We narrowed to 18,263 results for: Met;

1. AB.pCCL.sin.cPPT.U6.miR-17-Decoy.hPGK.GFP.WPRE

   Plasmid

   #46594

   Depositor

   Brian Brown

   Article

   Mullokandov et al Nat Methods. 2012 Jul 1;9(8):840-6. doi:

   Insert

   miR-17 Decoy (MIR17, Mir17 Mouse, Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   hU6

   Available since

   Aug. 29, 2013

   Availability

   Academic Institutions and Nonprofits only

2. ALC1-c005

   Plasmid

   #173092

   Purpose

   Expression of recombinant fusion protein in E. coli

   Depositor

   Lari Lehtiö

   Article

   Sowa et al Cell Rep Methods. 2021 Dec 20;1(8):10012

   Insert

   CHD1L (CHD1L Human)
   

   Use

   Tags

   mCerulean

   Expression

   Bacterial

   Mutation

   L613-K879, codon optimized

   Promoter

   Available since

   Jan. 13, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. Lenti-sgApc/Cre

   Plasmid

   #89641

   Purpose

   Expresses an Apc-targeting gRNA and Cre-recombinase

   Depositor

   Monte Winslow

   Article

   Rogers et al Nat Methods. 2017 May 22. doi: 10.1038/n

   Insert

   gRNA targeting Apc (Apc Mouse)
   

   Use

   Cre/Lox and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 19, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. MacroH2A1.1-c002

   Plasmid

   #173087

   Purpose

   Expression of recombinant fusion protein in E. coli

   Depositor

   Lari Lehtiö

   Article

   Sowa et al Cell Rep Methods. 2021 Dec 20;1(8):10012

   Insert

   MACROH2A1 (MACROH2A1 Human)
   

   Use

   Tags

   mCerulean

   Expression

   Bacterial

   Mutation

   Q162-N369, codon optimized

   Promoter

   Available since

   Dec. 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCMV-SNAP-CRY2-VHH(GFP)

   Plasmid

   #58370

   Purpose

   For use in light-induced inactivation of GFP fused protein through the interaction with CIB1-MP

   Depositor

   Won Do Heo

   Article

   Lee et al Nat Methods. 2014 Jun;11(6):633-6. doi:

   Insert

   CRY2[1-498] (CRY2 Mustard Weed)
   

   Use

   Tags

   SNAP and vhhGFP4

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Sept. 30, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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6. MacroH2A1.2-c002

   Plasmid

   #173088

   Purpose

   Expression of recombinant fusion protein in E. coli

   Depositor

   Lari Lehtiö

   Article

   Sowa et al Cell Rep Methods. 2021 Dec 20;1(8):10012

   Insert

   MACROH2A1 (MACROH2A1 Human)
   

   Use

   Tags

   mCerulean

   Expression

   Bacterial

   Mutation

   Q162-N372, codon optimized

   Promoter

   Available since

   Dec. 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pBbE1k-3

   Plasmid

   #71722

   Purpose

   tyrosine hydroxylase from mouse, dihydropterine reductase from human, pterin-4a-carbinolamine dehydratase from human

   Depositor

   Taek Soon Lee

   Article

   Satoh et al Metab Eng. 2012 Nov;14(6):603-10. doi: 1

   Inserts

   dihydropterine reductase (QDPR Human)
   pterin-4a-carbinolamine dehydratase (PCBD1 Human)
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   pTrc

   Available since

   April 13, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. anti-Notch2_B9-pBIOCAM5

   Plasmid

   #39348

   Depositor

   John McCafferty

   Article

   Falk et al Methods. 2012 Sep;58(1):69-78. doi: 10.1

   Insert

   scFv-Fc-fusion specific for Notch2 (clone B9) (NOTCH2, Notch2 Mouse, Human)
   

   Use

   Tags

   3xFLAG, 6xHis, and human Fc

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Oct. 30, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCry2PHR-mCh-Rac1

   Plasmid

   #42954

   Purpose

   Expresses optogenetically controlled Rac1

   Depositor

   David Schaffer

   Article

   Bugaj et al Nat Methods. 2013 Feb 3. doi: 10.1038/nm

   Insert

   Cry2PHR-mCherry-Rac1 (RAC1 Human, Mustard Weed)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   April 9, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pRSFDuet-1_VNp15-StefinA

    Plasmid

    #182405

    Purpose

    Bacterial expression of Vesicle Nucleating peptide15-stefinA fusion

    Depositor

    Dan Mulvihill

    Article

    Eastwood et al Cell Rep Methods. 2023 Feb 2;3(2):100396

    Insert

    VNp15-stefin (CSTA Human)
    

    Use

    Tags

    Vesicle Nucleating peptide 15 (VNp15)

