Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

Filter By

More Filters

We narrowed to 13,953 results for: FLU

Clear keywords
Showing: 9801 - 9850 of 13953 results

  13. mGFP-hNET-A457P

    Plasmid
    #193365
    Purpose
    expression of mutated human norpinephrine transporter tagged with monomeric GFP at the N-terminus in mammalian cells
    Depositor
    Harald Sitte
    Article
    Luethi et al Commun Biol. 2022 Nov 17;5(1):1259. doi:
    Insert
    human Norepinephrine Transporter (A457P mutation) (SLC6A2 Human)
    Tags
    monomeric GFP (mGFP)
    Expression
    Mammalian
    Mutation
    A457P (GCC to CCC)
    Promoter
    CMV
    Available Since
    Jan. 9, 2023
    Availability
    Academic Institutions and Nonprofits only
    193365-plasmid-map-sequence-id-381451

    Enlarge

  14. mGFP-hNET-R121A/K334A/R440A

    Plasmid
    #193366
    Purpose
    expression of mutated human norpinephrine transporter tagged with monomeric GFP at the N-terminus in mammalian cells
    Depositor
    Harald Sitte
    Article
    Luethi et al Commun Biol. 2022 Nov 17;5(1):1259. doi:
    Insert
    human Norepinephrine Transporter (SLC6A2 Human)
    Tags
    monomeric GFP (mGFP)
    Expression
    Mammalian
    Mutation
    R121A (CGG to GCG) K334A (AAA to GCA) R440A (CGA …
    Promoter
    CMV
    Available Since
    Jan. 9, 2023
    Availability
    Academic Institutions and Nonprofits only
    193366-plasmid-map-sequence-id-381450

    Enlarge

  15. pAAV.hSynap.iAChSnFR-Venus-NULL

    Plasmid
    #137953
    Purpose
    Expresses iAChSnFR (non-binding yellow version) under hSynapsin promoter
    Depositor
    Loren Looger
    Article
    Borden et al bioRxiv 2020
    Insert
    iAChSnFR (yellow version, non-binding variant)
    Use
    AAV
    Expression
    Mammalian
    Mutation
    cpSFGFP replaced with cpSFVenus; Y140A binding si…
    Promoter
    hSynapsin
    Available Since
    Feb. 11, 2020
    Availability
    Academic Institutions and Nonprofits only
    137953-plasmid-map-sequence-id-271684

    Enlarge

  16. pcDNA3.1-nAChr-Alpha6-CFP

    Plasmid
    #50482
    Purpose
    this report is the first to directly measure nAChr subunit stoichiometry using FRET and plasma membrane localization of Alpha6 and Beta3 containing receptors using TIRF
    Depositor
    Henry Lester
    Article
    Drenan et al Mol Pharmacol. 2008 Jan;73(1):27-41. Epu
    Insert
    nAChr-alpha6 (Chrna6 Mouse)
    Tags
    CFP fusion in M3-M4 loop (after residue A405)
    Expression
    Mammalian
    Mutation
    Gly-Ala-Gly flexible linker flanking the CFP open…
    Promoter
    CMV
    Available Since
    Jan. 13, 2014
    Availability
    Academic Institutions and Nonprofits only

  18. pcDNA3.1-SARS2-Spike-HiBiT

    Plasmid
    #191212
    Purpose
    Transient expression in mammalian cells of SARS-CoV-2 (A1) spike protein tagged on the C-terminus with HiBiT, the high affinity small fragment of split nanoluciferase assay
    Depositor
    Mohan Babu
    Article
    Broderick et al Nat Commun. 2025 Jul 1;16(1):5784. doi:
    Insert
    SARS-CoV-2 spike_WT (S SARS CoV-2)
    Tags
    HiBiT (high affinity small nanoluciferase fragmen…
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    April 30, 2025
    Availability
    Academic Institutions and Nonprofits only
    191212-plasmid-map-sequence-id-437009

    Enlarge

  22. pTwist-CMV-HDGFL2_Cryptic_TwinstrepTEV_6xHis

    Plasmid
    #232344
    Purpose
    Expresses human HDGFL2 protein with TDP-43 regulated cryptic exon, along with an N-terminal twinstrep tag + TEV protease site and C-terminal His-tag
    Depositor
    Philip Wong
    Article
    Irwin et al Nat Med. 2024 Feb;30(2):382-393. doi: 10
    Insert
    HDGF Like 2 (HDGFL2 Human)
    Tags
    GGGS linker, 6xHis and Twin-strep, TEV protease s…
    Expression
    Mammalian
    Mutation
    TDP-43 regulated cryptic exon
    Available Since
    Feb. 21, 2025
    Availability
    Academic Institutions and Nonprofits only
    232344-plasmid-map-sequence-id-462328

