59,540 results

1. pDONR221 CARD8 Ub-S297

   Plasmid

   #169965

   Purpose

   pDONR221 for Gateway shuttling. Contains the CARD8 C-terminus fused to ubiquitin (co-translationally processed)

   Depositor

   Daniel Bachovchin

   Article

   Sharif et al Immunity. 2021 Jul 13;54(7):1392-1404.e1

   Insert

   CARD8 (CARD8 Human)
   

   Use

   Gateway cloning vector

   Tags

   Ub

   Available Since

   May 12, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. PKNOX1_pCSdest

   Plasmid

   #53899

   Depositor

   Roger Reeves

   Article

   Edie et al G3 (Bethesda). 2018 Jul 2;8(7):2215-2223

   Insert

   PKNOX1 (PKNOX1 Human)
   

   Expression

   Mammalian

   Promoter

   SP6 and CMV

   Available Since

   Sept. 8, 2014

   Availability

   Industry, Academic Institutions, and Nonprofits

3. pEGFP-C3-Sec3

   Plasmid

   #53755

   Purpose

   pEGFP-C3 plasmid that expresses Sec3

   Depositor

   Channing Der

   Article

   Martin et al Mol Cell. 2014 Jan 23;53(2):209-20. doi:

   Insert

   Sec3 (EXOC1 Human)
   

   Tags

   GFP

   Expression

   Mammalian

   Available Since

   July 22, 2014

   Availability

   Academic Institutions and Nonprofits only

4. mTagBFP2-PCNA-19-SV40NLS-4

   Plasmid

   #55320

   Purpose

   Localization: Nucleus, Excitation: 399, Emission: 456

   Depositor

   Michael Davidson

   Article

   Subach et al PLoS One. 2011;6(12):e28674. Epub 2011 D

   Insert

   PCNA-19 SV40NLS (PCNA Human)
   

   Tags

   3xSV40 NLS and mTagBFP2

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Oct. 22, 2014

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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5. pFB2D

   Plasmid

   #195286

   Purpose

   Bicistronic DTR-EGFP fusion and Puromycin resistance for positive/negative selection

   Depositor

   Scott Lowe

   Article

   Barriga et al Nat Cancer. 2022 Nov;3(11):1367-1385. do

   Insert

   DTR EGFP 2A Puro
   

   Expression

   Mammalian

   Promoter

   EFS

   Available Since

   Jan. 26, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCH1/RAD51o

   Plasmid

   #102562

   Purpose

   Express Human RAD51 protein codon optimzed for E.coli and the E.coli GorE Operon

   Depositor

   Maria Spies

   Article

   Subramanyam et al Proc Natl Acad Sci U S A. 2016 Oct 11;11

   Inserts

   Homo sapiens RAD51 homolog (RecA homolog, E. coli) (S. cerevisiae) (RAD51), transcript variant 1 (RAD51 Human, Synthetic)
   E. coli groE operon
   

   Expression

   Bacterial

   Mutation

   Codon optimized for E.coli Expression

   Promoter

   T7

   Available Since

   Feb. 7, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pET11b-MIF

   Plasmid

   #149446

   Purpose

   Expression of WT MIF in E. coli for protein production

   Depositor

   Elias Lolis

   Insert

   MIF (MIF Human)
   

   Expression

   Bacterial

   Mutation

   WT

   Available Since

   Sept. 21, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pcDNA3.1-SC35-cMyc

   Plasmid

   #44721

   Depositor

   Kathleen Scotto

   Article

   Shi et al Mol Cell Biol. 2008 Jan;28(2):883-95. Ep

   Insert

   SC35 (SRSF2 Human)
   

   Tags

   his and myc

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   April 29, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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9. pcDNA3.1-FLAG-Claspin

   Plasmid

   #12659

   Depositor

   Michele Pagano

   Article

   Peschiaroli et al Mol Cell. 2006 Aug 4. 23(3):319-29.

   Insert

   Claspin (CLSPN Human)
   

   Tags

   Flag

   Expression

   Mammalian

   Available Since

   May 16, 2007

   Availability

   Academic Institutions and Nonprofits only

10. mCherry-Rab5DN(S34N)

    Plasmid

    #35139

    Depositor

    Sergio Grinstein

    Article

    Bohdanowicz et al Mol Biol Cell. 2012 Jan;23(1):176-87. Ep

    Insert

    Rab5a (RAB5A Human)
    

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Dominant Negative- S34N

    Promoter

    CMV

    Available Since

    March 20, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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11. SETD2(SET)-Cas9

    Plasmid

    #186700

    Purpose

    Plasmid encoding SETD2-Cas9 fusion under CMV promoter

    Depositor

    Jennifer Doudna

    Article

    Chen et al Proc Natl Acad Sci U S A. 2022 Dec 6;119

    Insert

    SETD2(SET)-Cas9
    

    Use

    CRISPR

    Tags

    FLAG

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    July 5, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pODN-mNG-ANLN

    Plasmid

    #183834

    Purpose

    Repair template for the N-terminal tagging of anillin with mNeonGreen in human cells using CRISPR/Cas9.

    Depositor

    Alisa Piekny

    Article

    Husser et al Open Biol. 2022 Nov;12(11):220247. doi:

    Insert

    ANLN homology arms with mNeonGreen-linker (ANLN Human)
    

    Use

    CRISPR; Donor template

    Tags

    mNeonGreen-linker

    Expression

    Mammalian

    Mutation

    Homology arms contain point mutations to remove t…

    Available Since

    May 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pAAV_Ef1a_DIO_hTyrHA_minBack

    Plasmid

    #205286

    Purpose

    Induces NM-like pigment accumulation in catecholaminergic neurons

    Depositor

    Matthias Prigge

    Insert

    hTyrosinase
    

    Use

    AAV

    Available Since

    Dec. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. TMED10-bio-His

    Plasmid

    #51852

    Purpose

    Expresses full-length Transmembrane emp24 domain-containing protein precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.

    Depositor

    Gavin Wright

    Article

    Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m

    Insert

    TMED10 (TMED10 Human)
    

    Tags

    His tag, enzymatic biotinylation sequence, and ra…

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 27, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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15. pRSET-4xTR-TUBE

    Plasmid

    #110312

    Purpose

    E. coli expression and purification of 4xTR-TUBE

    Depositor

    Yasushi Saeki

    Article

    Tsuchiya et al Nat Commun. 2018 Feb 6;9(1):524. doi: 10

    Insert

    4xTR-TUBE (UBQLN1 Human)
    

    Tags

    N-terminal His6-T7 tag

    Expression

    Bacterial

    Mutation

    All Arg residues in the UBA domain are mutated to…

    Promoter

    T7

    Available Since

    May 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pmTurquoise2-LAMP1

    Plasmid

    #110948

    Purpose

    Expresses LAMP1 fused to mTurquoise2

    Depositor

    Pablo Rivera-Fuentes

    Article

    Halabi et al Nat Commun. 2019 Mar 15;10(1):1232. doi:

    Insert

    Lysosomal-associated membrane protein 1 (LAMP1 Human)
    

    Tags

    mTurquoise2

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    March 29, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. pDEST26-PPP2CB-C-HA

    Plasmid

    #195179

    Purpose

    Vector for transient expression of PPP2CB gene with C-terminal HA tag, gene flanked by att sites for gateway cloning

    Depositor

    Stephanie Roessler

    Insert

    PPP2CB (PPP2CB Human)
    

    Tags

    HA

    Expression

    Mammalian

    Promoter

    CMV promoter

    Available Since

    Feb. 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. TFORF3124

    Plasmid

    #144600

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    SMAD3 (SMAD3 Human)
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mammalian

    Promoter

    EF1a

    Available Since

    Jan. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. CLK1

    Plasmid

    #155458

    Purpose

    For use in RBP tethering screen

    Depositor

    Eugene Yeo

    Article

    Luo et al Nat Struct Mol Biol. 2020 Oct;27(10):989

    Insert

    CLK1 (CLK1 Human)
    

    Tags

    V5-MCP

    Expression

    Mammalian

    Mutation

    none

    Promoter

    EF-1a

    Available Since

    Oct. 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. pOT_6 - lenti-EFS-FMC6.3-BBz-2A-puro-2A-tNGFR

    Plasmid

    #181975

    Purpose

    Expresses anti-CD19 CAR (with 4-1BB signaling domain) linked to puromycin resistance via P2A and to human truncated NGFR via T2A for lentiviral delivery.

    Depositor

    Neville Sanjana

    Article

    Legut et al Nature. 2022 Mar;603(7902):728-735. doi:

    Insert

    FMC6.3-BBz CAR; tNGFR (NGFR Human, Synthetic)
    

    Use

    Lentiviral

    Mutation

    NGFR truncation: aa 11-276

    Available Since

    April 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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