163,682 results

1. mApple-Rab5a-7

   Plasmid

   #54944

   Purpose

   Localization: Ras GTPase, Excitation: 568, Emission: 592

   Depositor

   Michael Davidson

   Insert

   Rab5a
   

   Use

   Tags

   mApple

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Aug. 7, 2014

   Availability

   Academic Institutions and Nonprofits only

2. pQLink-GST-10xCTD

   Plasmid

   #138472

   Purpose

   Expresses GST-tagged 10xCTD heptapeptide repeat in bacteria

   Depositor

   Britt Glaunsinger

   Article

   Castaneda et al PLoS Pathog. 2020 Sep 4;16(9):e1008843.

   Insert

   Rpb1 (POLR2A Human)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   10x CTD repeat

   Promoter

   T5

   Available Since

   Oct. 13, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pDONR221-NPC1_STOP

   Plasmid

   #161461

   Purpose

   Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors. Contains a STOP codon at the end of the codon-optimized ORF sequence.

   Depositor

   RESOLUTE Consortium, Giulio Superti-Furga

   Article

   Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

   Insert

   NPC1 (NPC1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Sept. 15, 2021

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

4. PCS4 3XFLAG-PPARgamma1

   Plasmid

   #78769

   Purpose

   To overexpress PPARgamma1 in Mammalian Cells

   Depositor

   Jaewhan Song

   Article

   Kim et al Cell Death Differ. 2014 Apr;21(4):594-60

   Insert

   PPAR_1 (Pparg Mouse)
   

   Use

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV IE94

   Available Since

   June 10, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. sgGFP_3

   Plasmid

   #78165

   Purpose

   sgGFP

   Depositor

   William Hahn

   Article

   Hong et al Nat Commun. 2016 Jun 22;7:11987. doi: 10

   Insert

   GFP
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hU6

   Available Since

   June 22, 2016

   Availability

   Academic Institutions and Nonprofits only

6. tetO-TCF21

   Plasmid

   #170706

   Purpose

   doxycycline-inducible overexpression of TCF21

   Depositor

   Paul Burridge

   Article

   Romero-Tejeda et al J Mol Cell Cardiol. 2023 Jul 6:S0022-282

   Insert

   TCF21 (TCF21 Human)
   

   Use

   Lentiviral; Doxycycline inducible

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   TRE promoter, Tet-ON

   Available Since

   July 12, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. TFORF1332

   Plasmid

   #143790

   Purpose

   Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

   Depositor

   Feng Zhang

   Article

   Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

   Insert

   TFAP2E (TFAP2E Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available Since

   April 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pHR_UAS-tTS-P2A-GFP_PGK-BFP

   Plasmid

   #133808

   Purpose

   inducible expression of tTS and GFP with BFP marker in mammalian cells

   Depositor

   Wendell Lim

   Article

   Toda et al Science. 2018 Jul 13;361(6398):156-162.

   Insert

   tTS-P2A-EGFP-PGK-BFP
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   June 29, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pSpyC-GFP

   Plasmid

   #220191

   Purpose

   sgRNA expression vector - pBG42 promoter with GFP in sgRNA site

   Depositor

   Larry Halverson

   Article

   Roghair Stroud et al ACS Synth Biol. 2024 Aug 20. doi: 10.102

   Insert

   pBG42 promoter with GFP in sgRNA site
   

   Use

   CRISPR

   Tags

   Expression

   Bacterial

   Mutation

   monomeric superfolder green fluorescent protein

   Promoter

   pBG42

   Available Since

   Aug. 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pMRS-LacI-dCas9

    Plasmid

    #220194

    Purpose

    mini-Tn7 dCas9 expression vector - LacI/pLac promoter

    Depositor

    Larry Halverson

    Article

    Roghair Stroud et al ACS Synth Biol. 2024 Aug 20. doi: 10.102

    Insert

    Pseudomonas aeruginosa-codon optimized Streptococcus pyogenes dCas9
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Pseudomonas aeruginosa codon optimized

    Promoter

    LacI/pLac

    Available Since

    Aug. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. TFORF3367

    Plasmid

    #144843

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    ZNF281 (ZNF281 Human)
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available Since

    Aug. 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. c-Flag pcDNA3

    Plasmid

    #20011

    Purpose

    This pcDNA3 plasmid contains a new poly-linker and single C-terminal flag-tag sequence for expression in mammalian cells.

    Depositor

    Stephen Smale

    Article

    Sanjabi et al Genes Dev. 2005 Sep 15. 19(18):2138-51.

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Jan. 28, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. dTomato-2xrGBD

    Plasmid

    #129625

    Purpose

    Visualization of active RhoA

    Depositor

    Dorus Gadella

    Article

    Mahlandt et al J Cell Sci. 2021 Aug 6. pii: 271216. doi

    Insert

    dTomato-2xrGBD
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMVdel

    Available Since

    Feb. 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pCDH-EWSR1::WT1(-KTS)

    Plasmid

    #220565

    Purpose

    Lentiviral expression of human EWSR1::WT1(-KTS)

    Depositor

    Sean Lee

    Article

    Magrath et al Commun Biol. 2024 Apr 4;7(1):411. doi: 1

    Insert

    EWSR1::WT1(-KTS) (EWSR1, WT1 Human)
    

    Use

    Lentiviral

    Tags

    HA

    Expression

    Mutation

    Promoter

    Available Since

    July 31, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. PX333-T2A-EGFP

    Plasmid

    #197417

    Purpose

    SpCas9-T2A-EGFP with dual sgRNA cloning backbones

    Depositor

    Yeh-Hsing Lao

    Article

    Yoshinaga et al Nano Lett. 2023 Jan 17. doi: 10.1021/acs

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CBh; U6

    Available Since

    March 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. lentiCRISPR v2-sgControl

    Plasmid

    #125836

    Purpose

    KO control

    Depositor

    Boyi Gan

    Article

    Zhang et al Nat Cell Biol. 2018 Oct;20(10):1181-1192

    Insert

    Control
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    June 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pDZ148

    Plasmid

    #41950

    Depositor

    Stephen Elledge

    Article

    Zhang et al Cell. 2006 Aug 11;126(3):529-42.

    Insert

    ubiquitin specific peptidase 28 (USP28 )
    

    Use

    Retroviral

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    April 17, 2013

    Availability

    Academic Institutions and Nonprofits only

18. AAV.rTH.PI.Cre.SV40

    Plasmid

    #107788

    Purpose

    AAV vector expressing Cre from rTH promoter

    Depositor

    James M. Wilson

    Has Service

    AAV Retrograde and AAV9

    Insert

    Cre
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    rTH

    Available Since

    May 16, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pRSET mNG-GECO1

    Plasmid

    #201933

    Purpose

    Expresses mNG-GECO1 indicator in bacteria

    Depositor

    Robert Campbell

    Article

    Zarowny et al ACS Sens. 2020 Jul 24;5(7):1959-1968. do

    Insert

    mNeonGreenGECO1
    

    Use

    Tags

    HIS tag

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Aug. 14, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pLKO.1-shLacZ-1735

    Plasmid

    #115454

    Purpose

    Constitutive lentiviral expression

    Depositor

    William Hahn

    Article

    Li et al Elife. 2018 Jul 30;7. pii: 37184. doi: 1

    Insert

    shLacZ-1735
    

    Use

    Lentiviral and RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Sept. 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pENTR221-mCherry

    Plasmid

    #79509

    Purpose

    MultiSite Gateway entry clones, second fragment (attL5, attL2) mCherry RFP CDS

    Depositor

    Peter Jon Nelson

    Article

    Jackel et al Sci Rep. 2016 Oct 3;6:34365. doi: 10.103

    Insert

    mCherry RFP
    

    Use

    Multisite gateway entry clone

    Tags

    Expression

    Mutation

    Promoter

    no Promoter

    Available Since

    May 12, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pFUChW Parkin shRNA Mul1 shRNA

    Plasmid

    #78523

    Purpose

    Lentiviral vector encoding two shRNAs and mCherry reporter

    Depositor

    David Chan

    Article

    Rojansky et al Elife. 2016 Nov 17;5. pii: e17896. doi:

    Insert

    Park2 shRNA, Mul1 shRNA (Prkn, Mul1 Mouse)
    

    Use

    Lentiviral and RNAi

    Tags

    Expression

    Mutation

    Promoter

    H1

    Available Since

    Jan. 5, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pCAG-IRES2-p75NTR-GFP

    Plasmid

    #182947

    Purpose

    To express the rat p75NTR neurotrophin receptor. The gene contains an HA tag at the N-Terminal

    Depositor

    Pantelis Tsoulfas

    Insert

    p75NTR-mGFP
    

    Use

    Tags

    HA tag and mGFP

    Expression

    Mammalian

    Mutation

    The leader sequence is from preprotrypin

    Promoter

    CAG

    Available Since

    April 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pAPM-D4 miR30-TRIM28 ts3

    Plasmid

    #115864

    Purpose

    TRIM28 knockdown

    Depositor

    Jeremy Luban

    Article

    Yurkovetskiy et al Nat Microbiol. 2018 Dec;3(12):1354-1361.

    Insert

    TRIM28 shRNA (TRIM28 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    SFFV

    Available Since

    Jan. 9, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pRetrosuper USP28 shRNA-1

    Plasmid

    #15663

    Depositor

    Martin Eilers

    Article

    Popov et al Nat Cell Biol. 2007 Jul . 9(7):765-74.

    Insert

    USP28 shRNA (USP28 Human)
    

    Use

    RNAi and Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Nov. 2, 2007

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

26. pMSCV-IRES-GFP II (pMIG II)

    Plasmid

    #52107

    Purpose

    a bicistronic IRES-GFP containing retroviral vector, MCS is expanded from pMSCV-IRES-GFP

    Depositor

    Dario Vignali

    Article

    Holst et al Nat Protoc. 2006;1(1):406-17.

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Use

    Retroviral

    Tags

    IRES-EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    March 26, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pESC-LEU (V5 and HA)

    Plasmid

    #222486

    Purpose

    Co-expression of two genes in yeast with V5 and HA tags and LEU2 selection

    Depositor

    Tetsuo Matsuzaki, Kiyofumi Yamada

    Article

    Dong et al FEBS Open Bio. 2024 Oct 10. doi: 10.1002

    Type

    Empty backbone

    Use

    Tags

    HA and V5

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    Oct. 11, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pLX208-beta-arrestin2-HA-TEV219

    Plasmid

    #104846

    Purpose

    b2AR-SPARK protease component

    Depositor

    Alice Ting

    Article

    Kim et al Elife. 2017 Nov 30;6. doi: 10.7554/eLife

    Insert

    beta-arrestin2-HA-TEV219
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Jan. 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pAWP78-PVCpnf_Δpvc10

    Plasmid

    #198448

    Purpose

    Produces PVCs containing truncated spike tip protein (Pvc10).

    Depositor

    Feng Zhang

    Article

    Kreitz et al Nature. 2023 Apr;616(7956):357-364. doi:

    Insert

    PVCpnf_Δpvc10
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    March 29, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pHR_Gal4UASpyb-TATA_tBFP_pGK_mCitrine (RL407)

    Plasmid

    #194217

    Purpose

    Lentiviral transducition vector for a GAL4-responsive tagBFP reporter gene

    Depositor

    Hana El-Samad

    Article

    Weinberg et al bioRxiv 2021.04.20.440678

    Insert

    UAS-ybTATA-tagBFP; PGK-mCitrine
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    GAL4 UAS-ybTATA; PGK

    Available Since

    Sept. 10, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge