We narrowed to 10,133 results for: ada

1. pSBbi_RP_MicroID2-FLAG-NES

   Plasmid

   #188525

   Purpose

   Encodes the biotin proximity labeling enzyme MicroID2 with a nuclear export sequence (NES) for expression and stable integration into mammalian cells

   Depositor

   James Londino

   Article

   Johnson et al Mol Cell Proteomics. 2022 Jun 7:100256.

   Insert

   MicroID2
   

   Use

   Transposon mediated gene integration

   Tags

   FLAG

   Expression

   Mammalian

   Promoter

   EF1a/RPBSA

   Available Since

   Sept. 22, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. LentiCRISPR v2-sgLKB1 clone3

   Plasmid

   #162127

   Purpose

   Lentiviral sgRNA plasmid targeting human LKB1

   Depositor

   Boyi Gan

   Article

   Lee et al Nat Cell Biol. 2020 Feb;22(2):225-234. d

   Insert

   sgLKB1 (STK11 Human)
   

   Use

   Lentiviral

   Available Since

   Jan. 26, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pMito-miRFP718nano

   Plasmid

   #195754

   Purpose

   Mitochondria labelled with near-infrared fluorescent protein miRFP718nano

   Depositor

   Vladislav Verkhusha

   Article

   Oliinyk et al Nat Methods. 2022 Dec 1. doi: 10.1038/s4

   Insert

   Human cytochrome C oxidase subunit VIII-miRFP718nano
   

   Tags

   miRFP718nano

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Feb. 17, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. opto-Tiam1

   Plasmid

   #174508

   Purpose

   Expresses opto-Tiam1 (BcLOV4-GGGSx2-Tiam1DH-GGGSx2-mCherry) in a pcDNA3.1 backbone.

   Depositor

   Brian Chow

   Article

   Berlew et al ACS Synth Biol. 2022 Jan 21;11(1):515-52

   Insert

   opto-Tiam1-mCherry
   

   Tags

   mCherry

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   May 4, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pH2B-miRFP718nano

   Plasmid

   #195750

   Purpose

   Histone 2B labelled with near-infrared fluorescent protein miRFP718nano

   Depositor

   Vladislav Verkhusha

   Article

   Oliinyk et al Nat Methods. 2022 Dec 1. doi: 10.1038/s4

   Insert

   H2B-miRFP718nano
   

   Tags

   miRFP718nano

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Feb. 17, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. MAC_N_GCM1

   Plasmid

   #167759

   Purpose

   Expresses GCM1 in mammalian cells

   Depositor

   Markku Varjosalo

   Article

   Goos et al Nat Commun. 2022 Feb 9;13(1):766. doi: 1

   Insert

   GCM1 (GCM1 Human)
   

   Tags

   MAC

   Expression

   Mammalian

   Mutation

   no mutations

   Available Since

   Jan. 24, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. p8103 LentiCRISPR v2 sgNT-2

   Plasmid

   #163313

   Purpose

   Expresses Cas9 and a non-targeting control guide RNA

   Depositor

   Elizabeth White

   Article

   Hatterschide et al Proc Natl Acad Sci U S A. 2019 Apr 2;116

   Insert

   sgNT-2
   

   Use

   CRISPR and Lentiviral

   Promoter

   U6

   Available Since

   April 5, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. Yellow Cameleon-Nano50/pcDNA3

   Plasmid

   #51964

   Purpose

   mammalian expression of Yellow Cameleon-Nano50, an ultrasensitive Ca2+ indicator with Kd = 50 nM

   Depositor

   Takeharu Nagai

   Article

   Horikawa et al Nat Methods. 2010 Sep;7(9):729-32. doi:

   Insert

   Yellow Cameleon-Nano50
   

   Expression

   Mammalian

   Available Since

   March 18, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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9. qTAG-AAVS1-Ef1a-Zeo

   Plasmid

   #227268

   Purpose

   Empty AAVS1 targeting donor for the insertion of Zeo and a strong EF1a promoter. A CDS can be cloned adjacent to the promoter via restriction or gibson cloning using the MluI cut site.

   Depositor

   Laurence Pelletier

   Article

   Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

   Type

   Empty backbone

   Use

   CRISPR; Donor cassette

   Expression

   Mammalian

   Available Since

   Nov. 5, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. AAV-syn-ChR2-Kv-P2A-H2b-mRuby2

    Plasmid

    #89570

    Purpose

    Channelrhodopsin localized to neuronal soma with simultaneous nuclear labeling

    Depositor

    McLean Bolton

    Article

    Baker et al Elife. 2016 Aug 15;5. pii: e14193. doi:

    Inserts

    Channelrhodopsin
    Histone 2B
    

    Use

    AAV

    Tags

    mRuby2

    Expression

    Mammalian

    Mutation

    H134R

    Promoter

    synapsin

    Available Since

    Feb. 7, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pDV02_linear_entry_TagBFP-2A-MCS-2A-mNeonGreen

    Plasmid

    #200401

    Purpose

    Entry vector for open-loop ADAR-based RNA sensors. Expresses TagBFP constitutively. Expresses mNeonGreen upon target detection

    Depositor

    James Collins

    Article

    Gayet et al Nat Commun. 2023 Mar 11;14(1):1339. doi:

    Insert

    Open-loop DART VADAR sensor entry cassette
    

    Use

    Synthetic Biology

    Expression

    Mammalian

    Available Since

    June 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. CAV1-K*R-HA

    Plasmid

    #27765

    Depositor

    Ari Helenius

    Article

    Hayer et al J Cell Biol. 2010 Nov 1. 191(3):615-29.

