We narrowed to 21,470 results for: SPR

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pHAGE EF1α dCas9-NED

Plasmid

#109369

Purpose

Lentiviral vector for expressing effector domains fused to dCas9.

Depositor

Antal Kiss

Article

Goubert et al Methods Mol Biol. 2018;1767:395-415. doi

Type

Empty backbone

Use

CRISPR and Lentiviral

Tags

3xHA

Expression

Mammalian

Promoter

EF-1α

Available Since

May 31, 2018

Availability

Academic Institutions and Nonprofits only

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pYL-kasOp-FnCas12a2

Plasmid

#177874

Purpose

Expresses Streptomyces codon optimized FnCas12a. Used to modify genome of Streptomyces.

Depositor

Yunzi Luo

Article

Zhang et al Front Bioeng Biotechnol. 2020 Jun 30;8:7

Insert

Streptomyces codon optimized FnCas12a

Use

CRISPR

Promoter

kasOp*

Available Since

Jan. 13, 2022

Availability

Academic Institutions and Nonprofits only

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PB-CMV-NLS-dCas9-NLS

Plasmid

#99908

Purpose

For the expression of NLS-dCas9-NLS

Depositor

Yujie Sun

Article

Shao et al Nucleic Acids Res. 2016 May 19;44(9):e86

Insert

NLS-dCas9-NLS

Use

CRISPR

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 14, 2017

Availability

Academic Institutions and Nonprofits only

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MSP2283

Plasmid

#70702

Purpose

Bacterial expression plasmid for SaCas9 & sgRNA targeted to site 1: T7-humanSaCas9-NLS-3xFLAG-T7-Sa-sgRNA(84) #1

Depositor

Keith Joung

Article

Kleinstiver et al Nat Biotechnol. 2015 Nov 2. doi: 10.1038

Inserts

mammalian codon-optimized Staphylococcus aureus Cas9
SaCas9 sgRNA (+84)

Use

CRISPR

Tags

NLS-3xFLAG

Expression

Bacterial

Promoter

T7

Available Since

Dec. 4, 2015

Availability

Academic Institutions and Nonprofits only

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pHR-PGK- dCas9-SunTag-P2A-HygR

Plasmid

#193137

Purpose

Expresses dCas9 fused to the SunTag scaffold

Depositor

Brad Rosenberg

Article

Danziger et al PLoS Pathog. 2022 Apr 14;18(4):e1010464.

Insert

dCas9-SunTag-T2A-HygR

Use

Lentiviral

Promoter

PGK

Available Since

Nov. 10, 2022

Availability

Academic Institutions and Nonprofits only

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pORANGE GFP-Arpc5 KI

Plasmid

#131503

Purpose

Endogenous tagging of Arp2/3 complex subunit 5: C-terminal (amino acid position: STOP codon)

Depositor

Harold MacGillavry

Article

Willems et al PLoS Biol. 2020 Apr 10;18(4):e3000665. d

Insert

gRNA and GFP donor (Arpc5, Arpc5 Rat, Mouse)

Expression

Mammalian

Promoter

U6 and Cbh

Available Since

Sept. 20, 2019

Availability

Academic Institutions and Nonprofits only

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pJZC33

Plasmid

#62330

Purpose

sgRNA + 2x MS2 with MCP-VP64 effector for mammalian cells

Depositor

Wendell Lim, Stanley Qi

Article

Zalatan et al Cell. 2014 Dec 18. pii: S0092-8674(14)01

Inserts

sgRNA + 2x MS2 binding module
MCP-VP64

Use

Lentiviral

Tags

VP64

Expression

Mammalian

Mutation

Targets Tet3G, sequence: GTACGTTCTCTATCACTGATA

Promoter

CMV and U6

Available Since

April 2, 2015

Availability

Academic Institutions and Nonprofits only

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paGFP-HRas

Plasmid

#18693

Depositor

Karel Svoboda

Article

Harvey et al Science. 2008 Jul 4. 321(5885):136-40.

Insert

H-Ras (HRAS Human)

Tags

photoactivatable GFP

Expression

Mammalian

Mutation

A206K mutation in paGFP

Available Since

July 2, 2008

Availability

Academic Institutions and Nonprofits only

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pDT-SuperFi-Cas9

Plasmid

#183630

Purpose

Expression and purification of N-terminal His-MBP tagged SuperFi Cas9 in E. coli

Depositor

David Taylor

Article

Bravo et al Nature. 2022 Mar 2. pii: 10.1038/s41586-

Insert

SuperFi-Cas9

Use

CRISPR

Tags

6xHis-MBP

Expression

Bacterial

Mutation

Y1010D, Y1013D, Y1016D, V1018D, R1019D, Q1027D, …

Promoter

T7

Available Since

May 17, 2022

Availability

Academic Institutions and Nonprofits only

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pEG302 22aa SunTag VP64 g4+g17

Plasmid

#120252

Purpose

CRISPR-Cas9 SunTag system to target VP64 to the FWA locus with two guide RNAs

Depositor

Steven Jacobsen

Article

Papikian et al Nat Commun. 2019 Feb 13;10(1):729. doi:

Insert

g17_U6_g4_U6_NOS_NLS_GB1_NLS_linker_VP64_linker_sfGFP_scFv_UBQ10_Insulator_UBQ10_Ω_dCas9_1xHA_3xNLS_linker_10xGCN4_OCS

Use

CRISPR and Synthetic Biology

Expression

Plant

Available Since

March 17, 2019

Availability

Academic Institutions and Nonprofits only

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pAAV-RSV-GFP-U6-gRNA scaffold (SpyCas9)

Plasmid

#113039

Purpose

AAV vector; encodes GFP as well as a U6-driven gRNA scaffold (SpyCas9)

Depositor

Dominik Niopek

Article

Bubeck et al Nat Methods. 2018 Oct 30. pii: 10.1038/s

Insert

2x BbsI sites - SpCas9 scaffold, co-expressed GFP (transfection marker)

Use

AAV

Expression

Mammalian

Available Since

Nov. 1, 2018

Availability

Academic Institutions and Nonprofits only

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pU1/Sm/3' Box_(GLuc)_TOP1

Plasmid

#68437

Purpose

Transient expression of the "TOP1" construct, targeting the GLuc reporter, in mammalian cells. U1Pro/SmBox/3' Box expression backbone.

Depositor

John Rinn

Article

Shechner et al Nat Methods. 2015 Jul;12(7):664-70. doi:

Insert

TOP1 construct

Use

CRISPR and Synthetic Biology

Expression

Mammalian

Promoter

human U1

Available Since

Sept. 22, 2015

Availability

Academic Institutions and Nonprofits only

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pFYF1328 EGFP Site#3

Plasmid

#47513

Purpose

human gRNA expression vector targeting EGFP

Depositor

Keith Joung

Article

Fu et al Nat Biotechnol. 2013 Jun 23. doi: 10.103

Insert

gRNA-EGFP site 3

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

Aug. 29, 2013

Availability

Academic Institutions and Nonprofits only

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plRL61

Plasmid

#163633

Purpose

Optimized M. smegmatis CRISPRi plasmid; contains an L5 attP region (no L5 Integrase) that allows for integration when co-transformed with an L5 Integrase delivery plasmid (e.g. plRL19, ID #163634).

Depositor

Jeremy Rock

Article

Bosch et al Cell. 2021 Jul 16. pii: S0092-8674(21)00

Type

Empty backbone

Use

CRISPR

Expression

Bacterial

Promoter

Tet-On

Available Since

Aug. 10, 2021

Availability

Academic Institutions and Nonprofits only

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pCfB4906

Plasmid

#106150

Purpose

EasyCloneYALI system-based yeast integrative vector expressing Cas9 carrying hygromycin resistance marker, integration into Yarrowia lipolytica chromosomal location IntB, amp resistance

Depositor

Irina Borodina

Article

Holkenbrink et al Biotechnol J. 2018 Jan 29. doi: 10.1002/

Insert

Cas9

Expression

Yeast

Available Since

April 6, 2018

Availability

Academic Institutions and Nonprofits only

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pAct-Fok1:dCas9

Plasmid

#62211

Purpose

Expresses Fok1:dCas9 under control of act5C promoter and SV40 3'UTR. For ubiquitous high specificity genome engineering in Drosophila melanogaster.

Depositor

Simon Bullock

Insert

Fok1-dCas9

Expression

Insect

Promoter

act5C

Available Since

March 12, 2015

Availability

Academic Institutions and Nonprofits only

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pHD-SPARC2-D-LexA::p65

Plasmid

#133562

Purpose

Swap out effector and terminator to generate SPARC2-D CRISPR-donor vector for CRISPR-HDR genomic insertion near the attP40 locus

Depositor

Tom Clandinin

Article

Isaacman-Beck et al Nat Neurosci. 2020 Sep;23(9):1168-1175.

Insert

LexA::p65

Use

CRISPR

Expression

Insect

Promoter

20X UAS

Available Since

Oct. 31, 2019

Availability

Academic Institutions and Nonprofits only

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pHD-SPARC2-I-LexA::p65

Plasmid

#133563

Purpose

Swap out effector and terminator to generate SPARC2-I CRISPR-donor vector for CRISPR-HDR genomic insertion near the attP40 locus

Depositor

Tom Clandinin

Article

Isaacman-Beck et al Nat Neurosci. 2020 Sep;23(9):1168-1175.

Insert

LexA::p65

Use

CRISPR

Expression

Insect

Promoter

20X UAS

Available Since

Oct. 30, 2019

Availability

Academic Institutions and Nonprofits only

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pU6_(GLuc)_INT[Spinach2]

Plasmid

#68430

Purpose

Transient expression in mammalian cells of an "INT" construct_bearing the "Spinach2" aptamer, targeting the GLuc reporter. U6 promoter

Depositor

John Rinn

Article

Shechner et al Nat Methods. 2015 Jul;12(7):664-70. doi:

Insert

INT construct_bearing the "Spinach2" aptamer

Use

CRISPR and Synthetic Biology

Expression

Mammalian

Promoter

human U6

Available Since

Oct. 5, 2015

Availability

Academic Institutions and Nonprofits only

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DHFR-PP7-VP64_T2A_GFP

Plasmid

#86167

Purpose

Expresses PP7-VP64 fused to DHFR domain on N-terminus in mammalian cells

Depositor

Amit Choudhary

Article

Maji et al Nat Chem Biol. 2017 Jan;13(1):9-11. doi:

Insert

DHFR-PP7-VP64_T2A_GFP

Use

CRISPR and Lentiviral

Tags

Destabilized domain (N-terminus version) of E.col…

Expression

Mammalian

Promoter

EF1a

Available Since

Feb. 9, 2017

Availability

Academic Institutions and Nonprofits only

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