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pMXs-hu-c-Myc-dN1

Plasmid

#50773

Purpose

retroviral expression of human c-Myc dN1 fragment

Depositor

Shinya Yamanaka

Article

Nakagawa et al Proc Natl Acad Sci U S A. 2010 Aug 10;10

Insert

c-Myc -dN1 (MYC Human)

Use

Retroviral

Expression

Mammalian

Mutation

N-terminal deletion, contains aa 14-439

Available Since

Feb. 28, 2014

Availability

Academic Institutions and Nonprofits only

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deltaMyc-HA

Plasmid

#16556

Depositor

Bert Vogelstein

Article

Hermeking et al Proc Natl Acad Sci U S A. 2000 Feb 29. 9

Insert

c-MYC (MYC Human)

Tags

HA

Expression

Mammalian

Mutation

Deletion of aa 40-178

Available Since

July 3, 2008

Availability

Academic Institutions and Nonprofits only
pOTTC1619 pAAV SYN1 Nuc-EYFP miR-30 SST

Plasmid

#135565

Purpose

An AAV vector expressing miR-30a shRNA vs rat SOM (aka SST) and a nuclear EYFP reporter

Depositor

Christopher Richie

Article

Venniro et al Proc Natl Acad Sci U S A. 2020 Apr 7;117

Inserts

miR-30 rat SST
Nuc-EYFP

Use

AAV

Tags

3xNLS

Expression

Mammalian

Promoter

SYN1 and hSYN1

Available Since

Sept. 8, 2021

Availability

Academic Institutions and Nonprofits only

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pSicoR-Ef1a-mCh-Puro-Luci

Plasmid

#31850

Depositor

Bruce Conklin

Article

Salomonis et al Proc Natl Acad Sci U S A. 2010 Jun 8;107

Insert

Luciferase RNAi

Use

Cre/Lox, Lentiviral, and RNAi

Expression

Mammalian

Promoter

U6 for shRNA; Ef1alpha for mCherry-puromycin

Available Since

Jan. 3, 2012

Availability

Academic Institutions and Nonprofits only

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HOXB4 (human) HA-TAT-tag pET

Plasmid

#8526

Depositor

Corey Largman

Article

Shen et al Proc Natl Acad Sci U S A 1989 Nov;86(21)

Insert

HOXB4 (HOXB4 Human)

Tags

HA

Expression

Bacterial

Mutation

replace T7 and HIS tags of pET with an HA tag;

Available Since

May 30, 2006

Availability

Academic Institutions and Nonprofits only
pGEX-2TK cyclin T1 (1-250) (P#467)

Plasmid

#14606

Depositor

Matija Peterlin

Article

Fujinaga et al Proc Natl Acad Sci U S A. 1999 Feb 16. 9

Insert

cyclin T1 (1-250) (CCNT1 Human)

Tags

GST

Expression

Bacterial

Mutation

amino acids 1-250

Available Since

June 30, 2008

Availability

Academic Institutions and Nonprofits only

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pcDNA3-GFP-LplA(AAG)

Plasmid

#61820

Purpose

Contains GFP tag for direct live-cell fluorescence imaging of the resofurin ligase

Depositor

Alice Ting

Article

Liu et al Proc Natl Acad Sci U S A. 2014 Oct 28;11

Insert

E. coli lipoic acid ligase

Tags

GFP

Expression

Mammalian

Mutation

E20A, F147A, H149G

Promoter

CMV

Available Since

Sept. 18, 2015

Availability

Academic Institutions and Nonprofits only

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CFP-GIV-CT WT

Plasmid

#69532

Purpose

Mammalian expression of The CFP-tagged GIV-CT biosensors

Depositor

Pradipta Ghosh

Article

Midde et al Proc Natl Acad Sci U S A. 2015 Mar 3;112

Insert

GIV (CCDC88A Human)

Tags

CFP

Expression

Mammalian

Mutation

C terminal amino acids S1660-S1870 of human GIV

Promoter

CMV

Available Since

Oct. 19, 2015

Availability

Academic Institutions and Nonprofits only

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p6589 MSCV-IP N-HAonly CHEK2

Plasmid

#34903

Depositor

Peter Howley

Article

White et al Proc Natl Acad Sci U S A. 2012 Jan 9.

Insert

CHEK2 (CHEK2 Human)

Use

Retroviral

Tags

HA

Expression

Mammalian

Promoter

MSCV LTR

Available Since

Feb. 8, 2012

Availability

Academic Institutions and Nonprofits only
MSCV-TAP(FLAG-HA) CYLD

Plasmid

#60031

Purpose

CYLD with N-terminal TAP-tag (HA,FLAG) and IRES-Puro to allow tagged protein to be maintained

Depositor

Stephen Elledge

Article

Stegmeier et al Proc Natl Acad Sci U S A. 2007 May 22. 1

Insert

Cylindromatosis (CYLD Human)

Use

Retroviral

Tags

FLAG, HA, and IRES Puro

Expression

Mammalian

Promoter

LTR-driven expression

Available Since

Feb. 24, 2016

Availability

Academic Institutions and Nonprofits only
pDN-T1TCbt

Plasmid

#44514

Depositor

Gabor Balazsi

Article

Nevozhay et al Proc Natl Acad Sci U S A. 2009 Mar 31;10

Inserts

PGal1-T123 promoter (GAL1 Synthetic, Budding Yeast)
tetR::ymcherry

Use

Synthetic Biology; Expression regulator/reporter

Expression

Yeast

Mutation

Three tandem tet operators (3XtetO2) downstream o…

Promoter

PGal1-T123 promoter

Available Since

April 23, 2013

Availability

Academic Institutions and Nonprofits only

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pCNL-C1_fibrillarin

Plasmid

#65709

Purpose

Expresses CNL-fibrillarin in mammalian cells

Depositor

Yasushi Okada

Article

Takai et al Proc Natl Acad Sci U S A. 2015 Apr 7;112

Insert

fibrillarin (FBL Human)

Use

Luciferase

Tags

CNL

Expression

Mammalian

Promoter

CMV

Available Since

June 22, 2015

Availability

Academic Institutions and Nonprofits only
pGEX4T2 gp78 G2BR mt / JM26

Plasmid

#13308

Depositor

Allan Weissman

Article

Chen et al Proc Natl Acad Sci U S A. 2006 Jan 10. 1

Insert

gp78 (AMFR Human)

Tags

GST

Expression

Bacterial

Mutation

cDNA corresponds to aa309-643 derived from human …

Available Since

Oct. 16, 2006

Availability

Academic Institutions and Nonprofits only
pRShygro shID3#1

Plasmid

#19164

Depositor

Joan Massague

Article

Gupta et al Proc Natl Acad Sci U S A. 2007 Dec 4. 10

Insert

small hairpin RNA against inhibitor of DNA binding 3 (ID3 Human)

Use

RNAi and Retroviral

Expression

Mammalian

Available Since

Sept. 16, 2008

Availability

Academic Institutions and Nonprofits only
pCI-neo-(HA)3-ε

Plasmid

#198531

Purpose

Expression of HA-tagged AP-4 ε in mammalian cells

Depositor

Juan Bonifacino

Article

Mattera et al Proc Natl Acad Sci U S A. 2017 Dec 12;11

Insert

AP-4 ε (AP4E1 Human)

Tags

triple HA tag

Expression

Mammalian

Mutation

silent substitutions in codons 235, 262, 905 and …

Promoter

CMV

Available Since

April 3, 2023

Availability

Academic Institutions and Nonprofits only

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pOTTC1618 pAAV SYN1 Nuc-EYFP miR-30 FF3

Plasmid

#135564

Purpose

An AAV vector expressing miR-30a shRNA vs FF luciferase and a nuclear EYFP reporter

Depositor

Christopher Richie

Article

Venniro et al Proc Natl Acad Sci U S A. 2020 Apr 7;117

Inserts

miR-30 FF3
Nuc-EYFP

Use

AAV

Tags

3xNLS

Expression

Mammalian

Promoter

SYN1 and hSYN1

Available Since

Sept. 8, 2021

Availability

Academic Institutions and Nonprofits only

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J23114-mRFP1-331Bb

Plasmid

#78279

Purpose

mRFP1 behind constitutive promoter J23114 in pSEVA331Bb backbone

Depositor

Tom Ellis

Article

Florea et al Proc Natl Acad Sci U S A. 2016 Jun 14;11

Insert

J23114-mRFP1

Available Since

Nov. 10, 2016

Availability

Academic Institutions and Nonprofits only

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pHR-ZIP(FF)

Plasmid

#27135

Depositor

Ron Vale

Article

Yudushkin et al Proc Natl Acad Sci U S A. 2010 Dec 21;10

Insert

CD3zeta, eGFP, mCherry and ZAP70 (residues 1-259) (ZAP70, Cd247 Mouse, Human)

Use

Lentiviral

Tags

eGFP and mCherry

Expression

Mammalian

Mutation

Tyrosines in ITAMS 1-3 mutated to phenylalanines …

Available Since

April 12, 2011

Availability

Academic Institutions and Nonprofits only

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pDN-G1TCbt

Plasmid

#44515

Depositor

Gabor Balazsi

Article

Nevozhay et al Proc Natl Acad Sci U S A. 2009 Mar 31;10

Inserts

PGal1-D12 promoter (GAL1 Synthetic, Budding Yeast)
tetR::ymcherry

Use

Synthetic Biology; Expression regulator/reporter

Expression

Yeast

Mutation

Two tandem tet operators (2XtetO2) downstream of …

Promoter

PGal1-D12 promoter

Available Since

April 30, 2013

Availability

Academic Institutions and Nonprofits only

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pBabe 3xFLAG mouse Bmf

Plasmid

#17243

Depositor

Joan Brugge

Article

Schmelzle et al Proc Natl Acad Sci U S A. 2007 Mar 6. 10

Insert

Bmf (Bmf Mouse)

Use

Retroviral

Tags

3xFLAG

Expression

Mammalian

Available Since

Jan. 18, 2008

Availability

Academic Institutions and Nonprofits only