)

    Depositor

    Gira Bhabha, Damian Ekiert

    Article

    Isom et al Cell. 2020 Apr 30;181(3):653-664.e19. do

    Insert

    Pseudomonas_aeruginosa_PA4689
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Pseudomonas_aeruginosa_PA4689(41-768)-6xHis

    Promoter

    Available since

    Aug. 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

41. pBEL1752

    Plasmid

    #139877

    Purpose

    Expression plasmid for naturally occurring 5xMCE protein (without TM region)

    Depositor

    Gira Bhabha, Damian Ekiert

    Article

    Isom et al Cell. 2020 Apr 30;181(3):653-664.e19. do

    Insert

    Nitrincola_nitratireducens_D791_00999
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Nitrincola_nitratireducens_D791_00999(47-644)-6xH…

    Promoter

    Available since

    Aug. 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

42. pBEL1751

    Plasmid

    #139876

    Purpose

    Expression plasmid for naturally occurring 4xMCE protein (without TM region)

    Depositor

    Gira Bhabha, Damian Ekiert

    Article

    Isom et al Cell. 2020 Apr 30;181(3):653-664.e19. do

    Insert

    Desulfocapsa_sulfexigens_UWK_02604
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Desulfocapsa_sulfexigens_UWK_02604(38-533)-6xHis

    Promoter

    Available since

    Aug. 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

43. pBEL1584

    Plasmid

    #139875

    Purpose

    Expression plasmid of rings 2 and 3 of LetB for crystallography

    Depositor

    Gira Bhabha, Damian Ekiert

    Article

    Isom et al Cell. 2020 Apr 30;181(3):653-664.e19. do

    Insert

    LetB
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    LetB(159-383)-6xHis

    Promoter

    Available since

    Aug. 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

44. pJF6

    Plasmid

    #21678

    Depositor

    James Haber

    Article

    Fishman-Lobell et al Mol Cell Biol. 1992 Mar . 12(3):1292-303

    Insert

    LacZ (lacZ E. coli)
    

    Use

    Centromeric plasmid

    Tags

    Expression

    Mutation

    HO digestion site in one of the LacZ genes

    Promoter

    Available since

    Sept. 8, 2009

    Availability

    Academic Institutions and Nonprofits only

45. pJF5

    Plasmid

    #21679

    Depositor

    James Haber

    Article

    Fishman-Lobell et al Mol Cell Biol. 1992 Mar . 12(3):1292-303

    Insert

    LacZ (lacZ E. coli)
    

    Use

    Centromeric plasmid

    Tags

    Expression

    Mutation

    HO digestion site in one of the LacZ genes

    Promoter

    Available since

    Sept. 8, 2009

    Availability

    Academic Institutions and Nonprofits only

46. pARC8-LambdaRedBeta-EcSSB-EcMutLE32K

    Plasmid

    #162576

    Purpose

    Arabinose inducible recombineering plasmid encoding Lambda-Red Beta with EcSSB for use with dsDNA templates and enhanced recombination efficiency. Contains a dominant negative mismatch repair protein.

    Depositor

    George Church

    Article

    Filsinger et al Nat Chem Biol. 2021 Jan 18. pii: 10.1038

    Inserts

    Lambda Red-Beta
    E. coli SSB
    EcMutLE32K
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    E32K mutated to give dominant negative phenotype

    Promoter

    pBAD

    Available since

    May 22, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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47. pET28-6His-SUMO-Nsp10

    Plasmid

    #154959

    Purpose

    Expresses Nsp10 from SARS-CoV-2 with a N-terminal SUMO-protease cleavable 6His-SUMO tag in E. coli

    Depositor

    Justin Kollman

    Insert

    6His-SUMO-Nsp10 (ORF1ab SARS-CoV-2)
    

    Use

    Tags

    6His-Thrombin-SUMO

    Expression

    Bacterial

    Mutation

    Promoter

    T7 Promoter

    Available since

    Aug. 5, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

48. pET28-6His-TEV-Nsp14

    Plasmid

    #154954

    Purpose

    Expresses Nsp14 from SARS-CoV-2 with a N-terminal TEV-protease cleavable 6His tag in E. coli

    Depositor

    Justin Kollman

    Insert

    6His-TEV-Nsp14 (ORF1ab SARS-CoV-2)
    

    Use

    Tags

    6His-TEV

    Expression

    Bacterial

    Mutation

    Promoter

    T7 Promoter

    Available since

    Aug. 5, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

49. pET22-Nsp10-TEV-6His

    Plasmid

    #154957

    Purpose

    Expresses Nsp10 from SARS-CoV-2 with a C-terminal TEV-protease cleavable 6His tag in E. coli

    Depositor

    Justin Kollman

    Insert

    Nsp10-TEV-6His (ORF1ab SARS-CoV-2)
    

    Use

    Tags

    TEV-6His

    Expression

    Bacterial

    Mutation

    Promoter

    T7 Promoter

    Available since

    July 21, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

50. pET28-Nsp14-TEV-6His

    Plasmid

    #154952

    Purpose

    Expresses Nsp14 from SARS-CoV-2 with a C-terminal TEV-protease cleavable 6His tag in E. coli

    Depositor

    Justin Kollman

    Insert

    Nsp14-TEV-6His (ORF1ab SARS-CoV-2)
    

    Use

    Tags

    TEV-6His

    Expression

    Bacterial

    Mutation

    Promoter

    T7 Promoter

    Available since

    Aug. 5, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge