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plenti.CamKII.(cyto).iATPSnFR2.A95K.S29W.miRFP670nano3

Plasmid

#209728

Purpose

Expresses cytosolic high affinity ratiometric ATP sensor

Depositor

Timothy Ryan

Article

Marvin et al Proc Natl Acad Sci U S A. 2024 May 21;12

Insert

(cyto).iATPSnFR2.A95K.S29W.miRFP670nano3

Use

Lentiviral

Expression

Mammalian

Promoter

CamKII

Available Since

Oct. 30, 2024

Availability

Academic Institutions and Nonprofits only

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qTAG-C-mStayGold-EFS-Blast

Plasmid

#227257

Purpose

Empty C-terminus donor cassette. Integrate homology arms to target the C-terminus insertion of a mStayGold-EFS-Blast cassette

Depositor

Laurence Pelletier

Article

Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

Type

Empty backbone

Use

CRISPR; Donor cassette

Expression

Mammalian

Available Since

Nov. 12, 2024

Availability

Academic Institutions and Nonprofits only

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Cas9 TLS2

Plasmid

#196978

Purpose

Expresses Cas9 fused with TLS2 mobility signal that confers graft mobility. Induced by estradiol.

Depositor

Friedrich Kragler

Article

Yang et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

Insert

Cas9 with TLS2 tag

Expression

Plant

Promoter

pG10-90

Available Since

May 12, 2023

Availability

Academic Institutions and Nonprofits only

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pLenti-mCherry-CAAX

Plasmid

#129285

Purpose

Lentiviral plasmid for expression of fluorescent reporter mCherry targeted to the plasma membrane via CAAX polybasic sequence

Depositor

Carlos Carmona-Fontaine

Article

Janska et al Dis Model Mech. 2021 Aug 1;14(8). pii: 2

Insert

Fluorescent reporter mCherry fused to CAAX polybasic sequence

Use

Lentiviral

Tags

CAAX

Expression

Mammalian

Promoter

CMV promoter

Available Since

June 22, 2022

Availability

Academic Institutions and Nonprofits only

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pCRISPR_eGFP_497

Plasmid

#111824

Purpose

Knockout eGFP

Depositor

Steve Shih

Article

Sinha et al Lab Chip. 2018 Jul 24;18(15):2300-2312.

Insert

gRNA targeting eGFP 477-497

Use

CRISPR

Tags

mCherry

Expression

Bacterial and Mammalian

Promoter

U6

Available Since

Aug. 2, 2018

Availability

Academic Institutions and Nonprofits only

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pAAV.CAG.(cyto).iATPSnFR2.A95K.tdTomato

Plasmid

#248072

Purpose

iATPSnFR2.A95K variant with non-responsive tdTomato

Depositor

Tim Brown

Article

Marvin et al Proc Natl Acad Sci U S A. 2024 May 21;12

Insert

iATPSnFR2.A95K.tdTomato

Use

AAV

Mutation

A95K

Available Since

Jan. 8, 2026

Availability

Academic Institutions and Nonprofits only

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plenti.CMV.(cyto).iATPSnFR2.A95A.A119L.miRFP670nano3

Plasmid

#236407

Purpose

Expresses cytosolic low affinity ratiometric ATP sensor

Depositor

Timothy Ryan

Article

Marvin et al Proc Natl Acad Sci U S A. 2024 May 21;12

Insert

(cyto).iATPSnFR2.A95A.A119L.miRFP670nano3

Use

Lentiviral

Expression

Mammalian

Promoter

CMV

Available Since

April 24, 2025

Availability

Academic Institutions and Nonprofits only

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pCI-neo Fluc EGFP

Plasmid

#90170

Purpose

EGFP-tagged constructs to monitor the aggregation state of the sensors and the ability of cells to solubilize or degrade the aggregated proteins

Depositor

Franz-Ulrich Hartl

Article

Gupta et al Nat Methods. 2011 Sep 4;8(10):879-84. do

Insert

FLuc EGFP

Expression

Mammalian

Available Since

Aug. 4, 2017

Availability

Academic Institutions and Nonprofits only

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pTRIP-CMV-Puro-2A

Plasmid

#102611

Purpose

Control lentivector expressing Puro resistance and gene of interest from CMV promoter

Depositor

Nicolas Manel

Article

Gentili et al Science. 2015 Sep 11;349(6253):1232-6. d

Type

Empty backbone

Use

Lentiviral

Expression

Mammalian

Available Since

Jan. 19, 2018

Availability

Academic Institutions and Nonprofits only

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EGFP-Talin head-SpyCatcher003

Plasmid

#133566

Purpose

Expresses EGFP-Talin head-SpyCatcher003 in mammalian cells

Depositor

Mark Howarth

Article

Keeble et al Proc Natl Acad Sci U S A. 2019 Dec 10. p

Insert

EGFP-Talin head-SpyCatcher003

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 19, 2019

Availability

Academic Institutions and Nonprofits only

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pGL3-Basic-IRES

Plasmid

#64784

Purpose

Luciferase reporter vector

Depositor

Joshua Mendell

Article

Chang et al Mol Cell. 2007 Jun 8;26(5):745-52. Epub

Type

Empty backbone

Use

Luciferase

Available Since

July 17, 2015

Availability

Academic Institutions and Nonprofits only

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pTfR-PAmKate

Plasmid

#32692

Depositor

Vladislav Verkhusha

Article

Gunewardene et al Biophys J. 2011 Sep 21;101(6):1522-8. Ep

Insert

PAmKate

Tags

transferrin receptor (TfR)

Expression

Mammalian

Mutation

F84W/S148N/S165G compared to mKate (but numbering…

Promoter

CMV

Available Since

Oct. 21, 2011

Availability

Academic Institutions and Nonprofits only

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pCI-neo FlucSM EGFP

Plasmid

#90171

Purpose

EGFP-tagged constructs to monitor the aggregation state of the sensors and the ability of cells to solubilize or degrade the aggregated proteins. FLuc contains single mutation

Depositor

Franz-Ulrich Hartl

Article

Gupta et al Nat Methods. 2011 Sep 4;8(10):879-84. do

Insert

FLuc EGFP

Expression

Mammalian

Mutation

R188Q in FLuc

Available Since

Aug. 4, 2017

Availability

Academic Institutions and Nonprofits only

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pPig_8X-TOPFLash-tdIRFP_Puro

Plasmid

#249713

Purpose

TopFlash reporter with puromycin. Used as a live readout of downstream Wnt gene transcription.

Depositor

Max Wilson

Article

Rosen et al bioRxiv [Preprint]. 2025 May 23:2025.02.

Inserts

tdiRFP
T2A-PuroR

Expression

Mammalian

Promoter

TopFlash and TopFlash, with 7x TCF/LEF motif

Available Since

Jan. 14, 2026

Availability

Academic Institutions and Nonprofits only

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pcDNA5-FRT-TO-eGFP

Plasmid

#157733

Purpose

mammalian expression of untagged eGFP under control of a tetracycline-inducible promoter

Depositor

David Tollervey

Article

Bresson et al Wellcome Open Res. 2020 Nov 3;5:261. doi

Insert

eGFP

Expression

Mammalian

Available Since

Aug. 20, 2020

Availability

Academic Institutions and Nonprofits only

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MXS_CMV::rtTA3-bGHpA

Plasmid

#62445

Purpose

MXS_chaining vector with CMV::rtTA3-bGHpA

Depositor

Pierre Neveu

Article

Sladitschek et al PLoS One. 2015 Apr 24;10(4):e0124958. do

Insert

cassette containing the reverse tetracycline transactivator with CMV promoter

Use

Synthetic Biology

Available Since

May 8, 2015

Availability

Academic Institutions and Nonprofits only

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p415-GalL-Cas9-CYC1t

Plasmid

#43804

Purpose

A human codon-optimized Cas9 for expression in S. cerevisiae (budding yeast) from the GalL promoter

Depositor

George Church

Article

Dicarlo et al Nucleic Acids Res

Insert

Human Optimized S. pyogenes Cas9

Use

CRISPR

Expression

Yeast

Promoter

GalL

Available Since

March 15, 2013

Availability

Academic Institutions and Nonprofits only

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pCMV-Tag2B-NCre

Plasmid

#106368

Purpose

N-terminal part of the split-Cre System

Depositor

Johannes Hirrlinger

Article

Hirrlinger et al PLoS One. 2009;4(1):e4286. doi: 10.1371/

Insert

NCre

Use

Cre/Lox

Tags

flag

Expression

Mammalian

Promoter

CMV

Available Since

March 20, 2018

Availability

Academic Institutions and Nonprofits only

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pdCas9-Tet1-CD

Plasmid

#83340

Purpose

To introduce dCas9-fused Tet1-CD and sgRNA2.0 system

Depositor

Ronggui Hu

Article

Xu et al Cell Discov. 2016 May 3;2:16009. doi: 10

Insert

dCas9-Tet1-CD and sgRNA scaffold with 2xMS2 binding sites

Use

CRISPR

Expression

Mammalian

Mutation

D10A, H840A

Promoter

U6, CBH

Available Since

Oct. 17, 2016

Availability

Academic Institutions and Nonprofits only

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pPEG

Plasmid

#162466

Purpose

Entry vector for gRNA insertion in plant prime editing expression vector

Depositor

Adam Bogdanove

Article

Wang et al Front Genome Ed. 2021 Mar 4;3:617553. do

Type

Empty backbone

Use

Entry vector for grna

Promoter

CmYLCV

Available Since

Feb. 12, 2021

Availability

Academic Institutions and Nonprofits only

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