19,635 results

1. pcDNA-Flag-Kdm4b-pA

   Plasmid

   #104486

   Purpose

   Express mouse Kdm4b in mammalian cells. It has a T7 promoter for in vitro transcription. Since polyA is encoded on the vector, polyA-tailing is unnecessary.

   Depositor

   Yi Zhang

   Article

   Inoue et al Genes Dev. 2017 Oct 1;31(19):1927-1932.

   Insert

   Kdm4b (Kdm4b Mouse)
   

   Tags

   Flag epitope tag

   Expression

   Mammalian

   Available Since

   Dec. 19, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pK036.TRE-Flpe-WPRE (Supernova)

   Plasmid

   #85007

   Purpose

   For Flpe/FRT-based Supernova, pK036 should be used with pK068 etc.

   Depositor

   Takuji Iwasato

   Article

   Luo et al Sci Rep. 2016 Oct 24;6:35747. doi: 10.10

   Insert

   NLS-FLPe-WPRE
   

   Expression

   Mammalian

   Available Since

   Nov. 30, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. pJH001

   Plasmid

   #67641

   Purpose

   High copy Cas9 expression vector with LEU2 marker for yeast transformation

   Depositor

   John Wyrick

   Article

   Laughery et al Yeast. 2015 Dec;32(12):711-20. doi: 10.1

   Inserts

   Cas9
   Empty
   

   Use

   CRISPR

   Expression

   Yeast

   Available Since

   Aug. 31, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. pPB-R1R2_EF1aVP64dCas9VP64_T2A_MS2p65HSF1-IRESbsdpA

   Plasmid

   #113341

   Purpose

   Expresses VP64-dCas9-VP64 and MS2-p65-HSF1 fusion proteins

   Depositor

   Gavin Wright

   Article

   Chong et al Genome Biol. 2018 Nov 26;19(1):205. doi:

   Insert

   VP64-dCas9-VP64-T2A-MS2-p65-HSF1
   

   Use

   Piggybac

   Expression

   Mammalian

   Available Since

   Dec. 12, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCAG-rtTA

   Plasmid

   #163601

   Purpose

   Expresses rtTA in mammalian cells

   Depositor

   Takeshi Imai

   Article

   Aihara et al Cell Rep. 2021 Jun 22;35(12):109276. doi

   Insert

   rtTA
   

   Expression

   Mammalian

   Available Since

   Aug. 24, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. FUS_anion

   Plasmid

   #98656

   Purpose

   Expresses untagged FUS AA 1-163

   Depositor

   Nicolas Fawzi

   Article

   Burke et al Mol Cell. 2015 Oct 15;60(2):231-41. doi:

   Insert

   FUS (FUS Human)
   

   Expression

   Bacterial

   Available Since

   July 27, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. pDG1-PHP.eB

   Plasmid

   #220799

   Purpose

   AAV helper plasmid expressing E4, E2A, VA, AAV2 rep and PHP.eB cap genes

   Depositor

   Sam Golden

   Article

   Szelenyi et al Proc Natl Acad Sci U S A. 2024 Aug 6;121

   Insert

   E4, E2A, VA, AAV2 rep and PHP.eB cap genes
   

   Use

   AAV; Aav helper vector

   Expression

   Mammalian

   Available Since

   Aug. 1, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLenti CMV/TO GFP-MDC1 (779-2)

   Plasmid

   #26285

   Depositor

   Eric Campeau

   Insert

   Mediator of DNA damage checkpoint 1 (MDC1 Human)
   

   Use

   Lentiviral

   Tags

   GFP

   Expression

   Mammalian

   Available Since

   Oct. 25, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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9. pTP1172

   Plasmid

   #104161

   Purpose

   Bacterial expression plasmid of anti-mouse kappa chain nanobody TP1170 (3x Cysteines)

   Depositor

   Dirk Görlich

   Article

   Pleiner et al J Cell Biol. 2018 Mar 5;217(3):1143-1154

   Insert

   Anti-mouse kappa chain specific nanobody TP1170 (3xCysteines)
   

   Tags

   14xHistidine tag and NEDD8 from Brachypodium dist…

   Expression

   Bacterial

   Available Since

   Dec. 21, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. pTriEx4-H6-FGAm (FlincG3)

    Plasmid

    #49202

    Purpose

    Fluorescent reporter for cGMP

    Depositor

    John Garthwaite

    Article

    Bhargava et al Front Mol Neurosci. 2013 Sep 24;6:26. do

    Insert

    FlincG3
    

    Tags

    His tag + other tags

    Expression

    Bacterial, Insect, and Mamm…

    Mutation

    Met to Lys in cpEGFP region similar to CGaMP3

    Promoter

    CMV

    Available Since

    Feb. 24, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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11. human EB1 in pEGFP N1 to express EB1-GFP (JB131)

    Plasmid

    #39299

    Depositor

    Jennifer Tirnauer, Tim Mitchison

    Insert

    EB1 (MAPRE1 Human)
    

    Tags

    EGFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Oct. 30, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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12. pENTR5'_ubi

    Plasmid

    #27320

    Depositor

    Leonard Zon

    Article

    Mosimann et al Development. 2011 Jan . 138(1):169-77.

