167,150 results
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Plasmid#58974PurposeRNA-based iPSC generation. VEE RNA replicon 3'ORF encoding OCT4, KLF4, SOX2 separated by 2A peptides followed by an IRES then GLIS1 and a second IRES and PuroR ORFDepositorUseVenezuelan equine encephalitis (vee) virus rna re…ExpressionMammalianAvailable SinceOct. 2, 2014AvailabilityAcademic Institutions and Nonprofits only
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CMV-mito-GEM-GECO1
Plasmid#32461PurposeMammalian expression of mitochondria-targeting blue-green emission ratiometric genetically encoded Ca2+ indicator for optical imagingDepositorInsertGEM-GECO
Tagsa duplex of the mitochondrial targeting signal of…ExpressionMammalianMutationGCaMP3 L60P/K69E/N77Y/D86G/N98I/K119I/L173Q/T223S…PromoterCMVAvailable SinceDec. 5, 2011AvailabilityAcademic Institutions and Nonprofits only -
TfR-pHuji
Plasmid#61505PurposeHuman transferrin receptor tagged with pH sensitive red fluorescent protein pHujiDepositorAvailable SinceDec. 19, 2014AvailabilityAcademic Institutions and Nonprofits only -
pSL2680
Plasmid#85581PurposeReplicating base vector for constructing cpf1/CRISPR editing plasmidsDepositorTypeEmpty backboneUseCRISPR and Synthetic BiologyAvailable SinceJan. 4, 2017AvailabilityAcademic Institutions and Nonprofits only -
pHAGE-IDH1-R132C
Plasmid#116410PurposeLentiviral expression of IDH1 R132CDepositorAvailable SinceMarch 14, 2019AvailabilityAcademic Institutions and Nonprofits only -
CCR7-DuET
Plasmid#213203PurposeThe DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.DepositorAvailable SinceApril 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSEVA3b61
Plasmid#58310PurposeShuttle vector: pUC ori, pTHT15 ori for use in E. coli/B. subtilis. Cannot modify orientation of cat gene; promoter too weak in E. coli, probably transcribed via repB promoter in bicistronic manner.DepositorTypeEmpty backboneUseSynthetic BiologyExpressionBacterialAvailable SinceJuly 29, 2015AvailabilityAcademic Institutions and Nonprofits only -
pFX-EGFP-Rab7
Plasmid#174457PurposeExpress EGFP with late endosome-targeting sequence in mammalian cellsDepositorInsertEGFP with late endosome-targeting sequence
Tagssmall GTPase (Rab7)ExpressionMammalianPromoterCMVAvailable SinceFeb. 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLenti CMV Hygro DEST (w117-1)
Plasmid#17454Purpose3rd gen lentiviral Gateway destination vector, expression, CMV promoter, HygroDepositorTypeEmpty backboneUseLentiviral; Destination vectorExpressionMammalianAvailable SinceAug. 12, 2009AvailabilityAcademic Institutions and Nonprofits only -
pIVT-dCas9-mCherry
Plasmid#174443Purposein vitro transcription template for dCas9-mCherryDepositorInsertCas9 Endonuclease Dead
UseIn vitro transcription templateTagsmCherryPromoterT7 SP6Available SinceOct. 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCMV-T7-SpRY-P2A-EGFP (RTW4830)
Plasmid#139989PurposeCMV and T7 promoter expression plasmid for human codon optimized SpRY(A61R/L1111R/D1135L/S1136W/G1218K/E1219Q/N1317R/A1322R/R1333P/R1335Q/T1337R) with a c-terminal bi-partite NLS, 3x flag tag, and P2A-EGFPDepositorInserthuman codon optimized SpCas9 variant named SpRY with BPNLS-3xFLAG-P2A-EGFP
UseCRISPR; In vitro transcription; t7 promoterTagsBPNLS-3xFLAG-P2A-EGFPExpressionMammalianMutationSpRY=A61R/L1111R/D1135L/S1136W/G1218K/E1219Q/N131…PromoterCMV and T7Available SinceMarch 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
pC014 - LwCas13a-msfGFP
Plasmid#91902PurposeExpresses active LwCas13a for mammalian RNA targetingDepositorInsertLwCas13a
UseCRISPRTagsNLS and msfGFP-NLS-3xHAExpressionMammalianPromoterEF1aAvailable SinceDec. 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
pT22-GluCNZ-6His
Plasmid#213504PurposeDesigned for cytoplasmic E.coli overexpression of glucanase (β-1,3-glycosidase) for gentle yeast spheroblasting that unlike Zymolyase prevents proteolysis of intracellular components.DepositorInsertGlucanase (glucan endo-1,3-beta-glucosidase)
Tags6His tagExpressionBacterialMutation1-36 Signal Sequence DeletionPromoterT7Available SinceMarch 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-C1-MKLP1
Plasmid#70145Purposemammalian expression of MKLP1 with an eGFP fusionDepositorAvailable SinceNov. 4, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCS2-UTX-F-MT2
Plasmid#40619PurposeExpression of enzyme-dead UTX in mammalian cellsDepositorInsertUTX (KDM6A Human)
Tags6xMyc and FlagExpressionMammalianMutationchanged Histidine 1146 and Glutamic Acid 1148 to …PromoterCMVAvailable SinceNov. 5, 2012AvailabilityAcademic Institutions and Nonprofits only -
pIRESpuroN3xFLAG-UFSP2
Plasmid#204553PurposeExpresses 3xFLAG-tagged UFSP2 in mammalian cellsDepositorAvailable SinceAug. 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
hsgk1
Plasmid#83432Purposefull length human sgk1 with Flag tagDepositorAvailable SinceNov. 1, 2016AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-C1-Fibrillarin
Plasmid#26673DepositorAvailable SinceNov. 10, 2010AvailabilityAcademic Institutions and Nonprofits only -
pBID-UASC
Plasmid#35200DepositorTypeEmpty backboneUseDrosophila transgene expressionPromoterDrosophila Synthetic Core Promoter (DSCP)Available SinceJuly 26, 2012AvailabilityAcademic Institutions and Nonprofits only -
pFX-EGFP-Rab5
Plasmid#174454PurposeExpress EGFP with early endosome-targeting sequence in mammalian cellsDepositorInsertEGFP with early endosome-targeting sequence
Tagssmall GTPase (Rab5)ExpressionMammalianPromoterCMVAvailable SinceFeb. 18, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAAV_SC_4.26_STARR-seq
Plasmid#220220PurposeAAV-STARR-seq screening vector with 4.26 promoteDepositorTypeEmpty backboneUseAAVExpressionMammalianPromoter4.26Available SinceAug. 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
NYESO beta/alpha into TRAC HDRT Source (pTR 169)
Plasmid#112021PurposeDNA sequence source for amplifying an HDR template to replace endogenous human TCR with 1G4 NYESO TCR at TCR-alphaDepositorInsertNYESO beta/alpha into TRAC HDRT
UseCRISPR and Synthetic BiologyAvailable SinceSept. 1, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAAV.TBG.PI.Null.bGH
Plasmid#105536Purposeempty AAV vector with TBG promoterDepositorHas ServiceAAV8InsertNone
UseAAVExpressionMammalianPromoterTBGAvailable SinceFeb. 2, 2018AvailabilityAcademic Institutions and Nonprofits only -
dCas13d-dsRBD
Plasmid#154938PurposeBPNLS-dRfxCas13d-dsRBD-BPNLS-HADepositorInsertBPNLS-dRfxCas13d-dsRBD-BPNLS-HA
UseLentiviralTagsHAExpressionMammalianPromoterCMVAvailable SinceSept. 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-beta-3
Plasmid#27289DepositorAvailable SinceJan. 24, 2011AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3-HA-human MYCN
Plasmid#74163PurposeMammalian expression of human MycNDepositorAvailable SinceApril 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
pLM-CMV-R-Cre
Plasmid#27546PurposeLentiviral bicistronic co-expression of Cre and mCherry; linked by a P2A peptide.DepositorInsertmCherry_P2A_Cre recombinase
UseCre/Lox and LentiviralPromoterCMVAvailable SinceFeb. 25, 2011AvailabilityAcademic Institutions and Nonprofits only -
mCherry-DFCP1
Plasmid#86746PurposeN-terminal mCherry-tagged protein expression in mammalian cellsDepositorAvailable SinceJan. 25, 2017AvailabilityAcademic Institutions and Nonprofits only -
AAV-EF1A-DIO-Gephyrin.FingR-eGFP-CCR5TC
Plasmid#126217PurposeLabeling of inhibitory synapses (green fluorescence) in cre-expressing neuronsDepositorInsertGephyrin.FingR-eGFP-CCR5TC
UseAAV and Cre/LoxExpressionMammalianPromoterEF1AAvailable SinceJuly 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
pB80-hKif5b(1-560)rigor-2xmNeongreen
Plasmid#174649PurposeLive-cell marker for stable microtubulesDepositorInserthKif5B(1-560)rigor-2xmNeongreen (KIF5B Human)
TagsmNeongreen-mNeongreenExpressionMammalianMutationrigor mutation G234APromoterChicken beta-actinAvailable SinceMarch 7, 2023AvailabilityAcademic Institutions and Nonprofits only