We narrowed to 5,953 results for: 338

1. Lenti-sgPrdm9#2/Cre

   Plasmid

   #193232

   Purpose

   Lentiviral vector expressing Cre recombinase (driven by mPGK promoter) and an sgRNA against the mouse Prdm9 gene

   Depositor

   Julien Sage

   Article

   Lee et al Cell Rep. 2023 Jan 13;42(1):111990. doi:

   Insert

   sgPrdm9#2 (Prdm9 Mouse)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Dec. 12, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pv9_MBD1_MBD-R22A-13XL-BASU

   Plasmid

   #179417

   Purpose

   Cell line generation via recombination-mediated cassette exchange (RMCE) and stable expression of MBD1.MBD-R22A-13XL-BASU

   Depositor

   Tuncay Baubec

   Article

   Villasenor et al Nat Biotechnol. 2020 Mar 2. pii: 10.1038

   Insert

   MBD1.MBD-R22A-13XL-BASU
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAGGS

   Available since

   Oct. 27, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. mRFP-PA-GFP-RhoAQ63L

   Plasmid

   #187282

   Purpose

   Express pmRFP-PAGFP-RhoA Q63L

   Depositor

   Alpha Yap

   Article

   Budnar et al Dev Cell. 2019 Jun 17;49(6):894-906.e12.

   Insert

   RhoA (RHOA Human)
   

   Use

   Tags

   mRFP-PAGFP

   Expression

   Mammalian

   Mutation

   RhoA Q63L

   Promoter

   CMV, T7

   Available since

   Oct. 18, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pTS2326

   Plasmid

   #169628

   Purpose

   Tier-2 co-expression vector encoding PhCMV-driven SEAP coupled to fluorescent reporter iRFP670 and PmPGK1-driven secreted Nluc coupled to fluorescent reporter mTagBFP2 that is insulated via a 5' insulator sequence derived from the pGL3 plasmid (PhCMV-SEAP-p2A-iRFP670-pA::pGL3-PmPGK1-IgkSS-Nluc-p2A-mTagBFP2-pA::A3-pA).

   Depositor

   Martin Fussenegger

   Article

   Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

   Insert

   PCMV-driven expression of SEAP and iRFP and pGL3-modulated PPGK-driven expression of secreted Nluc and mTagBFP2
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   PhCMV / PPGK

   Available since

   Sept. 19, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pTS2321

   Plasmid

   #169626

   Purpose

   Tier-2 co-expression vector encoding PhCMV-driven SEAP coupled to fluorescent reporter iRFP670 and PmPGK1-driven secreted Nluc coupled to fluorescent reporter mTagBFP2 that is insulated via a 5' CoTC insulator sequence (PhCMV-SEAP-p2A-iRFP670-pA::CoTC-PmPGK1-IgkSS-Nluc-p2A-mTagBFP2-pA::A3-pA).

   Depositor

   Martin Fussenegger

   Article

   Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

   Insert

   PCMV-driven expression of SEAP and iRFP and CoTC-modulated PPGK-driven expression of secreted Nluc and mTagBFP2
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   PhCMV / PPGK

   Available since

   Sept. 19, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pv6_CBX7_Chromo_TAF3_Phd-DW890/891AA

   Plasmid

   #179402

   Purpose

   Cell line generation via recombination-mediated cassette exchange (RMCE) and stable expression of CBX7_Chromo - TAF3_Phd(DW890/891AA)

   Depositor

   Tuncay Baubec

   Article

   Villasenor et al Nat Biotechnol. 2020 Mar 2. pii: 10.1038

   Insert

   CBX7_Chromo - TAF3_Phd(DW890/891AA)
   

   Use

   Tags

   AviTag and EGFP

   Expression

   Mammalian

   Mutation

   Phd domain DW890/891AA

   Promoter

   CAGGS

   Available since

   Aug. 29, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. ATP1A1_G6_T804N_Std_Dual_epegRNA_tevopreQ1

   Plasmid

   #187457

   Purpose

   Coselection for prime editing in human cells. Vector for tandem expression of ATP1A1 T804N-G6 pegRNA with a user-specified epegRNA. pU6-tevopreq1-GG-acceptor-like plasmid

   Depositor

   Yannick Doyon

   Article

   Levesque et al Nat Commun. 2022 Oct 7;13(1):5909. doi:

   Insert

   ATP1A1 G6 T804N pegRNA (ATP1A1 Human)
   

   Use

   CRISPR; Prime editing

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Tandem U6 promoters

   Available since

   July 21, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pTS2329

   Plasmid

   #169629

   Purpose

   Tier-2 co-expression vector encoding PhCMV-driven SEAP coupled to fluorescent reporter iRFP670 and PmPGK1-driven secreted Nluc coupled to fluorescent reporter mTagBFP2 preluded by a 5' spacer sequence (PhCMV-SEAP-p2A-iRFP670-pA::spacer-PmPGK1-IgkSS-Nluc-p2A-mTagBFP2-pA::A3-pA).

   Depositor

   Martin Fussenegger

   Article

   Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

   Insert

   PCMV-driven expression of SEAP and iRFP and spacer-modulated PPGK-driven expression of secreted Nluc and mTagBFP2
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   PhCMV / PPGK

   Available since

   July 13, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pTS2322

   Plasmid

   #169627

   Purpose

   Tier-2 co-expression vector encoding PhCMV-driven SEAP coupled to fluorescent reporter iRFP670 and PmPGK1-driven secreted Nluc coupled to fluorescent reporter mTagBFP2 that is insulated via a 5' Tactb insulator sequence (PhCMV-SEAP-p2A-iRFP670-pA::Tactb-PmPGK1-IgkSS-Nluc-p2A-mTagBFP2-pA::A3-pA).

   Depositor

   Martin Fussenegger

   Article

   Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

   Insert

   PCMV-driven expression of SEAP and iRFP and Tactb-modulated PPGK-driven expression of secreted Nluc and mTagBFP2
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   PhCMV / PPGK

   Available since

   June 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pTS2320

    Plasmid

    #169625

    Purpose

    Tier-2 co-expression vector encoding PhCMV-driven SEAP coupled to fluorescent reporter iRFP670 and PmPGK1-driven secreted Nluc coupled to fluorescent reporter mTagBFP2 preluded by a 5' spacer sequence (PhCMV-SEAP-p2A-iRFP670-pA::A2-pA::spacer-PmPGK1-IgkSS-Nluc-p2A-mTagBFP2-pA).

    Depositor

    Martin Fussenegger

    Article

    Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

    Insert

    PCMV-driven expression of SEAP and iRFP and spacer-modulated PPGK-driven expression of secreted Nluc and mTagBFP2
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    PhCMV / PPGK

    Available since

    June 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pTS2312

    Plasmid

    #169622

    Purpose

    Tier-2 co-expression vector encoding PhCMV-driven SEAP coupled to fluorescent reporter iRFP670 and PmPGK1-driven secreted Nluc coupled to fluorescent reporter mTagBFP2 that is insulated via a 5' CoTC insulator sequence (PhCMV-SEAP-p2A-iRFP670-pA::A2-pA::CoTC-PmPGK1-IgkSS-Nluc-p2A-mTagBFP2-pA).

    Depositor

    Martin Fussenegger

    Article

    Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

    Insert

    PCMV-driven expression of SEAP and iRFP and CoTC-modulated PPGK-driven expression of secreted Nluc and mTagBFP2
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    PhCMV / PPGK

    Available since

    June 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pTS2317

    Plasmid

    #169624

    Purpose

    Tier-2 co-expression vector encoding PhCMV-driven SEAP coupled to fluorescent reporter iRFP670 and PmPGK1-driven secreted Nluc coupled to fluorescent reporter mTagBFP2 that is insulated via a 5' insulator sequence derived from the pGL3 plasmid (PhCMV-SEAP-p2A-iRFP670-pA::A2-pA::pGL3-PmPGK1-IgkSS-Nluc-p2A-mTagBFP2-pA).

    Depositor

    Martin Fussenegger

    Article

    Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

    Insert

    PCMV-driven expression of SEAP and iRFP and pGL3-modulated PPGK-driven expression of secreted Nluc and mTagBFP2
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    PhCMV / PPGK

    Available since

    June 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pcDNA3.1(+)-GFP11-RhoA

    Plasmid

    #181860

    Purpose

    Part of FluoSTEP-RhoA biosensor; co-express with cpPKN-mRuby2-GFP1-10 (Addgene #181859).

    Depositor

    Jin Zhang

    Article

    Tenner et al Sci Adv. 2021 May 21;7(21). pii: 7/21/ea

    Insert

    GFP11-RhoA (RHOA Human)
    

    Use

    Tags

    GFP11

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. MOSMO_pcDNA6.2/EmGFP-Bsd

    Plasmid

    #176975

    Purpose

    Mammalian expression vector encoding MOSMO and EmGFP-Bsd

    Depositor

    Roger Reeves

    Article

    Moyer et al Dis Model Mech. 2023 Apr 1;16(4):dmm0497

    Insert

    MOSMO (C16orf52 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pnYC-HseIF4E_AF

    Plasmid

    #148669

    Purpose

    Bacterial Expression of HseIF4E

    Depositor

    Elisa Izaurralde

    Article

    Gruner et al Mol Cell. 2016 Nov 3;64(3):467-479. doi:

    Insert

    HseIF4E (EIF4E Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    one silent mutation compared to the sequence of i…

    Promoter

    Available since

    March 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pCAG1.1-Armc12/FLAG

    Plasmid

    #180494

    Purpose

    Expression vector of mouse armadillo repeat containing 12 (Armc12) tagged with FLAG at C-terminus. See Fig.4D of Shimada, K. et al. Proc. Natl. Acad. Sci. USA. 118 (6): e2018355118, 2021.

    Depositor

    Masahito Ikawa

    Article

    Shimada et al Proc Natl Acad Sci U S A. 2021 Feb 9;118

    Insert

    armadillo repeat containing 12 (Armc12 Mouse)
    

    Use

    Tags

    FLAG tag

    Expression

    Mammalian

    Mutation

    Promoter

    CAG promoter

    Available since

    March 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. S+Q111R

    Plasmid

    #167173

    Purpose

    Expresses the full Leptodactylus latrans Na+K+ATPase with ATP1A1-S variant with Q111R mutation in Sf9 cells

    Depositor

    Susanne Dobler

    Article

    Mohammadi et al Curr Biol. 2021 Jun 21;31(12):2530-2538.

    Inserts

    ATP1A1-S+Q111R
    ATP1B1
    

    Use

    Tags

    Expression

    Bacterial and Insect

    Mutation

    Changed Q at 111 to R

    Promoter

    P10 and PH

    Available since

    Feb. 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. S+N122D

    Plasmid

    #167174

    Purpose

    Expresses the full Leptodactylus latrans Na+K+ATPase with ATP1A1-S variant with N122D mutation in Sf9 cells

    Depositor

    Susanne Dobler

    Article

    Mohammadi et al Curr Biol. 2021 Jun 21;31(12):2530-2538.

    Inserts

    ATP1A1-S+N122D
    ATP1B1
    

    Use

    Tags

    Expression

    Bacterial and Insect

    Mutation

    Changed N at 111 to D

    Promoter

    P10 and PH

    Available since

    Feb. 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pDestTol2-actinb-kcnj1b-IRES-EGFP

    Plasmid

    #164946

    Purpose

    Tol2 construct: actinb (actb2) promoter driven zebrafish kcnj1b and EGFP

    Depositor

    GuangJun Zhang

    Article

    Silic et al Genetics. 2020 Aug;215(4):1067-1084. doi

    Insert

    kcnj1b (kcnj1b Zebrafish)
    

    Use

    Tol2 transposon destination vector

    Tags

    IRES-EGFP

    Expression

    Mutation

    Promoter

    actinb (actb2) promoter

    Available since

    Feb. 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. ST-Tstem-GFP

    Plasmid

    #162501

    Purpose

    Expresses a ST6GAL1 mutant with a GFP tag in its C-terminus in mammalian cells. The stem region of Tac is inserted within ST.

    Depositor

    Lei Lu

    Article

    Sun et al J Biol Chem. 2020 Aug 21. pii: RA120.014

    Insert

    ST6Gal1 mutant (Tac stem region is inserted within ST) (ST6GAL1, IL2RA Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    The Tac stem region is inserted within ST6Gal1

    Promoter

    Available since

    Jan. 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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