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c-myc promoter (Del 3)

Plasmid

#16603

Depositor

Bert Vogelstein

Article

He et al Science. 1998 Sep 4. 281(5382):1509-12.

Insert

c-myc promoter (Del 3) (MYC Human)

Use

Luciferase

Tags

luciferase

Expression

Mammalian

Mutation

Del-3 (AccI->PvuII). The AccI site is at -741 …

Available Since

March 24, 2008

Availability

Academic Institutions and Nonprofits only
TB:5xNF- κβ_RE:MiniP

Plasmid

#133878

Purpose

5 copies of the NF-κβ DNA binding motif

Depositor

Koen Venken

Article

Sarrion-Perdigones et al Curr Protoc Mol Biol. 2020 Jun;131(1):e1

Insert

5 copies of the NF-kb DNA binding motif

Use

Luciferase and Synthetic Biology

Expression

Mammalian

Available Since

June 17, 2020

Availability

Academic Institutions and Nonprofits only

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pCS2_Firefly_degron-60A

Plasmid

#200396

Purpose

A plasmid IVT template containing a destabilized Firefly luciferase-PEST (degron) fusion followed by a template-encoded poly(A) tail. Linearization at an Esp3i site.

Depositor

Xiao Wang

Article

Aditham et al ACS Chem Biol. 2022 Dec 16;17(12):3352-3

Insert

Firefly luciferase - degron

Expression

Mammalian

Available Since

July 26, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-hSynapsin1-axon-GCaMP6s

Plasmid

#111262

Purpose

For enriched expression of GCaMP6s in axons

Depositor

Lin Tian

Article

Broussard et al Nat Neurosci. 2018 Sep;21(9):1272-1280.

Has Service

AAV1

Insert

axon-GCaMP6s

Use

AAV

Tags

GAP43 palmitoylation domain

Expression

Mammalian

Promoter

hSynapsin1

Available Since

June 26, 2018

Availability

Academic Institutions and Nonprofits only

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AsCpf1-2NLS

Plasmid

#102565

Purpose

bacterial expression of AsCpf1-2NLS

Depositor

Jennifer Doudna

Article

Moreno-Mateos et al Nat Commun. 2017 Dec 8;8(1):2024. doi: 1

Insert

AsCpf1

Tags

6xHis-MBP-TEV and HA-2xSV40NLS

Expression

Bacterial

Promoter

T7

Available Since

Dec. 21, 2017

Availability

Academic Institutions and Nonprofits only

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pCR1063

Plasmid

#111091

Purpose

Expresses dCas9-KRAB tagged with mCherry: pHR-hEF1a-dCas9-mcherry-KRAB-mPGK-Hyg

Depositor

Jacob Corn

Article

Richardson et al Nat Genet. 2018 Aug;50(8):1132-1139. doi

Insert

dCas9-KRAB

Use

Lentiviral

Tags

mCherry

Available Since

Oct. 18, 2018

Availability

Academic Institutions and Nonprofits only

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KA1396_pGL4-UBCmin-luc2/Klf4-E'

Plasmid

#124187

Purpose

Luciferase reporter driven by the Klf4 enhancer

Depositor

Hans Schöler

Article

Adachi et al Cell Stem Cell. 2018 Aug 2;23(2):266-275

Insert

Klf4 enhancer

Use

Luciferase

Promoter

UBCmin

Available Since

March 27, 2019

Availability

Academic Institutions and Nonprofits only

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KA2626_pGL4-UBCmin-luc2/Klf2-D'

Plasmid

#124199

Purpose

Luciferase reporter driven by the Klf2 enhancer

Depositor

Hans Schöler

Article

Adachi et al Cell Stem Cell. 2018 Aug 2;23(2):266-275

Insert

Klf2 enhancer

Use

Luciferase

Promoter

UBCmin

Available Since

April 19, 2019

Availability

Academic Institutions and Nonprofits only

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KA1401_pGL4-UBCmin-luc2/Klf4-F'

Plasmid

#124188

Purpose

Luciferase reporter driven by the Klf4 enhancer

Depositor

Hans Schöler

Article

Adachi et al Cell Stem Cell. 2018 Aug 2;23(2):266-275

Insert

Klf4 enhancer

Use

Luciferase

Promoter

UBCmin

Available Since

March 27, 2019

Availability

Academic Institutions and Nonprofits only

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KA1395_pGL4-UBCmin-luc2/Klf4-B'

Plasmid

#124186

Purpose

Luciferase reporter driven by the Klf4 enhancer

Depositor

Hans Schöler

Article

Adachi et al Cell Stem Cell. 2018 Aug 2;23(2):266-275

Insert

Klf4 enhancer

Use

Luciferase

Promoter

UBCmin

Available Since

March 27, 2019

Availability

Academic Institutions and Nonprofits only

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SNM-Luc

Plasmid

#35156

Depositor

Linda Penn

Article

Facchini et al Mol Cell Biol. 1997 Jan;17(1):100-14.

Insert

Myc promoter (MYC Human)

Use

Luciferase

Tags

Luciferase

Expression

Mammalian

Mutation

Contains 502 bp of the myc promoter

Promoter

c-myc

Available Since

Oct. 23, 2012

Availability

Academic Institutions and Nonprofits only
pTRIPZ-shWT1_B

Plasmid

#217637

Purpose

Dox inducible shRNA targeting WT-1

Depositor

Constanze Bonifer

Article

Potluri et al Cell Rep. 2021 Apr 20;35(3):109010. doi:

Insert

shWT1_B (WT1 Human)

Use

RNAi

Available Since

May 22, 2024

Availability

Academic Institutions and Nonprofits only

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P445-FBP_17

Plasmid

#139708

Purpose

T-DNA encoding Fungal Bioluminescent Pathway Components, complete pathway, Luz promoter is flower specific, circadian controlled

Depositor

Daniel Voytas

Article

Khakhar et al Elife. 2020 Mar 25;9. pii: 52786. doi: 1

Insert

pSlH4:NnCPH:tSlH4, pSlRBCS2:AsNpga:tAtuG7, pAtuMas:NnH3H:tAtuMas, p35S:NnHisps:t35S, pPhODO1:NnLuz:tAtuOcs

Use

Luciferase

Expression

Plant

Promoter

various

Available Since

May 29, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA5/FRT/TO-EGFP-RIP2

Plasmid

#131201

Purpose

mammalian expression, tet R

Depositor

Thomas Kufer

Article

Ellwanger et al Life Sci Alliance. 2019 Jul 26;2(4). pii

Insert

RIPK2 (RIPK2 Human)

Expression

Mammalian

Promoter

pCMV

Available Since

Sept. 9, 2019

Availability

Academic Institutions and Nonprofits only

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pFRLuc-CoV2-WT

Plasmid

#177614

Purpose

Dual luciferase reporter for SARS-CoV-2 programmed -1 ribosomal frameshifting

Depositor

Junjie Guo

Article

Sun et al Proc Natl Acad Sci U S A. 2021 Jun 29;11

Insert

SARS-CoV-2 FSE

Use

Luciferase

Expression

Mammalian

Available Since

Dec. 10, 2021

Availability

Academic Institutions and Nonprofits only

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pAAV-DIO-TVA-V5-RG

Plasmid

#119743

Purpose

Expresses TVA, V5, and RG in a cre dependent manner.

Depositor

Marie Carlén

Article

Ahrlund-Richter et al Nat Neurosci. 2019 Apr;22(4):657-668. do

Insert

TVA, V5, Rabies Glycoprotein

Use

AAV and Cre/Lox

Tags

V5

Available Since

April 26, 2019

Availability

Academic Institutions and Nonprofits only

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TB:7xE-Box:MiniP

Plasmid

#133880

Purpose

7 copies of the E-box motif

Depositor

Koen Venken

Article

Sarrion-Perdigones et al Curr Protoc Mol Biol. 2020 Jun;131(1):e1

Insert

7 copies of the E-box motif

Use

Luciferase and Synthetic Biology

Expression

Mammalian

Available Since

June 17, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 HisC EXO1b

Plasmid

#68268

Purpose

Mammalian expression of human EXO1

Depositor

Stefano Ferrari

Article

El-Shemerly et al Cancer Res. 2005 May 1;65(9):3604-9.

Insert

EXO1 (EXO1 Human)

Tags

His

Expression

Mammalian

Mutation

b form, codons 1-846. Please see depositor commen…

Promoter

CMV

Available Since

Aug. 12, 2015

Availability

Academic Institutions and Nonprofits only
pSNRW-Z5-Nluc

Plasmid

#174487

Purpose

Recombinant fusion protein gene combining IgG-Fc binding domain Z (5 repeats) of Staphylococcal Protein A and bioluminescence reporter Nanoluciferase in pET28a bacterial expression plasmid.

Depositor

Richard C. Willson

Article

Nandy et al Anal Biochem. 2022 Oct 18:114929. doi: 1

Insert

Z5-Nluc

Tags

6x Histidine tag (N terminal on insert)

Expression

Bacterial

Promoter

T7lac promoter

Available Since

Oct. 25, 2022

Availability

Academic Institutions and Nonprofits only

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BB1_12_pAOX1

Plasmid

#98514

Purpose

BB1 with insert: promoter PAOX1

Depositor

Brigitte Gasser, Diethard Mattanovich, Michael Sauer

Article

Prielhofer et al BMC Syst Biol. 2017 Dec 8;11(1):123. doi

Insert

AOX1 promoter

Expression

Bacterial

Mutation

Internal BsaI and BpiI sites removed from insert

Available Since

Feb. 20, 2018

Availability

Academic Institutions and Nonprofits only

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