We narrowed to 9,645 results for: SUB

1. PX458_3XHA_den1FnCas9_KRAB_T2A_eGFP

   Plasmid

   #200910

   Purpose

   3XHA tagged dead en1Cas9 from F. novicida with KRAB repressor domain, T2A self-cleaving peptide and enhanced GFP biomarker for mammalian expression

   Depositor

   Debojyoti Chakraborty

   Insert

   3XHA_den1FnCas9_KRAB_eGFP
   

   Use

   CRISPR

   Tags

   3xHA-SV40NLS and Nucleoplasmin NLS-KRAB-T2A-EGFP

   Expression

   Mammalian

   Mutation

   Changed Glutamic Acid to Arginine at 1369 position

   Promoter

   CBH

   Available Since

   Sept. 11, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pGG-NES-TurboID-GFP

   Plasmid

   #209401

   Purpose

   Transiently expressing and visualizing the subcellular localization of TurboID fused with a nuclear export signal (NES) in planta

   Depositor

   Rosa Lozano-Durán

   Article

   Tan et al J Integr Plant Biol. 2024 Jan 31. doi: 1

   Insert

   NES-3XHA-TurboID
   

   Tags

   GFP

   Expression

   Plant

   Mutation

   TurboID gene sequence C249A

   Promoter

   Cauliflower mosaic virus (CaMV) 35S promoter

   Available Since

   Jan. 5, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. dCas9-CRY2Clust-FUSN-mut

   Plasmid

   #200970

   Purpose

   induction of phase-separated condensate mediated by FUSN-IDR mutant

   Depositor

   Jian Xu

   Article

   Kim et al Sci Adv. 2023 Mar 31;9(13):eadg1123. doi

   Insert

   FUSNmut
   

   Use

   Lentiviral

   Expression

   Bacterial and Mammalian

   Mutation

   15 Tyr-to-Ser substitutions

   Available Since

   May 22, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. IRAK1mut-UTR

   Plasmid

   #15096

   Depositor

   David Baltimore

   Article

   Taganov et al Proc Natl Acad Sci U S A. 2006 Aug 15. 1

   Insert

   IRAK1 3'UTR (IRAK1 Human)
   

   Use

   Luciferase

   Expression

   Mammalian

   Mutation

   4-bp substitutions in the miR-146 target sites

   Available Since

   June 6, 2007

   Availability

   Academic Institutions and Nonprofits only

5. pcDNA5/FRT/TO V5 DNAJB8

   Plasmid

   #19531

   Depositor

   Harm Kampinga

   Article

   Hageman et al Cell Stress Chaperones. 2009 Jan;14(1):1

   Insert

   DnaJ (Hsp40) homolog, subfamily B, member 8 (DNAJB8 Human)
   

   Tags

   V5

   Expression

   Mammalian

   Available Since

   Oct. 6, 2008

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. α1Ic-HE2-pcDNA3

   Plasmid

   #45808

   Depositor

   Edward Perez-Reyes

   Article

   Murbartian et al FEBS Lett. 2002 Sep 25;528(1-3):272-8.

   Insert

   α1Ic (Cacna1i Rat)
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   June 24, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pcDNA3.1 TREK-2

   Plasmid

   #224769

   Purpose

   In vitro transcription for Oocyte expression of 1 TREK-2

   Depositor

   Dan Minor

   Article

   Deal et al Cell Chem Biol. 2024 Jul 18;31(7):1305-1

   Insert

   Potassium channel subfamily member 10 (Kcnk10 Mouse)
   

   Use

   In vitro transcription for oocyte expression

   Promoter

   T7

   Available Since

   Sept. 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pcDNA3.1 V5 His-TOPO-GluCl optbeta-m-YFP Y182F RSR_AAA (Opt β-Gluclv2.0)

   Plasmid

   #47542

   Purpose

   engineered receptor improves heteromeric receptor expression and sensitivity to IVM.

   Depositor

   Henry Lester

   Article

   Frazier et al J Biol Chem. 2013 May 29.

   Insert

   Glutamate-gated chloride channels
   

   Expression

   Mammalian

   Mutation

   retention motif in ER (starting at R340) changed …

   Promoter

   CMV

   Available Since

   Aug. 28, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. LLP787_SnoopCatcher-EZH2-FL-CO

   Plasmid

   #211780

   Purpose

   SnoopTag counterpart binding domain, SnoopCatcher, fused to polycomb repressive complex subunit EZH2, with GFP selection

   Depositor

   Ryan Lister

   Article

   Swain et al Nucleic Acids Res. 2023 Nov 24:gkad1108.

   Insert

   aGCN4-DNMT3A
   

   Tags

   3xTy1

   Expression

   Mammalian

   Mutation

   Last 300 bp codon-optimised (CO) to detect DNMT3A…

   Promoter

   pEF1a and pSV40

   Available Since

   Dec. 15, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pFX-mito-at pHluorin

    Plasmid

    #174183

    Purpose

    Express EYFP with mitochondria-targeting sequence in mammalian cells

    Depositor

    Yusuke Ohba

    Article

    Kashiwagi et al Cell Struct Funct. 2019 Dec 26;44(2):183

    Insert

    at pHluorin with mitchondria-targeting sequence
    

    Tags

    the mitochondria-targeting sequence of the cytoch…

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. TRAF6mut-UTR

    Plasmid

    #15094

    Depositor

    David Baltimore

    Article

    Taganov et al Proc Natl Acad Sci U S A. 2006 Aug 15. 1

    Insert

    TRAF6 3'UTR (TRAF6 Human)
    

    Use

    Luciferase

    Expression

    Mammalian

    Mutation

    4-bp substitutions in the miR-146 target sites

    Available Since

    June 6, 2007

    Availability

    Academic Institutions and Nonprofits only

12. pcDNA5/FRT/TO V5 DNAJB7

    Plasmid

    #19530

    Depositor

    Harm Kampinga

    Article

    Hageman et al Cell Stress Chaperones. 2009 Jan;14(1):1

    Insert

    DnaJ (Hsp40) homolog, subfamily B, member 7 (DNAJB7 Human)
    

    Tags

    V5

    Expression

    Mammalian

    Available Since

    Oct. 6, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pcDNA3.1(+)-FluoSTEP-JNKAR

    Plasmid

    #181857

    Purpose

    Genetically encoded FRET-based sensor for monitoring JNK kinase activity near a protein of interest. Must pair with GFP11-tagged POI to reconstitute donor fluorescence.

    Depositor

    Jin Zhang

    Article

    Tenner et al Sci Adv. 2021 May 21;7(21). pii: 7/21/ea

    Insert

    FluoSTEP-JNKAR
    

    Tags

    mRuby2 and sfGFP(1-10)

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    June 2, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pcDNA3.1(+)-FluoSTEP-AktAR

    Plasmid

    #181856

    Purpose

    Genetically encoded FRET-based sensor for monitoring Akt activity near a protein of interest. Must pair with GFP11-tagged POI to reconstitute donor fluorescence.

    Depositor

    Jin Zhang

    Article

    Tenner et al Sci Adv. 2021 May 21;7(21). pii: 7/21/ea

    Insert

    FluoSTEP-AktAR
    

    Tags

    mRuby2 and sfGFP(1-10)

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    May 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. sq5

    Plasmid

    #24499

    Depositor

    Bruce Conklin

    Article

    Conklin et al Mol Pharmacol. 1996 Oct . 50(4):885-90.

    Insert

    Gs alpha (Gnas Rat)
    

    Tags

    HA (see comments)

    Expression

    Mammalian

    Mutation

    This is Gs alpha with the C-terminal amino acids …

    Available Since

    Aug. 24, 2010

    Availability

    Academic Institutions and Nonprofits only

16. Cav2.2e[a10,Δ18a, Δ24a, 31a, 37b, 46]

    Plasmid

    #26567

    Depositor

    Diane Lipscombe

    Article

    Lin et al Neuron. 1997 Jan . 18(1):153-66.

    Insert

    calcium channel alpha-1B subunit (Cacna1b Rat)
    

    Tags

    6XHis and V5

    Expression

    Mammalian

    Mutation

    Contains exons a10, 31a, 37b, and 46. Deletion…

    Available Since

    Nov. 29, 2010

    Availability

    Academic Institutions and Nonprofits only

17. pACEBac1-PSMB5-PSMB6-PSMB7

    Plasmid

    #217898

    Purpose

    Insect cell expression of human 20S CP proteasome subunit beta type-5 (PSMB5), beta type-6 (PSMB6), and beta type-7 (PSMB7)

    Depositor

    Brenda Schulman

    Article

    Adolf et al Nat Struct Mol Biol. 2024 Apr 10. doi: 1

    Inserts

    PSMB5 (PSMB5 Human)
    PSMB6 (PSMB6 Human)
    PSMB7 (PSMB7 Human)
    

    Expression

    Insect

    Available Since

    May 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. Cav2.2e[Δa10, Δ18a, Δ24a, 31a, 37a, 46]

    Plasmid

    #26569

    Depositor

    Diane Lipscombe

    Article

    Bell et al Neuron. 2004 Jan 8. 41(1):127-38.

    Insert

    calcium channel alpha-1B subunit (Cacna1b Rat)
    

    Tags

    6XHis and V5

    Expression

    Mammalian

    Mutation

    Deletion of exons a10, 18a, and 24a. Contains …

    Available Since

    Nov. 29, 2010

    Availability

    Academic Institutions and Nonprofits only

19. pCosSAD1AprR

    Plasmid

    #190786

    Purpose

    An E. coli cloning vector for the integration of heterologous genes into a linear plasmid pSA3239 in Streptomyces lavendulae subsp. lavendulae CCM 3239 through homologous recombination

    Depositor

    Jan Kormanec

    Article

    Novakova et al Appl Microbiol Biotechnol. 2022 Nov;106(

    Inserts

    upstream region of the aur1 cluster for auricin
    ampramycin resistance cassette
    downstream region of the aur1 cluster for auricin
    

    Expression

    Bacterial

    Available Since

    Oct. 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. pcDNA3-Q769gp120-8his

    Plasmid

    #100929

    Purpose

    Mammalian expression plasmid for gp120 subunit from the Q769.d22 HIV-1 strain

    Depositor

    Erik Procko

    Article

    Heredia et al J Virol. 2019 Mar 20. pii: JVI.00219-19.

    Insert

    HIV-1 (Q769.d22) Env
    

    Tags

    CD5 leader sequence and Purification tag after K5…

    Expression

    Mammalian

    Mutation

    Codon-optimized synthetic gene

    Promoter

    CMV

    Available Since

    March 25, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. YIp128-CUP1-NUbo-GFP-His6

    Plasmid

    #212790

    Purpose

    Copper-inducible expression of the artificial test substrate NUbo-GFP-His6 in budding yeast

    Depositor

    Helle Ulrich

    Article

    Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

    Insert

    NUbo-GFP-His6
    

    Use

    Integrative vector

    Expression

    Yeast

    Promoter

    CUP1

    Available Since

    Jan. 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. Cav2.2e[Δa10, Δ18a, 24a, Δ31a, 37b, 46]

    Plasmid

    #26571

    Depositor

    Diane Lipscombe

    Article

    Lin et al Neuron. 1997 Jan . 18(1):153-66.

    Insert

    calcium channel alpha-1B subunit (Cacna1b Rat)
    

    Tags

    6XHis and V5

    Expression

    Mammalian

    Mutation

    Deletion of exons a10, 18a, and 31a Contains e…

    Available Since

    Nov. 29, 2010

    Availability

    Academic Institutions and Nonprofits only

23. pcDNA5/FRT/TO V5 DNAJB4

    Plasmid

    #19526

    Depositor

    Harm Kampinga

    Article

    Hageman et al Cell Stress Chaperones. 2009 Jan;14(1):1

    Insert

    DnaJ (Hsp40) homolog, subfamily B, member 4 (DNAJB4 Human)
    

    Tags

    V5

    Expression

    Mammalian

    Available Since

    Oct. 6, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. p164 Dync1i1.B

    Plasmid

    #26435

    Depositor

    Elizabeth Fisher

    Article

    Kuta et al PLoS One. 2010 . 5(7):e11682.

    Insert

    cytoplasmic dynein 1 intermediate chain 1 isoform B (Dync1i1 Mouse)
    

    Use

    Subcloning and sequencing

    Mutation

    N/A

    Available Since

    March 21, 2011

    Availability

    Academic Institutions and Nonprofits only

25. pcDNA3.1-alpha4 wt (Hygro)

    Plasmid

    #80017

    Purpose

    Mammalian expression of human integrin alpha4, Hygro selection

    Depositor

    Chinten James Lim

    Article

    Liu et al Mol Cell Biol. 2013 Nov;33(21):4334-45.

    Insert

    integrin alpha4 (ITGA4 Human)
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    July 22, 2016

    Availability

    Academic Institutions and Nonprofits only

26. pBbB8k-csg-NbMS2

    Plasmid

    #171701

    Purpose

    Arabinose-inducible csgBACEFG operon for curli fiber synthesis in which curli subunit CsgA has been fused to a nanobody targeting the coat protein of bacteriophage MS2 via a flexible linker.

    Depositor

    Neel Joshi

    Article

    Birnbaum et al ACS ES T Water. 2022 Nov 11;2(11):1836-1

    Insert

    csgBACEFG, with nanobody fused to CsgA
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Available Since

    June 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pMA7

    Plasmid

    #79967

    Purpose

    TM-MAGE strain

    Depositor

    Morten Sommer

    Article

    Lennen et al Nucleic Acids Res. 2016 Feb 29;44(4):e36

    Inserts

    Lambda Red recombinase beta subunit
    DNA adenine methylase
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Promoter

    pBAD

    Available Since

    Sept. 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pGG-TurboID-GFP-PTS1

    Plasmid

    #209406

    Purpose

    Transiently expressing and visualizing the subcellular localization of TurboID fused with a peroxisomal targeting signal type I (PTS1) in planta

    Depositor

    Rosa Lozano-Durán

    Article

    Tan et al J Integr Plant Biol. 2024 Jan 31. doi: 1

    Insert

    3XHA-TurboID-PTS1
    

    Tags

    GFP

    Expression

    Plant

    Mutation

    TurboID gene sequence C249A

    Promoter

    Cauliflower mosaic virus (CaMV) 35S promoter

    Available Since

    Jan. 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. Dynactin-4/p62-3XHA

    Plasmid

    #31058

    Depositor

    Vann Bennett

    Article

    Ayalon et al J Biol Chem. 2011 Mar 4. 286(9):7370-8.

    Insert

    Dynactin-4 (DCTN4 Human)
    

    Tags

    3XHA

    Expression

    Mammalian

    Available Since

    March 5, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. Tom70NTD-ABHD16Acyto-mNG

    Plasmid

    #195159

    Purpose

    mammalian expression of outer mitochondrial membrane localized ABHD16A tagged with mNeonGreen

    Depositor

    Gia Voeltz

    Article

    Nguyen et al Elife. 2022 Nov 30;11:e84279. doi: 10.75

    Insert

    Tom70NTD (TOMM70 Human)
    

    Tags

    ABHD16A cytoplasmic domain and mNeonGreen

    Expression

    Mammalian

    Mutation

    None

    Promoter

    CMV

    Available Since

    Feb. 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. DCT.VV.Orf37

    Plasmid

    #12725

    Purpose

    This plasmid was designed for screening purposes and may contain discrepancies relative to the current canonical GenBank version of the gene.

    Depositor

    Jon Yewdell, Jack Bennink

    Article

    Tscharke et al J Exp Med. 2005 January 3; 201(1): 95–10

    Insert

    VV.Orf37
    

    Expression

    Mammalian

    Available Since

    Dec. 1, 2006

    Availability

    Academic Institutions and Nonprofits only

32. DCT.VV.Orf48

    Plasmid

    #12736

    Purpose

    This plasmid was designed for screening purposes and may contain discrepancies relative to the current canonical GenBank version of the gene.

    Depositor

    Jon Yewdell, Jack Bennink

    Article

    Tscharke et al J Exp Med. 2005 January 3; 201(1): 95–10

    Insert

    VV.Orf48
    

    Expression

    Mammalian

    Available Since

    Dec. 1, 2006

    Availability

    Academic Institutions and Nonprofits only

33. pQTEV-COPE

    Plasmid

    #31585

    Depositor

    Konrad Buessow

    Article

    Bussow et al Microb Cell Fact. 2005 Jul 5;4:21. doi:

    Insert

    coatomer protein complex, subunit epsilon (COPE Human)
    

    Tags

    His and TEV

    Expression

    Bacterial

    Available Since

    Nov. 20, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. LLP790_αGCN4-EZH2-FL-CO

    Plasmid

    #211784

    Purpose

    SunTag counterpart binding domain, aGCN4, fused to polycomb repressive complex subunit EZH2, with GFP selection

    Depositor

    Ryan Lister

    Article

    Swain et al Nucleic Acids Res. 2023 Nov 24:gkad1108.

    Insert

    SnoopCatcher-KRAB
    

    Tags

    2xOLLAS

    Expression

    Mammalian

    Mutation

    Last 300 bp codon-optimised (CO) to detect KRAB e…

    Promoter

    pEF1a and pSV40

    Available Since

    Dec. 15, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. Cav2.2e[Δa10, Δ18a, Δ24a, 31a, 37b, 46]

    Plasmid

    #26570

    Depositor

    Diane Lipscombe

    Article

    Lin et al Neuron. 1997 Jan . 18(1):153-66.

    Insert

    calcium channel alpha-1B subunit (Cacna1b Rat)
    

    Tags

    6XHis and V5

    Expression

    Mammalian

    Mutation

    Deletion of exons a10, 18a, and 24a. Contains …

    Available Since

    Nov. 29, 2010

    Availability

    Academic Institutions and Nonprofits only

36. p199 Dync1i2.C (Ex1a)

    Plasmid

    #26449

    Depositor

    Elizabeth Fisher

    Article

    Kuta et al PLoS One. 2010 . 5(7):e11682.

    Insert

    cytoplasmic dynein 1 intermediate chain 2 isoform C (exon 1a) (Dync1i2 Mouse)
    

    Use

    Subcloning and sequencing

    Mutation

    N/A

    Available Since

    March 24, 2011

    Availability

    Academic Institutions and Nonprofits only

37. pcDNA5/FRT/TO HIS DNAJB8

    Plasmid

    #19558

    Depositor

    Harm Kampinga

    Article

    Hageman et al Cell Stress Chaperones. 2009 Jan;14(1):1

    Insert

    DnaJ (Hsp40) homolog, subfamily B, member 8 (DNAJB8 Human)
    

    Tags

    His

    Expression

    Mammalian

    Available Since

    Oct. 6, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. b2-TS3

    Plasmid

    #103877

    Purpose

    full-length human b2 with tension sensor at b2_733

    Depositor

    Timothy Springer

    Article

    Nordenfelt et al Nat Commun. 2016 Oct 10;7:13119. doi: 10

    Insert

    full-length human b2 with tension sensor at b2_733 (ITGB2 Human)
    

    Tags

    tension sensor module

    Available Since

    Dec. 1, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pEGFPm-DAD M1041A

    Plasmid

    #25411

    Depositor

    Arthur Alberts

    Article

    Alberts et al J Biol Chem. 2001 Jan 26. 276(4):2824-30

    Insert

    Diap3 (Diaph3 Mouse)
    

    Tags

    EGFP, His, and Myc

    Expression

    Mammalian

    Mutation

    Alanine substitution at critical residue in DAD t…

    Available Since

    Oct. 6, 2014

    Availability

    Academic Institutions and Nonprofits only

40. DCT.VV.Orf42

    Plasmid

    #12730

    Purpose

    This plasmid was designed for screening purposes and may contain discrepancies relative to the current canonical GenBank version of the gene.

    Depositor

    Jon Yewdell, Jack Bennink

    Article

    Tscharke et al J Exp Med. 2005 January 3; 201(1): 95–10

    Insert

    VV.Orf42
    

    Expression

    Mammalian

    Available Since

    Dec. 1, 2006

    Availability

    Academic Institutions and Nonprofits only