    Enlarge

  23. pUC19-HDRT-TRAC-CD19.CAR-Cas12a.PAM.mutated

    Plasmid
    #215769
    Purpose
    encodes for HDR template for optimized AsCas12a-mediated knock-in of a CD19-specific CAR into the TRAC locus
    Depositor
    Dimitrios Wagner
    Article
    Glaser et al Genome Biol. 2023 Apr 24;24(1):89. doi:
    Inserts
    TRAC-5' homology arm (TRAC Human)
    P2A-CD19.28.zeta-bgH (CD19 Bovine, Mouse, Human)
    TRAC-3' homology arm (TRAC Human)
    Expression
    Bacterial
    Mutation
    Cas12a PAM mutated
    Available Since
    June 5, 2024
    Availability
    Academic Institutions and Nonprofits only
    215769-plasmid-map-sequence-id-435524

    Enlarge

  24. AICSDP-45: TTN-mEGFP

    Plasmid
    #114412
    Purpose
    Homology arms and linker-mEGFP sequence for C-terminus tagging of human TTN, via Cas9-excisable CAGGS-mCherry selection cassette
    Depositor
    Allen Institute for Cell Science
    Article
    Roberts et al Stem Cell Reports. 2019 May 14;12(5):114
    Insert
    TTN Homology Arms with linker-mEGFP and Cas9-excisable selection cassette (TTN Human)
    Use
    CRISPR; Donor template
    Tags
    linker-mEGFP
    Expression
    Mammalian
    Mutation
    homology arms contain point mutations to disrupt …
    Available Since
    Sept. 5, 2018
    Availability
    Academic Institutions and Nonprofits only
    114412-plasmid-map-sequence-id-220885

    Enlarge

  28. pCEP4-myc-PDGFRA-K627R_EGFP-PEST_reporter

    Plasmid
    #172597
    Purpose
    Mammalian fluorescent (EGFP-PEST) reporter plasmid for PDGFRA-K627R mutant signaling.
    Depositor
    Daniel Kiss
    Article
    Yu et al Hum Mol Genet. 2022 Jul 21;31(14):2348-2
    Insert
    myc-PDGFRA-K627R (PDGFRA Synthetic, Human)
    Use
    Egfp-pest_reporter
    Tags
    Extracellular c-myc epitope tag and Signal peptid…
    Expression
    Mammalian
    Mutation
    PDGFRA-K627R
    Promoter
    CMV
    Available Since
    Oct. 14, 2022
    Availability
    Academic Institutions and Nonprofits only
    172597-plasmid-map-sequence-id-338908

    Enlarge

  30. pDGB1alpha2_KanR_BastaR_Rb7_GFP

    Plasmid
    #186427
    Purpose
    optimisation of phosphinothricin (Basta) selection in plant cells; combines kanamycin resistance gene (nptII) and Basta resistance gene (bar) with fluorescent marker (eGFP)
    Depositor
    Lukas Fischer
    Article
    Kobercova et al Plant Cell Rep. 2023 Jan 7. doi: 10.1007
    Inserts
    KanR
    BastaR
    Rb7
    eGFP
    Use
    Synthetic Biology; Binary vector for escherichia …
    Expression
    Plant
    Mutation
    BsaI and BsmBI sites removed
    Promoter
    CaMV 35S and Pnos
    Available Since
    Jan. 12, 2023
    Availability
    Academic Institutions and Nonprofits only
    186427-plasmid-map-sequence-id-369354

    Enlarge

  33. A3Bi-ctd E255A -Cas9n-UGI-NLS

    Plasmid
    #109429
    Purpose
    Expresses the C-terminal catalytically inactive mutant of human APOBEC3B containing an L1 intron fused to Cas9n, Uracil DNA Glycosylase Inhibitor, with a nuclear localization signal.
    Depositor
    Reuben Harris
    Article
    St Martin et al Nucleic Acids Res. 2018 May 9. pii: 4994
    Insert
    Apolipoprotein mRNA Editing Enzyme, Catalytic Polypeptide-like 3B C-terminal Doman E255A Catalytic Mutant (APOBEC3B Human)
    Use
    CRISPR
    Expression
    Mammalian
    Mutation
    Insertion of an L1 intron into the coding sequenc…
    Promoter
    CMV
    Available Since
    July 2, 2018
    Availability
    Academic Institutions and Nonprofits only
    109429-plasmid-map-sequence-id-212934

    Enlarge

  34. pCEP4-myc-PDGFRA-R914W_EGFP-PEST_reporter

    Plasmid
    #172596
    Purpose
    Mammalian fluorescent (EGFP-PEST) reporter plasmid for PDGFRA-R914W mutant signaling.
    Depositor
    Daniel Kiss
    Article
    Yu et al Hum Mol Genet. 2022 Jul 21;31(14):2348-2
    Insert
    myc-PDGFRA-R914W (PDGFRA Synthetic, Human)
    Use
    Egfp-pest_reporter
    Tags
    Extracellular c-myc epitope tag and Signal peptid…
    Expression
    Mammalian
    Mutation
    PDGFRA-R914W
    Promoter
    CMV
    Available Since
    Oct. 14, 2022
    Availability
    Academic Institutions and Nonprofits only
    172596-plasmid-map-sequence-id-338758

    Enlarge

  37. pET21-10XHis-GST-HRV-Her1-dL5-Her1

    Plasmid
    #73217
    Purpose
    Bacterial expression vector for Z1907-dL5(E52D)-Z1907 affibody fusion protein (AFA) with N-terminal His10, GST and HRV3C cleavage site (MBIC5, dL5**, FAP, AffiFAP)
    Depositor
    Marcel Bruchez
    Article
    Wang et al Bioconjug Chem. 2015 Jan 21;26(1):137-44
    Insert
    10XHis-GST-HRV-Her1-dL5-Her1 (EGFR Schistosoma japonicum, Synthetic, Human)
    Tags
    10XHis-GST-HRV and The Her1 Affibody and dL5 FAP …
    Expression
    Bacterial
    Mutation
    The dL5** FAP is E50D, L89S, also known as E52D/L…
    Promoter
    T7
    Available Since
    March 21, 2016
    Availability
    Academic Institutions and Nonprofits only
    73217-plasmid-map-sequence-id-140443

    Enlarge

  38. pAAV-EF1α1.1-FLEX-rc [Archon1-KGC-GFP-ER2]

    Plasmid
    #115891
    Purpose
    AAV-mediated expression of Archon1-KGC-GFP-ER2 under the EF1α promoter (1.1kb short version), in floxed/reversed (Cre-dependent) manner. Using bGHpA signal.
    Depositor
    Edward Boyden
    Article
    Piatkevich et al Nat Chem Biol. 2018 Feb 26. pii: 10.1038
    Insert
    Archon1-KGC-GFP-ER2
    Use
    AAV
    Tags
    ER2, GFP, and KGC
    Expression
    Mammalian
    Promoter
    EF1α promoter (1.1kb short version)
    Available Since
    July 2, 2019
    Availability
    Academic Institutions and Nonprofits only
    115891-plasmid-map-sequence-id-242072

    Enlarge

  39. pET21-10XHis-GST-HRV-Her1-dL5

    Plasmid
    #73216
    Purpose
    Bacterial expression vector for Z1907-dL5(E52D) affibody fusion protein (AF) with N-terminal His10, GST and HRV3C cleavage site (MBIC5, dL5**, FAP)
    Depositor
    Marcel Bruchez
    Article
    Wang et al Bioconjug Chem. 2015 Jan 21;26(1):137-44
    Insert
    10XHis-GST-HRV-Her1-dL5 (EGFR Schistosoma japonicum, Synthetic, Human)
    Tags
    10XHis-GST-HRV and The Her1 Affibody and dL5 FAP …
    Expression
    Bacterial
    Mutation
    The dL5** FAP is E50D, L89S, also known as E52D/L…
    Promoter
    T7
    Available Since
    March 10, 2016
    Availability
    Academic Institutions and Nonprofits only
    73216-plasmid-map-sequence-id-137065

    Enlarge

  40. pET21-10XHis-GST-HRV-dL5-Her1

    Plasmid
    #73215
    Purpose
    Bacterial expression vector for dL5(E52D)-Z1907 affibody fusion protein (FA) with N-terminal His10, GST and HRV3C cleavage site (MBIC5, dL5**, FAP)
    Depositor
    Marcel Bruchez
    Article
    Wang et al Bioconjug Chem. 2015 Jan 21;26(1):137-44
    Insert
    EGFR (EGFR Schistosoma japonicum, Synthetic, Human)
    Tags
    10XHis-GST-HRV and The dL5 FAP and Her1 affibody …
    Expression
    Bacterial
    Mutation
    The dL5** FAP is E50D, L89S, also known as E52D/L…
    Promoter
    T7
    Available Since
    March 9, 2016
    Availability
    Academic Institutions and Nonprofits only
    73215-plasmid-map-sequence-id-137064

    Enlarge

Showing: 9801 - 9850 of 13953 results