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Feb. 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAS_4xEcoLeuT(CUA)_EF1_sfGFP150TAG

    Plasmid

    #174892

    Purpose

    amber suppression reporter sfGFP150TAG with EcoLeuT(CUA) amber suppressor tRNA cassette

    Depositor

    Simon Elsaesser

    Article

    Meineke et al Cell Rep Methods. 2023 Nov 20;3(11):1006

    Insert

    sfGFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    150TAG in sfGFP

    Promoter

    EF1

    Available since

    Nov. 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pAAV-CaMKII-iGECI

    Plasmid

    #160423

    Purpose

    AAV plasmid for CaMKII-driven expression of a genetically-encoded calcium indicator iGECI

    Depositor

    Vladislav Verkhusha

    Article

    Shemetov et al Nat Biotechnol. 2021 Mar;39(3):368-377.

    Insert

    iGECI
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CaMKII

    Available since

    Nov. 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. AB.pCCL.sin.cPPT.U6.miR-29b-Decoy.hPGK.GFP.WPRE

    Plasmid

    #46605

    Depositor

    Brian Brown

    Article

    Mullokandov et al Nat Methods. 2012 Jul 1;9(8):840-6. doi:

    Insert

    miR-29b Decoy (MIR29B1, Mir29b-1 Mouse, Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    hU6

    Available since

    Aug. 29, 2013

    Availability

    Academic Institutions and Nonprofits only

14. 3xFLAG-dCas9/pMSCVneo

    Plasmid

    #134982

    Purpose

    Expresses 3xFLAG-dCas9 in mammalian cells for enChIP analysis to purify specific genomic regions of interest.

    Depositor

    Hodaka Fujii

    Article

    Yuno et al Biol Methods Protoc. 2021 Jul 9;6(1):bpa

    Insert

    3xFLAG-dCas9
    

    Use

    CRISPR and Retroviral

    Tags

    3xFLAG tag and NLS

    Expression

    Mammalian

    Mutation

    human codon-optimized, D10A + H840A

    Promoter

    LTR

    Available since

    April 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. ARH3-c002

    Plasmid

    #173095

    Purpose

    Expression of recombinant fusion protein in E. coli

    Depositor

    Lari Lehtiö

    Article

    Sowa et al Cell Rep Methods. 2021 Dec 20;1(8):10012

    Insert

    ADPRS (ADPRS Human)
    

    Use

    Tags

    mCerulean

    Expression

    Bacterial

    Mutation

    A14-S363

    Promoter

    Available since

    Dec. 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. Lenti-sgCdkn2a/Cre

    Plasmid

    #89644

    Purpose

    Expresses a Cdkn2a-targeting gRNA and Cre-recombinase

    Depositor

    Monte Winslow

    Article

    Rogers et al Nat Methods. 2017 May 22. doi: 10.1038/n

    Insert

    gRNA targeting Cdkn2a (Cdkn2a Mouse)
    

    Use

    Cre/Lox and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 19, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pLentiCRISPR-v1-sgHK2_2

    Plasmid

    #163461

    Purpose

    lentiviral vector expressing Cas9 and an sgRNA targeting HK2

    Depositor

    Jason Cantor

    Article

    Rossiter et al Cell Metab. 2021 Feb 23. pii: S1550-4131

    Insert

    sgRNA 2 targeting HK2 (HK2 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Jan. 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pAAV-CAGGS-Flex/5'USS-GFP

    Plasmid

    #197884

    Purpose

    Can be used to generate AAV virus that will express GFP in the presence of Cre and the leakage expression is reduced without Cre

    Depositor

    Kazuto Kobayashi

    Article

    Matsushita et al Cell Rep Methods. 2023 Jan 18;3(2):10039

    Insert

    GFP
    

    Use

    AAV

    Tags

    Expression

    Mutation

    N/A

    Promoter

    CAG

    Available since

    April 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pCMV-MtAcKRS (human-opti)

    Plasmid

    #164195

    Purpose

    To express a Methanosarcina thermophila derived AcKRS in mammalian cells for genetic code expansion

    Depositor

    Tao Liu

    Article

    Hu et al ACS Synth Biol. 2020 Oct 16;9(10):2723-2

    Insert

    MtAcKRS-478(human-opti)
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    D76G; L325M; L329I; Y330F; L333A; C372F

    Promoter

    CMV

    Available since

    Feb. 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. Lenti-sgKeap1/Cre

    Plasmid

    #89645

    Purpose

    Expresses a Keap1-targeting gRNA and Cre-recombinase

    Depositor

    Monte Winslow

    Article

    Rogers et al Nat Methods. 2017 May 22. doi: 10.1038/n

    Insert

    gRNA targeting Keap1 (Keap1 Mouse)
    

    Use

    Cre/Lox and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 19, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge