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lvl0-G [backbone]

Plasmid

#229156

Purpose

Gene backbone

Depositor

Ron Weiss

Article

Duportet et al Nucleic Acids Res. 2014 Dec 1;42(21):134

Type

Empty backbone

Use

Synthetic Biology

Available Since

Jan. 30, 2025

Availability

Academic Institutions and Nonprofits only

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lvl0-3 [backbone]

Plasmid

#229157

Purpose

3 prime backbone

Depositor

Ron Weiss

Article

Duportet et al Nucleic Acids Res. 2014 Dec 1;42(21):134

Type

Empty backbone

Use

Synthetic Biology

Available Since

Jan. 30, 2025

Availability

Academic Institutions and Nonprofits only

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lvl0-T [backbone]

Plasmid

#229158

Purpose

Terminator backbone

Depositor

Ron Weiss

Article

Duportet et al Nucleic Acids Res. 2014 Dec 1;42(21):134

Type

Empty backbone

Use

Synthetic Biology

Available Since

Jan. 30, 2025

Availability

Academic Institutions and Nonprofits only

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pGH643

Plasmid

#230834

Purpose

hu6-BsmBI-MS2_sgRNA-Ef1a-puroR-T2A-mCherry-wPRE

Depositor

Gaelen Hess

Article

Schwartz et al Nucleic Acids Res. 2025 Jun 20;53(12):gk

Type

Empty backbone

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

Jan. 17, 2025

Availability

Academic Institutions and Nonprofits only

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pGH394

Plasmid

#230851

Purpose

Ef1a-rAPOBEC1-dCas9-T2A-BlastR-wPRE

Depositor

Gaelen Hess

Article

Schwartz et al Nucleic Acids Res. 2025 Jun 20;53(12):gk

Insert

rAPOBEC1-dCas9

Use

Lentiviral

Expression

Mammalian

Available Since

Jan. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pGH398

Plasmid

#230854

Purpose

Ef1a-rAPOBEC1-n'Cas9-T2A-BlastR-wPRE

Depositor

Gaelen Hess

Article

Schwartz et al Nucleic Acids Res. 2025 Jun 20;53(12):gk

Insert

rAPOBEC1-n'Cas9

Use

Lentiviral

Expression

Mammalian

Available Since

Jan. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pGH401

Plasmid

#230852

Purpose

Ef1a-rAPOBEC1-nCas9-T2A-BlastR-wPRE

Depositor

Gaelen Hess

Article

Schwartz et al Nucleic Acids Res. 2025 Jun 20;53(12):gk

Insert

rAPOBEC1-nCas9

Use

Lentiviral

Expression

Mammalian

Available Since

Jan. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pAMC070n1a12

Plasmid

#209746

Purpose

ColA BBa_B0014 tetAp SD8 ScTrpRS rrnB1 proB SD8 Lum1PylRS BBaB10014RNAItrpA+ J23100 SD8 MmPylRS T3Te-T7Te DHFR

Depositor

Ahmed Badran

Article

Costello et al Nat Biotechnol. 2024 Sep 11. doi: 10.103

Insert

tRNA operon - ScTrpRS - Lum1PylRS - MmPylRS

Expression

Bacterial

Available Since

Oct. 18, 2024

Availability

Academic Institutions and Nonprofits only

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pEF-1α-p37-mClover2

Plasmid

#225500

Purpose

Expression of mClover2-tagged p37 protein

Depositor

David Rubinsztein

Article

Wrobel et al Sci Adv. 2024 May 3;10(18):eadl6082. doi

Insert

p37 (UBXN2B Human)

Use

Lentiviral

Tags

mClover2

Expression

Mammalian

Mutation

no

Promoter

EF1A

Available Since

Oct. 16, 2024

Availability

Academic Institutions and Nonprofits only

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pCMV5a-p37WT-FLAG

Plasmid

#225498

Purpose

Expression of FLAG-tagged wildtype p37 protein

Depositor

David Rubinsztein

Article

Wrobel et al Sci Adv. 2024 May 3;10(18):eadl6082. doi

Insert

p37 (UBXN2B Human)

Tags

FLAG

Expression

Mammalian

Mutation

no

Promoter

CMV promoter

Available Since

Oct. 16, 2024

Availability

Academic Institutions and Nonprofits only

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pCMV5a-p37SHPmut_FLAG

Plasmid

#225499

Purpose

Expression of FLAG-tagged mutated p37 protein

Depositor

David Rubinsztein

Article

Wrobel et al Sci Adv. 2024 May 3;10(18):eadl6082. doi

Insert

p37 (UBXN2B Human)

Tags

FLAG

Expression

Mammalian

Mutation

F215A/E218A/QKL220-222AAA

Promoter

CMV promoter

Available Since

Oct. 16, 2024

Availability

Academic Institutions and Nonprofits only

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Vector 1174K

Plasmid

#221017

Purpose

Fluorescent based sex separator in Anopheles species mosquitoes (SEPARATOR)

Depositor

Omar Akbari

Article

Weng et al bioRxiv [Preprint]. 2024 Jul 18:2024.07.

Insert

Engineered dobulesex splicing module (Anopheles gambiae)

Use

Synthetic Biology

Expression

Insect

Available Since

Aug. 5, 2024

Availability

Academic Institutions and Nonprofits only

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LDB34

Plasmid

#185431

Purpose

Yeast expression of Nup1 C-terminal truncation (nup1-21 allele)

Depositor

Ken Belanger, Laura Davis

Article

Bogerd et al J Cell Biol. 1994 Oct . 127(2):319-32.

Insert

NUP1

Expression

Yeast

Mutation

Truncation at amino acid 1041 from the C-term of …

Available Since

July 25, 2022

Availability

Academic Institutions and Nonprofits only

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LDB33

Plasmid

#185430

Purpose

Yeast expression of Nup1 N-terminal truncation (nup1-15 allele)

Depositor

Ken Belanger, Laura Davis

Article

Bogerd et al J Cell Biol. 1994 Oct . 127(2):319-32.

Insert

NUP1

Expression

Yeast

Mutation

Truncation of amino acids 3-191 from the N-term o…

Available Since

July 21, 2022

Availability

Academic Institutions and Nonprofits only

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pIB1884-ximD-PCPT

Plasmid

#169109

Purpose

Production of marmesin in Streptomyces xiamenensis

Depositor

Minjuan Xu

Article

Bu et al ACS Synth Biol. 2020 Sep 18;9(9):2282-22

Insert

XimD and PcPT

Use

Synthetic Biology

Available Since

May 13, 2021

Availability

Academic Institutions and Nonprofits only

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AlACR2_EYFP_pcDNA3.1

Plasmid

#161023

Purpose

Expresses AlACR2 in mammalian cells

Depositor

John Spudich

Article

Govorunova et al Proc Natl Acad Sci U S A. 2020 Sep 15;11

Insert

AlACR2

Tags

EYFP

Expression

Mammalian

Available Since

Nov. 17, 2020

Availability

Academic Institutions and Nonprofits only

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AlACR3_EYFP_pcDNA3.1

Plasmid

#161024

Purpose

Expresses AlACR3 in mammalian cells

Depositor

John Spudich

Article

Govorunova et al Proc Natl Acad Sci U S A. 2020 Sep 15;11

Insert

AlACR3

Tags

EYFP

Expression

Mammalian

Available Since

Nov. 17, 2020

Availability

Academic Institutions and Nonprofits only

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RsCCR2_EYFP_pcDNA3.1

Plasmid

#159125

Purpose

This plasmid encodes RsCCR2 rhodopsin domain

Depositor

John Spudich

Article

Sineshchekov et al mBio. 2020 Apr 21;11(2). pii: mBio.00657

Insert

RsCCR2

Tags

EYFP

Expression

Mammalian

Available Since

Sept. 10, 2020

Availability

Academic Institutions and Nonprofits only

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RsCCR1_EYFP_pcDNA3.1

Plasmid

#159124

Purpose

This plasmid encodes RsCCR1 rhodopsin domain

Depositor

John Spudich

Article

Sineshchekov et al mBio. 2020 Apr 21;11(2). pii: mBio.00657

Insert

RsCCR1

Tags

EYFP

Expression

Mammalian

Available Since

Sept. 10, 2020

Availability

Academic Institutions and Nonprofits only

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RaCCR2_EYFP_pcDNA3.1

Plasmid

#159123

Purpose

This plasmid encodes RaCCR2 rhodopsin domain

Depositor

John Spudich

Article

Sineshchekov et al mBio. 2020 Apr 21;11(2). pii: mBio.00657

Insert

RaCCR2

Tags

EYFP

Expression

Mammalian

Available Since

Sept. 10, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge