We narrowed to 921 results for: Gatc

1. pX330_ATP1A1_G7_Dual_sgRNA

   Plasmid

   #173206

   Purpose

   Coselection for HDR in human cells. Vector for dual expression of ATP1A1 G7 sgRNA in combination with a user-specified sgRNA from two independent U6 promoters. Cloning of oligos using BbsI sites.

   Depositor

   Yannick Doyon

   Article

   Levesque et al Nat Commun. 2022 Oct 7;13(1):5909. doi:

   Insert

   ATP1A1 G7 sgRNA + user-specified sgRNA + SpCas9
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Dual U6 promoters

   Available since

   Nov. 3, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. Intron-Tagging-pX330-Cas9-mCherry

   Plasmid

   #159742

   Purpose

   Expresses Cas9-2A-mCherry and a sgRNA excising EGFP flanked by a splice acceptor and a splice donor from the Intron-Tagging-EGFP-Donor plasmid.

   Depositor

   Stefan Kubicek

   Article

   Reicher et al Genome Res. 2020 Dec;30(12):1846-1855. d

   Insert

   donor plasmid targeting sgRNA
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Nov. 18, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. LIN52 D6.4 gRNA

   Plasmid

   #90735

   Purpose

   3rd generation lentiviral gRNA plasmid targeting human LIN52

   Depositor

   Iain Cheeseman

   Article

   McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

   Insert

   LIN52 (Guide Designation D6.4)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   U6

   Available since

   July 24, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. pSECC-sgTom

   Plasmid

   #138661

   Purpose

   Expresses a Tomato-targeting sgRNA, Cas9, and Cre-recombinase

   Depositor

   Reuben Shaw

   Article

   Hollstein et al Cancer Discov. 2019 Nov;9(11):1606-1627.

   Insert

   sgTomato
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   hU6

   Available since

   March 4, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. HOXA7 (murine) His-tag pET

   Plasmid

   #8491

   Depositor

   Corey Largman

   Article

   Shen et al Mol Cell Biol 1997 Nov;17(11):6448-58.

   Insert

   HOXA7 (Hoxa7 Mouse)
   

   Use

   Tags

   His and T7

   Expression

   Bacterial

   Mutation

   Fusion sequence is: ggatccATG; makes protein by …

   Promoter

   Available since

   May 30, 2006

   Availability

   Academic Institutions and Nonprofits only

6. pQCascade-IS1

   Plasmid

   #140624

   Purpose

   Expresses V. cholerae TniQ, Cas8, Cas7, and Cas6 and crRNA-IS1. The crRNA-IS1 targets IS1 loci in Escherchia coli.

   Depositor

   Sheng Yang

   Article

   Zhang et al ACS Synth Biol. 2020 Jun 17. doi: 10.102

   Insert

   VchTniQ, VchCas8, VchCas7, VchCas6, crRNA-IS1
   

   Use

   CRISPR; Transposon

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   July 16, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pJET1.2-U3

   Plasmid

   #173156

   Purpose

   Single guide RNA cassette under U3 promoter

   Depositor

   Piotr Ziolkowski

   Article

   Bieluszewski et al Nat Commun. 2022 Jan 12;13(1):277. doi:

   Insert

   sgRNA cassette under U3 promoter
   

   Use

   Tags

   Expression

   Plant

   Mutation

   Promoter

   promoter region of the U3C snRNA gene (Marshallsa…

   Available since

   June 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCfB3051(gRNA X-3 XI-2 XII-2)

   Plasmid

   #73293

   Purpose

   EasyClone-MarkerFree guiding RNA vector to direct Cas9 to cut at sites X-3, XI-2, and XII-2

   Depositor

   Irina Borodina

   Article

   Jessop-Fabre et al Biotechnol J. 2016 Aug;11(8):1110-7. doi

   Insert

   guiding RNA
   

   Use

   CRISPR; Grna

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Nov. 23, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. PLK1 G5.1 gRNA

   Plasmid

   #90834

   Purpose

   3rd generation lentiviral gRNA plasmid targeting human PLK1

   Depositor

   Iain Cheeseman

   Article

   McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

   Insert

   PLK1 (Guide Designation G5.1)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   U6

   Available since

   July 17, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. sgLacZs

    Plasmid

    #171185

    Purpose

    Expresses sgRNA

    Depositor

    Han Kyoung Choe

    Article

    Kim et al Sci Rep. 2021 Jan 28;11(1):2575. doi: 10

    Insert

    hU6-sgLacZ1-hU6-sgLacZ2
    

    Use

    AAV

    Tags

    mCherry

    Expression

    Mutation

    Promoter

    U6, hSyn

    Available since

    Aug. 5, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. -962 human cyclin D1 promoter TCF (0)-(4) sites mutant pGL3Basic

    Plasmid

    #32737

    Depositor

    Frank McCormick

    Article

    Tetsu et al Nature. 1999 Apr 1;398(6726):422-6.

    Insert

    CCND1 promoter (CCND1 Human)
    

    Use

    Luciferase

    Tags

    Expression

    Mutation

    -539 TCF(0) ATGAAAG deletion; -75 TCF(1) and -68 …

    Promoter

    -962 CCND1

    Available since

    Jan. 27, 2012

    Availability

    Academic Institutions and Nonprofits only

12. pFA0055

    Plasmid

    #131774

    Purpose

    Guide RNA (gCASS5a) and Cas9 expression plasmid for cleaving pFA6 series deletion cassettes, including KanMX, hphMX and natMX. Used to perform CRISPR-Swap of alleles.

    Depositor

    Frank Albert

    Article

    Lutz et al PLoS Genet. 2019 Nov 18;15(11):e1008375.

    Insert

    20mer CASS5a guide (gCASS5a) and 5' sgRNA
    

    Use

    Tags

    Expression

    Bacterial and Yeast

    Mutation

    Promoter

    Available since

    Oct. 7, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. eSpCas9(1.1)_No_FLAG_ATP1A1-G7

    Plasmid

    #173203

    Purpose

    Expresses the ATP1A1 G7 sgRNA in combination with FLAGless eSpCas9(1.1) to target ATP1A1 intron 17. pX330-like plasmid.

    Depositor

    Yannick Doyon

    Article

    Levesque et al Nat Commun. 2022 Oct 7;13(1):5909. doi:

    Insert

    ATP1A1 G7 sgRNA + FLAGless enhanced specificity Cas9 (1.1)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6 promoter

    Available since

    Nov. 3, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pKLV2-U6gRNA5(Nelfcd-g1)-PGKpuroBFP-W

    Plasmid

    #105025

    Purpose

    Lentiviral gRNA plasmid targeting mouse Nelfcd , co-expression of TagBFP

    Depositor

    Kosuke Yusa

    Article

    Li et al Cell Rep. 2018 Jul 10;24(2):489-502. doi

    Insert

    Nelfcd (Th1l Mouse)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Sept. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pLenti-Hygro-sgTom

    Plasmid

    #138698

    Purpose

    Expresses a tomato-targeting sgRNA and Cas9

    Depositor

    Reuben Shaw

    Article

    Hollstein et al Cancer Discov. 2019 Nov;9(11):1606-1627.

    Insert

    sgTomato
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    hU6

    Available since

    Feb. 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. -962 human cyclin D1 promoter TCF (1)-(4) sites mutant pGL3Basic

    Plasmid

    #32736

    Depositor

    Frank McCormick

    Article

    Tetsu et al Nature. 1999 Apr 1;398(6726):422-6.

    Insert

    CCND1 Promoter (CCND1 Human)
    

    Use

    Luciferase

    Tags

    Expression

    Mutation

    -75 TCF(1) and -68 TCF(2) CTTTGATCTTTGCT deletion…

    Promoter

    -962 CCND1

    Available since

    Jan. 27, 2012

    Availability

    Academic Institutions and Nonprofits only

17. LIN52 D5.4 gRNA

    Plasmid

    #90734

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human LIN52

    Depositor

    Iain Cheeseman

    Article

    McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

    Insert

    LIN52 (Guide Designation D5.4)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    U6

    Available since

    July 21, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. PX330_MED13_iso1_1

    Plasmid

    #135751

    Purpose

    Encodes gRNA for 3' target of human MED13_iso1

    Depositor

    Richard M. Myers

    Article

    Savic et al Genome Res. 2015 Sep 9.

    Insert

    MED13_iso1 gRNA (MED13 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    U6

    Available since

    Jan. 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pcDNA3.1(+)-MC38-TCRA-30-picky_sticky

    Plasmid

    #138983

    Purpose

    IVT template for the alpha subunit of the mouse TCR #30 that is reactive against MC38

    Depositor

    Jeff Hammerbacher

    Article

    Gottschalk et al bioRxiv 2021.11.30.470597

    Insert

    TCRA (Tcra Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV and T7

    Available since

    Nov. 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. pcDNA3.1(+)-MC38-TCRA-32-riot_punisher

    Plasmid

    #138987

    Purpose

    IVT template for the alpha subunit of the mouse TCR #32 that is reactive against MC38

    Depositor

    Jeff Hammerbacher

    Article

    Gottschalk et al bioRxiv 2021.11.30.470597

    Insert

    TCRA (Tcra Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV and T7

    Available since

    March 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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