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PMP34-mCherry-EGFP-Tet-On

Plasmid

#109017

Purpose

Tet-On construct to induce expression of tandem mCherry-eGFP tagged PMP34 (peroxisome marker)

Depositor

Jacob Corn

Article

Liang et al J Cell Biol. 2018 Aug 24. pii: jcb.20180

Insert

PMP34 (SLC25A17 Human)

Use

Lentiviral

Tags

mCherry-eGFP

Expression

Mammalian

Promoter

CMV promoter

Available Since

Aug. 22, 2018

Availability

Academic Institutions and Nonprofits only

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138-dCas9-Dnmt3a

Plasmid

#84570

Purpose

PiggyBac transposon system construct with dCas9-Dnmt3a

Depositor

Rudolf Jaenisch

Article

Liu et al Cell. 2016 Sep 22;167(1):233-247.e17. do

Insert

dCas9-Dnmt3a (DNMT3A Synthetic, Human)

Expression

Mammalian

Available Since

Jan. 25, 2017

Availability

Academic Institutions and Nonprofits only

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lenti CAG-FLAG-dCas9-VPR

Plasmid

#114197

Purpose

Lentivirus compatible dCas9-VPR construct driven by the CAG promoter

Depositor

Jeremy Day

Article

Savell et al eNeuro. 2019 Mar 7;6(1). pii: eN-MNT-049

Insert

dCas9-VPR

Use

Lentiviral

Tags

FLAG

Expression

Mammalian

Promoter

CAG

Available Since

Sept. 10, 2018

Availability

Academic Institutions and Nonprofits only

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DsRed-Rab5 DN

Plasmid

#13051

Depositor

Richard Pagano

Article

Sharma et al J Biol Chem. 2003 Feb 28. 278(9):7564-72

Insert

RAB5A (Canis familiaris) (RAB5A Canis familiaris)

Tags

DsRed2 and HA

Expression

Mammalian

Mutation

asparagine 3 changed to serine (neutral); aspara…

Available Since

Oct. 16, 2006

Availability

Academic Institutions and Nonprofits only

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pDRESS_mTurquoise2_spatial-linker-P2A_mScarlet-I3

Plasmid

#189755

Purpose

Produces an mTurquoise-antiFRET-mScarlet-I3 fusion protein in bacteria and in mamallian cells it produces 1:1 unfused mTurquoise2 and mScarlet-I3 FPs for ratiometric analysis

Depositor

Dorus Gadella

Article

Gadella TWJ et al Nat Methods. 2023 Apr;20(4):541-545. doi

Insert

mTurquoise2_spatial-linker-P2A_mScarlet-I3

Tags

6xHis

Expression

Bacterial and Mammalian

Promoter

CMV & Rha

Available Since

March 21, 2023

Availability

Academic Institutions and Nonprofits only

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CibN

Plasmid

#209671

Purpose

Expresses CIB1 fused with Split-TurboID-N and pmGFP in mammalian cells

Depositor

Sanjeevi Sivasankar

Article

Shafraz et al J Cell Sci. 2023 Oct 1;136(19):jcs261430

Insert

CIB1 (CIB1 Mustard Weed)

Tags

Split-TurboID-N and pmEGFP

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 28, 2023

Availability

Academic Institutions and Nonprofits only

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pEF5B-FRT-AP-Sstr3-GFP-DEST

Plasmid

#49098

Purpose

Expressing AP-Sstr3-GFP in mammalian cells, can be used for establishing Flp-In stable cell lines

Depositor

Maxence Nachury

Article

Ye et al Elife. 2013 Aug 6;2:e00654. doi: 10.7554

Insert

Sstr3 (Sstr3 Mouse)

Tags

GFP and acceptor peptide (AP)

Expression

Mammalian

Promoter

T7

Available Since

Nov. 8, 2013

Availability

Academic Institutions and Nonprofits only

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Dominant negative Hsp90 beta

Plasmid

#22480

Depositor

William Sessa

Article

Miao et al Arterioscler Thromb Vasc Biol. 2008 Jan

Insert

Dominant negative Hsp90 (HSP90AB1 Human)

Tags

HA

Expression

Mammalian

Available Since

Dec. 8, 2009

Availability

Academic Institutions and Nonprofits only

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PHB2-mCherry

Plasmid

#232785

Purpose

Encodes an mCherry-tagged Prohibitin2/PHB2

Depositor

Giulia Bertolin

Article

Bertolin et al Life Sci Alliance. 2021 Apr 5;4(6):e2020

Insert

PHB2 (PHB2 Human)

Tags

mCherry

Expression

Mammalian

Promoter

CMV

Available Since

March 14, 2025

Availability

Academic Institutions and Nonprofits only

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pX330a dCas9-KRAB

Plasmid

#92361

Purpose

Modified CAG promoter-containing vector for ubiquitous expression of catalytically inactive Cas9 fused to KRAB repressor domain. For targeted enhancer inactivation in chicken embryos.

Depositor

Tatjana Sauka-Spengler

Article

Williams et al Development. 2018 Feb 23;145(4). pii: de

Insert

dCas9-KRAB

Use

CRISPR

Expression

Mammalian

Promoter

CAG

Available Since

Aug. 24, 2017

Availability

Academic Institutions and Nonprofits only

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pMDC32B-AtMIR390a-B/c

Plasmid

#51776

Purpose

Plant expression vector (2x35S) for direct cloning of artificial microRNAs into Arabidopsis thaliana MIR390a precursor

Depositor

James Carrington

Article

Carbonell et al Plant Physiol. 2014 Mar 19.

Insert

AtMIR390a-B/c

Use

Plant expression

Mutation

A. thaliana MIR390a precursor sequence including …

Available Since

April 1, 2014

Availability

Academic Institutions and Nonprofits only

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pET42b-ABE7.10

Plasmid

#120398

Purpose

Expresses ABE7.10-NLS with an N-terminal His Tag (His6) for bacterial expression

Depositor

Junjiu Huang

Article

Liang et al Nat Commun. 2019 Jan 8;10(1):67. doi: 10

Insert

ABE7.10

Tags

His

Expression

Bacterial

Mutation

Mammalian codon-optimized

Promoter

T7

Available Since

April 24, 2019

Availability

Academic Institutions and Nonprofits only

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pEF5B-FRT-control-APEX

Plasmid

#73187

Purpose

Expresses "control"-APEX (cytosolic) in mammalian cells

Depositor

Maxence Nachury

Article

Mick et al Dev Cell. 2015 Nov 23;35(4):497-512. doi

Insert

control-APEX (Nphp3 Glycine max, Mouse)

Tags

internal GFP tag

Expression

Mammalian

Mutation

changed glycine 2 to alanine

Promoter

EF1alpha

Available Since

March 7, 2016

Availability

Academic Institutions and Nonprofits only

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EGFP-CLC

Plasmid

#193559

Purpose

Expresses human Clathrin Light Chain B tagged with mEGFP in mammalian cells.

Depositor

Justin Taraska

Article

Obashi et al Nat Commun. 2023 Feb 9;14(1):732. doi: 1

Insert

Clathrin light chain B (CLTB Human)

Tags

mEGFP

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 10, 2023

Availability

Academic Institutions and Nonprofits only

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pTE4396

Plasmid

#74041

Purpose

Expresses human codon-optimized AsCpf1 and As crRNA.

Depositor

Ervin Welker

Article

Toth et al Biol Direct. 2016 Sep 14;11:46. doi: 10.

Inserts

As crRNA
AsCpf1

Use

CRISPR

Tags

3xHA and NLS

Expression

Mammalian

Promoter

CMV and human U6

Available Since

April 21, 2016

Availability

Academic Institutions and Nonprofits only

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pLenti-sgRNA-lib 2.0

Plasmid

#89638

Purpose

sgRNA expression in mammalian cells after Gateway cloning

Depositor

Wensheng Wei

Article

Zhu et al Nat Biotechnol. 2016 Dec;34(12):1279-128

Type

Empty backbone

Use

Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

Feb. 16, 2018

Availability

Academic Institutions and Nonprofits only

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pCCL-MUC5ACPromoter-FLuc-CMV-RLuc-dsRed2

Plasmid

#215327

Purpose

Lentiviral mammalian vector with firefly luciferase reporter for MUC5AC expression; renilla luciferase and dsRed2 reporter under CMV promoter.

Depositor

Rob Hynds

Article

Orr et al Am J Physiol Lung Cell Mol Physiol. 2024

Insert

MUC5AC Promoter

Use

Lentiviral and Luciferase

Expression

Mammalian

Promoter

MUC5AC

Available Since

July 25, 2024

Availability

Academic Institutions and Nonprofits only

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pcU6_3 sgRNA

Plasmid

#92359

Purpose

Chicken-specific U6 sgRNA expression mini-vector, harbouring chick U6_3 pol III promoter.

Depositor

Tatjana Sauka-Spengler

Article

Williams et al Development. 2018 Feb 23;145(4). pii: de

Insert

chick U6.3 promoter and gRNA cloning cassette

Use

CRISPR

Expression

Mammalian

Promoter

chick U6.3

Available Since

Aug. 24, 2017

Availability

Academic Institutions and Nonprofits only

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pEGFPC3-hGli3

Plasmid

#65435

Purpose

Expresses human Gli3 protein in mammalian cells

Depositor

Aimin Liu

Article

Zeng et al PLoS One. 2010 Dec 29;5(12):e15900. doi:

Insert

Gli3 (GLI3 Human)

Tags

EGFP

Expression

Mammalian

Available Since

June 2, 2015

Availability

Academic Institutions and Nonprofits only
CLC-EGFP

Plasmid

#193566

Purpose

Expresses human Clathrin Light Chain B tagged with mEGFP in mammalian cells.

Depositor

Justin Taraska

Article

Obashi et al Nat Commun. 2023 Feb 9;14(1):732. doi: 1

Insert

Clathrin light chain B (CLTB Human)

Tags

mEGFP

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 10, 2023

Availability

Academic Institutions and Nonprofits only

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