We narrowed to 18,334 results for: met

1. pAAV-CaMKII-iGECI

   Plasmid

   #160423

   Purpose

   AAV plasmid for CaMKII-driven expression of a genetically-encoded calcium indicator iGECI

   Depositor

   Vladislav Verkhusha

   Article

   Shemetov et al Nat Biotechnol. 2021 Mar;39(3):368-377.

   Insert

   iGECI
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CaMKII

   Available Since

   Nov. 13, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAS_4xEcoLeuT(CUA)_EF1_sfGFP150TAG

   Plasmid

   #174892

   Purpose

   amber suppression reporter sfGFP150TAG with EcoLeuT(CUA) amber suppressor tRNA cassette

   Depositor

   Simon Elsaesser

   Article

   Meineke et al Cell Rep Methods. 2023 Nov 20;3(11):1006

   Insert

   sfGFP
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   150TAG in sfGFP

   Promoter

   EF1

   Available Since

   Nov. 7, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. AB.pCCL.sin.cPPT.U6.miR-29b-Decoy.hPGK.GFP.WPRE

   Plasmid

   #46605

   Depositor

   Brian Brown

   Article

   Mullokandov et al Nat Methods. 2012 Jul 1;9(8):840-6. doi:

   Insert

   miR-29b Decoy (MIR29B1, Mir29b-1 Mouse, Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   hU6

   Available Since

   Aug. 29, 2013

   Availability

   Academic Institutions and Nonprofits only

4. Lenti-sgCdkn2a/Cre

   Plasmid

   #89644

   Purpose

   Expresses a Cdkn2a-targeting gRNA and Cre-recombinase

   Depositor

   Monte Winslow

   Article

   Rogers et al Nat Methods. 2017 May 22. doi: 10.1038/n

   Insert

   gRNA targeting Cdkn2a (Cdkn2a Mouse)
   

   Use

   Cre/Lox and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   July 19, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. ARH3-c002

   Plasmid

   #173095

   Purpose

   Expression of recombinant fusion protein in E. coli

   Depositor

   Lari Lehtiö

   Article

   Sowa et al Cell Rep Methods. 2021 Dec 20;1(8):10012

   Insert

   ADPRS (ADPRS Human)
   

   Use

   Tags

   mCerulean

   Expression

   Bacterial

   Mutation

   A14-S363

   Promoter

   Available Since

   Dec. 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. 3xFLAG-dCas9/pMSCVneo

   Plasmid

   #134982

   Purpose

   Expresses 3xFLAG-dCas9 in mammalian cells for enChIP analysis to purify specific genomic regions of interest.

   Depositor

   Hodaka Fujii

   Article

   Yuno et al Biol Methods Protoc. 2021 Jul 9;6(1):bpa

   Insert

   3xFLAG-dCas9
   

   Use

   CRISPR and Retroviral

   Tags

   3xFLAG tag and NLS

   Expression

   Mammalian

   Mutation

   human codon-optimized, D10A + H840A

   Promoter

   LTR

   Available Since

   April 22, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. Lenti-sgKeap1/Cre

   Plasmid

   #89645

   Purpose

   Expresses a Keap1-targeting gRNA and Cre-recombinase

   Depositor

   Monte Winslow

   Article

   Rogers et al Nat Methods. 2017 May 22. doi: 10.1038/n

   Insert

   gRNA targeting Keap1 (Keap1 Mouse)
   

   Use

   Cre/Lox and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   July 19, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAAV-CAGGS-Flex/5'USS-GFP

   Plasmid

   #197884

   Purpose

   Can be used to generate AAV virus that will express GFP in the presence of Cre and the leakage expression is reduced without Cre

   Depositor

   Kazuto Kobayashi

   Article

   Matsushita et al Cell Rep Methods. 2023 Jan 18;3(2):10039

   Insert

   GFP
   

   Use

   AAV

   Tags

   Expression

   Mutation

   N/A

   Promoter

   CAG

   Available Since

   April 12, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pLentiCRISPR-v1-sgHK2_2

   Plasmid

   #163461

   Purpose

   lentiviral vector expressing Cas9 and an sgRNA targeting HK2

   Depositor

   Jason Cantor

   Article

   Rossiter et al Cell Metab. 2021 Feb 23. pii: S1550-4131

   Insert

   sgRNA 2 targeting HK2 (HK2 Human)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available Since

   Jan. 26, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCMV-MtAcKRS (human-opti)

    Plasmid

    #164195

    Purpose

    To express a Methanosarcina thermophila derived AcKRS in mammalian cells for genetic code expansion

    Depositor

    Tao Liu

    Article

    Hu et al ACS Synth Biol. 2020 Oct 16;9(10):2723-2

    Insert

    MtAcKRS-478(human-opti)
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    D76G; L325M; L329I; Y330F; L333A; C372F

    Promoter

    CMV

    Available Since

    Feb. 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAS_4xBstTyrT(CUA)_EF1 sfGFP150TAG

    Plasmid

    #174891

    Purpose

    amber suppression reporter sfGFP150TAG expression, with BstTyr(CUA) amber suppressor tRNA cassette

    Depositor

    Simon Elsaesser

    Article

    Meineke et al Cell Rep Methods. 2023 Nov 20;3(11):1006

    Insert

    sfGFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    150TAG in sfGFP

    Promoter

    EF1

    Available Since

    Nov. 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. Bxb1_miRFP703

    Plasmid

    #183759

    Purpose

    Bxb1-specific donor plasmid for intergrating a transcription unit for expression of membrane-localised miRFP703

    Depositor

    Richard Davis

    Article

    Blanch-Asensio et al Cell Rep Methods. 2022 Sep 22;2(10):1003

    Insert

    miRFP703
    

    Use

    Tags

    Lck lipidation signal

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Sept. 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. ALC1-c007

    Plasmid

    #173112

    Purpose

    Expression of recombinant fusion protein in E. coli

    Depositor

    Lari Lehtiö

    Article

    Sowa et al Cell Rep Methods. 2021 Dec 20;1(8):10012

    Insert

    CHD1L (CHD1L Human)
    

    Use

    Tags

    Nanoluc

    Expression

    Bacterial

    Mutation

    L613-K879, codon optimized

    Promoter

    Available Since

    Dec. 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. Str-STIM1-NN_ST-SBP-mCherry

    Plasmid

    #65263

    Purpose

    synchronize trafficking of ST from the ER (RUSH system)

    Depositor

    Franck Perez

    Article

    Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi

    Insert

    Str-STIM1-NN and truncated sialyltransferase (ST6GAL1 Human)
    

    Use

    Tags

    Streptavidin Binding Protein (SBP) and mCherry

    Expression

    Mammalian

    Mutation

    contains only amino acids 1 to 43 of ST

    Promoter

    CMV

    Available Since

    June 19, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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15. pET-3c-rhVTN-C

    Plasmid

    #30224

    Depositor

    James Thomson

    Article

    Chen et al Nat Methods. 2011 May;8(5):424-9. doi: 1

    Insert

    rhVitronectin with C terminus cleavage (VTN Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    deleted amino acids: 399-478

    Promoter

    Available Since

    May 24, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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16. PA-mCherry1-Vimentin-7

    Plasmid

    #57202

    Purpose

    Localization: Intermediate Filaments, Excitation: 400 / 504, Emission: 515 / 517

    Depositor

    Michael Davidson

    Article

    York et al Nat Methods. 2011 Apr;8(4):327-33. doi:

    Insert

    Vimentin (VIM Human)
    

    Use

    Tags

    PA-mCherry1

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Jan. 13, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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17. pLenti-CaMKIIa-eArch 3.0-EYFP

    Plasmid

    #35514

    Purpose

    Lentiviral expression of CaMKIIa-driven Arch 3.0 for optical inhibition.

    Depositor

    Karl Deisseroth

    Article

    Mattis et al Nat Methods. 2011 Dec 18;9(2):159-72. do

    Insert

    eArch3.0-EYFP
    

    Use

    Lentiviral

    Tags

    EYFP

    Expression

    Mammalian

    Mutation

    Enhanced trafficking signal and ER export signal …

    Promoter

    CaMKIIa

    Available Since

    May 2, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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18. pmScarlet-H_alphaTubulin_C1

    Plasmid

    #85046

    Purpose

    In vivo visualization of microtubules (can be used for colocalization studies)

    Depositor

    Dorus Gadella

    Article

    Bindels et al Nat Methods. 2016 Nov 21. doi: 10.1038/n

    Insert

    Alpha-Tubulin (TUBA1B Human)
    

    Use

    Tags

    mScarlet-H

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Dec. 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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19. pENTR-mCherry-attP(C31)-*BleoR

    Plasmid

    #183752

    Purpose

    phiC31 landing pad cassette.

    Depositor

    Richard Davis

    Article

    Blanch-Asensio et al Cell Rep Methods. 2022 Sep 22;2(10):1003

    Insert

    FRT-mCherry-attP-BleoR-F3
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Sept. 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pLVPT-GDNF-rtTR-KRAB-2SM2

    Plasmid

    #11647

    Depositor

    Patrick Aebischer, Didier Trono

    Article

    Szulc et al Nat Methods. 2006 Feb;3(2):109-16. doi:

    Insert

    mPGK, GDNF, rtTR-KRAB-2SM2, Tet-Off (GDNF Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Aug. 17, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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