12,329 results

1. pBP-TL8

   Plasmid

   #72992

   Purpose

   RBS_L1

   Depositor

   Paul Freemont

   Article

   Moore et al ACS Synth Biol. 2016 May 2.

   Insert

   L1 RBS
   

   Use

   Synthetic Biology

   Mutation

   CAT gene - C435G (nucleotide) - silent mutagenesi…

   Available Since

   July 6, 2016

   Availability

   Academic Institutions and Nonprofits only

2. pBP-L1U1H09

   Plasmid

   #73008

   Purpose

   L1U1H09 terminator

   Depositor

   Paul Freemont

   Article

   Moore et al ACS Synth Biol. 2016 May 2.

   Insert

   L1U1H09 terminator
   

   Use

   Synthetic Biology

   Mutation

   CAT gene - C435G (nucleotide) - silent mutagenesi…

   Available Since

   July 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCru5-/CGA-mEGFP-IRES-mCherry

   Plasmid

   #49224

   Purpose

   Retroviral expression vector. Contains an altered Kozak sequence and/or upstream open reading frames to modulate expression at the level of translation.

   Depositor

   Clifford Wang

   Article

   Ferreira et al Proc Natl Acad Sci U S A. 2013 Jul 9;110

   Inserts

   mEGFP
   mCherry
   

   Use

   Retroviral and Synthetic Biology

   Tags

   SfuI site to create C-terminal fusions

   Expression

   Mammalian

   Promoter

   Viral LTR (or CMV)

   Available Since

   Dec. 5, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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4. pFTK051

   Plasmid

   #171323

   Purpose

   Part Plasmid Entry Vector (LVL0) from the Fungal Modular Cloning ToolKit.

   Depositor

   Arnold Driessen

   Article

   Mozsik et al ACS Synth Biol. 2021 Nov 2. doi: 10.1021

   Insert

   eBFP fluorescent reporter
   

   Use

   Synthetic Biology

   Expression

   Bacterial and Yeast

   Mutation

   n/a

   Available Since

   Jan. 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. Construct 3 - U6p_20nt1_mod2_sgRNA (stgRNA)

   Plasmid

   #81246

   Purpose

   For assaying self-targeting activity via Cas9

   Depositor

   Timothy Lu

   Article

   Perli et al Science. 2016 Aug 18. pii: aag0511.

   Insert

   sgRNA
   

   Use

   Lentiviral and Synthetic Biology

   Expression

   Mammalian

   Available Since

   Sept. 14, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. pBdxs

   Plasmid

   #17821

   Depositor

   Jay Keasling

   Article

   Kim et al Biotechnol Bioeng. 2001 Feb 20. 72(4):40

   Insert

   dxs
   

   Use

   Synthetic Biology

   Expression

   Bacterial

   Available Since

   May 14, 2008

   Availability

   Academic Institutions and Nonprofits only

7. dCas9-3×RFP-P2A-CASANOVA/gRNA telo

   Plasmid

   #137711

   Purpose

   Vector co-expressing dCas9-3×RFP-P2A-CASANOVA and sgRNA telo

   Depositor

   Dominik Niopek

   Insert

   dCas9-3×RFP-P2A-CASANOVA/gRNA telo
   

   Use

   CRISPR, Lentiviral, and Synthetic Biology

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   March 4, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pBBRBB-Ppuf1-1200-DsRed

   Plasmid

   #50960

   Purpose

   Expressed DsRed using the Ppuf1-1200 promoter segment, for use in R. sphaeroides

   Depositor

   Claudia Schmidt-dannert

   Article

   Tikh et al Appl Microbiol Biotechnol. 2014 Feb 9.

   Insert

   DsRed
   

   Use

   Synthetic Biology

   Promoter

   Ppuf1-1200

   Available Since

   March 5, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCG-A-sacB(SP)-B

   Plasmid

   #87370

   Purpose

   An ORF part-containing vector harbouring the signal peptide from SacB with 4 bp overhangs specific for the ‘signal peptide’ position within an ORF assembly

   Depositor

   Tom Ellis

   Article

   Gilbert et al ACS Synth Biol. 2017 Mar 7. doi: 10.1021

   Insert

   SacB signal peptide
   

   Use

   Synthetic Biology

   Available Since

   July 21, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCG-B-SpyTag-C

    Plasmid

    #87373

    Purpose

    An ORF part-containing vector harbouring the SpyTag sequence with 4 bp overhangs specific for the ‘upstream SpyPart’ position within an ORF assembly

    Depositor

    Tom Ellis

    Article

    Gilbert et al ACS Synth Biol. 2017 Mar 7. doi: 10.1021

    Insert

    SpyTag
    

    Use

    Synthetic Biology

    Available Since

    July 21, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. pBbS4a-RFP

    Plasmid

    #35301

    Depositor

    Jay Keasling

    Article

    Lee et al J Biol Eng. 2011 Sep 20;5:12.

    Insert

    RFP
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Promoter

    ProE

    Available Since

    Feb. 17, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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12. pHR-PGK-LexA-CIB1-biLINuS-P2A-tagBFP

    Plasmid

    #192609

    Purpose

    Lentiviral vector for constitutive expression of LexA-CIB1-biLINuS-P2A-tagBFP.

    Depositor

    Yingxiao Wang

    Article

    Huang et al Sci Adv. 2020 Feb 19;6(8):eaay9209. doi:

    Insert

    LexA-CIB1-biLINuS-P2A-tagBFP
    

    Use

    Lentiviral and Synthetic Biology

    Tags

    tagBFP

    Promoter

    PGK

    Available Since

    Nov. 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. C413-E22: attB4-polyhedrinp>-attB1r

    Plasmid

    #162922

    Purpose

    Gateway attB4/attB1r entry clone for baculovirus expression using the AcMNPV polyhedrin promoter

    Depositor

    Dominic Esposito

    Article

    Wall et al Methods Mol Biol. 2014;1116:193-208. doi

    Insert

    AcMNPV polyhedrin promoter
    

    Use

    Synthetic Biology

    Available Since

    April 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pBDKRB2_TCS(Q’L)_HA-dCas9(N)_P2A-Puro-WPRE

    Plasmid

    #101108

    Purpose

    Encodes dCas9(N) fused to BDKRB2

    Depositor

    Tudor Fulga

    Article

    Baeumler et al Cell Rep. 2017 Sep 12;20(11):2639-2653.

    Insert

    BDKRB2-dCas9(N)
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Flag and HA

    Expression

    Mammalian

    Available Since

    Nov. 13, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. pEF 4r-GFPGUS-3r

    Plasmid

    #20098

    Depositor

    Antonio Richart

    Insert

    GFPGUS
    

    Use

    Synthetic Biology; Plant gateway vector

    Available Since

    March 11, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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16. pBW2065_pCAG-PV1-GID1-L1-CTD-ENTRY-BGHpA

    Plasmid

    #108843

    Purpose

    Insert protein fragment into KpnI/EcoRI to fuse to GID1 tag on N-terminus

    Depositor

    Wilson Wong

    Article

    Weinberg et al Nat Commun. 2019 Oct 24;10(1):4845. doi:

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    GID1

    Expression

    Mammalian

    Available Since

    Aug. 28, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. pY1

    Plasmid

    #50597

    Purpose

    Backbone: pRBS-01, Origin Of Replication: p15a, Selection Markers: Amp, Promoters: PLacUV5,

    Depositor

    Jay Keasling

    Article

    Juminaga et al Appl Environ Microbiol. 2012 Jan;78(1):8

    Type

    Empty backbone

    Use

    Synthetic Biology; Empty vector

    Expression

    Bacterial

    Promoter

    PLacUV5

    Available Since

    Oct. 21, 2014

    Availability

    Academic Institutions and Nonprofits only

18. pU6_(GLuc)_INT[P4-P6(3xPP7)]

    Plasmid

    #68432

    Purpose

    Transient expression in mammalian cells of an "INT" construct_bearing P4_P6 (internally appended with three PP7 stem-loops), targeting the GLuc reporter. U6 promoter

    Depositor

    John Rinn

    Article

    Shechner et al Nat Methods. 2015 Jul;12(7):664-70. doi:

    Insert

    INT construct_bearing P4_P6 (internally appended with three PP7 stem-loops)
    

    Use

    CRISPR and Synthetic Biology

    Expression

    Mammalian

    Promoter

    human U6

    Available Since

    Oct. 5, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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19. pDUET-ctCPR-nAMO

    Plasmid

    #20110

    Depositor

    Jay Keasling

    Article

    Chang et al Nat Chem Biol. 2007 May . 3(5):274-7.

    Insert

    p450 reductase and native Amorphadiene oxidase
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Available Since

    Feb. 27, 2009

    Availability

    Academic Institutions and Nonprofits only

20. pPSP6+t-gfp

    Plasmid

    #48130

    Purpose

    Shuttle vector E. coli/B.meg.; gfp-expression under control of RNAP-SP6-inducible promoter

    Depositor

    Dieter Jahn

    Article

    Stammen et al Appl Microbiol Biotechnol. 2010 Sep;88(2

    Insert

    gfp
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Available Since

    Oct. 21, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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