Addgene

We narrowed to 23,156 results for: Nov

Showing: 9451 - 9500 of 23156 results

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PMT-mKate2

Plasmid

#203785

Purpose

Brightness calibration

Depositor

Thorsten Wohland

Article

Balasubramanian et al Biophys J. 2022 Dec 6;121(23):4452-4466.

Insert

PMT-mKate2

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 11, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pLVX-Puro-mRuby-Sec23A

Plasmid

#36158

Depositor

David Stephens

Article

Hughes et al J Cell Sci. 2010 Dec 1;123(Pt 23):4032-8

Insert

Sec23A (SEC23A Human)

Use

Lentiviral

Tags

mRuby

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 27, 2015

Availability

Academic Institutions and Nonprofits only

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pEB1-coSCFP3A

Plasmid

#104015

Purpose

Plasmid encoding coSCFP3A

Depositor

Philippe Cluzel

Article

Balleza et al Nat Methods. 2018 Jan;15(1):47-51. doi:

Insert

coSCFP3A

Use

Low copy

Tags

Expression

Bacterial

Mutation

Same protein sequence as SCFP3A (codon optimized …

Promoter

proC

Available since

Dec. 14, 2017

Availability

Academic Institutions and Nonprofits only

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pLysscys255

Plasmid

#36429

Depositor

Pete Lollar, Emine Gunhan

Article

Gunhan et al Protein Expr Purif. 2008 Apr;58(2):203-9

Insert

Saporin-3/cys255

Use

Tags

Expression

Bacterial

Mutation

cys added at position 255

Promoter

T7

Available since

May 10, 2012

Availability

Academic Institutions and Nonprofits only

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pACYC-GFPM2

Plasmid

#102791

Purpose

Expresses GFP under WT T7 promoter

Depositor

Andrew Ellington

Article

Abil et al Nat Protoc. 2017 Dec;12(12):2493-2512. d

Insert

Green Fluorescent Protein

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

July 3, 2018

Availability

Academic Institutions and Nonprofits only

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pEB2-2ndVal mCherry2-L

Plasmid

#104027

Purpose

Plasmid encoding 2ndVal mCherry2-L

Depositor

Philippe Cluzel

Article

Balleza et al Nat Methods. 2018 Jan;15(1):47-51. doi:

Insert

2ndVal mCherry2-L

Use

Low copy

Tags

Expression

Bacterial

Mutation

mCherry2-L with valine inserted in 2nd position

Promoter

proC

Available since

Dec. 27, 2017

Availability

Academic Institutions and Nonprofits only

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pET-6xHis-GFP(+6)

Plasmid

#199160

Purpose

used to express a supercharged GFP variant in E. coli

Depositor

Allie Obermeyer

Article

Yeong et al ACS Cent Sci. 2020 Dec 23;6(12):2301-231

Insert

GFP(+6)

Use

Tags

MGHHHHHHGGA

Expression

Mutation

E16R, D86K, D112K, D127R, I138R, D207R, N222K, L2…

Promoter

T7

Available since

May 10, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pEB1-mGFPmut3

Plasmid

#103981

Purpose

Plasmid encoding mGFPmut3

Depositor

Philippe Cluzel

Article

Balleza et al Nat Methods. 2018 Jan;15(1):47-51. doi:

Insert

mGFPmut3

Use

Low copy

Tags

Expression

Bacterial

Mutation

Mutations relative to wild-type GFP (S65G, S72A, …

Promoter

proC

Available since

Dec. 13, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pNIC-CFP

Plasmid

#173074

Purpose

Expression vector for E. coli producing target protein with N-terminal CFP fusion

Depositor

Lari Lehtiö

Article

Sowa et al Cell Rep Methods. 2021 Dec 20;1(8):10012

Type

Empty backbone

Use

Tags

mCerulean

Expression

Bacterial

Mutation

Promoter

Available since

Nov. 15, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pEXP(V5_H2B_APEX2)

Plasmid

#107597

Purpose

Expresses V5_H2B_APEX2 in mammallian cells

Depositor

Markus Hafner

Article

Benhalevy et al Nat Methods. 2018 Dec;15(12):1074-1082.

Insert

H2B-APEX2

Use

Tags

H2B and V5

Expression

Mammalian

Mutation

Promoter

CMV Dox inducible

Available since

May 9, 2018

Availability

Academic Institutions and Nonprofits only

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pEB2-2ndVal mScarlet-I

Plasmid

#104028

Purpose

Plasmid encoding 2ndVal mScarlet-I

Depositor

Philippe Cluzel

Article

Balleza et al Nat Methods. 2018 Jan;15(1):47-51. doi:

Insert

2ndVal mScarlet-I

Use

Low copy

Tags

Expression

Bacterial

Mutation

mScarlet-I with valine inserted in 2nd position

Promoter

proC

Available since

Dec. 27, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pEB2-2ndVal Katushka

Plasmid

#104029

Purpose

Plasmid encoding 2ndVal Katushka

Depositor

Philippe Cluzel

Article

Balleza et al Nat Methods. 2018 Jan;15(1):47-51. doi:

Insert

2ndVal Katushka

Use

Low copy

Tags

Expression

Bacterial

Mutation

Katushka with valine inserted in 2nd position

Promoter

proC

Available since

Dec. 27, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pNC-BiFC-ED1

Plasmid

#193426

Purpose

Nimble cloning-compatible vector for plant BiFC

Depositor

Pu Yan

Article

Yan et al Plant Commun. 2023 Mar 13;4(2):100471. d

Type

Empty backbone

Use

Tags

Expression

Plant

Mutation

Promoter

Available since

Dec. 19, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pEB1-Sapphire

Plasmid

#103976

Purpose

Plasmid encoding Sapphire

Depositor

Philippe Cluzel

Article

Balleza et al Nat Methods. 2018 Jan;15(1):47-51. doi:

Insert

Sapphire

Use

Low copy

Tags

Expression

Bacterial

Mutation

Mutations relative to wild-type GFP (S72A, Y145F,…

Promoter

proC

Available since

Dec. 13, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
H2B-SNAPf-mTurquoise2

Plasmid

#197494

Purpose

Expressing fluorescent SNAPf-mTurquoise2 fusion protein in mammalian cells with localization in the nucleus.

Depositor

Pablo Rivera-Fuentes

Article

Martin et al Nat Chem. 2024 Jan;16(1):28-35. doi: 10.

Insert

SNAPf

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 28, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
PMT-mTurquoise2_2

Plasmid

#203776

Purpose

Brightness calibration

Depositor

Thorsten Wohland

Article

Balasubramanian et al Biophys J. 2022 Dec 6;121(23):4452-4466.

Insert

PMT-mTurquoise2 dimer

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 17, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
PMT-mTurquoise2

Plasmid

#203775

Purpose

Brightness calibration

Depositor

Thorsten Wohland

Article

Balasubramanian et al Biophys J. 2022 Dec 6;121(23):4452-4466.

Insert

PMT-mTurquoise2

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 11, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
PMT-mEGFP

Plasmid

#203777

Purpose

Brightness calibration

Depositor

Thorsten Wohland

Article

Balasubramanian et al Biophys J. 2022 Dec 6;121(23):4452-4466.

Insert

PMT-mEGFP

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 11, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pMpGWB103-Citrine

Plasmid

#79137

Purpose

Binary vectors for expression of Citrine fluorescent protein in M. polymorpha

Depositor

Yutaka Kodama

Article

Tsuboyama et al Plant Cell Physiol. 2014 Jan;55(1):229-3

Insert

Citrine

Use

Tags

Citrine

Expression

Plant

Mutation

Promoter

MpEF1a promoter

Available since

Aug. 22, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pMpGE010-gR082

Plasmid

#188553

Purpose

Binary vector for CRISPR/Cas9 targeted to MpIGPD in Marchantia polymorpha (for Agrobacterium-mediated genetic transformation)