    Insert

    zebrafish ubiquitin promoter (ubb Zebrafish)
    

    Use

    Multisite gateway 5' entry vector

    Available Since

    Feb. 3, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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13. pLV hUbC-dCas9 KRAB-T2A-GFP

    Plasmid

    #67620

    Purpose

    Co-expressed human optimized S. pyogenese dCas9 fused to KRAB repressor domain and GFP

    Depositor

    Charles Gersbach

    Article

    Kabadi et al Nucleic Acids Res. 2014 Aug 13. pii: gku

    Insert

    humanized dead Cas9 KRAB T2A GFP
    

    Use

    CRISPR and Lentiviral

    Tags

    Flag

    Mutation

    D10A and H840A

    Available Since

    March 19, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pEBB HA RelA

    Plasmid

    #74892

    Purpose

    Express RelA in mammalian cells

    Depositor

    Colin Duckett, Ezra Burstein

    Article

    Burstein et al J Biol Chem. 2005 Jun 10;280(23):22222-3

    Insert

    RelA (RELA Human)
    

    Tags

    HA

    Expression

    Mammalian

    Promoter

    EF-1alpha promoter

    Available Since

    June 23, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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15. pgRNA-CKB

    Plasmid

    #73501

    Purpose

    gRNA expression vector (with mKate2)

    Depositor

    Bruce Conklin

    Article

    Mandegar et al Cell Stem Cell. 2016 Apr 7;18(4):541-53.

    Insert

    mKate2-T2A-Bsd
    

    Use

    CRISPR and Lentiviral

    Tags

    NLS

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    Dec. 7, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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16. pET-MBP-mSA2

    Plasmid

    #52319

    Purpose

    Solubly expresses monomeric streptavidin containing S25H mutation in E. coli as an MBP fusion. App Microbiol & Biotech 98, 6285-6295 (2014)

    Depositor

    Sheldon Park

    Article

    Demonte et al Appl Microbiol Biotechnol. 2014;98(14):6

    Insert

    monomeric streptavidin 2
    

    Tags

    FLAG, His, MBP, and TEV

    Expression

    Bacterial

    Mutation

    S25H mutation compared to streptavidin

    Promoter

    T7

    Available Since

    July 15, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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17. mCherry-MAPTau-C-10

    Plasmid

    #55077

    Purpose

    Localization: Microtubules, Excitation: 587, Emission: 610

    Depositor

    Michael Davidson

    Insert

    MAPTau (MAPT Human)
    

    Tags

    mCherry

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Aug. 29, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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18. pTlpA39-Wasabi

    Plasmid

    #86116

    Purpose

    mWasabi reporter gene gated by TlpA39

    Depositor

    Mikhail Shapiro

    Article

    Piraner et al Nat Chem Biol. 2017 Jan;13(1):75-80. doi

    Inserts

    TlpA39
    mWasabi
    

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    D341A, D135V, A217V, L236F

    Promoter

    pTlpA and pTlpA, LacI

    Available Since

    Feb. 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCMV-Frankenbody-FLAG-Halo

    Plasmid

    #175296

    Purpose

    Track mature and nascent FLAG tagged proteins in live cells

    Depositor

    Tim Stasevich

    Article

    Liu et al Genes Cells. 2021 Nov;26(11):905-926. do

    Insert

    anti-FLAG frankenbody fused with HaloTag
    

    Tags

    HaloTag

    Expression

    Mammalian

    Available Since

    Oct. 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. pORTMAGE311B

    Plasmid

    #120418

    Purpose

    Expresses Lambda Beta and a dominant negative MutL E32K allele, controlled by XylS-Pm expression system for high precision and efficient MAGE experiments at 37°C. RSF1010 Ori; Broad host-range; KanR

    Depositor

    Csaba Pál

    Article

    Szili et al bioRxiv (2018)

    Inserts

    MutL E32K
    Lambda Beta
    XylS
    

    Expression

    Bacterial

    Mutation

    E32K mutation conferring dominant mutator phenoty…

    Promoter

    Pm

    Available Since

    Feb. 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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