4,143 results

1. pcDNA3-Beclin1

   Plasmid

   #21150

   Depositor

   Junying Yuan

   Article

   Shibata et al J Biol Chem. 2006 May 19. 281(20):14474-

   Insert

   PcDNA3-Beclin1 (BECN1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 13, 2009

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pDisplay-AP-CFP-TM

   Plasmid

   #20861

   Depositor

   Alice Ting

   Article

   Howarth et al Proc Natl Acad Sci U S A. 2005 May 24. 1

   Insert

   AP-CFP-TM
   

   Use

   Tags

   CFP, HA, and myc

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 21, 2009

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pRK5-myc-Cdc42-Q61L

   Plasmid

   #12974

   Depositor

   Gary Bokoch

   Insert

   Cdc42 constitutively active (CDC42 Human)
   

   Use

   Tags

   Myc

   Expression

   Mammalian

   Mutation

   Q61L Constitutively active

   Promoter

   Available since

   March 2, 2007

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pBABE-puro-mTwist

   Plasmid

   #1783

   Depositor

   Bob Weinberg

   Article

   Yang et al Cell 2004 Jun 25;117(7):927-39.

   Insert

   Twist (Twist1 Mouse)
   

   Use

   Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 10, 2005

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pCALSL-mir30

   Plasmid

   #13786

   Depositor

   Connie Cepko

   Article

   Matsuda et al Proc Natl Acad Sci U S A. 2007 Jan 16;10

   Insert

   mir30 cassette
   

   Use

   Cre/Lox

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 20, 2007

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pHsp70-Cas9

   Plasmid

   #45945

   Purpose

   The codon-optimized Cas9 nuclease under the control of the Drosophila hsp70 promoter used in Gratz, et al. (2013). Plasmid is low copy number.

   Depositor

   Melissa Harrison, Jill Wildonger, Kate O'Connor-Giles

   Article

   Gratz et al Genetics. 2013 May 24.

   Insert

   Codon optimized Cas9
   

   Use

   CRISPR

   Tags

   3x FLAG

   Expression

   Mutation

   Silent change from C to A at bp 834 of insert

   Promoter

   Available since

   July 8, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pCAG-mir30

   Plasmid

   #14758

   Depositor

   Connie Cepko

   Article

   Matsuda et al Proc Natl Acad Sci U S A. 2007 Jan 16;10

   Insert

   mir30 cassette
   

   Use

   RNAi

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 20, 2007

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pdCas9::BFP-humanized

   Plasmid

   #44247

   Purpose

   Expression of a catalytically inactive, human codon-optimized Cas9-BFP fusion under the control of Murine Stem Cell retroVirus LTR promoter for mammalian gene knockdown

   Depositor

   Stanley Qi

   Article

   Qi et al Cell. 2013 Feb 28;152(5):1173-83. doi: 1

   Insert

   dCas9 fused to BFP
   

   Use

   CRISPR and Retroviral

   Tags

   2xNLS & tagBFP

   Expression

   Mammalian

   Mutation

   D10A H840A (catalytically inactive)

   Promoter

   MSCV LTR promoter

   Available since

   April 8, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. p3113 GST-p53

   Plasmid

   #10852

   Depositor

   Peter Howley

   Article

   Huibregtse et al EMBO J. 1991 Dec . 10(13):4129-35.

   Insert

   p53 (TP53 Human)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   April 20, 2006

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pcDNA3 MEKK1 WT

    Plasmid

    #12181

    Depositor

    Gary Johnson

    Article

    Widmann et al Mol Cell Biol. 1998 Apr . 18(4):2416-29.

    Insert

    MEKK1 (Map3k1 Mouse)
    

    Use

    Tags

    His and Xpress

    Expression

    Mammalian

    Mutation

    V1467L

    Promoter

    Available since

    Sept. 22, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. Stat3 Y705F Flag pRc/CMV

    Plasmid

    #8709

    Depositor

    Jim Darnell

    Article

    Wen et al Nucleic Acids Res 1997 Jun 1;25(11):2062

    Insert

    Stat3 Y705F (Stat3 Mouse)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Y705F

    Promoter

    Available since

    April 4, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. 408 pSG5L HA E2F1

    Plasmid

    #10736

    Depositor

    William Sellers

    Article

    Sellers et al Genes Dev. 1998 Jan 1. 12(1):95-106.

    Insert

    E2F1 (E2F1 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 18, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. TS module

    Plasmid

    #26021

    Depositor

    Martin Schwartz

    Article

    Grashoff et al Nature. 2010 Jul 8. 466(7303):263-6.

    Insert

    Tension Sensor module
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 17, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pPSmOrange-N1

    Plasmid

    #31898

    Depositor

    Vladislav Verkhusha

    Article

    Subach et al Nat Methods. 2011 Jul 31;8(9):771-7. doi

    Insert

    PSmOrange
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    S21T, Q63L, F100Y, L125M, K166R, P192S (mutations…

    Promoter

    Available since

    Sept. 6, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pGFP(C3)-Vinculin

    Plasmid

    #30312

    Depositor

    Klaus Hahn

    Insert

    Vinculin (Vcl Mouse)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    F633S, V828E, L936P in mouse viniculin (NM_009502…

    Promoter

    Available since

    July 7, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pAMPK alpha2 K45R

    Plasmid

    #15992

    Depositor

    Morris Birnbaum

    Article

    Mu et al Mol Cell. 2001 May . 7(5):1085-94.

    Insert

    AMPK alpha2 (Prkaa2 Rat)
    

    Use

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    K45R

    Promoter

    CMV

    Available since

    Oct. 26, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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17. GolgiCFP

    Plasmid

    #14873

    Depositor

    Alexandra Newton

    Article

    Gallegos et al J Biol Chem. 2006 Oct 13. 281(41):30947-

    Insert

    CFP
    

    Use

    Tags

    eNOS(1-35)

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 9, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. HA-Akt DN (K179M)

    Plasmid

    #16243

    Depositor

    Mien-Chie Hung

    Article

    Zhou et al J Biol Chem. 2000 Mar 17. 275(11):8027-3

    Insert

    Akt1 (Akt1 Mouse)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Dominant negative (K179M)

    Promoter

    Available since

    Nov. 30, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pcDNA3/Myc-DNMT3L

    Plasmid

    #35523

    Depositor

    Arthur Riggs

    Article

    Chen et al J Cell Biochem. 2005 Aug 1;95(5):902-17.

    Insert

    DNMT3L (DNMT3L Human)
    

    Use

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 9, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. PCDNA3 Flag p38 alpha (agf)

    Plasmid

    #20352

    Depositor

    Roger Davis

    Article

    Enslen et al J Biol Chem. 1998 Jan 16. 273(3):1741-8.

    Insert

    pDCNA3 Flag p38 alpha (agf) (Mapk14 Mouse)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Replace Thr 180 and Tyr 182 with Ala and Phe resp…

    Promoter

    Available since

    April 14, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge