We narrowed to 4,062 results for: p2a

1. pGL3-SEC61B.N-BSD.P2A.miniIAA7.3xFlag

   Plasmid

   #216250

   Purpose

   HDR template to tag endogenous human SEC61B N-terminus with BSD.P2A.miniIAA7.3xFlag

   Depositor

   Elina Ikonen

   Article

   Li et al Genome Biol. 2024 Feb 26;25(1):58. doi:

   Insert

   HDR template for human SEC61B (SEC61B Human, HDR template, Synthetic)
   

   Use

   CRISPR; Hdr template

   Tags

   BSD.P2A.miniIAA7.3xFlag

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 27, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. slc1a3b:myrGCaMP6s-P2A-H2AmCherry

   Plasmid

   #170209

   Purpose

   Drives myrGCaMP6s-P2A-H2AmCherry expression with the slc1a3b promoter in astrocytes. The myrGCaMP6s calcium indicator expressed in the membrane and mCherry labels the nucleus

   Depositor

   Kelly Monk

   Article

   Chen et al Nat Neurosci. 2020 Oct;23(10):1297-1306.

   Insert

   slc1a3b (slc1a3b Zebrafish)
   

   Use

   Zebrafish

   Tags

   myrGCaMP6s-P2A-H2AmCherry

   Expression

   Mutation

   Promoter

   slc1a3b

   Available since

   June 1, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA3.1-Hyg-NLS.EGFP.P2A.Cherry.CAAX

   Plasmid

   #52421

   Purpose

   Expresses a polyprotein cleaved by peptide 2A into nuclear GFP and membrane located mCherry

   Depositor

   Bertrand Collet

   Insert

   Fusion NLS-EGFP-P2A-Cherry-CAAX
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   April 3, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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4. p2attNG-H11-short

   Plasmid

   #51546

   Purpose

   p2attNG landing pad plasmid with 400 bp homology arms to the human H11 locus (for use with TALEN-assisted recombination).

   Depositor

   Michele Calos

   Article

   Zhu et al Nucleic Acids Res. 2013 Dec 4.

   Insert

   GFP4m
   

   Use

   Human targeting

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 4, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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5. pHR_TRE_mcherry-P2A-Pcad

   Plasmid

   #133807

   Purpose

   inducible expression of mCherry and Pcad in mammalian cells

   Depositor

   Wendell Lim

   Article

   Toda et al Science. 2018 Jul 13;361(6398):156-162.

   Insert

   cadherin 3 (CDH3)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 29, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. Construct 32 - NFKBRp_mKate2_2xNLS_p2a-puroR

   Plasmid

   #82024

   Purpose

   Lentiviral construct for building stable cell lines expressing mKate2 in the presence of TNFa via puromycin selection

   Depositor

   Timothy Lu

   Article

   Perli et al Science. 2016 Aug 18. pii: aag0511.

   Insert

   mKate2-2xNLS
   

   Use

   Lentiviral and Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 14, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. Cre-p2a-tdTomato

   Plasmid

   #176837

   Purpose

   Express Cre in mammalian cells and enable FACS sorting Cre expressing cells by tdTomato expression

   Depositor

   Rudolf Jaenisch

   Article

   Ma et al Cell Stem Cell. 2022 Apr 13. pii: S1934-

   Insert

   Cre-P2A-tdTomato
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAGGS

   Available since

   Nov. 2, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAAV_CAG_FLEX_PKIα-P2A-mRuby3_WPRE

   Plasmid

   #196677

   Purpose

   AAV plasmid for mammalian Cre-dependent expression of PKIα-P2A-mRuby3 under a CAG promotor w/ WPRE enhancer. PKIα (protein kinase inhibitor α) inhibits the activity of protein kinase A (PKA).

   Depositor

   Haining Zhong

   Article

   Ma et al Nature. 2022 Nov;611(7937):762-768. doi:

   Insert

   PKIα-P2A-mRuby3
   

   Use

   AAV and Cre/Lox

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCHMP2A-NS3-blue

   Plasmid

   #223532

   Purpose

   Mammalian expression of CHMP2A fused to the hepatitis C virus protease NS3 through a short linker containing the NS3 cleavage site, along with a blue fluorescent protein.

   Depositor

   Winfried Weissenhorn

   Article

   Wang et al Viruses. 2023 Nov 22;15(12):2289. doi: 1

   Insert

   CHMP2A (CHMP2A Human)
   

   Use

   Tags

   NS3 Hepatitis C protease and mTurquoise

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Aug. 27, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCHMP2A-NS3-Green

    Plasmid

    #223440

    Purpose

    Mammalian expression of CHMP2A fused to the hepatitis C virus protease NS3 through a short linker containing the NS3 cleavage site, along with a green fluorescent protein.

    Depositor

    Winfried Weissenhorn

    Article

    Wang et al Viruses. 2023 Nov 22;15(12):2289. doi: 1

    Insert

    CHMP2A (CHMP2A Human)
    

    Use

    Tags

    NS3 Hepatitis C protease and mNeonGreen

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 27, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pMXs-RAP2A-GFP

    Plasmid

    #86124

    Purpose

    Retroviral vector expressing RAP2A-GFP-IRES-Bsd

    Depositor

    David Sabatini

    Article

    Wang et al Cell. 2017 Feb 1. pii: S0092-8674(17)300

    Insert

    RAP2A (RAP2A Human)
    

    Use

    Retroviral

    Tags

    3xFLAG and GFP

    Expression

    Mammalian

    Mutation

    Promoter

    LTR

    Available since

    Feb. 22, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCAG-SRGAP2A-EGFP

    Plasmid

    #168501

    Purpose

    Expression of full length SRGAP2 with a C-termonal EGFP fusion

    Depositor

    Franck Polleux

    Article

    Guerrier et al Cell. 2009 Sep 4;138(5):990-1004. doi: 1

    Insert

    SRGAP2A (SRGAP2 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    April 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pRK5 HA GST rap2a

    Plasmid

    #14952

    Depositor

    David Sabatini

    Article

    Sancak et al Mol Cell. 2007 Mar 23. 25(6):903-15.

    Insert

    rap2a (RAP2A Human)
    

    Use

    Tags

    HA-GST

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 10, 2007

    Availability

    Academic Institutions and Nonprofits only

14. SPLICS Po-ER Short P2A

    Plasmid

    #164114

    Purpose

    Detect the long Peroxisomes-Endoplasmic reticulum contact

    Depositor

    Marisa Brini, Tito Calì

    Article

    Vallese et al Nat Commun. 2020 Nov 27;11(1):6069. doi:

    Insert

    Split YFP Po-ER Short P2A
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pGL3-RANGAP.C-linker.miniIAA7.3xFlag.P2A.BSD

    Plasmid

    #216248

    Purpose

    HDR template to tag endogenous human RANGAP1 C-terminus with miniIAA7.3xFlag.P2A.BSD

    Depositor

    Elina Ikonen

    Article

    Li et al Genome Biol. 2024 Feb 26;25(1):58. doi:

    Insert

    HDR template for human RANGAP1 (RANGAP1 Human)
    

    Use

    CRISPR and TALEN; Hdr template

    Tags

    miniIAA7.3xFlag.P2A.BSD

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 27, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. p2attNG-H11-long

    Plasmid

    #51545

    Purpose

    p2attNG landing pad plasmid with 5 and 3kb homology arms to the human H11 locus.

    Depositor

    Michele Calos

    Article

    Zhu et al Nucleic Acids Res. 2013 Dec 4.

    Insert

    GFP4m
    

    Use

    Human targeting

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 7, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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17. AICSDP-41: ATP2A2-mEGFP

    Plasmid

    #114405

    Purpose

    Homology arms and mEGFP-linker sequence for N-terminus tagging of human ATP2A2

    Depositor

    Allen Institute for Cell Science

    Insert

    ATP2A2 Homology Arms with mEGFP-linker (ATP2A2 Human)
    

    Use

    CRISPR; Donor template

    Tags

    mEGFP-linker

    Expression

    Mammalian

    Mutation

    homology arms contain point mutations to disrupt …

    Promoter

    Available since

    Sept. 4, 2018

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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18. pBT2-4xUAS:P2A-mCerulean3

    Plasmid

    #127592

    Purpose

    This vector is used for cloning a gene driven by 4xnrUAS, to be co-expressed with mCerulean3 through P2A self-cleaving peptides.

    Depositor

    Longhou Fang

    Article

    Gu et al Science. 2019 Mar 8;363(6431):1085-1088.

    Type

    Empty backbone

    Use

    Zebrafish expression

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. p2attB-FST344-HA-CPL

    Plasmid

    #131460

    Purpose

    Encodes human Follistatin protein

    Depositor

    Michele Calos

    Article

    Guha et al Mol Ther Methods Clin Dev. 2019 Oct 14;1

    Insert

    Follistatin (FST Human)
    

    Use

    Tags

    HA

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    Available since

    May 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. pLentiCas9-P2A-BFP

    Plasmid

    #149447

    Purpose

    Expresses Cas9 and BFP in a lentiviral vector.

    Depositor

    Jonathan Chernoff

    Article

    Budagyan et al Methods Mol Biol. 2021;2262:323-334. doi

    Insert

    P2A-BFP
    

    Use

    Lentiviral

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    In frame with Cas9

    Available since

    Aug. 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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