Addgene

We narrowed to 23,156 results for: Nov

Showing: 9751 - 9800 of 23156 results

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pCAG-RFP-p2a-postASAP

Plasmid

#178793

Purpose

Expresses voltage sensor targeted to dendrites and spines along with RFP

Depositor

Rafael Yuste

Article

Cornejo et al Science. 2022 Jan 7;375(6576):82-86. doi

Insert

FingR.PSD95, ASAP2f and TagRFP

Use

Tags

TagRFP

Expression

Mammalian

Mutation

mutations in amino acids L146G, S147T N149R, S150…

Promoter

CAG

Available since

April 19, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pmyc-GFP-TNRC6C

Plasmid

#42023

Depositor

Kumiko Ui-Tei

Article

Nishi et al RNA. 2013 Jan;19(1):17-35. doi: 10.1261/

Insert

TNRC6C (TNRC6C Human)

Use

Tags

EGFP and Myc

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 5, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
TOP-H2B-YFP-DD

Plasmid

#96891

Purpose

Reporter to monitor translation of 5'TOP mRNA

Depositor

Tobias Meyer

Article

Han et al Nat Methods. 2014 Jan;11(1):86-93. doi:

Insert

TOP-H2B-YFP-DD

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 9, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
B4-4

Plasmid

#111478

Purpose

translational release factor 1

Depositor

Cheemeng Tan

Article

Villarreal et al Nat Chem Biol. 2018 Jan;14(1):29-35. doi

Insert

translational release factor 1

Use

Tags

6xHis

Expression

Bacterial

Mutation

Promoter

Available since

June 18, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pEB1-moxGFP

Plasmid

#103984

Purpose

Plasmid encoding moxGFP

Depositor

Philippe Cluzel

Article

Balleza et al Nat Methods. 2018 Jan;15(1):47-51. doi:

Insert

moxGFP

Use

Low copy

Tags

Expression

Bacterial

Mutation

Mutations relative to wild-type GFP (S30R, Y39N, …

Promoter

proC

Available since

Dec. 13, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
Gi-c010

Plasmid

#173080

Purpose

Expression of recombinant fusion protein in E. coli

Depositor

Lari Lehtiö

Article

Sowa et al Cell Rep Methods. 2021 Dec 20;1(8):10012

Insert

GAP

Use

Tags

mCitrine

Expression

Bacterial

Mutation

mCitrine + last 10 residues of GNA1, codon optimi…

Promoter

Available since

Dec. 9, 2021

Availability

Academic Institutions and Nonprofits only

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Flag-mRunx1c

Plasmid

#64894

Purpose

Lentiviral expression of Flag-tagged murine transcription factor Runx1c

Depositor

Georges Lacaud

Article

Batta et al Cell Rep. 2014 Dec 11;9(5):1871-84. doi:

Insert

Runx1c (Runx1 Mouse)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 9, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
GST-PRMT6

Plasmid

#34700

Depositor

Steven Clarke

Article

Frankel et al J Biol Chem. 2002 Feb 1;277(5):3537-43.

Insert

PMRT6

Use

Tags

GST

Expression

Bacterial

Mutation

Promoter

tac

Available since

Jan. 27, 2012

Availability

Academic Institutions and Nonprofits only
TOP-H2B-mCherry-DD

Plasmid

#96894

Purpose

Reporter to monitor translation of 5'TOP mRNA

Depositor

Tobias Meyer

Article

Han et al Nat Methods. 2014 Jan;11(1):86-93. doi:

Insert

TOP-H2B-mCherry-DD

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 9, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pTetR193-Wasabi

Plasmid

#86105

Purpose

mWasabi reporter gene gated by temperature-sensitive TetR I193N

Depositor

Mikhail Shapiro

Article

Piraner et al Nat Chem Biol. 2017 Jan;13(1):75-80. doi

Inserts

TetRts
mWasabi

Use

Tags

6xHis

Expression

Bacterial

Mutation

I193N

Promoter

ptetA and ptetR

Available since

Feb. 27, 2017

Availability

Academic Institutions and Nonprofits only

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BB1_23_dCAS9(3xFLAG_SV40NLS)

Plasmid

#161478

Purpose

BB1 encoding a FLAG tagged dCAS9 with a SV40 NLS

Depositor

Diethard Mattanovich, Brigitte Gasser

Article

Baumschabl et al ACS Synth Biol. 2020 Dec 18;9(12):3202-3

Insert

dCAS9

Use

CRISPR and Synthetic Biology

Tags

FLAG Tag, SV40 NLS

Expression

Mutation

Promoter

Available since

March 15, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
p177bp-LacOx50-Hyg

Plasmid

#210213

Purpose

Integrates 50xLacO repeats with a hygromycin drug selection marker into 177bp repeat region.

Depositor

Calvin Tiengwe

Article

Budzak et al Mol Biochem Parasitol. 2023 Dec;256:1115

Insert

50xLacO repeats derived from: PMID: 12864855.

Use

Tags

Expression

Mutation

Promoter

Available since

Nov. 27, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pAD6

Plasmid

#134349

Purpose

E. coli mfd gene cloned into pSKB2 with an N-terminal, ppx-cleavable His6 tag

Depositor

Seth Darst

Article

Deaconescu et al Acta Crystallogr Sect F Struct Biol Crys

Insert

mfd (mfd E. coli)

Use

Tags

His

Expression

Bacterial

Mutation

Promoter

T7

Available since

Dec. 10, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
B1-4

Plasmid

#111466

Purpose

translational elongation factor Tu

Depositor

Cheemeng Tan

Article

Villarreal et al Nat Chem Biol. 2018 Jan;14(1):29-35. doi

Insert

translational elongation factor Tu

Use

Tags

6xHis

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 14, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
B4-1

Plasmid

#111475

Purpose

Ala-tRNA synthetase

Depositor

Cheemeng Tan

Article

Villarreal et al Nat Chem Biol. 2018 Jan;14(1):29-35. doi

Insert

Ala-tRNA synthetase

Use

Tags

6xHis

Expression

Bacterial

Mutation

Promoter

Available since

June 18, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
B7-5

Plasmid

#111493

Purpose

translational initiation factor 1

Depositor

Cheemeng Tan

Article

Villarreal et al Nat Chem Biol. 2018 Jan;14(1):29-35. doi

Insert

translational initiation factor 1

Use

Tags

6xHis

Expression

Bacterial

Mutation

Promoter

Available since

June 18, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
B1-5

Plasmid

#111467

Purpose

translational elongation factor Ts

Depositor

Cheemeng Tan

Article

Villarreal et al Nat Chem Biol. 2018 Jan;14(1):29-35. doi

Insert

translational elongation factor Ts

Use

Tags

6xHis

Expression

Bacterial

Mutation

Promoter

Available since

Dec. 19, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
B1-3

Plasmid

#111465

Purpose

translational release factor 3

Depositor

Cheemeng Tan

Article

Villarreal et al Nat Chem Biol. 2018 Jan;14(1):29-35. doi

Insert

translational release factor 3

Use

Tags

6xHis

Expression

Bacterial

Mutation

Promoter

Available since

Jan. 8, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
B3-2

Plasmid

#111472

Purpose

translational elongation factor 4

Depositor

Cheemeng Tan

Article

Villarreal et al Nat Chem Biol. 2018 Jan;14(1):29-35. doi

Insert

translational elongation factor 4

Use

Tags

6xHis

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 14, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
B7-1

Plasmid

#111489

Purpose

translational elongation factor G

Depositor

Cheemeng Tan

Article

Villarreal et al Nat Chem Biol. 2018 Jan;14(1):29-35. doi

Insert

translational elongation factor G

Use

Tags

6xHis

Expression

Bacterial

Mutation

Promoter

Available since

April 1, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pMVV098

Plasmid

#215304

Purpose

Expresses sfGFP under induction with the DAPG Marionette Cassette (PhIFAM + PPhIF-sfGFP) with a BBR1 backbone.

Depositor

Brian Pfleger

Article

Venkataraman et al ACS Synth Biol. 2023 Dec 15;12(12):3623-

Insert

sfGFP

Use

Synthetic Biology

Tags

None

Expression

Bacterial

Mutation

Promoter

PPhIF

Available since

July 25, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pEB2-DsRed-Express

Plasmid

#103995

Purpose

Plasmid encoding DsRed-Express

Depositor

Philippe Cluzel

Article

Balleza et al Nat Methods. 2018 Jan;15(1):47-51. doi:

Insert

DsRed-Express

Use

Low copy

Tags

Expression

Bacterial

Mutation

Mutations relative to DsRed (R2A, K5E, N6D, T21S,…

Promoter

proC

Available since

Dec. 27, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pAGT5439

Plasmid

#192351

Purpose

L0 MoClo-compatible GoldenGate-module of the OCS terminator (tOCS) with stop

Depositor

Tom Schreiber

Article

Schindele et al Plant Biotechnol J. 2023 Mar;21(3):457-4

Insert

TTCG_Stop-tOCS_CGCT

Use

Tags

Expression

Plant

Mutation

Promoter

Available since

Nov. 17, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pAGT9403

Plasmid

#192352

Purpose

L0 MoClo-compatible GoldenGate-module of the NOS terminator (tNOS) with stop

Depositor

Tom Schreiber

Article

Schindele et al Plant Biotechnol J. 2023 Mar;21(3):457-4

Insert

TTCG_Stop-tNOS_CGCT

Use

Tags

Expression

Plant

Mutation

Promoter

Available since

Nov. 17, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
B5-5

Plasmid

#111484

Purpose

translational initiation factor 3

Depositor

Cheemeng Tan

Article

Villarreal et al Nat Chem Biol. 2018 Jan;14(1):29-35. doi

Insert

translational initiation factor 3

Use

Tags

6xHis

Expression

Bacterial

Mutation

Promoter

Available since

June 19, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
B3-1

Plasmid

#111471

Purpose

translational initiation factor 2

Depositor

Cheemeng Tan

Article

Villarreal et al Nat Chem Biol. 2018 Jan;14(1):29-35. doi

Insert

translational initiation factor 2

Use

Tags

6xHis

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 13, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
B4-5

Plasmid

#111479

Purpose

ribosome recycling factor

Depositor

Cheemeng Tan

Article

Villarreal et al Nat Chem Biol. 2018 Jan;14(1):29-35. doi

Insert

ribosome recycling factor

Use

Tags

6xHis

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 13, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pEB1-mCerulean3

Plasmid

#103973

Purpose

Plasmid encoding mCerulean3

Depositor

Philippe Cluzel

Article

Balleza et al Nat Methods. 2018 Jan;15(1):47-51. doi:

Insert

mCerulean3

Use

Low copy

Tags

Expression

Bacterial

Mutation

Mutations relative to wild-type GFP (F64L, Y66W, …

Promoter

proC

Available since

Dec. 13, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pVITRO1-mCherry-NES

Plasmid

#186349

Purpose

Cytosolic mCherry expression in mammalian cells

Depositor

Chuan-Hsiang Huang

Article

Yang et al Cell. 2021 Dec 9;184(25):6193-6206.e14.

Insert

mCherry-NES

Use

Tags

Expression

Mammalian

Mutation

WT

Promoter

Rat EF1α

Available since

July 14, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pVITRO1-BFP-NLS

Plasmid

#184458

Purpose

Nucleus-targeted EBFP2 expression in mammalian cells

Depositor

Chuan-Hsiang Huang

Article

Yang et al Cell. 2021 Dec 9;184(25):6193-6206.e14.

Insert

EBFP2-3xNLS

Use

Tags

Expression

Mammalian

Mutation

WT

Promoter

Rat EF1α

Available since

May 26, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pVITRO1-BFP-CAAX

Plasmid

#184459

Purpose

Plasma membrane-targeted EBFP2 expression in mammalian cells

Depositor

Chuan-Hsiang Huang

Article

Yang et al Cell. 2021 Dec 9;184(25):6193-6206.e14.

Insert

EBFP2-CAAX

Use

Tags

Expression

Mammalian

Mutation

WT

Promoter

Rat EF1α

Available since

May 26, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pEB2-Katushka

Plasmid

#104008

Purpose

Plasmid encoding Katushka

Depositor

Philippe Cluzel

Article

Balleza et al Nat Methods. 2018 Jan;15(1):47-51. doi:

Insert

Katushka

Use

Low copy

Tags

Expression

Bacterial

Mutation

Mutations relative to eqFP578 (E3V, K6T, N21D, R3…

Promoter

proC

Available since

Dec. 27, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pVITRO1-BFP-LaminB1

Plasmid

#184460

Purpose

Nuclear membrane-targeted EBFP2 expression in mammalian cells

Depositor

Chuan-Hsiang Huang

Article

Yang et al Cell. 2021 Dec 9;184(25):6193-6206.e14.

Insert

EBFP2-LaminB1

Use

Tags

Expression

Mammalian

Mutation

WT

Promoter

Rat EF1α

Available since

May 26, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
perbB1-Citrine

Plasmid

#40266

Depositor

Martin Offterdinger

Article

Offterdinger et al Traffic. 2008 Jan;9(1):147-55. Epub 2007

Insert

erbB1 (EGFR Human)

Use

Tags

Citrine

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Nov. 26, 2012

Availability

Academic Institutions and Nonprofits only

Enlarge
pSA101

Plasmid

#59787

Purpose

drives ZIF-1 protein in C. elegans neurons

Depositor

Jeremy Nance

Article

Armenti et al Development. 2014 Dec;141(23):4640-7. do

Inserts

rab-3 promoter
zif-1 coding (zif-1 Nematode)
unc-54 3' UTR

Use

Tags

Expression

Mutation

Promoter

Available since

Oct. 29, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
pEB1-T-Sapphire

Plasmid

#103977

Purpose

Plasmid encoding T-Sapphire

Depositor

Philippe Cluzel

Article

Balleza et al Nat Methods. 2018 Jan;15(1):47-51. doi:

Insert

T-Sapphire

Use

Low copy

Tags

Expression

Bacterial

Mutation

Mutations relative to wild-type GFP (Q69M, C70V, …

Promoter

proC

Available since

Dec. 13, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pCAG-postASAP-p2a-ChrimsonR

Plasmid

#178794

Purpose

Expresses voltage sensor targeted to dendrites and spines along with ChrimsonR

Depositor

Rafael Yuste

Article

Cornejo et al Science. 2022 Jan 7;375(6576):82-86. doi

Insert

FingR.PSD95, ASAP2f and ChrimsonR

Use

Tags

mRuby2

Expression

Mammalian

Mutation

mutations in amino acids L146G, S147T N149R, S150…

Promoter

CAG

Available since

Dec. 10, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
hAur-A D274A

Plasmid

#8511

Depositor

Joan Ruderman

Article

Crane et al J Cell Sci 2004 Dec 1;117(Pt 25):5975-83

Insert

hAur-A (AURKA Human)

Use

Retroviral

Tags

Expression

Mammalian

Mutation

D274A, catalytically dead mutant

Promoter

Available since

Dec. 7, 2005

Availability

Academic Institutions and Nonprofits only

Enlarge
pUG-248

Plasmid

#104709

Purpose

Cloning Module

Depositor

Marco Trujillo

Article

Kowarschik et al New Phytol. 2018 Mar;217(4):1749-1763. d

Insert

PUB22 C13A (PUB22 Mustard Weed)

Use

Synthetic Biology

Tags

Expression

Mutation

C13A, BpiI/BsaI sites removed by point-mutation

Promoter

Available since

Feb. 1, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pUG-21

Plasmid

#104707

Purpose

Cloning Module

Depositor

Marco Trujillo

Article

Kowarschik et al New Phytol. 2018 Mar;217(4):1749-1763. d

Insert

PUB22 WT (PUB22 Mustard Weed)

Use

Synthetic Biology

Tags

Expression

Mutation

BpiI/BsaI sites removed by point-mutation

Promoter

Available since

Feb. 1, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pUG-UBC1

Plasmid

#104666

Purpose

Cloning Module

Depositor

Marco Trujillo

Article

Kowarschik et al New Phytol. 2018 Mar;217(4):1749-1763. d

Insert

AtUBC1 (UBC1 Mustard Weed)

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Feb. 1, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pCMV-EPN-11

Plasmid

#79926

Purpose

Palm1-Late1-I3-01-myc

Depositor

Neil King

Article

Votteler et al Nature. 2016 Dec 8;540(7632):292-295. do

Insert

EPN-11

Use

Tags

Expression

Bacterial and Mammalian

Mutation

Promoter

Available since

Feb. 10, 2017

Availability

Academic Institutions and Nonprofits only
pLacI241-Wasabi

Plasmid

#86102

Purpose

mWasabi reporter gene gated by temperature-sensitive LacI A241T

Depositor

Mikhail Shapiro

Article

Piraner et al Nat Chem Biol. 2017 Jan;13(1):75-80. doi

Inserts

LacIts
mWasabi

Use

Tags

6xHis

Expression

Bacterial

Mutation

A241T

Promoter

T7A1O4/O3 and pLacI

Available since

Feb. 24, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pAG306-GPD-AVT1 chr I

Plasmid

#41898

Purpose

Yeast expression of AVT1.

Depositor

Dan Gottschling

Article

Hughes et al Nature. 2012 Dec 13;492(7428):261-5. doi

Insert

AVT1 (AVT1 Budding Yeast)

Use

Tags

Expression

Yeast

Mutation

Promoter

GPD

Available since

Feb. 7, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
pEB1-mClover3

Plasmid

#103994

Purpose

Plasmid encoding mClover3

Depositor

Philippe Cluzel

Article

Balleza et al Nat Methods. 2018 Jan;15(1):47-51. doi:

Insert

mClover3

Use

Low copy

Tags

Expression

Bacterial

Mutation

Mutations relative to wild-type GFP (S30R, Y39N, …

Promoter

proC

Available since

Dec. 14, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
prNFL-EGFP

Plasmid

#132605

Purpose

Expresses EGFP-tagged rat NFL

Depositor

Anthony Brown

Article

Stone et al Cytoskeleton (Hoboken). 2019 Jul;76(7-8)

Insert

Nefl (Nefl Rat)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Nov. 20, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA4TO-hCALML3

Plasmid

#47601

Depositor

Emanuel Strehler

Article

Bennett et al J Biol Chem. 2007 Feb 2;282(5):3205-12.

Insert

CALM3 (CALML3 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

tet-inducible CMV

Available since

Aug. 27, 2013

Availability

Academic Institutions and Nonprofits only
YIplac211-MNN9-msGFP2

Plasmid

#177105

Purpose

Expresses Mnn9-msGFP2 in yeast cells

Depositor

Benjamin Glick

Article

Casler et al J Cell Biol. 2022 Jan 3;221(1). pii: 212

Insert

MNN9 (MNN9 Budding Yeast)

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

Nov. 17, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
XRCC1-c003

Plasmid

#173090

Purpose

Expression of recombinant fusion protein in E. coli

Depositor

Lari Lehtiö

Article

Sowa et al Cell Rep Methods. 2021 Dec 20;1(8):10012

Insert

XRCC1 (XRCC1 Human)

Use

Tags

mCerulean

Expression

Bacterial

Mutation

E315-P406

Promoter

Available since

Dec. 9, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pET29b-EPN-01*

Plasmid

#80423

Purpose

For expression of EPN-01* in E. coli

Depositor

Wesley Sundquist

Article

Votteler et al Nature. 2016 Dec 8;540(7632):292-295. do

Insert

EPN-01*

Use

Tags

Myc

Expression

Bacterial

Mutation

Promoter

Available since

Jan. 27, 2017

Availability

Academic Institutions and Nonprofits only

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