We narrowed to 13,683 results for: sequence
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Plasmid#177412PurposeTo express mCerulean3 fused to the N-terminus of the Bcl-2 family chimera protein, Bcl-XL-ActA: Bcl-XL with its membrane binding region swapped for ActA (mitochondrial targeting sequence).DepositorInsertBcl-XL-ActA, BclX-acta
TagsmCerulean3ExpressionMammalianPromoterCMVAvailable SinceJan. 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
mCerulean3-BclXL-Cb5-pEGFP-C1
Plasmid#177414PurposeTo express mCerulean3 fused to the N-terminus of the Bcl-2 family chimera protein, Bcl-XL-Cb5: Bcl-XL with its membrane binding region swapped for Cb5 (ER targeting sequence).DepositorInsertBcl-XL-Cb5, BclX-cb5
TagsmCerulean3ExpressionMammalianPromoterCMVAvailable SinceJan. 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
Venus-Bcl2-ActA-pEGFP-C1
Plasmid#177419PurposeTo express Venus fused to the N-terminus of the Bcl-2 family chimera protein, Bcl-2-ActA: Bcl-2 with its membrane binding region swapped for ActA (mitochondrial targeting sequence).DepositorInsertBcl-2-ActA, Bcl2-acta, Bcl-acta
TagsVenusExpressionMammalianPromoterCMVAvailable SinceJan. 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
mCerulean3-Bcl2-ActA-s2193
Plasmid#177420PurposeTo express mCerulean3 fused to the N-terminus of the Bcl-2 family chimera protein, Bcl-2-ActA: Bcl-2 with its membrane binding region swapped for ActA (mitochondrial targeting sequence).DepositorInsertBcl-2-ActA, Bcl2-acta, Bcl-acta
TagsmCerulean3ExpressionMammalianPromoterHuman ferritinAvailable SinceJan. 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
Venus-Bcl2-cb5-pEGFP-C1
Plasmid#177422PurposeTo express Venus fused to the N-terminus of the Bcl-2 family chimera protein, Bcl-2-Cb5: Bcl-2 with its membrane binding region swapped for Cb5 (ER targeting sequence).DepositorInsertBcl-2-Cb5, Bcl2-cb5, Bcl-cb5
TagsVenusExpressionMammalianPromoterCMVAvailable SinceJan. 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
mCerulean3-Bcl2-cb5-s2193
Plasmid#177423PurposeTo express mCerulean3 fused to the N-terminus of the Bcl-2 family chimera protein, Bcl-2-Cb5: Bcl-2 with its membrane binding region swapped for Cb5 (ER targeting sequence).DepositorInsertBcl-2-Cb5, Bcl2-cb5, Bcl-cb5
TagsmCerulean3ExpressionMammalianPromoterHuman ferritinAvailable SinceJan. 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-lambdaN-DmPABPC1-V5His6_D
Plasmid#146095PurposeInsect Expression of DmPABPC1DepositorInsertDmPABPC1 (pAbp Fly)
ExpressionInsectMutationseven silent mutations compared to the sequence g…Available SinceMay 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
lvl0 fluc CDS
Plasmid#153405PurposeFirefly luciferase coding sequenceDepositorInsertfirefly luciferase CDS
ExpressionPlantAvailable SinceOct. 22, 2020AvailabilityAcademic Institutions and Nonprofits only -
pHBS1333 (IBB-GFP-mCherry 3E splicer)-(BFP-P2A-TDP43 G368W W385G)
Plasmid#107855PurposeTo test the effect of sequence on TDP43 splicing activityDepositorInsertTARDBP mutant (TARDBP Human)
ExpressionMammalianMutationG368W and W385G in splicing reporterAvailable SinceApril 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
pHBS1187 [IBB-GFP-mCherry3E]-[BFP-P2A-TDP43 N-Q]
Plasmid#107847PurposeTo test the effect of sequence on TDP43 splicing activityDepositorInsertTARDBP mutant (TARDBP Human)
ExpressionMammalianMutationAll asparagine to glutamine in splicing reporterAvailable SinceApril 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
pHBS1201 [IBB-GFP-mCherry3E]-[BFP-P2A-TDP43 6xΦ]
Plasmid#107846PurposeTo test the effect of sequence on TDP43 splicing activityDepositorInsertTARDBP mutant (TARDBP Human)
ExpressionMammalianMutationAll hydrophobics combined in six clusters in spli…Available SinceApril 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
pHBS1185 [IBB-GFP-mCherry3E]-[BFP-P2A-TDP43 F-S]
Plasmid#107843PurposeTo test the effect of sequence on TDP43 splicing activityDepositorInsertTARDBP mutant (TARDBP Human)
ExpressionMammalianMutationAll phenylalanine to serine in splicing reporterAvailable SinceApril 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
Membrane mCherry
Plasmid#210470PurposePlasma membrane localizing mCherry using Human LCK proto-oncogene sequenceDepositorInsertLCK membrane mCherry (LCK Synthetic, A.victoria, Human)
UseSynthetic Biology; Sea urchin, mammal, zebrafishTagsmCherryAvailable SinceDec. 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pFR_HCV_xb
Plasmid#11510DepositorInsertFirefly luciferase, Hepatitis C Virus IRES sequence, Renilla, and 3'UTR short RNA binding sites
UseLuciferaseAvailable SinceMay 25, 2006AvailabilityAcademic Institutions and Nonprofits only -
pWY82
Plasmid#65721PurposePlasmid with 2.5 kb of telomere sequenceDepositorInsertTelomere
ExpressionPlantAvailable SinceOct. 30, 2015AvailabilityAcademic Institutions and Nonprofits only -
pU6-Universal
Plasmid#52694PurposeThis plasmid can be used to mutate genes in Toxoplasma gondii using the CRISPR/Cas9 system after having an appropriate protospacer cloned into it.DepositorInsertsCas9
Toxoplasma U6 upstream region - protospacer cloning sequence - chiRNA
UseCRISPR; Toxoplasma gondiiTags3X FLAG and Nuclear localization signalPromoterTgTUB1 and Toxoplasma U6Available SinceJune 6, 2014AvailabilityAcademic Institutions and Nonprofits only -
CD63-pEGFP C2
Plasmid#62964Purposefull length human CD63 cloned into pEGFP C2DepositorInsertCD63 molecule (CD63 Human)
TagsEGFPExpressionMammalianMutationCD63 has 1 silent mutation compared to the coding…PromoterCMVAvailable SinceMay 28, 2015AvailabilityAcademic Institutions and Nonprofits only -
pHREP-KD-2
Plasmid#240441PurposeSleeping Beauty vector for inducible expression of an shRNA using a shortened mir30 sequence. Allows co-selection with Puromycin.DepositorTypeEmpty backboneExpressionMammalianAvailable SinceFeb. 4, 2026AvailabilityAcademic Institutions and Nonprofits only -
pCRISPR
Plasmid#42875PurposeA crRNA expression plasmid for targeting a specific sequence.DepositorInsertCRISPR-BsaI
UseCRISPR; E.coliExpressionBacterialAvailable SinceMarch 5, 2013AvailabilityAcademic Institutions and Nonprofits only -
HO-Poly-KanMX4-HO
Plasmid#51662Purposeyeast plasmid for integration of target sequence at HO locus, Kan selectionDepositorTypeEmpty backboneUseYeast integrationExpressionYeastAvailable SinceJune 5, 2014AvailabilityAcademic Institutions and Nonprofits only -
pFR_CrPV_xb
Plasmid#11509DepositorInsertFirefly luciferase, Cricket Paralysis Virus IRES sequence, Renilla, and 3'UTR short RNA binding sites
UseLuciferaseAvailable SinceMarch 15, 2006AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-(empty)-TAG
Plasmid#138209PurposeTo facilitate restriction cloning of an RNA of interest with a TGT recognition element in the 3' regionDepositorTypeEmpty backboneUseT7 transcriptionTagsTAG sequenceExpressionMammalianPromoterCMV Promoter, T7 PromoterAvailable SinceFeb. 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
mCherry-7aa-cb5-pEGFP-C1
Plasmid#177407PurposeTo express mCherry fused to endoplasmic reticulum (ER) targeting sequence "Cb5". mCherry faces the cytosol.DepositorAvailable SinceJan. 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
pJL-TRBO
Plasmid#80082PurposeAn improved agroinfection-compatible Tobacco mosaic virus (TMV)-based transient expression vector that lacks the TMV CP gene coding sequence.DepositorTypeEmpty backboneExpressionPlantPromoterCaMV35SAvailable SinceAug. 8, 2016AvailabilityAcademic Institutions and Nonprofits only -
pSGDlucV3.0
Plasmid#119760Purposedual luciferase with FMDV StopGo sequences flanking MCSDepositorTypeEmpty backboneUseLuciferaseExpressionMammalianPromoterSV40 and T7Available SinceJan. 16, 2019AvailabilityAcademic Institutions and Nonprofits only -
pPGKPhiC31obpA
Plasmid#13795PurposeExpression of mouse codon-optimized PhiC31 recombinase with bovine growth hormone polyadenylation (bpA) sequence.DepositorInsertPhiC31o
ExpressionMammalianPromoterPGKAvailable SinceJan. 16, 2007AvailabilityAcademic Institutions and Nonprofits only -
Pcr4-12xMBS-PBS
Plasmid#52984Purposeprovide MBS-PBS alternating sequenceDepositorInsert12xMBS-PBS
ExpressionBacterialPromoternoneAvailable SinceJune 23, 2014AvailabilityAcademic Institutions and Nonprofits only -
FoRC.attBGm
Plasmid#84588PurposeDrosophila PhiC31 transgenesis vector for cloning rescue transgenes. Cloning site is flanked by FRT sequences and contains constitutive GFP reporter.DepositorTypeEmpty backboneExpressionInsectAvailable SinceDec. 14, 2016AvailabilityAcademic Institutions and Nonprofits only -
No-repeats
Plasmid#122487PurposeA control plasmid that is similar to PRExpress but lacks poly-PRE sequence. (GenBank: MF058599.1)DepositorInsertLacZ
ExpressionInsectAvailable SinceMarch 3, 2020AvailabilityAcademic Institutions and Nonprofits only -
pJL 89
Plasmid#98558PurposeUseful for making infectious clones of RNA virus cDNAs; Has 35S promoter and HDV Ribozyme sequences in T-DNA region of Agrobacterium vector.DepositorTypeEmpty backboneExpressionPlantPromoterEnh 35SAvailable SinceAug. 30, 2017AvailabilityAcademic Institutions and Nonprofits only