Depositor

Yutaka Kodama

Article

Fukushima et al Plant Biotechnol (Tokyo). 2022 Dec 25;39

Insert

gR082

Use

CRISPR; Entry vector

Tags

Expression

Mutation

Promoter

Available since

May 31, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pNC-BSE401

Plasmid

#193436

Purpose

Nimble cloning-compatible vector for genome editing in plants

Depositor

Pu Yan

Article

Yan et al Plant Commun. 2023 Mar 13;4(2):100471. d

Type

Empty backbone

Use

Tags

Expression

Plant

Mutation

Promoter

Available since

Dec. 14, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pEB2-2ndVal mKate2

Plasmid

#104030

Purpose

Plasmid encoding 2ndVal mKate2

Depositor

Philippe Cluzel

Article

Balleza et al Nat Methods. 2018 Jan;15(1):47-51. doi:

Insert

2ndVal mKate2

Use

Low copy

Tags

Expression

Bacterial

Mutation

mKate2 with valine inserted in 2nd position

Promoter

proC

Available since

Dec. 27, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pNC-TRV2

Plasmid

#193402

Purpose

Nimble cloning-compatible vector for virus-induced gene silencing

Depositor

Pu Yan

Article

Yan et al Plant Commun. 2023 Mar 13;4(2):100471. d

Type

Empty backbone

Use

Tags

Expression

Plant

Mutation

Promoter

Available since

Dec. 13, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pUG-57

Plasmid

#104714

Purpose

Cloning Module

Depositor

Marco Trujillo

Article

Kowarschik et al New Phytol. 2018 Mar;217(4):1749-1763. d

Insert

Ub10 (UBQ10 Mustard Weed)

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Feb. 1, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pUG-124

Plasmid

#104635

Purpose

Level 2 construct

Depositor

Marco Trujillo

Article

Kowarschik et al New Phytol. 2018 Mar;217(4):1749-1763. d

Insert

HIS-Ubq_UBA1_HA-UBC8_GST-AvrPtoB_EL4

Use

Tags

HIS-Ubq, HA-E2, GST-E3

Expression

Bacterial

Mutation

Promoter

T7

Available since

Feb. 1, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
A0A075HNX4

Plasmid

#177695

Purpose

expresses a fatty alcohol oxidase in E. coli

Depositor

Martin Engqvist

Article

Rembeza et al Chembiochem. 2022 Jan 19;23(2):e20210051

Insert

Long chain alcohol oxidase

Use

Tags

10xHis

Expression

Bacterial

Mutation

Promoter

T7

Available since

Dec. 7, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pGM34DEST

Plasmid

#74756

Purpose

Encodes for the 5X repeat of the QUAS response element, followed by the delta pes-10 minimal promoter

Depositor

Jordan Ward, Keith Yamamoto

Article

Monsalve et al Genetics. 2019 Feb;211(2):419-430. doi:

Insert

Gateway cassette

Use

Tags

Expression

Worm

Mutation

Promoter

Available since

Oct. 23, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pBS_24x-601_MA1+MA2

Plasmid

#114361

Purpose

24x Widom 601 nucleosome sequence

Depositor

Scot Wolfe

Article

Swygert et al Proc Natl Acad Sci U S A. 2018 Dec 4;115

Insert

24X601-MA1_2

Use

Unspecified

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 7, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pEB1-mEYFP

Plasmid

#103985

Purpose

Plasmid encoding mEYFP

Depositor

Philippe Cluzel

Article

Balleza et al Nat Methods. 2018 Jan;15(1):47-51. doi:

Insert

mEYFP

Use

Low copy

Tags

Expression

Bacterial

Mutation

Mutations relative to wild-type GFP (S65G, V68L, …

Promoter

proC

Available since

Dec. 13, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pECT3

Plasmid

#75450

Purpose

Plasmid that serves as the PCR template to create genomic pdt3 insertions. Plasmid contains the R6K origin of replication so needs pir gene to replicate

Depositor

James Collins

Article

Cameron et al Nat Biotechnol. 2014 Dec;32(12):1276-81.

Insert

pdt3

Use

Tags

Expression

Bacterial

Mutation

Promoter

none

Available since

Oct. 5, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pEB1-mTurquoise2

Plasmid

#103974

Purpose

Plasmid encoding mTurquoise2

Depositor

Philippe Cluzel

Article

Balleza et al Nat Methods. 2018 Jan;15(1):47-51. doi:

Insert

mTurquoise2

Use

Low copy

Tags

Expression

Bacterial

Mutation

Mutations relative to wild-type GFP (F64L, Y66W, …

Promoter

proC

Available since

Dec. 13, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pEB1-mTurquoise

Plasmid

#103972

Purpose

Plasmid encoding mTurquoise

Depositor

Philippe Cluzel

Article

Balleza et al Nat Methods. 2018 Jan;15(1):47-51. doi:

Insert

mTurquoise

Use

Low copy

Tags

Expression

Bacterial

Mutation

Mutations relative to wild-type GFP (F64L, Y66W, …

Promoter

proC

Available since

Dec. 15, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
PMT-mScarlet

Plasmid

#203779

Purpose

Brightness calibration

Depositor

Thorsten Wohland

Article

Balasubramanian et al Biophys J. 2022 Dec 6;121(23):4452-4466.

Insert

PMT-mScarlet

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 11, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pBS_12x-601_MA2

Plasmid

#114359

Purpose

12x Widom 601 nucleosome sequence

Depositor

Scot Wolfe

Article

Swygert et al Proc Natl Acad Sci U S A. 2018 Dec 4;115

Insert

12X601-MA2

Use

Unspecified

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 19, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pNC-BiFC-ED4

Plasmid

#193429

Purpose

Nimble cloning-compatible vector for plant BiFC

Depositor

Pu Yan

Article

Yan et al Plant Commun. 2023 Mar 13;4(2):100471. d

Type

Empty backbone

Use

Tags

Expression

Plant

Mutation

Promoter

Available since

Dec. 19, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pEB1-mEmerald

Plasmid

#103982

Purpose

Plasmid encoding mEmerald

Depositor

Philippe Cluzel

Article

Balleza et al Nat Methods. 2018 Jan;15(1):47-51. doi:

Insert

mEmerald

Use

Low copy

Tags

Expression

Bacterial

Mutation

Mutations relative to wild-type GFP (F64L, S65T, …

Promoter

proC

Available since

Dec. 13, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pGM48DEST

Plasmid

#74757

Purpose

Encodes for the QS repressor gene, splice linker, and mCherry reporter gene

Depositor

Jordan Ward, Keith Yamamoto

Article

Monsalve et al Genetics. 2019 Feb;211(2):419-430. doi:

Inserts

QS
Gateway cassette

Use

Tags

Expression

Worm

Mutation

Promoter

Available since

Oct. 23, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
ACE16-NLS

Plasmid

#210727

Purpose

NIR light-activated intein-based gene expression system (C-part)

Depositor

Vladislav Verkhusha

Article

Karasev et al J Mol Biol. 2023 Dec 15;435(24):168360.

Insert

ACE16-NLS

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Dec. 21, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
TND-NES

Plasmid

#210726

Purpose

NIR light-activated intein-based gene expression system (N-part)

Depositor

Vladislav Verkhusha

Article

Karasev et al J Mol Biol. 2023 Dec 15;435(24):168360.

Insert

TND-NES

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Dec. 21, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pEB2-TurboRFP

Plasmid

#103996

Purpose

Plasmid encoding TurboRFP

Depositor

Philippe Cluzel

Article

Balleza et al Nat Methods. 2018 Jan;15(1):47-51. doi:

Insert

TurboRFP

Use

Low copy

Tags

Expression

Bacterial

Mutation

Mutations relative to eqFP578 (R32G, T68A, L79F, …

Promoter

proC

Available since

Dec. 27, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pEB1-mEGFP

Plasmid

#103979

Purpose

Plasmid encoding mEGFP

Depositor

Philippe Cluzel

Article

Balleza et al Nat Methods. 2018 Jan;15(1):47-51. doi:

Insert

mEGFP

Use

Low copy

Tags

Expression

Bacterial

Mutation

Mutations relative to wild-type GFP (F64L, S65T, …

Promoter

proC

Available since

Dec. 13, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pEB1-coVenNB

Plasmid

#104019

Purpose

Plasmid encoding coVenNB

Depositor

Philippe Cluzel

Article

Balleza et al Nat Methods. 2018 Jan;15(1):47-51. doi:

Insert

coVenNB

Use

Low copy

Tags

Expression

Bacterial

Mutation

Same protein sequence as Venus NB (codon optimize…

Promoter

proC

Available since

Dec. 14, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
TOM20-SNAPf-mTurquoise2

Plasmid

#197496

Purpose

Expressing fluorescent SNAPf-mTurquoise2 fusion protein in mammalian cells with localization in the mitochondria.

Depositor

Pablo Rivera-Fuentes

Article

Martin et al Nat Chem. 2024 Jan;16(1):28-35. doi: 10.

Insert

SNAPf

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 24, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
PNCA-mCherry

Plasmid

#67790

Purpose

Expression of MMLV proviral DNA-mCherry. Use together with pCMV-intron and VSV-G to package MMLV reporter virus

Depositor

Stephen Goff

Article

Schlesinger et al EMBO Rep. 2013 Jan;14(1):73-9. doi: 10.1

Insert

MMLV-LTR-mCherry

Use

Tags

Expression

Mutation

Promoter

Available since

Sept. 1, 2015

Availability

Academic Institutions and Nonprofits only
PMT-mEGFP_2

Plasmid

#203778

Purpose

Brightness calibration

Depositor

Thorsten Wohland

Article

Balasubramanian et al Biophys J. 2022 Dec 6;121(23):4452-4466.

Insert

PMT-mEGFP dimer

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 17, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pEB1-comTurquoise2

Plasmid

#104018

Purpose

Plasmid encoding comTurquoise2

Depositor

Philippe Cluzel

Article

Balleza et al Nat Methods. 2018 Jan;15(1):47-51. doi:

Insert

comTurquoise2

Use

Low copy

Tags

Expression

Bacterial

Mutation

Same protein sequence as mTurquoise2 (codon optim…

Promoter

proC

Available since

Dec. 14, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
c7_HLL

Plasmid

#63125

Purpose

cysteine free Mm-cpn mutant

Depositor

Tanja Kortemme

Article

Hoersch et al Nat Nanotechnol. 2013 Dec;8(12):928-32.

Insert

Mm-cpn c7_HLL (NEWENTRY )

Use

Tags

6x HIS-tag

Expression

Bacterial

Mutation

C140S-C237S-C286A-C359T-C393A-C470S-C484S

Promoter

Available since

June 26, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pLacIq-Wasabi

Plasmid

#86112

Purpose

mWasabi reporter gene driven by LacIq promoter

Depositor

Mikhail Shapiro

Article

Piraner et al Nat Chem Biol. 2017 Jan;13(1):75-80. doi

Insert

mWasabi

Use

Tags

6xHis

Expression

Bacterial

Mutation

Promoter

pLacIq

Available since

Feb. 24, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pEB1-comCerulean3

Plasmid

#104017

Purpose

Plasmid encoding comCerulean3

Depositor

Philippe Cluzel

Article

Balleza et al Nat Methods. 2018 Jan;15(1):47-51. doi:

Insert

comCerulean3

Use

Low copy

Tags

Expression

Bacterial

Mutation

Same protein sequence as mCerulean3 (codon optimi…

Promoter

proC

Available since

Dec. 14, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pECT5

Plasmid

#75451

Purpose

Plasmid that serves as the PCR template to create genomic pdt5 insertions. Plasmid contains the R6K origin of replication so needs pir gene to replicate

Depositor

James Collins

Article

Cameron et al Nat Biotechnol. 2014 Dec;32(12):1276-81.

Insert

pdt5

Use

Tags

Expression

Bacterial

Mutation

Promoter

none

Available since

Oct. 5, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge

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