    Insert

    caveolin-1, lysine null mutant (CAV1 Human)
    

    Tags

    HA

    Expression

    Mammalian

    Mutation

    All lysines mutated to arginines (K*R)

    Available Since

    Aug. 11, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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13. pHLP700

    Plasmid

    #203742

    Purpose

    C-terminal tagging of target proteins with AID*/3xHA degron

    Depositor

    Mark Hall

    Article

    Milholland et al mSphere. 2023 Aug 18:e0028323. doi: 10.1

    Insert

    IAA17 auxin binding domain codons 71-114
    

    Use

    Pcr amplification template for gene tagging

    Tags

    3xHA

    Promoter

    none

    Available Since

    Aug. 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pHLP701

    Plasmid

    #203743

    Purpose

    C-terminal tagging of target proteins with 3xV5-IAA17 degron

    Depositor

    Mark Hall

    Article

    Milholland et al mSphere. 2023 Aug 18:e0028323. doi: 10.1

    Insert

    IAA17 auxin binding domain
    

    Use

    Pcr amplification template for gene tagging

    Tags

    3xV5

    Promoter

    none

    Available Since

    Aug. 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCSIIbsr-6013x

    Plasmid

    #108654

    Purpose

    An HRas biosensor based on fluorescence resonance energy transfer (FRET) and those based on bioluminescence energy transfer (BRET)

    Depositor

    Michiyuki Matsuda

    Article

    Komatsu et al Sci Rep. 2018 Jun 12;8(1):8984. doi: 10.

    Insert

    Raichu-EV
    

    Use

    Lentiviral

    Tags

    XFP

    Expression

    Mammalian

    Available Since

    Aug. 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pUC19_dTAG-KANSL3-targeting

    Plasmid

    #170755

    Purpose

    Donor plasmid for BSD-P2A-2xHA-dTAG knock-in into the N-terminus of the mouse Kansl3 locus

    Depositor

    Kristian Helin

    Article

    Damhofer et al STAR Protoc. 2021 Jun 2;2(2):100575. doi

    Insert

    BSD-P2A-2xHA-dTAG
    

    Use

    Mouse Targeting

    Tags

    BSD-P2A-2xHA-dTAG

    Mutation

    Phenylalanine 36 to valine in FKBP12

    Available Since

    June 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pUWR-Cad-Venus

    Plasmid

    #58328

    Purpose

    Destination vector for inserting ubi-Cad-Venus to Drosophila melanogaster genome

    Depositor

    Denise Montell

    Article

    Cai et al Cell. 2014 May 22;157(5):1146-59. doi: 1

    Insert

    Cad-Venus
    

    Expression

    Insect

    Promoter

    poly-ubiquitin

    Available Since

    Aug. 14, 2014

    Availability

    Academic Institutions and Nonprofits only

18. EK0307 CMV-TO-BFPmut-stop-3UTR(Bdnf) (FLP-IN)

    Plasmid

    #191145

    Purpose

    Expression of a synthetic construct with 3' UTR of murine Bdnf in the 3' UTR of an inactive BFP in mammalian cells.

    Depositor

    Xiaojing Gao

    Article

    Kaseniit et al Nat Biotechnol. 2022 Oct 5. pii: 10.1038

    Insert

    3UTR(Bdnf)
    

    Expression

    Mammalian

    Mutation

    WT

    Promoter

    CMV-TO

    Available Since

    Oct. 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pHis2-Arf1 (17-181)-Q71L

    Plasmid

    #184808

    Purpose

    Bacterial expression of Arf1 (aa17-181, Q71L mutation)

    Depositor

    James Hurley

    Article

    Ren et al Cell. 2013 Feb 14;152(4):755-67. doi: 10

    Insert

    Arf1 (ARF1 Human)
    

    Tags

    His6

    Expression

    Bacterial

    Mutation

    aa 17-181 only, Q71L

    Promoter

    T7

    Available Since

    Oct. 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. p7274 MSCV-P C-FlagHA 16 ∆DLYC

    Plasmid

    #163304

    Purpose

    Expresses HPV16 E7 ∆DLYC

    Depositor

    Elizabeth White

    Article

    Hatterschide et al Proc Natl Acad Sci U S A. 2019 Apr 2;116

    Insert

    HPV16 E7 ∆DLYC
    

    Use

    Retroviral

    Tags

    FlagHA

    Mutation

    ∆DLYC

    Promoter

    MSCV LTR

    Available Since

    Jan. 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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