We narrowed to 10,444 results for: T7

1. pET28 mid.4

   Plasmid

   #191895

   Purpose

   Encodes a GFP design, mid.4

   Depositor

   Sarel Fleishman

   Article

   Weinstein et al Nat Commun. 2023 May 20;14(1):2890. doi:

   Insert

   mid.4
   

   Use

   Tags

   His Tag

   Expression

   Bacterial

   Mutation

   V68A S72A Y145M L220V

   Promoter

   T7

   Available since

   Dec. 14, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pET28 sf:bleach.3

   Plasmid

   #191925

   Purpose

   Encodes a GFP design, sf:bleach.3

   Depositor

   Sarel Fleishman

   Article

   Weinstein et al Nat Commun. 2023 May 20;14(1):2890. doi:

   Insert

   sf:bleach.3
   

   Use

   Tags

   His Tag

   Expression

   Bacterial

   Mutation

   S72A V112I Y145M T167V H181I V224I

   Promoter

   T7

   Available since

   Dec. 5, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pET28 bleach.3

   Plasmid

   #191914

   Purpose

   Encodes a GFP design, bleach.3

   Depositor

   Sarel Fleishman

   Article

   Weinstein et al Nat Commun. 2023 May 20;14(1):2890. doi:

   Insert

   bleach.3
   

   Use

   Tags

   His Tag

   Expression

   Bacterial

   Mutation

   S72A V112I Y145M T167V H181I V224I

   Promoter

   T7

   Available since

   Dec. 5, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pET28 mid.12

   Plasmid

   #191913

   Purpose

   Encodes a GFP design, mid.12

   Depositor

   Sarel Fleishman

   Article

   Weinstein et al Nat Commun. 2023 May 20;14(1):2890. doi:

   Insert

   mid.12
   

   Use

   Tags

   His Tag

   Expression

   Bacterial

   Mutation

   T65S S72V Y145M T167V H181I V224I

   Promoter

   T7

   Available since

   Dec. 5, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pET28 acid.5

   Plasmid

   #191903

   Purpose

   Encodes a GFP design, acid.5

   Depositor

   Sarel Fleishman

   Article

   Weinstein et al Nat Commun. 2023 May 20;14(1):2890. doi:

   Insert

   acid.5
   

   Use

   Tags

   His Tag

   Expression

   Bacterial

   Mutation

   S72T Y145M T167V H181V

   Promoter

   T7

   Available since

   Dec. 5, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pET28 acid.4

   Plasmid

   #191898

   Purpose

   Encodes a GFP design, acid.4

   Depositor

   Sarel Fleishman

   Article

   Weinstein et al Nat Commun. 2023 May 20;14(1):2890. doi:

   Insert

   acid.4
   

   Use

   Tags

   His Tag

   Expression

   Bacterial

   Mutation

   V61L Q69L Y145M L220V

   Promoter

   T7

   Available since

   Dec. 5, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pET28 acid.3

   Plasmid

   #191897

   Purpose

   Encodes a GFP design, acid.3

   Depositor

   Sarel Fleishman

   Article

   Weinstein et al Nat Commun. 2023 May 20;14(1):2890. doi:

   Insert

   acid.3
   

   Use

   Tags

   His Tag

   Expression

   Bacterial

   Mutation

   T65S Q69L S72A T108V Y145M V224I

   Promoter

   T7

   Available since

   Dec. 5, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pET28 sf:mid.10

   Plasmid

   #191934

   Purpose

   Encodes a GFP design, sf:mid.10

   Depositor

   Sarel Fleishman

   Article

   Weinstein et al Nat Commun. 2023 May 20;14(1):2890. doi:

   Insert

   sf:mid.10
   

   Use

   Tags

   His Tag

   Expression

   Bacterial

   Mutation

   L42V Q69L S72A Y145F T167I L220V V224I

   Promoter

   T7

   Available since

   Dec. 1, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pET28 bleach.1

   Plasmid

   #191885

   Purpose

   Encodes a GFP design, bleach.1

   Depositor

   Sarel Fleishman

   Article

   Weinstein et al Nat Commun. 2023 May 20;14(1):2890. doi:

   Insert

   bleach.1
   

   Use

   Tags

   His Tag

   Expression

   Bacterial

   Mutation

   T108L Y145M T167V L220V

   Promoter

   T7

   Available since

   Dec. 1, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pET28 mid.3

    Plasmid

    #191884

    Purpose

    Encodes a GFP design, mid.3

    Depositor

    Sarel Fleishman

    Article

    Weinstein et al Nat Commun. 2023 May 20;14(1):2890. doi:

    Insert

    mid.3
    

    Use

    Tags

    His Tag

    Expression

    Bacterial

    Mutation

    S72A T108V Y145M T167V H181I V224I

    Promoter

    T7

    Available since

    Dec. 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pET28 thermo.9

    Plasmid

    #191882

    Purpose

    Encodes a GFP design, thermo.9

    Depositor

    Sarel Fleishman

    Article

    Weinstein et al Nat Commun. 2023 May 20;14(1):2890. doi:

    Insert

    thermo.9
    

    Use

    Tags

    His Tag

    Expression

    Bacterial

    Mutation

    T167V T203H E222Q

    Promoter

    T7

    Available since

    Dec. 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pET28 basic.3

    Plasmid

    #191881

    Purpose

    Encodes a GFP design, basic.3

    Depositor

    Sarel Fleishman

    Article

    Weinstein et al Nat Commun. 2023 May 20;14(1):2890. doi:

    Insert

    basic.3
    

    Use

    Tags

    His Tag

    Expression

    Bacterial

    Mutation

    T65S V68A S72A Y145S L220V V224I

    Promoter

    T7

    Available since

    Dec. 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pET28 mid.2

    Plasmid

    #191880

    Purpose

    Encodes a GFP design, mid.2

    Depositor

    Sarel Fleishman

    Article

    Weinstein et al Nat Commun. 2023 May 20;14(1):2890. doi:

    Insert

    mid.2
    

    Use

    Tags

    His Tag

    Expression

    Bacterial

    Mutation

    T108I Y145I

    Promoter

    T7

    Available since

    Dec. 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pET28 bleach.6

    Plasmid

    #191879

    Purpose

    Encodes a GFP design, bleach.6

    Depositor

    Sarel Fleishman

    Article

    Weinstein et al Nat Commun. 2023 May 20;14(1):2890. doi:

    Insert

    bleach.6
    

    Use

    Tags

    His Tag

    Expression

    Bacterial

    Mutation

    V61L S72C Y145M H181L V224I

    Promoter

    T7

    Available since

    Dec. 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pET28 slow.3

    Plasmid

    #191877

    Purpose

    Encodes a GFP design, slow.3

    Depositor

    Sarel Fleishman

    Article

    Weinstein et al Nat Commun. 2023 May 20;14(1):2890. doi:

    Insert

    slow.3
    

    Use

    Tags

    His Tag

    Expression

    Bacterial

    Mutation

    Q69L S72V Y145S T167V L220V V224I

    Promoter

    T7

    Available since

    Dec. 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pET28 basic.2

    Plasmid

    #191911

    Purpose

    Encodes a GFP design, basic.2

    Depositor

    Sarel Fleishman

    Article

    Weinstein et al Nat Commun. 2023 May 20;14(1):2890. doi:

    Insert

    basic.2
    

    Use

    Tags

    His Tag

    Expression

    Bacterial

    Mutation

    S72A V112I Y145V L220V V224I

    Promoter

    T7

    Available since

    Nov. 23, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pET-PLS-KP3-C93A-C96A_rpoA-U

    Plasmid

    #190962

    Purpose

    Test the U-to-C editing activity of KP3:C93A/C96A on AtrpoA editing site, in E. coli

    Depositor

    Bernard Gutmann

    Article

    Ichinose et al Commun Biol. 2022 Sep 15;5(1):968. doi:

    Insert

    PLS-KP3
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    DYW-KP:C93A/C96A

    Promoter

    T7

    Available since

    Oct. 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pET-PLS-KP6-C93A-C96A_rpoA-C

    Plasmid

    #190970

    Purpose

    Test the C-to-U editing activity of KP6:C93A/C96A on AtrpoA editing site, in E. coli

    Depositor

    Bernard Gutmann

    Article

    Ichinose et al Commun Biol. 2022 Sep 15;5(1):968. doi:

    Insert

    PLS-KP6
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    DYW-KP:C93A/C96A

    Promoter

    T7

    Available since

    Oct. 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pET-PLS-KP2-C93A-C96A_rpoA-U

    Plasmid

    #190958

    Purpose

    Test the U-to-C editing activity of KP2:C93A/C96A on AtrpoA editing site, in E. coli

    Depositor

    Bernard Gutmann

    Article

    Ichinose et al Commun Biol. 2022 Sep 15;5(1):968. doi:

    Insert

    PLS-KP2
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    DYW-KP:C93A/C96A

    Promoter

    T7

    Available since

    Oct. 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pET-PLS-KP6-C93A-C96A_rpoA-U

    Plasmid

    #190971

    Purpose

    Test the U-to-C editing activity of KP6:C93A/C96A on AtrpoA editing site, in E. coli

    Depositor

    Bernard Gutmann

    Article

    Ichinose et al Commun Biol. 2022 Sep 15;5(1):968. doi:

    Insert

    PLS-KP6
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    DYW-KP:C93A/C96A

    Promoter

    T7

    Available since

    Oct. 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. TTR C10S E63C

    Plasmid

    #190009

    Purpose

    Expression of human TTR in E coli

    Depositor

    Peter Wright

    Article

    Sun et al J Biol Chem. 2022 Jun 18;298(8):102162.

    Insert

    TTR (TTR Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    C10S, E63C

    Promoter

    T7

    Available since

    Sept. 27, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. mTln1-F2F3-P229L-pET151

    Plasmid

    #177879

    Purpose

    Expresses mouse Talin 1 F2F3-P229L domains in bacterial cells

    Depositor

    Ben Goult

    Article

    Azizi et al Hum Mol Genet. 2022 Jul 21. pii: 6647920

    Insert

    mouse Talin 1 F2F3 P229L mutant (Tln1 Mouse)
    

    Use

    Tags

    His-tag, TEV cleavage site

    Expression

    Bacterial

    Mutation

    changed Proline 229 to Leucine (P229L)

    Promoter

    T7

    Available since

    Sept. 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. mTln1-F2-P229S-pET151

    Plasmid

    #177871

    Purpose

    Expresses mouse Talin 1 F2-P229S domain in bacterial cells

    Depositor

    Ben Goult

    Article

    Azizi et al Hum Mol Genet. 2022 Jul 21. pii: 6647920

    Insert

    mouse Talin 1 F2 P229S mutant (Tln1 Mouse)
    

    Use

    Tags

    His-tag, TEV cleavage site

    Expression

    Bacterial

    Mutation

    changed Proline 229 to Serine (P229S)

    Promoter

    T7

    Available since

    Sept. 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. mTln1-F2-P229L-pET151

    Plasmid

    #177876

    Purpose

    Expresses mouse Talin 1 F2-P229L domain in bacterial cells

    Depositor

    Ben Goult

    Article

    Azizi et al Hum Mol Genet. 2022 Jul 21. pii: 6647920

    Insert

    mouse Talin 1 F2 P229L mutant (Tln1 Mouse)
    

    Use

    Tags

    His-tag, TEV cleavage site

    Expression

    Bacterial

    Mutation

    changed Proline 229 to Leucine (P229L)

    Promoter

    T7

    Available since

    Sept. 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. mTln1-F2F3-P229S-pET151

    Plasmid

    #177878

    Purpose

    Expresses mouse Talin 1 F2F3-P229S domains in bacterial cells

    Depositor

    Ben Goult

    Article

    Azizi et al Hum Mol Genet. 2022 Jul 21. pii: 6647920

    Insert

    mouse Talin 1 F2F3 P229S mutant (Tln1 Mouse)
    

    Use

    Tags

    His-tag, TEV cleavage site

    Expression

    Bacterial

    Mutation

    changed Proline 229 to Serine (P229S)

    Promoter

    T7

    Available since

    Sept. 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pET303-ColE1-T-P110A

    Plasmid

    #180237

    Purpose

    Expresses T domain of Colicin E1 (residues 1-190) with proline to alanine mutation at residue 110

    Depositor

    Joanna Slusky

    Article

    Budiardjo et al Elife. 2022 Feb 24;11. pii: 73297. doi:

    Insert

    Colicin E1
    

    Use

    Tags

    6x His-tag

    Expression

    Bacterial

    Mutation

    Truncation of ColE1 containing T domain of ColE1 …

    Promoter

    T7

    Available since

    Aug. 16, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pET28b-MBP-bhaB5

    Plasmid

    #167812

    Purpose

    Expresses His6- and MBP-tagged BhaB5 in E. coli

    Depositor

    Wilfred van der Donk

    Article

    Daniels et al Nat Chem. 2022 Jan;14(1):71-77. doi: 10.

    Insert

    BhaB5
    

    Use

    Tags

    His6, MBP

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Aug. 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pET-UTX(420-633)-H6

    Plasmid

    #183773

    Purpose

    Express pET-UTX(420-633)-H6 in E.coli. Purified pET-UTX(420-633)-H6 was used for generating antibodies in rabbits.

    Depositor

    Peter Harte

    Article

    Tie et al Mol Cell Biol. 2012 Jun;32(12):2323-34.

    Insert

    UTX
    

    Use

    Tags

    6 His tag at the C-terminus

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    April 21, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pPOLY_new

    Plasmid

    #179276

    Purpose

    Expresses CuCBP, CcCDP and BaSP tricistronically in E. coli BL21(DE3)

    Depositor

    Bernd Nidetzky

    Article

    Schwaiger et al Microb Cell Fact. 2022 Apr 9;21(1):61. d

    Inserts

    cellodextrin phosphorylase
    cellobiose phosphorylase
    sucrose phosphorylase (C8077_RS00435 Bifidobacterium alodescentis)
    

    Use

    Synthetic Biology

    Tags

    6xHIS and Strep-Tag II

    Expression

    Bacterial

    Mutation

    Promoter

    T7 lacO

    Available since

    April 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pFGC-T7RibJ-RRvT

    Plasmid

    #173226

    Purpose

    Encodes RRvT fluorescent protein. To be used in cell-free system

    Depositor

    Jim Haseloff

    Article

    Guzman-Chavez et al ACS Synth Biol. 2022 Mar 18;11(3):1114-1

    Insert

    RRvT
    

    Use

    Cell-free system

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    March 9, 2022

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

31. pJ414-PCY1:S495A

    Plasmid

    #98151

    Purpose

    Expresses PCY1:S495A from Saponaria vaccaria possesing a N-terminal His6-tag (TEV cleavable) in E. coli.

    Depositor

    James Naismith

    Article

    Ludewig et al ACS Chem Biol. 2018 Mar 16;13(3):801-811

    Insert

    Peptide cyclase 1:S495A
    

    Use

    Tags

    His6-TEV

    Expression

    Bacterial

    Mutation

    Changed serine 495 to alanine

    Promoter

    T7

    Available since

    March 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pJ414-PCY1:W603A

    Plasmid

    #98153

    Purpose

    Expresses PCY1:W603A from Saponaria vaccaria possesing a N-terminal His6-tag (TEV cleavable) in E. coli.

    Depositor

    James Naismith

    Article

    Ludewig et al ACS Chem Biol. 2018 Mar 16;13(3):801-811

    Insert

    Peptide cyclase 1:W603A
    

    Use

    Tags

    His6-TEV

    Expression

    Bacterial

    Mutation

    Changed tryptophan 603 to alanine

    Promoter

    T7

    Available since

    March 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pJ414-PCY1:H695Q

    Plasmid

    #98154

    Purpose

    Expresses PCY1:H695Q from Saponaria vaccaria possesing a N-terminal His6-tag (TEV cleavable) in E. coli.

    Depositor

    James Naismith

    Article

    Ludewig et al ACS Chem Biol. 2018 Mar 16;13(3):801-811

    Insert

    Peptide cyclase 1:H695Q
    

    Use

    Tags

    His6-TEV

    Expression

    Bacterial

    Mutation

    Changed histidine 695 to glutamine

    Promoter

    T7

    Available since

    March 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pJ414-PCY1:H695A

    Plasmid

    #98155

    Purpose

    Expresses PCY1H695A from Saponaria vaccaria possesing a N-terminal His6-tag (TEV cleavable) in E. coli.

    Depositor

    James Naismith

    Article

    Ludewig et al ACS Chem Biol. 2018 Mar 16;13(3):801-811

    Insert

    Peptide cyclase 1:H695A
    

    Use

    Tags

    His6-TEV

    Expression

    Bacterial

    Mutation

    Changed histidine 695 to alanine

    Promoter

    T7

    Available since

    March 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pJ414-PCY1:Y696G

    Plasmid

    #98156

    Purpose

    Expresses PCY1:R696G from Saponaria vaccaria possesing a N-terminal His6-tag (TEV cleavable) in E. coli.

    Depositor

    James Naismith

    Article

    Ludewig et al ACS Chem Biol. 2018 Mar 16;13(3):801-811

    Insert

    Peptide cyclase 1:Y696G
    

    Use

    Tags

    His6-TEV

    Expression

    Bacterial

    Mutation

    Changed tyrosine 696 to glycine

    Promoter

    T7

    Available since

    March 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pRSF-G1pCNPRS

    Plasmid

    #174719

    Purpose

    tRNA synthetase/tRNA pair for the in vivo incorporation of L-3-(2-cyano-5-pyridyl)alanine (pCNP), into proteins in E. coli in response to the amber (TAG) codon

    Depositor

    Thomas Huber

    Article

    Abdelkader et al Angew Chem Int Ed Engl. 2022 Jan 31. doi

    Insert

    tRNA synthetase
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Feb. 10, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. NmMetQ C20A

    Plasmid

    #172112

    Purpose

    Expression of Methionine Binding Protein (MetQ) with C20A mutation in signal sequence

    Depositor

    Douglas Rees

    Article

    Sharaf et al Elife. 2021 Aug 19;10. pii: 69742. doi:

    Insert

    Methionine Binding Protein
    

    Use

    Tags

    10x Histidine Tag and N.meningitidis signal seque…

    Expression

    Bacterial

    Mutation

    Changed Proline 22 to Alanine on MetQ and Cystein…

    Promoter

    T7 Promoter

    Available since

    Oct. 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pPodA11

    Plasmid

    #162843

    Purpose

    Expression protein designed PodA10 from a TEV cleavable IPTG inducible plasmid with ampicillin resistance

    Depositor

    Dianne Newman

    Article

    VanDrisse et al Proc Natl Acad Sci U S A. 2021 Mar 23;11

    Insert

    Protein Designed Pyocyanin demethylase
    

    Use

    Tags

    TEV-cleavable 6x-His tag

    Expression

    Bacterial

    Mutation

    A53N, I73T, A87V, M99V, A129T from WT PodA

    Promoter

    T7

    Available since

    Sept. 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pENTR-D-kcnj13

    Plasmid

    #173143

    Purpose

    This gene was cloned into the gateway middle entry vector. It can be used for generating antisense riboprobe or gene expression using a gateway system.

    Depositor

    GuangJun Zhang

    Article

    Silic et al Dev Dyn. 2021 Sep 23. doi: 10.1002/dvdy.

    Insert

    kcnj13 (kcnj13 Zebrafish)
    

    Use

    Pcr cloning vector

    Tags

    Expression

    Mutation

    Promoter

    No promoter at 5 end; T7 promoter at 3 end.

    Available since

    Sept. 27, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pENTR-D-kcnj1b

    Plasmid

    #173126

    Purpose

    This gene was cloned into the gateway middle entry vector. It can be used for generating antisense riboprobe or gene expression using a gateway system.

    Depositor

    GuangJun Zhang

    Article

    Silic et al Dev Dyn. 2021 Sep 23. doi: 10.1002/dvdy.

    Insert

    kcnj1b (kcnj1b Zebrafish)
    

    Use

    Pcr cloning vector

    Tags

    Expression

    Mutation

    Promoter

    No promoter at 5 end; T7 promoter at 3 end.

    Available since

    Sept. 27, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge